Antiviral activity of lycorine against Zika virus in vivo and in vitro.

Abstract:

:The emergence and re-emergence of Zika virus (ZIKV), is a cause for international concern. These highly pathogenic arboviruses represent a serious health burden in tropical and subtropical areas worldwide. Despite these burdens, antiviral therapies do not exist, and inhibitors of ZIKV are therefore urgently needed. To elucidate the anti-ZIKV effect of lycorine, we used reverse transcription-quantitative real-time PCR (qRT-PCR), immunofluorescence, Westernwestern blot, and plaque forming assay to analyse viral RNA (vRNA), viral protein, progeny virus counts, and validated inhibitors in vitro using a variety of cell lines. Additionally, we found that lycorine acts post-infection according to time-of-addition assay, and inhibits RdRp activity. Lycorine protected AG6 mice against ZIKV-induced lethality by decreasing the viral load in the blood. Due to its potency and ability to target ZIKV infection in vivo and in vitro, lycorine might offer promising therapeutic possibilities for combatting ZIKV infections in the future.

journal_name

Virology

journal_title

Virology

authors

Chen H,Lao Z,Xu J,Li Z,Long H,Li D,Lin L,Liu X,Yu L,Liu W,Li G,Wu J

doi

10.1016/j.virol.2020.04.009

subject

Has Abstract

pub_date

2020-07-01 00:00:00

pages

88-97

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(20)30075-1

journal_volume

546

pub_type

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