Abstract:
:To investigate differential evolutionary rates and selective forces of WNV in hosts and vectors, we measured the genetic diversity that arose during alternating passage in mosquitoes and birds. Within-host genetic diversity was monitored in each of three experimentally passed replicates, and the complete genome sequence of each WNV strain was determined after passage. The intrahost genetic diversity that arose during alternating passage was significantly greater than the diversity generated during chicken-only passage and similar to mosquito-only passage. dN/dS ratios suggested purifying selection similar to chick-passed virus, but not to mosquito-passed virus. Thus, the abundant genetic variation contributed to WNV populations through infection of mosquitoes and the strong purifying selection contributed by infection of birds may be maintained despite frequent host switching.
journal_name
Virologyjournal_title
Virologyauthors
Jerzak GV,Brown I,Shi PY,Kramer LD,Ebel GDdoi
10.1016/j.virol.2008.02.032subject
Has Abstractpub_date
2008-05-10 00:00:00pages
256-60issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(08)00168-2journal_volume
374pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The human papillomavirus type 8 (HPV8) is associated with skin cancer development. The goal of this study was to investigate the effects of HPV8 oncoproteins on cellular gene expression and the identification of key regulators. We performed affymetrix microarray analyses to identify differentially expressed genes and ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.06.019
更新日期:2019-09-01 00:00:00
abstract::Human rotaviruses (Wa, KUN, MO) showed hemagglutination (HA) only with fixed 1-day-old chicken erythrocytes, and their HA activities were completely destroyed by trypsin activation of virions. Simian SA-11 and bovine NCDV had HA activities not only against fixed erythrocytes but also against fresh erythrocytes from va...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90121-9
更新日期:1989-09-01 00:00:00
abstract::In a previous study on the replication of Kunjin virus using immunoelectron microscopy (E. G. Westaway, J. M. Mackenzie, M. T. Kenney, M. K. Jones, and A. A. Khromykh, 1997, J. Virol. 71, 6650-6661), NS1 and NS3 were found associated with double-stranded RNA (dsRNA) within vesicle packets (VP) in infected Vero cells, ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9156
更新日期:1998-06-05 00:00:00
abstract::CD8 T cells play an important role in controlling Ebola infection and in mediating vaccine-induced protective immunity, yet little is known about antigenic targets in Ebola that are recognized by CD8 T cells. Overlapping peptides were used to identify major histocompatibility complex class I-restricted epitopes in mic...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.09.016
更新日期:2004-01-05 00:00:00
abstract::Neutralizing monoclonal antibodies 2F1 and 1C10, which are specific for VP7 of serotype 2 rotaviruses (G2), were used to select neutralization escape variants of the human serotype 2 rotavirus, DS-1. Neutralization survival patterns generated by monoclonal antibodies 2F1, 1C10, and RV5:3 indicated that 2F1 and 1C10 di...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1601
更新日期:1993-11-01 00:00:00
abstract::The replicative form (RF) of spiroplasma virus 4 (SpV4) has been purified from infected cells of Spiroplasma melliferum strain G1 by alkaline lysis followed by low melting point agarose gel electrophoresis. A partial restriction map has been established. The circular RF was linearized by cutting at the unique ClaI res...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90060-7
更新日期:1986-06-01 00:00:00
abstract::Soluble RNA-dependent RNA polymerases from healthy and alfalfa mosaic virus (AMV)-infected leaves were purified more than 400-fold from the 100,000g supernatant of leaf homogenates, using ammonium sulfate precipitation, Sephadex G-100 filtration, and chromatography on DEAE Sephadex A25 and P11-phosphocellulose. The DE...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90554-1
更新日期:1980-01-15 00:00:00
abstract::The recent emergence and spread of dengue hemorrhagic fever in the Americas have been a major source of concern. Efforts to control this disease are dependent on understanding the pathogenicity of dengue viruses and their transmission dynamics. Pathogenicity studies have been hampered by the lack of in vitro or in viv...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8504
更新日期:1997-04-14 00:00:00
abstract::Cytoplasmic tails of envelope (Env) glycoproteins of many retroviruses inhibit their membrane fusion activity. The cytoplasmic 16-amino acid peptide of ecotropic murine leukemia virus (E-MLV) Env protein, called the R-peptide, also inhibits the membrane fusion activity of the Env protein. However, the molecular mechan...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.04.005
更新日期:2019-06-01 00:00:00
abstract::Gene transfer approaches using viruses such human adenovirus (HAdV) may provide an alternative treatment for diseases involving hematopoietic cells. Better understanding of the cellular mechanisms by which the HAdV introduces DNA into these cells should help in vector design. We examined HAdV intracellular delivery in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.09.043
更新日期:2004-03-01 00:00:00
abstract::Methods currently used for HIV-1 viral load measurements are very sensitive, but cannot distinguish between infectious and noninfectious particles. Here we describe the development of a novel, sensitive, and highly reproducible method that allows rapid isolation and quantification of infectious particles from patient ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.05.022
更新日期:2004-08-15 00:00:00
abstract::In wild-type human parainfluenza virus type 2 (WT HPIV2), one gene (the P/V gene) encodes both the polymerase-associated phosphoprotein (P) and the accessory V protein. We generated a HPIV2 virus (rHPIV2-V(ko)) in which the P/V gene encodes only the P protein to examine the role of V in replication in vivo and as a po...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.018
更新日期:2010-02-20 00:00:00
abstract::Feline calicivirus depends on host-cell proteins for its replication. We previously showed that knockdown of nucleolin (NCL), a phosphoprotein involved in ribosome biogenesis, resulted in the reduction of FCV protein synthesis and virus yield. Here, we found that NCL may not be involved in FCV binding and entry into c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.12.001
更新日期:2016-02-01 00:00:00
abstract::Significant economic losses in the poultry industries have resulted from H9N2 low pathogenic avian influenza virus infections across North Africa, the Middle East and Asia. The present study investigated the evolutionary dynamics of H9N2 viruses circulating in Korea from 1996 to 2012. Our analysis of viral population ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.11.023
更新日期:2016-01-15 00:00:00
abstract::A previous study suggested that the genomes of the arenaviruses native to North America are a product of genetic recombination between New World arenaviruses with significantly different phylogenetic histories. The purpose of this study was to extend our knowledge of the principal host relationships and evolutionary h...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.05.031
更新日期:2007-10-25 00:00:00
abstract::The tobacco etch virus 27-kDa nuclear inclusion a (NIa) proteinase was expressed in Escherichia coli as a recombinant fusion protein containing a seven-histidine tag at the amino-terminus. Catalytically active and inactive (by virtue of a single amino acid change) forms of the proteinase were purified to homogeneity i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1331
更新日期:1995-06-20 00:00:00
abstract::Proviruses were cloned directly from a cat that developed neurological disorders approximately 28 months after inoculation with a molecularly cloned, minimally pathogenic subgroup A feline leukemia virus (FeLV-A). In addition to FeLV-A proviruses that were nearly identical to the inoculated virus, we detected a subgro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1597
更新日期:1994-11-01 00:00:00
abstract::HPV16 is the most carcinogenic HPV type, but only a minority of HPV16 infections progress to cancer. Intratype genetic variants of HPV16 have been suggested to confer differential carcinogenicity. To investigate risk implications of HPV16 variants among Chinese women, a case-control study was conducted with 298 cervic...
journal_title:Virology
pub_type: 杂志文章,meta分析
doi:10.1016/j.virol.2015.11.016
更新日期:2016-01-15 00:00:00
abstract::The efficacy of combining immunization with human immunodeficiency vitus type 1 (HIV-1) DNA and HIV-1 recombinant proteins to obtain protection from chimeric simian/human immunodeficiency virus (SHIV) was determined. Four cynomolgus monkeys received four gene-gun immunizations intraepidermally of plasmid DNA encoding ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9379
更新日期:1998-10-25 00:00:00
abstract::Human papillomavirus type 16 E7 protein, expressed in Escherichia coli as fusion protein lac alpha peptide-E7 (lac-E7), was purified by electrophoresis and used to immunize rabbits to raise antisera. The anti-lac-E7 sera recognized another bacterially expressed fusion protein trpE-E7 by the Western blot method. The an...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90386-3
更新日期:1989-05-01 00:00:00
abstract::To better understand the role of specific residues within the duck hepatitis B virus (DHBV) pre-S protein in neutralization and infectivity, we have selected and identified pre-S variants which escape neutralization. A highly neutralizing monoclonal antibody (Mab 900) which recognizes an epitope 83IPQPQWTP90 localized...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8665
更新日期:1997-08-04 00:00:00
abstract::nsP3 is one of four viral nonstructural proteins required for RNA replication of Sindbis virus. In this report, post-translational modifications of nsP3 which occur in both vertebrate and mosquito cell cultures have been examined. In pulse-chase experiments, analyzed by immunoprecipitation and sodium dodecyl sulfate-p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90310-n
更新日期:1990-11-01 00:00:00
abstract::Proinflammatory cytokines and chemokines are often detected in brain tissue of patients with neurological diseases such as multiple sclerosis (MS), HIV-associated dementia (HAD) and Alzheimer's disease (AD). We have utilized a mouse model of retrovirus-induced neurological disease to examine how these proinflammatory ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.06.016
更新日期:2006-10-10 00:00:00
abstract::Two revertants of ts110 Moloney murine sarcoma virus (MuSV) with wild-type MuSV phenotype were examined for the presence of mos gene products, ts110 MuSV has a temperature-sensitive defect in a function required to maintain the transformed phenotype. The nonproducer 6m2 cell clone transformed by ts110 produces an 85,0...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90483-x
更新日期:1983-04-15 00:00:00
abstract::Priming of the viral glycoprotein (GP) by the cellular proteases cathepsin B and L (CatB, CatL) is believed to be essential for cell entry of filoviruses. However, pseudotyping systems that predominantly produce non-filamentous particles have frequently been used to prove this concept. Here, we report that GP-mediated...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.03.020
更新日期:2019-06-01 00:00:00
abstract::Bombyx mori nucleopolyhedrovirus (BmNPV) orf5 (Bm5) is a core gene of lepidopteran baculoviruses and encodes the protein with the conserved amino acid residues (DUF3627) in its C-terminus. Here, we found that Bm5 disruption resulted in lower titers of budded viruses and fewer numbers of occlusion bodies (OBs) in B. mo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.08.032
更新日期:2016-11-01 00:00:00
abstract::Epstein-Barr virus (EBV) is a herpesvirus that transforms B-cells (B-LCL) and has undergone intense scrutiny owing to its association with Burkitt's lymphoma, nasopharyngeal carcinoma, and immunoblastic lymphomas. B-LCL have also proven useful in the study of human immunology. We describe a novel system for inducing e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1069
更新日期:1994-02-01 00:00:00
abstract::L83V-related variants of human papillomavirus (HPV) 16 E6, exemplified by the Asian-American variant Q14H/H78Y/L83V, were shown to be more prevalent than E6 prototype in progressing lesions and cervical cancer. We evaluated functions relevant to carcinogenesis for the E6 variants L83V, R10/L83V and Q14H/H78Y/L83V as w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.09.036
更新日期:2009-01-05 00:00:00
abstract::Varicella zoster virus (VZV) is a neurotropic alphaherpesvirus that, following primary infection (varicella), establishes latency in sensory, autonomic, sympathetic and parasympathetic neurons, where it remains until reactivation (zoster). VZV-specific cell-mediated immune responses maintain VZV latency; thus, immunos...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.06.013
更新日期:2018-09-01 00:00:00
abstract::Although herpesviruses have a wide host range and their genomes vary substantially in size, the nucleocapsid appears to be a conservative element of viral design. The capsid shell is icosahedrally symmetric (T = 16), and 125 nm in diameter and 15nm thick in the case of herpes simplex virus 1 (HSV-1). Channel catfish v...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0016
更新日期:1996-01-15 00:00:00