Abstract:
:Entry determinants in the XPR1 receptor for the xenotropic/polytropic mouse leukemia viruses (XP-MLVs) lie in its third and fourth putative extracellular loops (ECLs). The critical ECL3 receptor determinant overlies a splice donor and is evolutionarily conserved in vertebrate XPR1 genes; 2 of the 3 rare replacement mutations at this site destroy this receptor determinant. The 13 residue ECL4 is hypervariable, and replacement mutations carrying an intact ECL3 site alter but do not abolish receptor activity, including replacement of the entire loop with that of a jellyfish (Cnidaria) XPR1. Because ECL4 deletions are found in all X-MLV-infected Mus subspecies, we deleted each ECL4 residue to determine if deletion-associated restriction is residue-specific or is effected by loop size. All deletions influence receptor function, although different deletions affect different XP-MLVs. Thus, receptor usage of a constrained splice site and a loop that tolerates mutations severely limits the likelihood of host escape mutations.
journal_name
Virologyjournal_title
Virologyauthors
Lu X,Martin C,Bouchard C,Kozak CAdoi
10.1016/j.virol.2014.07.049subject
Has Abstractpub_date
2014-11-01 00:00:00pages
63-71eissn
0042-6822issn
1096-0341pii
S0042-6822(14)00366-3journal_volume
468-470pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::In a previous paper (G. Winkler, V. B. Randolph, G. R. Cleaves, T. E. Ryan, and V. Stollar, 1988, Virology 162, 187-196) we showed that the newly synthesized dengue-2 virus nonstructural protein, NS1, exists briefly as a monomer and then undergoes dimerization. We demonstrate here that the dimerization of NS1 is assoc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90544-8
更新日期:1989-07-01 00:00:00
abstract::The terminal sequences of both genomic dsRNA segments of different strains of infectious bursal disease virus were determined. Analysis of the linear sequences revealed in the 5'-noncoding region of both segments the presence of a 32-nucleotide sequence with motifs conserved between segments and serotypes. The 3'-nonc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1226
更新日期:1995-05-10 00:00:00
abstract::Transcription of the paramyxovirus RNA genome is thought to involve a sequential stop-start mechanism whereby monocistronic mRNAs are produced by polyadenylation and termination of 3' upstream gene followed by reinitiation at the downstream start site. For a number of paramyxoviruses, transcription across the M-F gene...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9266
更新日期:1998-08-01 00:00:00
abstract::The quasispecies structure of nervous necrosis virus (NNV) was determined to investigate an outbreak of viral nervous necrosis disease at a barramundi (Lates calcarifer) hatchery. A traditional epidemiological investigation indicated horizontal transmission of infection between two cohorts of fish. However, variation ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.10.011
更新日期:2013-02-05 00:00:00
abstract::Previously, we showed that the transmembrane (M) and surface (S) glycoproteins were recognized by splenic CD4 T lymphocytes harvested from mice infected intraperitoneally with mouse hepatitis virus, strain JHM (MHV-JHM), whereas only the S protein was recognized by splenocytes derived from mice with MHV-induced chroni...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8819
更新日期:1997-11-10 00:00:00
abstract::Retrons are unusual, reverse transcriptase-encoding elements found in bacteria. Although there are a number of indications that retrons are mobile elements, their transposition has not been observed. The Escherichia coli retrons Ec67 and Ec86 are different retrons inserted at the same site and we have further characte...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0228
更新日期:1996-05-01 00:00:00
abstract::Human parvovirus B19 (B19) infects human erythroid lineage cells. Accumulating evidence also shows that B19 is detectable in nonerythroid lineage cells in vivo, but the mechanism of infection is still not clear. In this study, we explored the mode of B19 infection of human monocytic cell line U937. An in vitro infecti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.09.040
更新日期:2006-02-05 00:00:00
abstract::To study the process of membrane fusion in Morbilliviruses, the fusion (F) glycoproteins of Peste des petits ruminants virus (PPRV) and Rinderpest virus (RPV) were expressed transiently in mammalian cells. The recombinant F proteins were found to be localized at the surface of transfected cells. The fusion activity, a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1120
更新日期:2001-10-10 00:00:00
abstract::Cytoplasmic poly(A)-containing RNA from parainfluenza virus 3-infected cells was used as template to construct a cDNA library that was cloned into the EcoRV site of the plasmid pMG5. The resulting clones were screened with [32P]-labeled cDNA probes made from infected and mock-infected cell mRNAs. The virus specificity...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90237-5
更新日期:1985-11-01 00:00:00
abstract::RSV is a major cause of severe lower respiratory infection in infants and young children. With no vaccine yet available, it is important to clarify mechanisms of disease pathogenesis. Since indoleamine-2,3-dioxygenase (IDO) is an immunomodulatory enzyme and is upregulated with RSV infection, we studied it in vivo duri...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.09.007
更新日期:2017-12-01 00:00:00
abstract::Hepatitis C virus (HCV) core protein constitutes a viral nucleocapsid and may possess multiple functions. In this study, we demonstrated the homotypic interaction and multimerization of HCV core protein in vitro and in vivo. By using a yeast two-hybrid system, we showed that the amino-terminal hydrophilic portion (ami...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0164
更新日期:1996-04-01 00:00:00
abstract::Rice tungro disease is caused by a complex of two viruses, Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV). To examine the RNAi-based defence response in rice during tungro disease, we characterized the virus-derived small RNAs and miRNAs by Deep Sequencing. We found that, while 21 nt/22 nt...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.07.022
更新日期:2018-10-01 00:00:00
abstract::Our previous studies have demonstrated that virus-specific cell-mediated cytotoxicity of sensitized leukocytes can be induced using clonal ginbuna crucian carp and their syngeneic cell lines. In the present study, we attempt to determine if virus-specific cytotoxic cell populations of fish express CD8alpha and TCRbeta...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.01.019
更新日期:2006-05-10 00:00:00
abstract::Replication of the satellite RNA of tobacco ringspot virus (sTobRV RNA) has been postulated to require rolling circle transcription. The expected product of rolling circle transcription, multimeric sTobRV RNA, is known to undergo self-cleavage in vitro to release unit-length sTobRV RNA. A spontaneous, efficient, not e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90005-v
更新日期:1991-01-01 00:00:00
abstract::In order to determine the location and biological significance of the acid-induced conformational change in influenza virus hemagglutinin (HA) reported by Skehel et al., monoclonal antibodies were prepared to the molecule before and after treatment at pH 5.0. These antibodies together with monoclonal antibodies to the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(83)80015-4
更新日期:1983-04-30 00:00:00
abstract::As a simian species, the langurs are not known to harbor simian retroviruses, except for one report on a simian Type D endogenous retrovirus from the spectacled langur (Trachypithecus obscurus) from Malaysia. The present report describes for the first time natural infection of the common Hanuman langur (Semnopithecus ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0567
更新日期:2000-11-10 00:00:00
abstract::The pathogenesis of Venezuelan equine encephalitis virus (VEE) was examined in the mouse model using V3000, a virus derived from a molecular clone of the Trinidad donkey strain of VEE. These results were compared in parallel experiments with avirulent mutants of VEE derived by site-directed mutagenesis of the clone. A...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1022
更新日期:1995-02-01 00:00:00
abstract::Retroperitoneal fibromatosis herpesvirus (RFHV), the macaque homolog of the human rhadinovirus, Kaposi's sarcoma-associated herpesvirus (KSHV), was first identified in retroperitoneal fibromatosis (RF) tumor lesions of macaques with simian AIDS. We cloned and sequenced the ORF73 latency-associated nuclear antigen (LAN...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.06.022
更新日期:2006-10-10 00:00:00
abstract::The reactivates of human sera with uninduced and phorbol ester (TPA)-induced human herpesvirus-8 (HHV-8)-infected BCBL-1 cells were examined by immunofluorescence assay (IFA) and by radioimmunoprecipitation reactions (RIP). The seroprevalence of HHV-8 infections is low in the United States general population and only ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9055
更新日期:1998-03-30 00:00:00
abstract::Monoclonal antibodies (MAbs) to a recombinant form of the envelope glycoprotein gp120 of human immunodeficiency virus type 1 (HIV-1 IIIB) were raised in rats and screened for their ability to block recombinant gp120 binding to recombinant, soluble CD4 (sCD4) in vitro. Four such MAbs were identified and characterised. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90755-z
更新日期:1991-11-01 00:00:00
abstract::To determine if West Nile virus (WNV) infection of insect cells induces a protective RNAi response, Drosophila melanogaster S2 and Aedes albopictus C6/36 cells were infected with WNV, and the production of WNV-homologous small RNAs was assayed as an indicator of RNAi induction. A distinct population of approximately 2...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.04.021
更新日期:2008-07-20 00:00:00
abstract::We have developed a sensitive assay for DNA replication in mammalian cells that enables us to detect replicated DNA fragments of human cytomegalovirus. A 1/100 portion of DNA extracted from 1000 microinjected cells was subjected to polymerase chain reaction after digestion with appropriate restriction enzymes to diffe...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1038
更新日期:1993-01-01 00:00:00
abstract::Avian infectious laryngotracheitis virus (ILTV), a herpesvirus, is a highly contagious pathogen that causes an upper respiratory tract infection in chickens. It is one of the major problems in the poultry industry worldwide. Current vaccines are not satisfactory due to the induction of latent infection. Here we descri...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1399
更新日期:1994-08-01 00:00:00
abstract::Fixed rabies virus strains (ERA and CVS) produce a fatal paralytic disease in mice after intracerebral or intramuscular injection. Some antigenic mutants of both CVS and ERA viruses with a substitution in position 333 of the glycoprotein (arginine is replaced either by a glutamine, a glycine, or an isoleucine) are tot...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90122-0
更新日期:1989-09-01 00:00:00
abstract::Viruses that establish latent infection must maintain their DNA in the host nucleus through many cellular generations. Here we identify a novel mechanism by which the gammaherpesvirus Kaposi's sarcoma-associated herpesvirus (KSHV) may achieve this persistence in latently infected body cavity-based lymphoma (BCBL) cell...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9999
更新日期:1999-11-25 00:00:00
abstract::In order to investigate the global and dynamic host microRNAs (miRNAs)/messenger RNAs (mRNAs) expression alteration during in vitro acute HCV infection, a comprehensive microarray analysis was performed using human hepatoma cells. Totally, 108 human miRNAs and 1247 mRNAs were identified whose expression levels changed...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.036
更新日期:2010-03-01 00:00:00
abstract::We have utilized glycan microarray technology to determine the receptor binding properties of early isolates from the recent 2009 H1N1 human pandemic (pdmH1N1), and compared them to North American swine influenza isolates from the same year, as well as past seasonal H1N1 human isolates. We showed that the pdmH1N1 stra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.01.027
更新日期:2011-05-10 00:00:00
abstract::An immunological relationship was detected between the coat protein of the common (U1) strain of tobacco mosaic virus (TMV) and the large subunit of the ubiquitous CO2-fixing host enzyme, ribulose-1,5-biphosphate carboxylase (RuBisCo). When assayed by Western immunoblotting or indirect ELISA, polyclonal antisera to TM...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90186-8
更新日期:1986-11-01 00:00:00
abstract::The effect of rev (art/trs) gene on the level of HIV-1 envelope (env) expression using recombinant adenovirus was investigated. Recombinant adenoviruses expressing either the envelope or the rev gene of the human immunodeficiency virus type 1 (HIV-1) were constructed by inserting the gene into an expression cassette. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90438-w
更新日期:1990-04-01 00:00:00
abstract::Lassa virus RNA isolated from purified virus particles was polyadenylated and reverse transcripts were cloned into the PstI site of plasmid pUC9. Clones containing sequences of the smaller (S) segment of the Lassa virus genome were identified by hybridization with purified S RNA. They were characterized by their abili...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90278-8
更新日期:1985-07-30 00:00:00