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Autographa californica M nucleopolyhedrovirus ProV-CATH is activated during infected cell death.

Abstract：

:V-CATH, a cathepsin L-like cysteine protease encoded by the baculovirus Autographa californica M nucleopolyhedrovirus, has been shown to play an essential role in host liquefaction. Similar to cellular cathepsin L, V-CATH is synthesized as an inactive proenzyme and is activated by cleavage of the propeptide. Previous studies indicated that removal of the propeptide was rapid, occurring as soon as the protein could be detected by Western blot, 22 h postinfection. We found, however, that these results reflected artifactual processing of the proenzyme. When the protease inhibitor E-64 was used to prevent this aberration, we found that proV-CATH accumulated in infected cells and activation did not begin until the onset of cell death, at approximately 80 h postinfection. Western blot analysis of fractions of live and dead cells isolated by fluorescence-activated cell sorting revealed that mature V-CATH was found only in dead cells. The regulation of activation of proV-CATH, therefore, was quite different from that of cellular cathepsins. Acridine orange staining revealed that lysosome integrity was lost in dead cells, an occurrence that could lead to the activation of proV-CATH by lysosomal proteases.

journal_name

Virology

journal_title

Virology

authors

Hom LG,Ohkawa T,Trudeau D,Volkman LE

doi

10.1006/viro.2002.1378

subject

Has Abstract

pub_date

2002-05-10 00:00:00

pages

212-8

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042682202913784

journal_volume

296

pub_type

杂志文章

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