Abstract:
:The DNA polymerase (DNApol) and major capsid protein (MCP) genes were used as models to study promoter activity in Chilo iridescent virus (CIV). Infection of Bombyx mori SPC-BM-36 cells in the presence of inhibitors of DNA or protein synthesis showed that DNApol, as well as helicase, is an immediate-early gene and confirmed that the major capsid protein (MCP) is a late gene. Transcription of DNApol initiated 35 nt upstream and that of MCP 14 nt upstream of the translational start site. In a luciferase reporter gene assay both promoters were active only when cells were infected with CIV. For DNApol sequences between position -27 and -6, relative to the transcriptional start site, were essential for promoter activity. Furthermore, mutation of a G within the sequence TTGTTTT located just upstream of the DNApol transcription initiation site reduced the promoter activity by 25%. Sequences crucial for MCP promoter activity are located between positions -53 and -29.
journal_name
Virologyjournal_title
Virologyauthors
Nalçacioğlu R,Marks H,Vlak JM,Demirbaĝ Z,van Oers MMdoi
10.1016/j.virol.2003.08.007subject
Has Abstractpub_date
2003-12-20 00:00:00pages
321-9issue
2eissn
0042-6822issn
1096-0341pii
S0042682203006172journal_volume
317pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The natural host of SIVrcm is the red-capped mangabey (Cercocebus torquatus torquatus). Although this virus infects macaques and human PBMCs, its pathogenic potential is unknown. We serially passaged SIVrcm through 9 rhesus macaques to assess its potential for virulence. SIVrcm infected all macaques with peak viremia ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.012
更新日期:2008-09-15 00:00:00
abstract::The P670 promoter of HPV16 directs transcription of the virus late genes in the differentiating epithelium. We found that CCAAT/enhancer binding protein beta (C/EBPbeta), a key transcription factor that induces the terminal differentiation of keratinocytes, enhanced the P670-driven transcription in transient reporter ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.10.025
更新日期:2006-03-01 00:00:00
abstract::Most adenovirus transducing vectors have the cytomegalovirus major immediate-early (CMV) or the Rous sarcoma virus long terminal repeat (RSV) promoter driving expression of the transgene. Both of these promoters are highly active in transfection and transduction assays in 293 cells, in which transducing vectors are co...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1211
更新日期:2001-12-05 00:00:00
abstract::The measles virus (MV) phosphoprotein (P) and V proteins block the interferon (IFN) response by impeding phosphorylation of the signal transducer and activator of transcription 1 (STAT1) by the Janus kinase 1 (JAK1). We characterized how STAT1 mutants interact with P and JAK1 phosphorylation. Certain mutants of the li...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.06.019
更新日期:2013-09-01 00:00:00
abstract::Mixed infections of tobacco protoplasts with Cowpea chlorotic mottle virus (CCMV) and brome mosaic virus (BMV) have been studied. Fluorescent antibody staining showed that capsids of both viruses were made in doubly infected protoplasts but only infectious BMV could be recovered, i.e., BMV dominated in mixed infection...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90050-1
更新日期:1980-09-01 00:00:00
abstract::Tobacco mosaic virus (TMV) is widespread in China and causes considerable economic losses to tobacco production. The molecular epidemiology of this virus is, however, poorly understood. In this study, we sequenced the genomes of 51 TMV isolates from five tobacco-producing regions in China and investigated the dispersa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.12.001
更新日期:2019-02-01 00:00:00
abstract::Binding of a specific peptide(s) from a viral protein to major histocompatibility complex (MHC) class I molecules is a critical step in the activation of CD8(+) cytotoxic T lymphocytes (CTLs). Once activated, CTLs can cause lethal disease in an infected host, for example, by killing virus-containing ependymal and vent...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9593
更新日期:1999-04-10 00:00:00
abstract::The nucleotide and deduced amino acid sequence of the gene 4 of murine rotavirus strain Eb were determined. The gene is 2359 nucleotides in length and encodes for a protein of 775 amino acids. Comparison of the VP4 amino acid sequence of the Eb strain with several human and animal rotavirus strains which represent all...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1153
更新日期:1994-03-01 00:00:00
abstract::The second smallest subgenomic messenger RNA, mRNA5, of the coronavirus infectious bronchitis virus includes in its "5' unique region" two separate open reading frames (5a and 5b), whose coding function has not so far been established, and thus it may represent a dicistronic messenger RNA. We report here that two poly...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90094-6
更新日期:1992-01-01 00:00:00
abstract::The N-terminal domains of the NS1 protein of influenza B virus (NS1B protein) and the NS1 protein of influenza A virus (NS1A protein) share one function: binding double-stranded RNA (dsRNA). Here we show that the N-terminal domain of the NS1B protein possesses an additional function that is not shared by its NS1A coun...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1663
更新日期:2002-12-20 00:00:00
abstract::It has previously been shown that the genome of RF virus, a variant of the human papovavirus, BK, consists of two DNA species, one (R1a) with a deletion corresponding to the early and the other (R2) with a deletion corresponding to the late region of BKV (A. Pater, M.M. Pater, and G. di Mayorca (1980). J. Virol. 36, 4...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90509-3
更新日期:1983-12-01 00:00:00
abstract::Tandemers 2MG, 2MG3, 3MG and 4MG are derivatives of the potent anti-HIV-1 microbicide candidate griffithsin (GRFT). We compared these compounds anti-HIV-1 activity to GRFT using the viruses CAP206.08 and CAAN5342.A2 that have decreased sensitivity to this lectin. The 2MG and 2MG3 tandemers had similar activity to GRFT...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.01.017
更新日期:2020-05-01 00:00:00
abstract::Rabbit and human papillomaviruses are strictly epitheliotropic and their DNA replicates extrachromosomally in benign lesions (warts) of their natural host. Their tissue and host specificity is thought to be genetically controlled and may account for the inefficiency in transforming heterologous cells. In our hands, CR...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90149-8
更新日期:1987-12-01 00:00:00
abstract::The pr55gag polyprotein of HIV-1 plays a critical role in the formation of immature virus particles in the cell and during the budding process. We investigated the influence of amino acid substitutions in the p24CA- region of the gag polyprotein on the viral assembly process. Deletion of the amino acids 341-352 in the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1210
更新日期:1993-04-01 00:00:00
abstract::Covalently closed circular dimers of phage lambda DNA have been found in Escherichia coli infected with lambda. These dimers can be formed by either the lambda Red or Int systems, by a nonrecombinational replicative mechanism requiring the activity of the lambda O and P genes or by joining of the cohesive ends. Dimers...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(77)90136-2
更新日期:1977-09-01 00:00:00
abstract::We have constructed the recombinant baculovirus which expresses the human immunodeficiency virus type 1 negative factor (nef) gene. Spodoptera frugiperda cells infected with the recombinant virus produced a 27-kDa protein which reacted with rabbit antisera raised against a carboxy-terminal synthetic peptide of the Nef...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90428-e
更新日期:1991-10-01 00:00:00
abstract::The influenza RNA polymerase is known to be important in pathogenicity and adaptation of avian influenza viruses to mammalian hosts. However, the molecular mechanisms responsible are only partly understood. Here we investigated the role of the polymerase in two different, closely related, H5N1 influenza viruses - a hi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.01.036
更新日期:2010-05-25 00:00:00
abstract::The Epstein-Barr virus open reading frame BLLF1 encodes the major envelope glycoproteins gp350 and gp220. Fragments of the gp350/220 gene were expressed in Escherichia coli in order to define regions of the polypeptide chain reacting with human sera. The C-terminal half of the protein was sufficient for recognition by...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90207-6
更新日期:1992-11-01 00:00:00
abstract::In this report, we present the DNA sequence and transcriptional characterization of a gene (IR5) that maps within each of the inverted repeat (IR) segments of the equine herpesvirus type 1 (EHV-1) genome. The IR5 open reading frame (ORF) is located within both IR sequences (nucleotides 9932-10,642 of the IR). DNA sequ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90525-t
更新日期:1992-06-01 00:00:00
abstract::Hepatitis C virus (HCV) RNA replication requires viral nonstructural proteins as well as cellular factors. Recently, a cellular protein, synaptotagmin-binding, cytoplasmic RNA-interacting protein (SYNCRIP), also known as NSAP1, was found to bind HCV RNA and enhance HCV IRES-dependent translation. We investigate whethe...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.01.018
更新日期:2009-04-10 00:00:00
abstract::The DNAs of the Cas-Br-E MuLV-induced leukemias always contain somatically acquired mink cell focus-forming (MCF) recombinant proviruses. MCF recombinants could be involved during leukemogenesis at both preleukemic times and in late-stage tumors. Among the Cas-Br-E-induced non-T-, non-B-cell leukemias, viral integrati...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90241-g
更新日期:1992-12-01 00:00:00
abstract::It has recently been shown that RNA from the bottom component of cowpea mosaic virus is capable of self-replication in cowpea mesophyll protoplasts, providing a tool for the investigation of the contribution of each RNA strand toward viral multiplication. Evidence is presented here, using this system, to show that the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90239-1
更新日期:1980-10-15 00:00:00
abstract::Reactivation of latent herpes simplex virus 2 (HSV-2) infections can be characterized by episodic recurrent genital lesions and/or viral shedding. We hypothesize that infected (HSV-2(pos)) asymptomatic individuals have acquired T cell responses to specific HSV-2 antigen(s) that may be an important factor in controllin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.07.018
更新日期:2014-09-01 00:00:00
abstract::Most adenoviral vectors use in gene therapy protocols derive from species C. However, expression of the primary receptor (human Coxsackievirus and Adenovirus receptor, hCAR) for these AdV is variable on cancer cells. In vivo targeting of a therapeutic gene to specific cells has then become a major issue in gene therap...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.12.023
更新日期:2004-04-10 00:00:00
abstract::The spike (S) and hemagglutinin/esterase (HE) of bovine coronavirus (BCV) are the two envelope proteins that recognize the same receptor-determinant of 9-O-acetylneuraminic acid on host cells. However, the precise and relative roles of the two proteins in BCV infectivity remain elusive. To unequivocally determine thei...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1307
更新日期:2002-03-01 00:00:00
abstract::JC virus (JCV) Agnoprotein (Agno) plays critical roles in successful completion of the viral replication cycle. Understanding its regulatory roles requires a complete map of JCV-host protein interactions. Here, we report the first Agno interactome with host cellular targets utilizing "Two-Strep-Tag" affinity purificat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.10.005
更新日期:2020-01-15 00:00:00
abstract::Twelve lytic viruses (PgV) infecting the marine unicellular eukaryotic harmful algal bloom species Phaeocystis globosa were isolated from the southern North Sea in 2000-2001 and partially characterized. All PgV isolates shared common phenotypic features with other algal viruses belonging to the family Phycodnaviridae ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.07.002
更新日期:2005-10-10 00:00:00
abstract::Thirteen viable insertion mutants of vaccinia virus have been constructed. These mutants, containing coding sequences of the herpes simplex virus thymidine kinase (HSV-TK) gene, were generated by marker transfer via in vivo recombination. The mutants were identified using a replica filter plating technique by in situ ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90132-7
更新日期:1986-07-30 00:00:00
abstract::PO-1-Lu, the endogenous type D retrovirus of langurs (Trachypithecus obscurus) has previously been considered a progenitor to the prototype type D retrovirus, Mason Pfizer monkey virus (M-PMV/SRV-3), that became established in macaque monkeys (Macaca spp.) following a zoonosis. This study reevaluates this hypothesis t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00548-8
更新日期:2003-10-25 00:00:00
abstract::Replication-deficient adenovirus (Ad) vectors provide an efficient technology for direct DNA delivery to cells both in vitro and in vivo. We have inserted the measles virus nucleoprotein (N) gene under the control of the strong constitutive CMV major IE promoter into an Ad type 5 E1- vector to produce the recombinant ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1362
更新日期:1995-07-10 00:00:00