Abstract:
:Hepatitis C virus (HCV) RNA replication requires viral nonstructural proteins as well as cellular factors. Recently, a cellular protein, synaptotagmin-binding, cytoplasmic RNA-interacting protein (SYNCRIP), also known as NSAP1, was found to bind HCV RNA and enhance HCV IRES-dependent translation. We investigate whether this protein is also involved in the HCV RNA replication. We found that SYNCRIP was associated with detergent-resistant membrane fractions and colocalized with newly-synthesized HCV RNA. Knock-down of SYNCRIP by siRNA significantly decreased the amount of HCV RNA in the cells containing a subgenomic replicon or a full-length viral RNA. Lastly, an in vitro replication assay after immunodepletion of SYNCRIP showed that SYNCRIP was directly involved in HCV RNA replication. These findings indicate that SYNCRIP has dual functions, participating in both RNA replication and translation in HCV life cycle.
journal_name
Virologyjournal_title
Virologyauthors
Liu HM,Aizaki H,Choi KS,Machida K,Ou JJ,Lai MMdoi
10.1016/j.virol.2009.01.018subject
Has Abstractpub_date
2009-04-10 00:00:00pages
249-56issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(09)00031-2journal_volume
386pub_type
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