High-level expression of the measles virus nucleocapsid protein by using a replication-deficient adenovirus vector: induction of an MHC-1-restricted CTL response and protection in a murine model.


:Replication-deficient adenovirus (Ad) vectors provide an efficient technology for direct DNA delivery to cells both in vitro and in vivo. We have inserted the measles virus nucleoprotein (N) gene under the control of the strong constitutive CMV major IE promoter into an Ad type 5 E1- vector to produce the recombinant virus RAd68. Following infection of human fibroblasts with RAd68 in vitro, recombinant N protein was synthesized as a 60-kDa protein that represented up to 20% total soluble cell protein. Long filamentous structures were produced in both the nucleus and the cytoplasm that were similar in appearance to measles virus nucleocapsids. These "nucleocapsid-like" structures were readily purified by density gradient centrifugation. Murine immunization with RAd68 elicited (i) a humoral immune response to N, (ii) a major histocompatibility complex class I-restricted, antigen-specific cytotoxic T cell response, and (iii) protection against challenge with the measles virus CAM/RB strain in mice. This study demonstrates the capacity of replication-deficient Ad recombinants both to induce and to characterize cell-mediated immune responses.






Fooks AR,Schadeck E,Liebert UG,Dowsett AB,Rima BK,Steward M,Stephenson JR,Wilkinson GW




Has Abstract


1995-07-10 00:00:00
















  • The role of the PI3K-Akt signal transduction pathway in Autographa californica multiple nucleopolyhedrovirus infection of Spodoptera frugiperda cells.

    abstract::Many viruses activate the phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathway, thereby modulating diverse downstream signaling pathways associated with antiapoptosis, proliferation, cell cycling, protein synthesis and glucose metabolism, in order to augment their replication. To date, the role of the PI3K-Akt p...


    pub_type: 杂志文章


    authors: Xiao W,Yang Y,Weng Q,Lin T,Yuan M,Yang K,Pang Y

    更新日期:2009-08-15 00:00:00

  • Identification of viral membrane proteins required for cell fusion and viral dissemination that are modified during vaccinia virus persistence.

    abstract::Wild-type vaccinia virus WR strain forms non-fusogenic (F-) large plaques and is hemagglutinin positive (HA+) under normal conditions of virus infection. We have analyzed a collection of spontaneous, highly attenuated mutants of vaccinia virus isolated from persistently infected Friend erythroleukemia cells (E. Paez, ...


    pub_type: 杂志文章


    authors: Ortiz MA,Paez E

    更新日期:1994-01-01 00:00:00

  • Increased HIV-DNA load in CCR5-negative lymphocytes without viral phenotypic change.

    abstract::We have previously described a selective increase in HIV-DNA content in CCR5-negative lymphocytes from late stage HIV-infected patients. Here, we show that this increase occurred even in the absence of viral phenotypic switching from CCR5- to CXCR4-tropic. This leads us to hypothesize that early and late CCR5-tropic v...


    pub_type: 杂志文章


    authors: Pakarasang M,Wasi C,Suwanagool S,Chalermchockcharoenkit A,Auewarakul P

    更新日期:2006-04-10 00:00:00

  • The role of proximal and distal sequence variations in the presentation of an immunodominant CTL epitope encoded by the ecotropic AK7 MuLV.

    abstract::An emv-14-derived, replication-competent ecotropic murine leukemia virus [MuLV], designated AK7, was previously cloned from the AKXL-5 recombinant inbred mouse strain and partially characterized. While genetically encoding for an envelope-derived immunodominant CTL epitope [KSPWFTTL] located in the transmembrane regio...


    pub_type: 杂志文章


    authors: Kim V,Green WR

    更新日期:1997-09-29 00:00:00

  • Molecular characterisation of a novel pathogenic avipoxvirus from the Australian magpie (Gymnorhina tibicen).

    abstract::Avipoxviruses are significant pathogens infecting a wide range of wild and domestic bird species globally. Here, we describe a novel genome sequence of magpiepox virus (MPPV) isolated from an Australian magpie. In the present study, histopathologically confirmed cutaneous pox lesions were used for transmission electro...


    pub_type: 杂志文章


    authors: Sarker S,Batinovic S,Talukder S,Das S,Park F,Petrovski S,Forwood JK,Helbig KJ,Raidal SR

    更新日期:2020-01-15 00:00:00

  • Natural variants of human adenovirus type 3 provide evidence for relative genome stability across time and geographic space.

    abstract::Human adenovirus type 3 (HAdV-B3) has an apparently stable genome yet remains a major circulating and problematic respiratory pathogen. Comparisons of the prototype genome to genomes from three current field strains, including two isolated from epidemics, and a laboratory strain, yielded small-scale nucleotide variati...


    pub_type: 杂志文章


    authors: Mahadevan P,Seto J,Tibbetts C,Seto D

    更新日期:2010-02-05 00:00:00

  • Neutralizing epitope on penetration protein of vaccinia virus.

    abstract::The monoclonal antibody 2D5 neutralized vaccinia virus by preventing penetration of the virus and reacting with VP23-29K. The conformation of the VP23-29K was maintained by a disulfide bond(s), and the 2D5mAb reacted stronger with the nonreduced 23-kDa form than with the reduced 29-kDa form. We selected several escape...


    pub_type: 杂志文章


    authors: Ichihashi Y,Oie M

    更新日期:1996-06-15 00:00:00

  • Selection of mutant CHO clones resistant to murine gammaherpesvirus 68 infection.

    abstract::Murine gammaherpesvirus 68 (MHV68) is used as a model to study gammaherpesvirus pathogenesis both in tissue culture systems and in vivo. We used a gene-trapping approach to get insight into cellular factors involved in MHV68 infection. By generating a library of gene-trapped CHO cells, we were able to isolate several ...


    pub_type: 杂志文章


    authors: Jarousse N,Coscoy L

    更新日期:2008-04-10 00:00:00

  • Efficient translation of mRNAs in influenza A virus-infected cells is independent of the viral 5' untranslated region.

    abstract::We test the hypothesis that the translation machinery in cells infected by influenza A virus efficiently translates only mRNAs that possess the influenza viral 5' untranslated region (5'-UTR) by introducing mRNAs directly into the cytoplasm of infected cells. This strategy avoids effects due to the inhibition of the n...


    pub_type: 杂志文章


    authors: Cassetti MC,Noah DL,Montelione GT,Krug RM

    更新日期:2001-10-25 00:00:00

  • Varicella-zoster virus-specific gp140: a highly immunogenic and disulfide-linked structural glycoprotein.

    abstract::A 140,000-dalton disulfide-linked glycoprotein (gp140) specified by varicella-zoster virus (VZV) in infected cultured cells was identified and precipitated by two murine monoclonal antibodies (VZ-151 and VZ-158). When analyzed under reducing conditions by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, gp14...


    pub_type: 杂志文章


    authors: Grose C,Edwards DP,Weigle KA,Friedrichs WE,McGuire WL

    更新日期:1984-01-15 00:00:00

  • Double-stranded ribonucleic acid from carnation cryptic virus.

    abstract::The nucleic acid of carnation cryptic virus has been identified as double-stranded RNA on the basis of (i) susceptibility to RNase in 0.1 x SSC (SSC = 0.15 M NaCl-0.015 M sodium citrate buffer, pH 7.0) and resistance to RNase in 2 x SSC and to DNase, (ii) reaction with an antiserum containing antibodies to dsRNA, and ...


    pub_type: 杂志文章


    authors: Lisa V,Boccardo G,Milne RG

    更新日期:1981-12-01 00:00:00

  • Alphavirus production is inhibited in neurofibromin 1-deficient cells through activated RAS signalling.

    abstract::Virus-host interactions essential for alphavirus pathogenesis are poorly understood. To address this shortcoming, we coupled retrovirus insertional mutagenesis and a cell survival selection strategy to generate clonal cell lines broadly resistant to Sindbis virus (SINV) and other alphaviruses. Resistant cells had sign...


    pub_type: 杂志文章


    authors: Kolokoltsova OA,Domina AM,Kolokoltsov AA,Davey RA,Weaver SC,Watowich SJ

    更新日期:2008-07-20 00:00:00

  • Sequences within the early and late promoters of archetype JC virus restrict viral DNA replication and infectivity.

    abstract::Two forms of JC virus (JCV) have been isolated from its human host, an archetype found in kidney tissue and urine of nonimmunocompromised individuals and a rearranged type detected in lymphocytes and brain tissue of patients with and without progressive multifocal leukoencephalopathy. To investigate the hypothesis tha...


    pub_type: 杂志文章


    authors: Daniel AM,Swenson JJ,Mayreddy RP,Khalili K,Frisque RJ

    更新日期:1996-02-01 00:00:00

  • Sequence complementarity of sonchus yellow net virus RNA with RNA isolated from the polysomes of infected tobacco.

    abstract::Polyribosomal RNA from tobacco infected with sonchus yellow net virus (SYNV) contained sequences which hybridized to 125I-labeled SYNV RNA and which were complementary to 80 to 100% of the viral RNA genome. The poly(A)-containing RNA from polyribosomes was complementary to over 90% of the viral genome but the polyribo...


    pub_type: 杂志文章


    authors: Milner JJ,Jackson AO

    更新日期:1979-08-01 00:00:00

  • Identification of major antigenic domains of duck hepatitis B virus pre-S protein by peptide scanning.

    abstract::Neutralization epitopes of duck hepatitis B virus (DHBV) have been previously mapped within the N-terminal portion of the pre-S protein using monoclonal antibodies. However, the immune response of ducks to this region is not well characterized at the amino acid level. To this end, we have immunized adult Pekin ducks w...


    pub_type: 杂志文章


    authors: Chassot S,Lambert V,Kay A,Godinot C,Trepo C,Cova L

    更新日期:1994-04-01 00:00:00

  • Vesicular stomatitis virus and its defective interfering particles exhibit in vitro transcriptional and replicative competition for purified L-NS polymerase molecules.

    abstract::We have quantitated replication of the RNA genomes of vesicular stomatitis virus (VSV) and its defective interfering (DI) particles in a BHK21 cell-free system into which nucleocapsids were introduced in varying amounts and ratios, with or without addition of purified virus polymerase components. The quantitative tran...


    pub_type: 杂志文章


    authors: Giachetti C,Holland JJ

    更新日期:1989-05-01 00:00:00

  • Nucleotide sequence analysis of the respiratory syncytial virus subgroup A cold-passaged (cp) temperature sensitive (ts) cpts-248/404 live attenuated virus vaccine candidate.

    abstract::The complete nucleotide sequence of the RSV cpts-248/404 live attenuated vaccine candidate was determined from cloned cDNA and was compared to that of the RSV A2/HEK7 wild-type, cold-passaged cp-RSV, and cpts-248 virus, which constitute the series of progenitor viruses. RSV cpts-248/404 is more attenuated and more tem...


    pub_type: 杂志文章


    authors: Firestone CY,Whitehead SS,Collins PL,Murphy BR,Crowe JE Jr

    更新日期:1996-11-15 00:00:00

  • Identification of proteins encoded by avian reoviruses and evidence for post-translational modification.

    abstract::Twelve avian reovirus strain 176 proteins, 10 structural (lambda 1, lambda 2, lambda 3, mu 1, mu 2/mu 2C, sigma 1, sigma 2, sigma 3, and sigma 4), and 2 nonstructural (mu NS and sigma NS) were identified and characterized by electrophoretic analysis of purified virions and infected cell lysates. Three of the identifie...


    pub_type: 杂志文章


    authors: Ni Y,Ramig RF,Kemp MC

    更新日期:1993-03-01 00:00:00

  • Increased gelatinase B/matrix metalloproteinase 9 (MMP-9) activity in a murine model of acute coxsackievirus B4-induced pancreatitis.

    abstract::Infection of mice with coxsackievirus B4 results within days in a severe acute necrotizing pancreatitis, which resolves completely within weeks. Gelatinase B or matrix metalloproteinase 9 (MMP-9) has previously been shown to be involved in several models of pancreatitis, but its role in virus-induced pancreatitis has ...


    pub_type: 杂志文章


    authors: De Palma AM,Verbeken E,Van Aelst I,Van den Steen PE,Opdenakker G,Neyts J

    更新日期:2008-12-05 00:00:00

  • Biochemical analysis of the N-glycosylation pathway in baculovirus-infected lepidopteran insect cells.

    abstract::The baculovirus-insect cell system is used routinely for foreign glycoprotein production, but the precise nature of the N-glycosylation pathway in this system remains unclear. Some studies indicate that these cells cannot process N-linked oligosaccharides to complex forms containing outer-chain galactose and sialic ac...


    pub_type: 杂志文章


    authors: Jarvis DL,Finn EE

    更新日期:1995-10-01 00:00:00

  • Pathogenic prion protein fragment (PrP106-126) promotes human immunodeficiency virus type-1 infection in peripheral blood monocyte-derived macrophages.

    abstract::Transfusion of blood and blood products contaminated with the pathogenic form of prion protein Prp(sc), thought to be the causative agent of variant a Creutzfeldt-Jakob disease (vCJD), may result in serious consequences in recipients with a compromised immune system, for example, as seen in HIV-1 infection. In the pre...


    pub_type: 杂志文章


    authors: Bacot SM,Feldman GM,Yamada KM,Dhawan S

    更新日期:2015-02-01 00:00:00

  • Localization of multiple TMV encapsidation initiation sites on rbcL gene transcripts.

    abstract::TMV capsid protein reacts with and encapsidates many of the chloroplast DNA transcripts both in vivo and in vitro to form pseudovirions. We report on the encapsidation initiation reaction with one of the major RNA species found in in vivo formed pseudovirions, the mRNA for the chloroplast-encoded large subunit of ribu...


    pub_type: 杂志文章


    authors: Atreya CD,Siegel A

    更新日期:1989-02-01 00:00:00

  • Major product pp43 of human cytomegalovirus U(L)112-113 gene is a transcriptional coactivator with two functionally distinct domains.

    abstract::Human cytomegalovirus U(L)112-113 encodes four phosphoproteins, pp84, pp50, pp43, and pp34, with common amino-termini. A previous report by Kerry et al. (J. Virol. 70, 373-382, 1996) demonstrated that U(L)112-113 products activate U(L)54 promoter in cooperation with immediate-early (IE) proteins. In this study, we ide...


    pub_type: 杂志文章


    authors: Li J,Yamamoto T,Ohtsubo K,Shirakata M,Hirai K

    更新日期:1999-07-20 00:00:00

  • Cucumber mosaic virus mutants with altered physical properties and defective in aphid vector transmission.

    abstract::Two mutant strains of cucumber mosaic virus (CMV) were investigated with respect to virion stability and molecular determinants of aphid vector transmission. The mutant 2A1-MT-60x, derived from the mechanically passaged wild type 2A1-AT, is poorly transmissible by the aphid Aphis gossypii and not transmissible by the ...


    pub_type: 杂志文章


    authors: Ng JC,Liu S,Perry KL

    更新日期:2000-10-25 00:00:00

  • Further studies of the RNA synthesis phenotype selected during persistent infection with vesicular stomatitis virus.

    abstract::Vesicular stomatitis virus (VSV) isolated from two independently established lines of persistently infected mouse L cells expressed an altered phenotype of RNA synthesis at 37 degrees, the temperature at which the persistently infected cultures were maintained (T.K. Frey and J.S. Youngner, 1982, J. Virol. 44, 167-174)...


    pub_type: 杂志文章


    authors: Frey TK,Youngner JS

    更新日期:1984-07-15 00:00:00

  • Contribution of SUMO-interacting motifs and SUMOylation to the antiretroviral properties of TRIM5α.

    abstract::Recent findings suggested that the SUMO-interacting motifs (SIMs) present in the human TRIM5α (TRIM5α(hu)) protein play an important role in the ability of TRIM5α(hu) to restrict N-MLV. Here we explored the role of SIMs in the ability of rhesus TRIM5α (TRIM5α(rh)) to restrict HIV-1, and found that TRIM5α(rh) SIM mutan...


    pub_type: 杂志文章


    authors: Brandariz-Nuñez A,Roa A,Valle-Casuso JC,Biris N,Ivanov D,Diaz-Griffero F

    更新日期:2013-01-20 00:00:00

  • Functionality of host proteins in Cucumber mosaic virus replication: GAPDH is obligatory to promote interaction between replication-associated proteins.

    abstract::Here, we evaluated the role of two host proteins, a Bromo domain containing RNA binding protein (BRP1) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), in the replication of Cucumber mosaic virus (CMV). LC-MS/MS analysis of host/viral proteins pull down against BRP1 from CMV-infected plants co-infiltrated with BR...


    pub_type: 杂志文章


    authors: Chaturvedi S,Seo JK,Rao AL

    更新日期:2016-07-01 00:00:00

  • Noninfectious L-particles supply functions which can facilitate infection by HSV-1.

    abstract::The tegument of herpes simplex virus contains proteins important for the efficient initiation of infection. L-particles are noninfectious virion-related particles that lack the nucleocapsid but do contain tegument and envelope. Their ability to deliver functional proteins into cells was compared to that of virions by ...


    pub_type: 杂志文章


    authors: McLauchlan J,Addison C,Craigie MC,Rixon FJ

    更新日期:1992-10-01 00:00:00

  • Intracellular factors, but not virus receptor levels, influence the age-related outcome of DHBV infection of ducks.

    abstract::Previous serological studies of experimental infection with duck hepatitis B virus (DHBV) have shown that the outcome of infection depends largely on the age of the duck at the time of inoculation. To examine the hypothesis that decreased susceptibility with increased age might be due to the loss of the virus receptor...


    pub_type: 杂志文章


    authors: Qiao M,Gowans EJ,Burrell CJ

    更新日期:1992-02-01 00:00:00

  • Characterization of antigenic determinants in the core antigen of the hepatitis C virus.

    abstract::Antibodies to the hepatitis C virus (HCV) core protein are present in the majority of patients with chronic HCV infection. To characterize the corresponding determinants, synthetic peptides and various deletion clones of the core gene expressed in Escherichia coli were used to test human anti-core positive sera or rab...


    pub_type: 杂志文章


    authors: Goeser T,Müller HM,Ye J,Pfaff E,Theilmann L

    更新日期:1994-12-01 00:00:00