Abstract:
:We previously reported that a mutant of Mason-Pfizer monkey virus (M-PMV), which has an amino acid substitution in the matrix (MA) protein at position 55, MA-R55W, showed altered viral morphogenesis, reduced glycoprotein incorporation, and loss of infectivity. In this report, we show that two additional amino acid substitutions at this site in MA, R55F and R55Y, also result in similar altered morphogenesis, Env incorporation, and infectivity, demonstrating that these changes are not specific for the substitution of tryptophan in place of arginine 55. Attempts to isolate second site infectious revertants from cells transfected with the R55W mutant genome resulted only in the recovery of infectious viruses in which the codon at position 55 had reverted to one encoding arginine. In contrast, no revertants were obtained from the phenylalanine and tyrosine mutants in which three nucleotide changes had been engineered into the arginine codon. These results confirm that the arginine residue at position 55 is critical for intracellular targeting of M-PMV Gag molecules and support the concept that as part of a cytoplasmic transport retention signal R55 interacts with cellular trafficking components rather than other regions of Gag.
journal_name
Virologyjournal_title
Virologyauthors
Yasuda J,Hunter Edoi
10.1006/viro.1999.0179subject
Has Abstractpub_date
2000-03-15 00:00:00pages
533-8issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(99)90179-4journal_volume
268pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::To identify ranavirus virulence genes, we engineered Frog Virus 3 (FV3) knockout (KO) mutants defective for a putative viral caspase activation and recruitment domain-containing (CARD) protein (Δ64R-FV3) and a β-hydroxysteroid dehydrogenase homolog (Δ52L-FV3). Compared to wild type (WT) FV3, infection of Xenopus tadpo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.07.011
更新日期:2015-11-01 00:00:00
abstract::Inbred mice bearing the FV-1n marker when inoculated at birth with an ecotropic murine leukemia virus (WM 1504-E) obtained from wild mice develop a progressive central nervous system (CNS) disease manifested by hindlimb paralysis and incoordination that begins by 6 to 7 weeks of age. Studies of infected SWR/J mice at ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90326-4
更新日期:1983-07-15 00:00:00
abstract::Replication and transmission of avian influenza virus in humans poses a pandemic threat. The molecular determinants that facilitate this process are not well understood. We used DBA/2 mice to identify viral factors that mediate the difference in pathogenesis between a virulent (H7N3) and a non-virulent (H7N9) avian in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.04.013
更新日期:2016-07-01 00:00:00
abstract::The heptauridine tract at each gene end and intergenic region (IGR) at the gene junctions of vesicular stomatitis virus (VSV) have effects on synthesis of the downstream mRNA, independent of their respective roles in termination of the upstream mRNA. To investigate the role of the U tract and the IGR in downstream gen...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.08.001
更新日期:2008-10-25 00:00:00
abstract::Proinflammatory cytokines and chemokines are often detected in brain tissue of patients with neurological diseases such as multiple sclerosis (MS), HIV-associated dementia (HAD) and Alzheimer's disease (AD). We have utilized a mouse model of retrovirus-induced neurological disease to examine how these proinflammatory ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.06.016
更新日期:2006-10-10 00:00:00
abstract::Mice inoculated with replication-competent stocks of the murine acquired immunodeficiency syndrome (MAIDS) virus are severely immunocompromised and proned to the development of T- and B-cell lymphomas. We have studied the development of T-cell lymphomas in C57BL/6 and RF/J mice inoculated with helper-free stocks of th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80072-7
更新日期:1995-01-10 00:00:00
abstract::We used serial protein misfolding cyclic amplification (sPMCA) to amplify the D10 strain of CWD prions in a linear relationship over two logs of D10 dilutions. The resultant PMCA-amplified D10 induced terminal TSE disease in CWD-susceptible Tg(cerPrP)1536 mice with a survival time approximately 80 days shorter than th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.09.023
更新日期:2008-12-20 00:00:00
abstract::The requirement for the herpes simplex virus type 1 (HSV-1) protein Vmw65 (VP16) for activation of immediate early (IE) gene expression was examined in synchronized HeLa cells. Analyses of IE RNA levels were conducted during infection with a viral Vmw65 mutant, in1814. The results revealed an increased requirement for...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90695-l
更新日期:1992-07-01 00:00:00
abstract::The induction of autoimmunity by viruses has been hypothesized to occur by a number of mechanisms. Coxsackievirus B4 (CB4) induces hyperglycemia in SJL mice resembling diabetes in humans. While virus is effectively cleared within 2 weeks, hyperglycemia does not appear until about 8-12 weeks postinfection at a time whe...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00462-8
更新日期:2003-09-30 00:00:00
abstract::The poxvirus virion contains an inner tubular nucleocapsid structure. The nucleocapsid is apparently labile to conventional electron microscopy fixation procedures and has therefore been largely ignored for decades. Advancements in electron microscopy sample preparation, notably high pressure freezing, better preserve...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.08.019
更新日期:2012-12-05 00:00:00
abstract::Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac92 is a core gene encoding a protein associated with occlusion derived virus (ODV), binds human P53 and also has flavin adenine dinucleotide linked sulfhydryl oxidase activity but its role in the virus life cycle is not known. To determine ac92 function a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.09.023
更新日期:2011-01-05 00:00:00
abstract::The cellular protein MDM2 can bind to the tumor suppressor gene product p53 and abrogate its transcriptional activity. In addition, p53 can regulate expression of the mdm2 gene. We and others have previously shown that p53 is present at high levels in adenovirus-transformed cells which express the larger E1B protein. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1349
更新日期:1995-07-10 00:00:00
abstract::Poliovirus readily establishes a persistent infection in the K562-Mu erythroleukemia cell strain. In this study, three additional K562 cell strains were analyzed for their responses to poliovirus infection and found to be quite variable. K562 cells obtained from the ATCC established a persistent infection, similar to ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1541
更新日期:1995-10-20 00:00:00
abstract::Specific binding sites for Epstein-Barr virus (EBV) nuclear antigen (EBNA-1), isolated and semipurified from EBV-transformed nonproductive Raji cells, were visualized on the molecule of EBV DNA by electron microscopy and mapped. Two measures had to be applied to counteract the limited purity of the EBNA-1 preparation:...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90025-0
更新日期:1987-10-01 00:00:00
abstract::Avian myelocytomatosis virus MC29 inoculated into the wing web of 1-day-old Brown Leghorn chickens causes a high incidence of tumors, predominantly endotheliomas that are apparently induced directly by the action of the viral myc gene. Mutants of MC29, in which portions of the v-myc gene have been deleted and which ha...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90300-8
更新日期:1983-01-15 00:00:00
abstract::The complete nucleotide sequence of cloned cDNAs corresponding to the full length of the mRNA encoding the measles virus hemagglutinin (H) protein has been determined. the mRNA contains a single large open reading frame which is capable of encoding a protein of 617 amino acids with a molecular mass of 69,250 Da. The d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90312-0
更新日期:1986-04-30 00:00:00
abstract::In this study, we present enzymatic differences found between recombinant RTs derived from feline leukemia virus and feline immunodeficiency virus. Firstly, FIV RT showed low steady state K(m) values for dNTPs compared to FeLV RT. Consistent with this, FIV RT synthesized DNA more efficiently than FeLV RT at low dNTP c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.02.010
更新日期:2005-04-25 00:00:00
abstract::The monocytoid tumor cell line U-937 and five derived subclones were infected with the HTLV-IIIB isolate of the human immunodeficiency virus (HIV). Susceptibility to infection and sensitivity to the cytopathic effects correlated with the expression of the T4 antigen on the cell surface. On the basis of these character...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90278-9
更新日期:1987-04-01 00:00:00
abstract::The complete DNA sequence of a new lytic T7-like bacteriophage phiKMV is presented. It is the first genome sequence of a member of the Podoviridae that infects Pseudomonas aeruginosa. The linear G + C-rich (62.3%) double-stranded DNA genome of 42,519 bp has direct terminal repeats of 414 bp and contains 48 open readin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00123-5
更新日期:2003-07-20 00:00:00
abstract::HPV16 E6 deregulates G1/S cell cycle progression through p53 degradation preventing transcription of the CDK inhibitor p21(WAF1). However, additional mechanisms independent of p53 inactivation appear to exist. Here, we report that HPV16 E6 targets the cellular factor p150(Sal2), which positively regulates p21(WAF1) tr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.05.016
更新日期:2011-09-01 00:00:00
abstract::Previously a new small subclass of SV40 large T antigen with a high-binding affinity to living target cells was characterized (J. Lange-Mutschler and R. Henning, 1983, Virology 127, 333-344.) In the present study the external binding process, particularly the tight linkage of T antigen to lipid of the target cells, wa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90176-4
更新日期:1984-07-30 00:00:00
abstract::Feline coronavirus infection can progress to a fatal infectious peritonitis, which is a widespread feline disease without an effective vaccine. Generating feline cells with reduced ability to respond to interferon (IFN) is an essential step facilitating isolation of new candidate vaccine strains. Here, we describe the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.08.030
更新日期:2019-11-01 00:00:00
abstract::NS35, a basic protein encoded by gene 8 of SA11 rotavirus, possesses RNA-binding activity and is essential for genome replication. To identify conserved regions in the NS35 gene and its protein product, we determined the nucleotide sequences of the NS35 gene for the mammalian and avian rotaviruses Wa, DS1, SA11 (Patto...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1059
更新日期:1993-02-01 00:00:00
abstract::Human immunodeficiency virus type 1 subtype C (HIV-1C) accounts for about 50% of all HIV infections in the pandemic and is the predominant subtype in the heavily burdened region of southern Africa. HIV-1C possesses unique genetic and phenotypic features that might be associated with biological differences compared to ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.047
更新日期:2006-04-10 00:00:00
abstract::Feline immunodeficiency virus (FIV) is a lentivirus associated with an immunodeficiency syndrome of the domestic cat. A short open reading frame (ORF2), of unknown function, is present in all FIV isolates. We have investigated the role of ORF2 in determining the cell tropism of two infectious molecular clones of FIV. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0002
更新日期:1996-01-01 00:00:00
abstract::Geographical separation of host species has shaped the avian influenza A virus gene pool into independently evolving Eurasian and American lineages, although phylogenetic evidence for gene flow and reassortment indicates that these lineages also mix on occasion. While the evolutionary dynamics of the avian influenza g...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.05.002
更新日期:2009-08-01 00:00:00
abstract::Two tick-borne encephalitis (TBE) virus variants were studied: mouse brain-adapted strain EK-328 and its derivate adapted to Hyalomma marginatum ticks. The tick-adapted virus exhibited small-plaque phenotype and slower replication in PEK cells, higher yield in ticks, decreased neuroinvasiveness in mice, increased bind...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.12.013
更新日期:2007-05-25 00:00:00
abstract::The mechanism by which the large-size poxviruses enter animal cells is not known. In this investigation we show that acid pH treatment of wild-type vaccinia virus-infected cells triggers strong fusion of cells in culture, with an optimum at pH 4.8. We have identified the virus-induced fusion protein as a 14-kDa envelo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90381-z
更新日期:1990-09-01 00:00:00
abstract::We have used a yeast two-hybrid system to identify a baculoviral gene encoding a protein kinase-interacting protein (PKIP). The pkip gene is located at 15.8-16.2 m.u (map units) and represents ORF 24 in the sequence of Ayres et al. (1994) of the Autographa californica nuclear polyhedrosis virus. PKIP is a 19.2-kDa pro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8944
更新日期:1998-01-20 00:00:00
abstract::Clones have been constructed containing full-length cDNA copies of PEBV RNA1 and RNA2, flanked by the CaMV 35 S RNA promoter and the nopaline synthase terminator. The clones are infectious when inoculated onto Nicotiana benthamiana plants. Both the viral RNAs and the virus particles were identified in infected plants....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90488-b
更新日期:1992-04-01 00:00:00