Abstract:
:Plasmids containing the E1 region of Ad12 DNA can transform certain rodent cells into oncogenic cells. To study the role of the E1a subregion in the process of oncogenic transformation, Ad12 region E1 mutants carrying deletions in the E1a region were constructed. Deletion mutants pR7 and pR8 affect only the 13 S mRNA species encoded by region E1a, whereas deletion mutants pR11 and pR15 damage both the 12 S and 13 S E1a mRNA. All four mutants have lost their capacity to transform primary cultures of baby rat kidney cells, indicating that the E1a gene product encoded by 13 S mRNA is essential for transformation. It was further found that the mutated E1a regions of both pR7 and pR11 can induce expression of region E1b, which implies that the transformation deficiency of these mutants is not due to the inability to activate E1b expression. Surprisingly, the transforming capacity of pR7 and pR11 is restored when these mutant E1 regions are covalently coupled to the SV40 "enhancer" region. Cells transformed by these hybrids plasmids, however, were not tumorigenic in nude mice.
journal_name
Virologyjournal_title
Virologyauthors
Bos JL,Jochemsen AG,Bernards R,Schrier PI,van Ormondt H,van der Eb AJdoi
10.1016/0042-6822(83)90178-2subject
Has Abstractpub_date
1983-09-01 00:00:00pages
393-400issue
2eissn
0042-6822issn
1096-0341journal_volume
129pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The majority of HIV-1 infections occur via sexual intercourse. Women are the most affected by the epidemic, particularly in developing countries, due to their socio-economic dependence on men and the fact that they are often victims of gender based sexual violence. Despite significant efforts that resulted in the redu...
journal_title:Virology
pub_type: 杂志文章,评审
doi:10.1016/j.virol.2016.07.004
更新日期:2016-10-01 00:00:00
abstract::In response to oncogenic insults, normal human cells execute a defense response that culminates in cellular suicide, apoptosis. Normal human diploid fibroblasts expressing the human papillomavirus type 16 (HPV-16) E7 oncoprotein are predisposed to apoptosis when they are deprived of growth factors. Even though a domin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.11.008
更新日期:2004-02-05 00:00:00
abstract::Studies of the cellular autophagy pathway have exploded over the past twenty years. Now appreciated as a constitutive degradative mechanism that promotes cellular homeostasis, autophagy is also required for a variety of developmental processes, cellular stress responses, and immune pathways. Autophagy certainly acts a...
journal_title:Virology
pub_type: 杂志文章,评审
doi:10.1016/j.virol.2015.03.042
更新日期:2015-05-01 00:00:00
abstract::We have recently described the cloning of a portion of the hepatitis E virus (HEV) and confirmed its etiologic association with enterically transmitted (waterborne, epidemic) non-A, non-B hepatitis. The virus consists of a single-stranded, positive-sense RNA genome of approximately 7.5 kb, with a polyadenylated 3' end...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90760-9
更新日期:1991-11-01 00:00:00
abstract::A series of clones has been derived from an interferon-resistant murine cell line, Ltk- aprt-, and their antiviral properties have been characterized. In the parental Ltk- aprt- line interferon is unable to establish antiviral properties or to increase the levels of 2,5-oligo(A) synthetase, the 2,5-oligo(A)-activated ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90400-x
更新日期:1988-01-01 00:00:00
abstract::While DNA vaccines have been shown to prime cellular immune responses, levels are often low in nonhuman primates or humans. Hence, efforts have been directed toward boosting responses by combining DNA with different vaccination modalities. To this end, a polyvalent DNA prime/protein boost vaccine, consisting of codon ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.10.038
更新日期:2006-03-01 00:00:00
abstract::The growth of vesicular stomatitis virus requires two distinct RNA synthetic events: transcription of messenger RNA molecules and replication of the viral genome RNA. We report the use of a panel of monoclonal antibodies directed against the viral phosphoprotein P in an attempt to assess the role of this protein in RN...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0031
更新日期:1996-02-01 00:00:00
abstract::Homologous, subgenomic fragments of the viral LCR and E6/E7 transforming genes of HPV-18 and HPV-16 were amplified from several primary cervical, penile, and vulvar tumors and cloned into a pUC-18-derived vector. When assayed by a quantitative transformation assay using primary human keratinocytes, the subgenomic regi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90507-8
更新日期:1991-03-01 00:00:00
abstract::Dengue-2 virus strains from different locations were compared by T1-RNAse-resistant oligonucleotide fingerprinting and antigen signature analysis. The latter technique involved construction of radioimmunoassays using monoclonal antibodies that recognize nine distinct dengue-2 type-specific and flavivirus cross-reactiv...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90457-5
更新日期:1986-10-30 00:00:00
abstract::Human papillomaviruses (HPVs) are epitheliotropic viruses, and their life cycle is intimately linked to the stratification and differentiation state of the host epithelial tissues. Defining a role for the E5 gene product in the differentiation-dependent viral life cycle has been difficult due to the lack of a suitable...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9262
更新日期:1998-09-01 00:00:00
abstract::Adenovirus 40 (Ad40) is defective for growth in tissue culture but is complemented when the Ad2/5 or Ad12 E1B 55K protein is supplied in trans. Ad40 E1B mRNA has not been detected in E1-transformed cells, or at early times in lytically infected cells. In cells constitutively expressing the E1B region of Ad2, Ad40 E1B ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90478-t
更新日期:1991-03-01 00:00:00
abstract::Direct attenuation of West Nile (WN) virus strain NY99 for the purpose of vaccine development is not feasible due to its high virulence and pathogenicity. Instead, we created highly attenuated chimeric virus W1806 with the serological identity of NY99. To further attenuate W1806, we investigated effects of mutations f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.10.015
更新日期:2016-01-01 00:00:00
abstract::Traditional virus inoculation of plants involves mechanical rubbing of leaves, whereas in nature viruses like Tomato bushy stunt virus (TBSV) are often infected via the roots. A method was adapted to compare leaf versus root inoculation of Nicotiana benthamiana and tomato with transcripts of wild-type TBSV (wtTBSV), a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.01.011
更新日期:2013-05-10 00:00:00
abstract::The longest open reading frame of turnip yellow mosaic virus genomic RNA (ORF-206) encodes a 206-kDa nonstructural protein. The most prominent in vitro translation products of ORF-206 are the full-length p206 and a shorter N-coterminal 150-kDa protein. We have confirmed these assignments by immunoprecipitation of in v...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90851-2
更新日期:1991-09-01 00:00:00
abstract::Adsorption and one-step growth experiments performed with blue-green algal virus (BGAV) LPP-1 under normal conditions revealed a slow adsorption rate, in comparison with bacteriophage systems, followed by a 7-hour latent period. The burst size was approximately 100 virus particles per cell. The molecular weight of the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(67)90036-0
更新日期:1967-08-01 00:00:00
abstract::Natural infection and vaccination with a live-attenuated measles virus (MV) induce CD8(+) T-cell-mediated immune responses that may play a central role in controlling MV infection. In this study, we show that newly identified human HLA-A2 epitopes from MV hemagglutinin (H) and fusion (F) proteins induced protective im...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.04.040
更新日期:2006-09-01 00:00:00
abstract::Retroviral Gag proteins function during early and late stages of the viral life cycle. To gain additional insight into the cellular requirements for viral replication, a two-hybrid screen was used to identify cellular proteins that interact with the Mason-Pfizer monkey virus Gag protein. One of the cellular proteins f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00348-9
更新日期:2003-09-15 00:00:00
abstract::Many viruses activate the phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathway, thereby modulating diverse downstream signaling pathways associated with antiapoptosis, proliferation, cell cycling, protein synthesis and glucose metabolism, in order to augment their replication. To date, the role of the PI3K-Akt p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.06.007
更新日期:2009-08-15 00:00:00
abstract::Cytotoxic T-cell clones raised against X-31 (H3N2) influenza virus in C57BL/6 mice can be directed against an influenza A virus subtype specific determinant (1). A representative T-cell clone (A3.1) has been used in combination with a set of genetically typed recombinant viruses, to show that the A/PR/8/34 nucleoprote...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90413-6
更新日期:1984-03-01 00:00:00
abstract::The existence and genetic make-up of most primate retroviruses was revealed by studies of bushmeat and fecal samples from unhabituated primate communities. For these, detailed data on intra- and within-species contact rates are generally missing, which makes identification of factors influencing transmission a challen...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.05.012
更新日期:2014-07-01 00:00:00
abstract::SIV/Mne circle junctions were amplified by the polymerase chain reaction (PCR) and cloned in a bacterial plasmid. Sequence analysis of clones isolated from 11 independent PCRs reveals that the start site for plus DNA synthesis is 5' ACTG. . ., and thus an asymmetric cleavage must occur during viral integration. In add...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1329
更新日期:1993-06-01 00:00:00
abstract::Turnip crinkle carmovirus (TCV) has a uniquely high recombination frequency and nonrandom cross-over site distribution among the recombining TCV-associated satellite RNAs. An in vitro system has been developed that includes a partially purified TCV replicase preparation (RdRp) and chimeric RNAs that resemble the putat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9342
更新日期:1998-09-30 00:00:00
abstract::The HCMV IE2 protein promiscuously activates transcription of many viral and cellular genes. IE2 also negatively autoregulates its own expression by binding to a strategically positioned IE2 binding site, called CRS, located immediately downstream of the TATA box of the HCMV major IE promoter. Here we show that IE2 is...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8855
更新日期:1997-11-24 00:00:00
abstract::The utilization of transgenic plants expressing recombinant antigens to be used in the formulation of experimental immunogens has been recently communicated. We report here the development of transgenic plants of alfalfa expressing the structural protein VP1 of foot and mouth disease virus (FMDV). The presence of the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9590
更新日期:1999-03-15 00:00:00
abstract::Human CD4 was expressed on a range of mammalian cell lines. CD4+ non-primate cells, derived from rat, hamster, mink, cat, and rabbit, bind recombinant gp120 of human immunodeficiency virus type 1 (HIV-1) but are resistant to HIV-1 infection. CD4 expression on various human, rhesus, and African green monkey cell lines ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90904-p
更新日期:1991-04-01 00:00:00
abstract::Tetherin (ST2/CD317) is a cellular protein that restricts the release from cells of some enveloped viruses including HIV-1. To examine if influenza virus is affected by tetherin, MDCK cells constitutively expressing human tetherin and control MDCK cells were infected with influenza virus. No difference was observed in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.05.006
更新日期:2011-08-15 00:00:00
abstract::The Autographa californica nuclear polyhedrosis virus da26 gene codes for an envelope protein of both budded virus (BV) and occlusion derived virus (ODV). Western blot and temporal analysis of infected cell extracts detected a protein of 26 kDa by 4 h postinfection (p.i.). The amount of protein increased by 16 h p.i. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8903
更新日期:1998-01-05 00:00:00
abstract::Experiments with monoclonal antibodies raised against two laboratory strains of Sindbis virus, SB and SIN, suggested the existence of a strain-specific neutralizing antigenic site (E2-b) on the E2 glycoprotein. A comparison of monoclonal antibody binding patterns and E2 glycoprotein gene sequences of six laboratory st...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90175-9
更新日期:1987-11-01 00:00:00
abstract::Gene transfer into human hematopoietic stem cells using Ad5 is inefficient due to lack of the primary receptor CAR and the secondary receptors alphavbeta3 integrin and alphavbeta5 integrin, and due to the high seroprevalence of Ad5 antibodies in most adults, resulting in diminished gene transduction. In the present st...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.07.018
更新日期:2004-10-25 00:00:00
abstract::In vitro packaging experiments with phage P22 using artificially ligated plasmid concatemers have shown that the pac site is not necessary for DNA packaging although in vivo this initiation signal is indispensable. This indicates that the phage-coded protein gp3 also executes other important functions during phage mat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90273-9
更新日期:1985-07-30 00:00:00