Abstract:
:Traditional virus inoculation of plants involves mechanical rubbing of leaves, whereas in nature viruses like Tomato bushy stunt virus (TBSV) are often infected via the roots. A method was adapted to compare leaf versus root inoculation of Nicotiana benthamiana and tomato with transcripts of wild-type TBSV (wtTBSV), a capsid (Tcp) replacement construct expressing GFP (T-GFP), or mutants not expressing the silencing suppressor P19 (TBSVΔp19). In leaves, T-GFP remained restricted to the cells immediately adjacent to the site of inoculation, unless Tcp was expressed in trans from a Potato virus X vector; while T-GFP inoculation of roots gave green fluorescence in upper tissues in the absence of Tcp. Conversely, leaf inoculation with wtTBSV or TBSVΔp19 transcripts initiated systemic infections, while upon root inoculation this only occurred with wtTBSV, not with TBSVΔp19. Evidently the contribution of Tcp or P19 in establishing systemic infections depends on the point-of-entry of TBSV in the plants.
journal_name
Virologyjournal_title
Virologyauthors
Manabayeva SA,Shamekova M,Park JW,Ding XS,Nelson RS,Hsieh YC,Omarov RT,Scholthof HBdoi
10.1016/j.virol.2013.01.011subject
Has Abstractpub_date
2013-05-10 00:00:00pages
89-96issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(13)00042-1journal_volume
439pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Red sea bream iridovirus (RSIV) of the Iridoviridae family is a causative agent of lethal infections in many cultured marine fish species in southwestern Japan. RSIV-induced apoptosis was divided as follows: (1). cell shrinkage and rounding at the early apoptotic stage, (2). cell enlargement at the middle apoptotic st...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.02.006
更新日期:2004-05-01 00:00:00
abstract::Viral infections of the central nervous system and immune responses to these infections cause a variety of neurological diseases. Infection of weanling mice with Sindbis virus causes acute nonfatal encephalomyelitis followed by clearance of infectious virus, but persistence of viral RNA. Infection with a neuroadapted ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00110-7
更新日期:2003-06-20 00:00:00
abstract::It is now well recognized that mutations in the hepatitis B virus (HBV) genome occur during the natural course of chronic viral infection. Regions of the viral genome that are frequently affected by such mutations, rearrangements, and/or deletions generally involve the precore promoter, precore, and core as well as th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8594
更新日期:1997-07-07 00:00:00
abstract::The causal agent of rhizomania disease, Beet necrotic yellow vein virus (BNYVV), typically produces asymptomatic root-limited infections in sugar beets (Beta vulgaris) carrying the Rz1-allele. Unfortunately, this dominant resistance has been recently overcome. Multiple cDNA clones of the viral pathogenic determinant p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.03.008
更新日期:2008-06-20 00:00:00
abstract::Infection with bovine papillomavirus type 1 (BPV1) or BPV2 induces fibropapillomas in cows and skin sarcoids in horses. Prophylactic vaccination targeting BPV1 and BPV2 may reduce the incidence of these economically important diseases. The L1 major capsid proteins of BPV1 and BPV2 were expressed in Sf-9 insect cells a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.036
更新日期:2009-10-10 00:00:00
abstract::RNA interference (RNAi) is the process by which double-stranded RNA (dsRNA) directs sequence-specific degradation of messenger RNA in animal and plant cells. In mammalian cells, RNAi can be triggered by 21-23 nucleotide duplexes of small interfering RNA (siRNA). Strategies to inhibit RNA virus multiplication based on ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.01.051
更新日期:2005-05-25 00:00:00
abstract::Priming of the viral glycoprotein (GP) by the cellular proteases cathepsin B and L (CatB, CatL) is believed to be essential for cell entry of filoviruses. However, pseudotyping systems that predominantly produce non-filamentous particles have frequently been used to prove this concept. Here, we report that GP-mediated...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.03.020
更新日期:2019-06-01 00:00:00
abstract::The DNAs from the granulosis viruses of Plodia interpunctella and Pieris rapae were clearly distinguishable based on restriction endonuclease fragment patterns. By sizing single- and double-digestion products approximate molecular weights of 72 x 10(2) and 75 x 10(6), respectively, were estimated for the viral genomes...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90357-8
更新日期:1980-07-30 00:00:00
abstract::The eukaryotic translation initiation factor eIF4E, the cap-binding protein, seems to play an essential role in the establishment of the host shut-off after viral infection. Infection with adenovirus and influenza virus caused dephosphorylation of eIF4E and an involvement of a viral protein was suggested. In this repo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0143
更新日期:1996-03-15 00:00:00
abstract::A reporting replicon of West Nile virus (WN) was used to distinguish between the function of the 3' untranslated region (UTR) in viral translation and RNA replication. Deletions of various regions of the 3' UTR of the replicon did not significantly affect viral translation, but abolished RNA replication. A systematic ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.07.022
更新日期:2005-01-20 00:00:00
abstract::Cardioviruses cause diseases in many animals including, in rare cases, humans. Although they share common features with all picornaviruses, cardioviruses have unique properties that distinguish them from other family members, including enteroviruses. One feature shared by all picornaviruses is the covalent attachment ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.01.010
更新日期:2018-03-01 00:00:00
abstract::Antigenic sites on the G1 glycoprotein of La Crosse bunyavirus were defined by constructing a panel of neutralizing and nonneutralizing monoclonal antibodies (F. Gonzalez-Scarano, R. E. Shope, C. H. Calisher, and N. Nathanson (1982), Virology 120, 42-53). To analyze the relationship between the individual epitopes del...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90283-1
更新日期:1985-07-30 00:00:00
abstract::The V protein of Sendai virus (SeV) is nonessential for virus replication in cell culture but indispensable for viral pathogenicity in mice. At the C terminus of the V protein, there are amino acid residues conserved among the members of the Paramyxovinae subfamily that are clustered in three regions: region I, just d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1516
更新日期:2002-08-01 00:00:00
abstract::This paper reports the molecular cloning of a rearranged c-myc region from the FT-1 cell line, which was derived from a spontaneous feline T-cell leukemia carrying the feline leukemia virus (FeLV). An abnormal c-myc EcoRI fragment of about 18 kilobases, detected by Southern blotting, was molecularly cloned from the DN...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90172-4
更新日期:1989-04-01 00:00:00
abstract::Two tick-borne encephalitis (TBE) virus variants were studied: mouse brain-adapted strain EK-328 and its derivate adapted to Hyalomma marginatum ticks. The tick-adapted virus exhibited small-plaque phenotype and slower replication in PEK cells, higher yield in ticks, decreased neuroinvasiveness in mice, increased bind...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.12.013
更新日期:2007-05-25 00:00:00
abstract::Edible vaccines that can be made widely available and easily administered could bring great benefit to the worldwide battle against pandemic viral infections. They can be used not only for the vaccination of humans and domesticated animals, but also for wild herds and live stock which are otherwise difficult to vaccin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.08.007
更新日期:2010-11-25 00:00:00
abstract::Deletion of the nef gene results in viral attenuation and confers protection against challenge with wild-type simian immunodeficiency virus in macaques. Regarding HIV-1 infection, a few long-term nonprogressors (LTNP) with nef deletions have been described. In this study, the nef genes of a group of seven LTNP and eig...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9783
更新日期:1999-07-05 00:00:00
abstract::Seven envelope regions from two series of patient isolates have been molecularly cloned and analyzed for replication phenotypes and immunogenicity. Growth potential was analyzed for env sequences substituted into an HIV-1-NL4-3 backbone (NL4-3/env recombinants). Immunogenicity studies were conducted on secreted monome...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8445
更新日期:1997-03-03 00:00:00
abstract::The M128L myxoma virus gene expresses a five-membrane spanning cell surface protein with significant amino acid homology to the cellular CD47 proteins. CD47, also called integrin-associated protein (IAP), is associated with the modulation of leukocyte adhesion, motility, activation, and phagocytosis. Creation of an M1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.03.037
更新日期:2005-06-20 00:00:00
abstract::Measles virus V protein represses genome replication through a poorly understood mechanism, which led us to investigate whether V protein might be an RNA-binding modulatory factor. Recombinant V protein, expressed from transfected HEp-2 cells or E. coli, formed protein-RNA complexes with poly-guanosine (poly-G) or pol...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.12.018
更新日期:2006-04-25 00:00:00
abstract::Infection of eukaryotic cells in culture with herpes simplex virus type-1 (HSV-1) or HSV-2 increased the mutation frequency of the supF gene carried on the shuttle vector pZ189 by around sixfold. The increase was apparent 2 hr postinfection and reached a peak after 8 hr. To investigate this mutagenesis, plasmids pCKRR...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90600-g
更新日期:1991-06-01 00:00:00
abstract::West Nile virus is a mosquito-borne, neurotropic flavivirus that causes encephalitis in humans and animals. Since being introduced into the Western hemisphere in 1999, WNV has spread rapidly across North America, identifying this virus as an important emerging pathogen. In this study, we developed a DNA-launched infec...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.01.021
更新日期:2005-03-30 00:00:00
abstract::For purposes of gene therapy, the tropism of adenovirus (Ad) serotype 5 vectors can be altered with fibers derived from alternative serotypes. However, there is currently limited information available on the cellular receptors used by the approximately 51 known Ad serotypes. Recently, alpha(2-->3)-linked sialic acid (...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.04.001
更新日期:2004-06-20 00:00:00
abstract::Classical swine fever virus (CSFV) nonstructural protein NS5A is a multifunctional protein functioning in regulation of viral genome replication, protein translation and assembly by interaction with viral or host proteins. Here, heat shock protein 27 (Hsp27) has been identified as a novel binding partner of NS5A by us...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.02.020
更新日期:2018-05-01 00:00:00
abstract::A non-optimal receptor-binding specificity of avian influenza viruses is believed to hamper their replication in humans; however, the magnitude of this restriction remains undefined. Here we generated recombinant viruses, R1 and R2, that differed solely by two amino acids in the receptor-binding site of their hemagglu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.11.030
更新日期:2007-05-10 00:00:00
abstract::Most adenoviral vectors use in gene therapy protocols derive from species C. However, expression of the primary receptor (human Coxsackievirus and Adenovirus receptor, hCAR) for these AdV is variable on cancer cells. In vivo targeting of a therapeutic gene to specific cells has then become a major issue in gene therap...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.12.023
更新日期:2004-04-10 00:00:00
abstract::Two genetically distinct infectious recombinant virus clones (pMLV, constructed from Ingelvac PRRS MLV and pMN184, constructed from virulent strain MN184) were developed to study attenuation of contemporary porcine reproductive and respiratory syndrome virus (PRRSV) strain MN184. Two reciprocal chimeric clones (pMLVOR...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.09.032
更新日期:2008-02-20 00:00:00
abstract::We have constructed a recombinant baculovirus expressing high levels of the herpes simplex virus type 1 (HSV-1) glycoprotein E (gE) in Sf9 cells. The expressed gE migrated on gels as a double band with apparent molecular weights of 68 and 70 kDa. The recombinant gE was glycosylated based on its susceptibility to tunic...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90500-o
更新日期:1992-06-01 00:00:00
abstract::Brain metastases occur commonly in the setting of a variety of human cancers. At present, such cases are invariably fatal and highlight a need for research on new therapies. We have developed a mouse brain tumor model utilizing the Harding-Passey melanoma cell line injected intracranially into C57Bl/6 mice. Tumors dev...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1382
更新日期:1995-08-01 00:00:00
abstract::The minimal 5' initiation of assembly sequence for papaya mosaic virus (PMV) has been determined by the generation of successive 3' deletion clones from a full-length cDNA copy of the PMV genome followed by in vitro assembly of RNA transcripts. Sequences closest to the 5' terminus were cloned into plasmid vectors with...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1118
更新日期:1994-02-15 00:00:00