Abstract:
:The single-cell ELISPOT assay was used to determine the frequency and isotype commitment of virus-specific antibody-forming cells (AFC) at different anatomical locations following intranasal Sendai virus infection of C57BL/6 and 129/Sv mice. AFC responses in the mediastinal and cervical lymph nodes showed sharp increases and declines, first of IgM AFC, peaking about 7 days after infection, and then of IgG and IgA AFC, peaking about 10 days after infection. A wave of IgM AFC preceding the other isotypes was less evident in the spleen, where peak frequencies of AFC occurred 14 days after infection. Virus-specific AFC appeared in the bone marrow with a unique kinetic pattern, increasing in frequency gradually over the first 3 weeks after infection to a plateau that remained constant. Circulating IgM and IgG achieved significant titers approximately a week after infection; IgM titers were transient, but IgG levels increased sharply and remained high, reflecting the longevity of the bone marrow AFC response. Strain differences in isotype bias were noted, particularly preferential switching to the gamma 2a gene in 129/Sv mice. The B-cell response to acute respiratory viral infection thus exhibits features that are distinct from the primary response to nonreplicating antigens.
journal_name
Virologyjournal_title
Virologyauthors
Sangster M,Hyland L,Sealy R,Coleclough Cdoi
10.1006/viro.1995.1079subject
Has Abstractpub_date
1995-02-20 00:00:00pages
287-91issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(85)71079-3journal_volume
207pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Our previous studies have demonstrated that virus-specific cell-mediated cytotoxicity of sensitized leukocytes can be induced using clonal ginbuna crucian carp and their syngeneic cell lines. In the present study, we attempt to determine if virus-specific cytotoxic cell populations of fish express CD8alpha and TCRbeta...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.01.019
更新日期:2006-05-10 00:00:00
abstract::Rift Valley fever virus-induced protein synthesis was examined by polyacrylamide gel electrophoresis and fluorography. Five virus-induced polypeptides were detected, the nucleocapsid protein N, the nucleus-associated nonstructural protein NS1, the glycoproteins G1 and G2, and a protein of molecular weight 80K. The N, ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90277-0
更新日期:1984-04-15 00:00:00
abstract::We recently reported the phenomenon of retroviral insertion into a herpesvirus (Isfort et al., (1992) Proc. Natl. Acad. Sci. USA 89, 991-995). We have now isolated Marek's disease virus (MDV) clones that carry retroviral inserts. Retroviral LTR insertion has the potential to activate or inactivate herpesvirus genes le...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1018
更新日期:1993-01-01 00:00:00
abstract::The incidence of head and neck squamous cell carcinomas (HNSCCs) is rising in developed countries. This is driven by an increase in HNSCCs caused by high-risk human papillomavirus (HPV) infections or HPV + HNSCCs. Compared to HNSCCs not caused by HPV (HPV- HNSCCs), HPV + HNSCCs are more responsive to therapy and assoc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.07.004
更新日期:2020-09-01 00:00:00
abstract::Vaccinia virus (VV) encodes a 37-kDa envelope protein (p37) that is palmitylated on cysteine residues 185186 of the 372-amino acid protein. We have previously reported on a loosely conserved consensus motif. Further analysis has identified a conserved consensus sequence, Hydro*AAC(C)A (Hydro* represents a hydrophobic ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9543
更新日期:1999-02-01 00:00:00
abstract::The liver has been suspected to be one of the major targets of dengue virus infection. Here, we detected increasing secretion of the chemokine RANTES (regulated upon activation, normal T cell expressed and secreted), which functions to recruit the immune cells, in dengue-virus-infected liver cells and patients. Three ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0524
更新日期:2000-10-10 00:00:00
abstract::Turnip leaves infected with turnip crinkle virus accumulate a 35-kd polypeptide which comigrates with the major protein from isolated virions. RNA from TCV virions directs the synthesis of a number of polypeptides in vitro including a 35-kd protein which is immunoprecipitable with antiserum to virus particles. Transla...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90326-9
更新日期:1982-04-15 00:00:00
abstract::The shuttle vector plasmid pZ189 was used as a target for mutagenesis in COS-1 cells. Complex mutations were analyzed in 5 plasmids that were recovered from noninfected cells and 15 plasmids that were recovered from cells that were infected with herpes simplex virus type-1 (HSV-1). Complex mutations in noninfected cel...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90895-i
更新日期:1991-04-01 00:00:00
abstract::Bovine herpesvirus 1 (BHV-1) gene expression was examined by RNA blot hybridization using clones representing immediate-early, early, and late genes. An immediate-early protein gene probe hybridized with two transcripts, 3.4 and 5.8 kb, expressed by infected cells in the presence of cycloheximide (CH). During infectio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90744-a
更新日期:1992-05-01 00:00:00
abstract::Viruses in certain genera in family Caulimoviridae were shown to integrate their genomic sequences into their host genomes and exist as endogenous pararetroviral sequences (EPRV). However, members of the genus Caulimovirus remained to be the exception and are known to exist only as episomal elements in the infected ce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.03.003
更新日期:2008-07-05 00:00:00
abstract::In order to molecularly characterize rapidly and slowly replicating HIV-1 variants, molecular clones were obtained from a rapid/high virus isolate. This isolate, 4803, had only been passaged in peripheral blood mononuclear cells (PBMC) prior to cloning. Molecular cloning was done in bacteriophage lambda-dash using hig...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90469-r
更新日期:1991-03-01 00:00:00
abstract::Most adenoviral vectors use in gene therapy protocols derive from species C. However, expression of the primary receptor (human Coxsackievirus and Adenovirus receptor, hCAR) for these AdV is variable on cancer cells. In vivo targeting of a therapeutic gene to specific cells has then become a major issue in gene therap...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.12.023
更新日期:2004-04-10 00:00:00
abstract::Aster yellows virus (AYV) was recovered from hemolymph, alimentary canal, salivary glands and ovaries, but not from Malpighian tubules, mycetomes, fat body, testes, or brain of viruliferous leafhoppers, Macrosteles fascifrons (Stål). The presence of virus in the tissues was determined by injecting their extracts into ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(67)90070-0
更新日期:1967-12-01 00:00:00
abstract::CD8 T cells contribute to effective clearance of mouse adenovirus type 1 (MAV-1) and to virus-induced pulmonary inflammation. We characterized effects of a CD8 T cell effector, TNF, on MAV-1 pathogenesis. TNF inhibited MAV-1 replication in vitro. TNF deficiency or immunoneutralization had no effect on lung viral loads...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.05.004
更新日期:2020-08-01 00:00:00
abstract::The 5'-terminal noncoding region sequences were determined for the genome RNAs of seven strains of St. Louis encephalitis virus (SLEV) and one strain of West Nile virus (WNV) using a single synthetic cDNA primer complementary to the 5'-terminus of the coding region of a strain of WNV RNA. The 5'-terminal sequences obt...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90468-0
更新日期:1988-02-01 00:00:00
abstract::The complete genomes of 30 Soybean mosaic virus (SMV) isolates and strains were sequenced in this study. Together with fourteen previously reported sequences, we analyzed the genetic structure of the SMV population. Analyses of genetic diversity showed that different genomic regions of SMV are under different evolutio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.007
更新日期:2009-10-10 00:00:00
abstract::The prototypic macrophage-tropic HIV-1 isolate, HIV-1BaL, cannot replicate in the monocytoid cell line THP-1. After induction of differentiation by a phorbol diester, a fraction of THP-1 cells became permissive to HIV-1BaL. In contrast, this treatment decreased permissiveness for the lymphotropic isolate HIV-1LAI. Vir...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1121
更新日期:1993-03-01 00:00:00
abstract::The function of rice dwarf virus segment 11 and the corresponding segments of other phytoreoviruses is not yet determined. The amino acid sequence of Pns11, encoded by segment 11, contains a putative zinc finger and five flanking basic regions at the C-terminus. The full-length Pns11 protein and three truncated deriva...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8945
更新日期:1998-01-20 00:00:00
abstract::Mouse hepatitis virus (MHV) JHMV and its soluble receptor-resistant (srr) mutants, srr7, srr11, and srr18, grew and induced syncytia equally well in BHK-R1 cells expressing the MHVR1 receptor derived from MHV-susceptible BALB/c mice. In contrast, srr growth and syncytia formations were drastically reduced relative to ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0409
更新日期:2000-07-20 00:00:00
abstract::A full-length cDNA of cucurbit aphid-borne yellows virus (CABYV) has been constructed and expressed either as an in vitro transcript, under control of a bacteriophage T7 RNA polymerase promoter, or in vivo, under control of the cauliflower mosaic virus 35S promoter in an agroinfection vector. The biological activity o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.9945
更新日期:1995-12-01 00:00:00
abstract::The transcriptive complex, the nucleocapsid with the viral RNA-synthesizing activity, of Newcastle disease virus (NDV) contains three protein components, the major structural subunit (NP) and two associated proteins (P and L) involved in the RNA synthesis. We studied the pathway of these proteins from synthesis to ass...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90132-1
更新日期:1985-12-01 00:00:00
abstract::The most recent introduction of an avian influenza A virus without reassortment into mammals occurred in 1979 when H1N1 strains could be isolated from diseased pigs in northern Europe. This newly introduced avian virus formed a stable lineage in pigs and, in the meantime, spread all over Europe. In 1991 highly pathoge...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1344
更新日期:1994-07-01 00:00:00
abstract::Human Papillomavirus infection is highly prevalent worldwide. While most types of HPV cause benign warts, some high-risk types are known to cause cervical cancer, as well as cancer of the oral cavity and head and neck. Persistent cutaneous HPV infection can be particularly problematic in patients with chronic immunosu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.08.007
更新日期:2019-11-01 00:00:00
abstract::In this study we have used an expression system based on Semliki Forest virus (SFV) to study assembly and intracellular localization of certain capsid proteins of rotavirus in neurons and mammalian epithelial cells. The complete genes of vp2 (vp2A) and vp6 (vp6A) of group A rotavirus (SA-11) and gene 5 encoding vp6 (v...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8987
更新日期:1998-03-15 00:00:00
abstract::NS35, a basic protein encoded by gene 8 of SA11 rotavirus, possesses RNA-binding activity and is essential for genome replication. To identify conserved regions in the NS35 gene and its protein product, we determined the nucleotide sequences of the NS35 gene for the mammalian and avian rotaviruses Wa, DS1, SA11 (Patto...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1059
更新日期:1993-02-01 00:00:00
abstract::The influenza virus mRNAs are structurally similar to cellular mRNAs nevertheless; the virus promotes selective translation of viral mRNAs despite the inhibition of host cell protein synthesis. The infection proceeds normally upon functional impairment of eIF4E cap-binding protein, but requires functional eIF4A helica...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.10.028
更新日期:2012-01-20 00:00:00
abstract::The complete nucleotide sequence of apple stem grooving virus (ASGV) genome has been determined. The genome is 6496 nucleotides in length excluding a 3'-terminal poly(A) tail and contains two overlapping open reading frames (ORFs). ORF1 begins at nucleotide position 37 and is terminated at position 6341, encoding a pr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90170-t
更新日期:1992-11-01 00:00:00
abstract::Five revertants of a linker-scanning mutation adjacent to the stem-loop V attenuation determinant (X472) in the 5' noncoding region of poliovirus RNA were independently isolated from neuroblastoma cells and contained RNAs with seven nucleotide changes in the pyrimidine-rich region. Generation of the identical rare sec...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9981
更新日期:1999-11-25 00:00:00
abstract::A minor core protein, VP4, of bluetongue virus serotype 10 (BTV-10) has been synthesized in insect cells infected with a genetically manipulated recombinant baculovirus. When insect cells were coinfected by this recombinant virus and a recombinant baculovirus expressing the two major core proteins (VP3 and VP7) of the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90600-t
更新日期:1992-08-01 00:00:00
abstract::The DNA sequence of the gag and pol regions of a provirus cloned from a bovine tumor is presented. In order to confirm these results the sequence of portions of a second clone, derived from a virus-producing cell line, was also determined. The gag gene was found to consist of 1179 nucleotides, which probably encode on...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90344-7
更新日期:1985-04-30 00:00:00