Abstract:
:This work investigated whether the Escherichia coli RNase III gene, rnc+, could complement vp1080, a mutant vaccinia virus that is deleted of its E3L gene. Like E3L, rnc+ codes for a dsRNA binding protein that contains an additional nucleolytic activity. Rnc genes were cloned into the eukaryotic expression vector pMTVa-, expressed in COS-1 cells, and shown to be functional. Transient rescue experiments in HeLa cells demonstrated that the cleavage function of the rnc+ gene was necessary for full rescue of vp1080. The rnc 70 gene, which encodes a product deficient in catalytic activity but still capable of binding to dsRNA, rescued vp1080 weakly. The rnc 105 gene, which encodes a product that cannot bind or cleave dsRNA, was unable to rescue vp1080. The rnc genes were also inserted into the E3L locus of vp1080. While recombinants containing the rnc+ gene or the rnc 70 gene regained the IFN resistance phenotype in RK13 cells, full host range of vaccinia virus was only restored in the recombinant containing the rnc+ gene. Thus, the ability of RNase III to process dsRNA appears to be necessary to restore the host range phenotype. The vp-rnc 105 recombinant behaved similarly to vp1080.
journal_name
Virologyjournal_title
Virologyauthors
Shors T,Jacobs BLdoi
10.1006/viro.1996.8319subject
Has Abstractpub_date
1997-01-06 00:00:00pages
77-87issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(96)98319-1journal_volume
227pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Old world hantaviruses, causing hemorrhagic fever with renal syndrome (HFRS), still present a public health problem in Asia and Eastern Europe. The majority of cases has been recorded in China. The aim of our study was to generate human recombinant neutralizing antibodies to a hantavirus by phage display technology. T...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(02)00094-6
更新日期:2003-03-30 00:00:00
abstract::Six deletion mutations and an insertion were generated in the env gene of cloned copies of Moloney murine leukemia virus DNA. All seven mutants were replication-defective as tested by transformation of NIH/3T3 cells. The mutant DNAs were introduced into NIH/3T3 cells to generate stable producer lines; all released vir...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90983-i
更新日期:1991-08-01 00:00:00
abstract::Spindle-shaped viruses are a dominant morphotype in hypersaline waters but their molecular characteristics and their relationship to other archaeal viruses have not been determined. Here, we describe the isolation, characteristics and genome sequence of His2, a spindle-shaped halovirus, and compare it to the previousl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.02.005
更新日期:2006-06-20 00:00:00
abstract::The V protein of Sendai virus (SeV) is nonessential for virus replication in cell culture but indispensable for viral pathogenicity in mice. At the C terminus of the V protein, there are amino acid residues conserved among the members of the Paramyxovinae subfamily that are clustered in three regions: region I, just d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1516
更新日期:2002-08-01 00:00:00
abstract::Influenza viruses of the H3N2 subtype similar to Aichi/2/68 and Victoria/3/75 persist in pigs many years after their antigenic counterparts have disappeared from humans (Shortridge et al. (1977). Science 19, 1454-1455). To provide information on the mechanism of conservation of these influenza viruses in pigs, the hem...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90405-9
更新日期:1988-01-01 00:00:00
abstract::The protruding (P) domain of norovirus VP1 is responsible for immune recognition and host receptor interaction. Our previous studies have demonstrated that a modification of the ends of the P domain affects the conformation and/or function of the P protein. An expression of the P domain with or without the hinge, or w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.11.017
更新日期:2011-02-20 00:00:00
abstract::HIV-1 envelope glycoproteins (Env) and Env-based immunogens usually do not interact efficiently with the inferred germline precursors of known broadly neutralizing antibodies (bNAbs). This deficiency may be one reason why Env and Env-based immunogens are not efficient at inducing bNAbs. We evaluated the binding of 15 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.08.002
更新日期:2015-12-01 00:00:00
abstract::Potato virus S (PVS) is a major plant pathogen that causes considerable losses in global potato production. Knowledge of the evolutionary history and spatio-temporal dynamics of PVS is vital for developing sustainable management schemes. In this study, we investigated the phylodynamics of the virus by analysing 103 nu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.09.022
更新日期:2018-12-01 00:00:00
abstract::After infection with mumps virus, cellular ribonucleic acid synthesis of a murine lymphoma cell line, EL4, was appreciably depressed. The inactivation of viral infectivity by ultraviolet irradiation or the treatment of cells with mouse interferon did not abolish the inhibiting effect, suggesting that virus replication...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90187-9
更新日期:1984-06-01 00:00:00
abstract::The main route of small ruminant lentivirus dissemination is the ingestion of infected cells present in colostrum and milk from infected animals. However, whether only macrophages or other cell subtypes are involved in this transmission is unknown. We derived epithelial cell cultures, 100% cytokeratin positive, from m...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9752
更新日期:1999-06-20 00:00:00
abstract::Human Adenovirus Type 4 (HAdV-4) is responsible for epidemic outbreaks of Acute Respiratory Disease (especially in military recruits), and is known to cause significant morbidity with several reported cases of mortality. However, we do not understand why this serotype causes such high morbidity, and have little insigh...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.01.017
更新日期:2008-05-10 00:00:00
abstract::HTLV-I infection is characterized by low viremia and restricted viral expression. While the mechanisms regulating viral latency are poorly understood, it is believed that interactions between viral and host cellular factor(s) are involved. Several lines of evidence indicate that HTLV-I provirus may be methylated in pr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90649-v
更新日期:1991-05-01 00:00:00
abstract::We investigated whether culture conditions could affect the RANTES antiviral effect on human immunodeficiency virus type 1 (HIV-1) infection of primary macrophages. Monocyte-derived macrophages (MDM) were obtained either as (1) the adherent cells of 5-day cultures of blood mononuclear cells (PBMC), followed by 2 days ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9452
更新日期:1998-12-05 00:00:00
abstract::Plant virus-based vectors provide valuable tools for expression of foreign proteins in plants and for gene function studies. None of the presently available virus vectors is suitable for use in soybean. In the present study, we produced Bean pod mottle virus (BPMV)-based vectors that are appropriate for gene expressio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.08.046
更新日期:2006-01-20 00:00:00
abstract::To investigate the role of alfalfa mosaic virus coat protein (CP) in genome activation, asymmetric plus-strand RNA accumulation, and cell-to-cell spread of the virus, mutations were made in the CP gene and putative CP binding sites in the 3'-untranslated region (UTR) of RNA 3. Mutants that produced no CP-related pepti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1411
更新日期:1994-08-01 00:00:00
abstract::It is now well recognized that mutations in the hepatitis B virus (HBV) genome occur during the natural course of chronic viral infection. Regions of the viral genome that are frequently affected by such mutations, rearrangements, and/or deletions generally involve the precore promoter, precore, and core as well as th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8594
更新日期:1997-07-07 00:00:00
abstract::HPV-16 is the major causes of cervical cancer. Persistence of infection is a necessary event for progression of the infection to cancer. Among other factors, virus persistence is due the viral proteins fighting the immune response. HPV-16 E5 down-regulates MHC/HLA class I, which is much reduced on the cell surface and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.07.044
更新日期:2010-11-10 00:00:00
abstract::IL-17-producing (Th17) and regulatory T (Treg) cells have been well established in the pathogenesis of many inflammatory diseases. To assess whether Th17 and Treg were altered in acute virus-induced myocarditis (AVMC) mice, we assessed Th17/Treg functions on different levels in AVMC. It was shown that the expression o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.09.006
更新日期:2011-12-05 00:00:00
abstract::The spread of HIV-1 to the nervous system occurs early during infection in a large number of asymptomatic virus carriers. In order to address the question whether the brain is targeted by a group of HIV-1 variants with increased neurotropic properties we have obtained 20 paired isolates from the blood and cerebrospina...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1503
更新日期:1993-10-01 00:00:00
abstract::Two mutant strains of cucumber mosaic virus (CMV) were investigated with respect to virion stability and molecular determinants of aphid vector transmission. The mutant 2A1-MT-60x, derived from the mechanically passaged wild type 2A1-AT, is poorly transmissible by the aphid Aphis gossypii and not transmissible by the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0569
更新日期:2000-10-25 00:00:00
abstract::Lactate dehydrogenase-elevating virus (LDV) is currently classified within the Togaviridae family. In an effort to obtain further information on the characteristics of this virus, we have begun to sequence the viral RNA genome and to map the virion structural protein genes. A sequence of 1064 nucleotides, which repres...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90546-4
更新日期:1990-08-01 00:00:00
abstract::HCV genotype is a major determinant of clinical outcome, and GT1b HCV infection is the most difficult to treat and also the predominant genotype in East Asia and Europe. We developed 1b/JFH-1 inter-genotypic recombinants containing the structural genes (Core, E1, E2), p7 and the 1stTMD of NS2 directly from GT1b clinic...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.04.030
更新日期:2013-08-15 00:00:00
abstract::The APOBEC3 enzyme family are host restriction factors that induce mutagenesis of HIV-1 proviral genomes through the deamination of cytosine to form uracil in nascent single-stranded (-)DNA. HIV-1 suppresses APOBEC3 activity through the HIV-1 protein Vif that induces APOBEC3 degradation. Here we compared two common po...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.11.004
更新日期:2019-01-15 00:00:00
abstract::Peste des petits ruminants (PPR) is a viral disease of goats and sheep characterized by erosive stomatitis, enteritis, and pneumonia. The virus is a member of the family Paramyxoviridae and the genus Morbillivirus. The disease has high morbidity and mortality rates and has a substantial economic impact in developing c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1210
更新日期:1995-04-20 00:00:00
abstract::Within immunocompromised populations, the JC polyomavirus is the cause of the often-fatal disease Progressive Multifocal Leukoencephalopathy (PML). JC virus encodes a protein, termed T-antigen (T-ag), which is essential for its replication and pathogenicity. Previous studies of JCV T-ag have, in general, used antibodi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.07.006
更新日期:2016-10-01 00:00:00
abstract::The nucleotide and deduced amino acid sequence of the gene 4 of murine rotavirus strain Eb were determined. The gene is 2359 nucleotides in length and encodes for a protein of 775 amino acids. Comparison of the VP4 amino acid sequence of the Eb strain with several human and animal rotavirus strains which represent all...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1153
更新日期:1994-03-01 00:00:00
abstract::The PBj14 isolate of simian immunodeficiency virus, SIVsmmPBj14, is an acutely pathogenic lentivirus that causes severe gastrointestinal disease in macaque monkeys. The studies reported here examine the basis for the enteropathic phenotype of SIVsmmPBj14, using flow cytometric analysis of cultured macaque lymphocytes ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0010
更新日期:1996-01-01 00:00:00
abstract::The nucleotide sequence of the gene immediately following the nucleocapsid protein gene of sonchus yellow net virus (SYNV), a plant rhabdovirus, is presented. Serological reactions of SYNV proteins with antibodies elicited by a fusion protein constructed from the sequenced gene indicate that this gene encodes an SYNV ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90190-5
更新日期:1987-11-01 00:00:00
abstract::The role of anti-viral antibodies in homologous protective immunity to a virulent African swine fever virus (ASFV) strain E75 was examined by passive transfer experiments in swine. Eighty-five percent of animals (n = 14) that received anti-ASFV immunoglobulin (Ig) survived challenge infection, while 100% mortality was...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1040
更新日期:1994-01-01 00:00:00
abstract::Re-emergence of ZIKV has caused infections in more than 1.5 million people. The molecular mechanism and pathogenesis of ZIKV is not well explored due to unavailability of adequate model and lack of publically accessible resources to provide information of ZIKV-Human protein interactome map till today. This study made ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.11.007
更新日期:2018-01-15 00:00:00