Abstract:
:Flock house virus (FHV) is the best studied member of the Nodaviridae, a family of small, nonenveloped, isometric RNA viruses of insects and fish. Nodavirus genomes comprise two single-stranded positive-sense RNA segments (RNAs 1 and 2) that encode the viral RNA-dependent RNA polymerase (RdRp) and capsid protein precursor, respectively. The RdRp replicates both genomic RNAs and also generates a subgenomic RNA (RNA3) that is not encapsidated. Although genomic RNAs replicate through negative-sense intermediates, little is known about these RNAs or the details of the replication mechanism. Negative-sense RNAs 1, 2, and 3, as well as putative dimers of RNAs 2 and 3, have been detected in previous studies. In this study we detected dimers of RNAs 1, 2, and 3 by Northern blot analyses of RNA samples from FHV-infected Drosophila cells, as well as from mammalian and yeast cells supporting FHV RNA replication. Characterization of these RNA species by RT-PCR and sequence determination showed that they contained head-to-tail junctions of FHV RNAs. RNAs containing the complete sequence of RNA2 joined to RNA3 were also detected during replication. To examine the template properties of these dimeric RNAs, we made corresponding cDNAs and transcribed them from a T7 promoter in mammalian cells constitutively expressing T7 RNA polymerase, together with RNA1 to provide the RdRp. Although heterologous terminal extensions inhibit FHV RNA replication, monomeric RNA2 was resolved and replicated from complete or partial homodimer templates and from an RNA2-RNA3 heterodimer.
journal_name
Virologyjournal_title
Virologyauthors
Albariño CG,Price BD,Eckerle LD,Ball LAdoi
10.1006/viro.2001.1125subject
Has Abstractpub_date
2001-10-25 00:00:00pages
269-82issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(01)91125-0journal_volume
289pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::We cloned the complete complementary DNA of an isolate of the hepatitis C virus, HCV-S1, into a tetracycline-inducible expression vector and stably transfected it into two human hepatoma cell lines, Huh7 and HepG2. Twenty-six Huh7 and two HepG2-positive clones were obtained after preliminary screening. Two Huh7 (SH-7 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1687
更新日期:2002-11-10 00:00:00
abstract::Hepatitis C virus (HCV) encodes a polyprotein that is processed to produce the structural and nonstructural proteins of the virus. Nonstructural protein 3 (NS3) is a serine proteinase that cleaves the polyprotein to release the NS4A, NS4B, NS5A, and NS5B proteins. To characterize the substrate specificity of NS3, we s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1520
更新日期:1994-10-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) glycoproteins K and B (gK and gB) are intimately involved in virus-induced fusion of cells. Certain mutations in the UL27 (gB) and UL53 (gK) genes confer a syncytial (syn) phenotype characterized by extensive fusion of infected cells and giving rise to multinucleated cells. We have ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1507
更新日期:1993-10-01 00:00:00
abstract::Efferocytosis, the phagocytic clearance of apoptotic cells, can provide host protection against certain types of viruses by mediating phagocytic clearance of infected cells undergoing apoptosis. It is known that HIV-1 induces apoptosis and HIV-1-infected cells are efferocytosed by macrophages, although its molecular m...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.12.025
更新日期:2018-02-01 00:00:00
abstract::Monoclonal antibodies were used to compare the antigenicities of the haemagglutinins of two receptor binding mutants of X-31 (H3N2) influenza virus. The mutants which differed from each other in recognizing sialic acid in either alpha 2-6 linkage or alpha 2-3 linkage to galactose also differed exclusively at residue 2...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90158-2
更新日期:1984-10-15 00:00:00
abstract::The replicative form (RF) of spiroplasma virus 4 (SpV4) has been purified from infected cells of Spiroplasma melliferum strain G1 by alkaline lysis followed by low melting point agarose gel electrophoresis. A partial restriction map has been established. The circular RF was linearized by cutting at the unique ClaI res...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90060-7
更新日期:1986-06-01 00:00:00
abstract::Nucleotide sequence determination of the coat protein cistron of the alfalfa mosaic virus (AIMV) temperature-sensitive mutant, Tbts 7 (uv) revealed a small number of point mutations of which only one results in the replacement of an amino acid: the asparagine residue at position 126 is replaced by an aspartate residue...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90609-0
更新日期:1989-08-01 00:00:00
abstract::New sequences have been obtained by successive overlapping RT-PCR extensions from the pol region of a retroviral RNA (multiple sclerosis-associated retroviral element, MSRV) amplified in retrovirus-like particles from patients with multiple sclerosis. gag and pol sequences are related to type C oncoviruses, whereas th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9792
更新日期:1999-07-20 00:00:00
abstract::ZEBRA, a transcription factor and DNA replication protein encoded by the Epstein-Barr virus (EBV) BZLF1 gene, plays indispensable roles in the EBV lytic cycle. We recently described the phenotypes of 46 single amino acid substitutions introduced into the DNA-recognition region of ZEBRA [Heston, L., El-Guindy, A., Coun...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.09.009
更新日期:2008-12-20 00:00:00
abstract::The mature flavivirus capsid protein (virion C) is commonly thought to be free in the cytoplasm of infected cells and to form a nucleocapsid-like complex with genomic RNA in mature virus particles. There is little sequence conservation among flavivirus virion C proteins, but they are similar in size (e.g., 99 amino ac...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8608
更新日期:1997-06-23 00:00:00
abstract::West Nile virus (WNV) is a mosquito-borne flavivirus that causes febrile illness, encephalitis, and occasionally death in humans. The envelope protein is the main component of the WNV virion surface, and domain III of the envelope protein (EIII) is both a putative receptor binding domain and a target of highly specifi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.05.024
更新日期:2016-09-01 00:00:00
abstract::Polyribosomal RNA from tobacco infected with sonchus yellow net virus (SYNV) contained sequences which hybridized to 125I-labeled SYNV RNA and which were complementary to 80 to 100% of the viral RNA genome. The poly(A)-containing RNA from polyribosomes was complementary to over 90% of the viral genome but the polyribo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90375-1
更新日期:1979-08-01 00:00:00
abstract::Human parainfluenza virus type 1 (hPIV1) and type 3 (hPIV3) initiate infection by sialic acid binding. Here, we investigated sialic acid linkage specificities for binding and infection of hPIV1 and hPIV3 by using sialic acid linkage-modified cells treated with sialidases or sialyltransferases. The hPIV1 is bound to on...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.07.033
更新日期:2014-09-01 00:00:00
abstract::The vaccinia virus temperature-sensitive mutations Cts6 and Cts9 were mapped by marker rescue and DNA sequencing to the A28 gene. Cts6 and Cts9 contain an identical 2-bp deletion truncating the A28 protein and removing the fourth conserved cysteine near the C-terminus. Cts9 mutant virions produced at 40 degrees C were...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.03.060
更新日期:2007-09-15 00:00:00
abstract::Two bipartite begomoviruses, Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV), have been isolated from mosaic-diseased cassava originating from central India and Sri Lanka, respectively. ICMV was transmitted with low efficiency from cassava to Nicotiana benthamiana by sap inoculation to g...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1251
更新日期:2002-02-01 00:00:00
abstract::The allele Pp of gene ref(2)P of Drosophila melanogaster is known to inhibit the multiplication of certain strains of Sigma virus. From a viral strain for which the allele PP of ref(2)P is nonpermissive, host adapted (haP) mutants were selected after mutagenesis in vivo. Mutagenized virus was extracted from infected f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90227-0
更新日期:1981-10-31 00:00:00
abstract::Marek's disease (MD) infection has been controlled effectively by vaccination using nononcogenic and/or attenuated oncogenic Marek's disease virus (MDV) vaccines. Thus far, there is little knowledge on the role of cell-mediated immune (CMI) responses during MDV infection or vaccination. To elucidate the importance of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0400
更新日期:1996-08-01 00:00:00
abstract::The simian hemorrhagic fever virus (SHFV) genome differs from those of other members of the family Arterivirus in encoding two adjacent sets of four minor structural protein open reading frames (ORFs). A stable, full-length, infectious SHFV-LVR cDNA clone was constructed. Virus produced from this clone had replication...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.06.001
更新日期:2014-08-01 00:00:00
abstract::The complete sequence of the genome of the cherry strain of tomato bushy stunt virus (TBSV), a member of the tombusvirus group, was determined. A full-length clone of the genome containing a bacteriophage T7 RNA polymerase promoter was assembled from partial cDNA clones. In vitro transcripts of the genome, either with...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90468-7
更新日期:1990-07-01 00:00:00
abstract::We described a simple method to introduce site-specific mutations into region E3 of adenovirus (Ad). Mutations are made in cloned Ad2 EcoRI-D (map position 76-83), then ligated between Ad5 EcoRI-A (map position 0-76) and EcoRI-B (map position 83-100) to complete the viral genome. We have used this method to isolate a ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90412-5
更新日期:1986-01-15 00:00:00
abstract::The SV40 large T-antigen (TAg) has proven useful in studying pathways involved with cell division and tissue homeostasis. TAg disrupts the normal action of tumor suppressors pRb and p53. It is unclear whether T-antigen inhibition of p53 and pRb is sufficient for oncogenic transformation or if additional T-antigen acti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0866
更新日期:2001-04-25 00:00:00
abstract::Influenza antiviral drugs that use protein inhibitors can lose their efficacy as resistant strains emerge. As an alternative strategy, we investigated the use of small interfering RNA molecules (siRNAs) by characterizing three siRNAs (M747, M776 and M832) targeting the influenza matrix 2 gene and three (NS570, NS595 a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.07.019
更新日期:2016-10-01 00:00:00
abstract::Measles virus (MV) inhibits lymphocyte function in patients, as well as in cells infected in vitro. The proliferation of phytohemagglutinin-stimulated T lymphocytes is suppressed by in vitro MV infection, as shown by the diminished incorporation of [3H]thymidine into DNA and the reduced frequency of cells in the S pha...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90316-h
更新日期:1992-03-01 00:00:00
abstract::Human papillomavirus (HPV) E6 oncoproteins target numerous cellular proteins for ubiquitin-mediated degradation. In the case of p53 this is mediated by the E6AP ubiquitin ligase. However, there are conflicting reports concerning how central E6AP is to the global function of the HPV-16 and HPV-18 E6 oncoproteins. To in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.029
更新日期:2009-10-10 00:00:00
abstract::Human immunodeficiency virus (HIV) encodes a transacting protein, Rev, which interacts with an RNA element (RRE) to mediate nuclear export of unspliced viral mRNA. Recently, the RNA constitutive transport elements (CTE) from Mason-Pfizer monkey virus (MPMV) and simian retrovirus type I (SRV-1) were shown to render Rev...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8407
更新日期:1997-02-17 00:00:00
abstract::The protein content of vaccinia virus mature virions, purified by rate zonal and isopycnic centrifugations and solubilized by SDS or a solution of urea and thiourea, was determined by the accurate mass and time tag technology which uses both tandem mass spectrometry and Fourier transform-ion cyclotron resonance mass s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.08.025
更新日期:2007-02-05 00:00:00
abstract::A clinical feature of Ross River virus disease (RRVD) is the periodic relapse of symptoms months after the initial onset of disease. The underlying mechanisms responsible for this relapse have not been determined. In a long-term (148 days) in vitro study of persistently infected murine macrophages we established that ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1587
更新日期:2002-09-30 00:00:00
abstract::Following CD4 receptor binding to the HIV-1 envelope spike (Env), the conserved N-heptad repeat (NHR) region of gp41 forms a coiled-coil that is a precursor to the fusion reaction. Although it has been a target of drug and vaccine design, there are few monoclonal antibody (mAb) tools with which to probe the antigenici...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.04.005
更新日期:2008-07-20 00:00:00
abstract::A series of carboxy- and amino-terminal deletion mutations in the movement protein (MP) gene of tobacco mosaic virus (TMV) were ligated into a cloned TMV cDNA deleted for the endogenous MP gene. RNA transcripts were produced in vitro from clones carrying the various mutated MP genes. The effect of the deletion mutatio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90452-u
更新日期:1992-04-01 00:00:00
abstract::Astrocytes protect neurons but also evoke a proinflammatory response to injury and viral infections including HIV. We investigated the mechanism of HIV-1 infection in primary astrocytes, which showed minimal but productive viral infection independent of CXCR4. As with ectopic-CD4-expressing astrocytes, lysosomotropic ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.03.002
更新日期:2014-05-01 00:00:00