Abstract:
:While several studies have shown a role for autophagy in the replication of dengue virus (DENV), these studies have been performed in directly infected cells. However, in severe cases of DENV infection the critical cell in the disease is believed to be monocytes which are poorly infected directly, but are highly susceptible to antibody enhanced infection. This study sought to determine the involvement of autophagy in the DENV infection of monocytic cells, using U937 cells as a model system. While the induction of autophagy was seen in response to DENV-2 infection, biochemical induction of autophagy resulted in a significant decrease in virus output. Down regulation of autophagy resulted in only a very slight increase in intracellular virus levels. In monocytic cells autophagy is not a significant part of the DENV replication mechanism, and there are distinct cell type specific differences in the DENV-autophagy interaction.
journal_name
Virologyjournal_title
Virologyauthors
Panyasrivanit M,Greenwood MP,Murphy D,Isidoro C,Auewarakul P,Smith DRdoi
10.1016/j.virol.2011.07.010subject
Has Abstractpub_date
2011-09-15 00:00:00pages
74-84issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(11)00316-3journal_volume
418pub_type
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