Porcine sapelovirus enters PK-15 cells via caveolae-dependent endocytosis and requires Rab7 and Rab11.

abstract：:The group B noncultivatable rotavirus, ADRV, was purified from infected stool specimens. Double-stranded RNA was extracted, polyadenylated, reverse transcribed into cDNA, and cloned into plasmid vector pAT153. Each cDNA clone hybridized to a single ADRV RNA segment and cDNA clones of each genomic RNA segment were iden...

journal_title：Virology

authors： Chen GM,Hung T,Mackow ER

更新日期：1990-04-01 00:00:00

abstract：:The genome of peanut clump pecluvirus (PCV) consists of two messenger RNA components which contain, respectively, three and five open reading frames (ORFs). Inoculation of transcripts from full-length cDNA clones derived from the PCV RNAs showed that RNA-1 is able to replicate in the absence of RNA-2 in protoplasts, b...

journal_title：Virology

authors： Herzog E,Hemmer O,Hauser S,Meyer G,Bouzoubaa S,Fritsch C

更新日期：1998-09-01 00:00:00

abstract：:A recombinant vaccinia virus that expresses the nucleoprotein gene of Lassa virus (Josiah strain) under the control of the P7.5 promoter was constructed using the lacZ coexpression transfer vector pSC11. Southern blot analysis demonstrated that recombination of the sequences inserted within the thymidine kinase gene o...

journal_title：Virology

authors： Morrison HG,Bauer SP,Lange JV,Esposito JJ,McCormick JB,Auperin DD

更新日期：1989-07-01 00:00:00

abstract：:Adeno-associated viruses (AAVs) are leading candidate vectors for human gene therapy. AAV serotypes have broad cellular tropism and use a variety of cellular receptors. AAV serotype 3 binds to heparan sulfate proteoglycan prior to cell entry and is serologically distinct from other serotypes. The capsid features that ...

journal_title：Virology

authors： Lerch TF,Xie Q,Chapman MS

更新日期：2010-07-20 00:00:00

abstract：:Following a bite from an infected tick, tick-borne flaviviruses cause encephalitis, meningitis and hemorrhagic fever in humans. Although these viruses spend most of their time in the tick, little is known regarding the virus-vector interactions. We developed a simple method for synchronously infecting Ixodes scapulari...

journal_title：Virology

authors： Mitzel DN,Wolfinbarger JB,Long RD,Masnick M,Best SM,Bloom ME

更新日期：2007-09-01 00:00:00

abstract：:The extent of viral DNA methylation in non-virus-producing benign and malignant tumors induced by the Shope (rabbit) papilloma virus was investigated with the two isoschizomeric restriction endonucleases MspI and HpaII. The overall extent of methylation is variable in papillomas and uniformly higher in carcinomas. In ...

journal_title：Virology

authors： Wettstein FO,Stevens JG

更新日期：1983-04-30 00:00:00

abstract：:Viruses in certain genera in family Caulimoviridae were shown to integrate their genomic sequences into their host genomes and exist as endogenous pararetroviral sequences (EPRV). However, members of the genus Caulimovirus remained to be the exception and are known to exist only as episomal elements in the infected ce...

journal_title：Virology

authors： Pahalawatta V,Druffel K,Pappu H

更新日期：2008-07-05 00:00:00

abstract：:The close association between human papillomavirus type 16 and cervical cancer implies some role for the virus in the development of this cancer. It has been demonstrated that HPV-16 DNA sequences in the presence of activated ras are capable of transforming primary baby rat kidney cells. This communication describes t...

journal_title：Virology

authors： Banks L,Crawford L

更新日期：1988-07-01 00:00:00

abstract：:Coronavirus E protein is a small viral envelope protein that plays an essential role in coronavirus assembly; coexpression of coronavirus M and E proteins results in the production of virus-like particles. The present study demonstrated that mouse hepatitis virus (MHV) E protein was released as an integral membrane pr...

journal_title：Virology

authors： Maeda J,Maeda A,Makino S

更新日期：1999-10-25 00:00:00

abstract：:We studied experimental canine oral papillomavirus (COPV) infection by in situ hybridization and immunohistochemistry of weekly biopsies. After 4 weeks, viral DNA in rete ridges suggested a keratinocyte stem cell target. Abundant viral DNA was seen in E4-positive cells only. E4 was predominantly cytoplasmic but also n...

journal_title：Virology

authors： Nicholls PK,Doorbar J,Moore RA,Peh W,Anderson DM,Stanley MA

更新日期：2001-05-25 00:00:00

abstract：:African swine fever virus (ASFV) growth and plaque formation were inhibited by phosphonoacetic acid (PAA) concentrations of 200 micrograms/ml or more. One spontaneous mutant and two mutants isolated from mutagenized virus were resistant to PAA inhibition and showed practically normal viral DNA synthesis in the presenc...

journal_title：Virology

authors： Marques MI,Costa JV

更新日期：1995-12-01 00:00:00

abstract：:The nucleocapsid (N) protein genes of vesicular stomatitis virus, serotypes New Jersey and Indiana, were expressed in Escherichia coli BL21(DE3) transformed by the recombinant expression vector pET-3a containing N genes under the control of the T7 RNA polymerase promoter. Following induction with isopropylthiogalactop...

journal_title：Virology

authors： Das T,Banerjee AK

更新日期：1993-03-01 00:00:00

abstract：:The open reading frame of the large subunit (R1) of herpes simplex virus type 1 (HSV-1) ribonucleotide reductase has been positioned downstream of the phage T7 gene 10 promoter in the expression vector, pET. Transformation of this recombinant plasmid into Escherichia coli BL21 DE3 cells containing the T7 RNA polymeras...

journal_title：Virology

authors： Furlong J,Conner J,McLauchlan J,Lankinen H,Galt C,Marsden HS,Clements JB

更新日期：1991-06-01 00:00:00

abstract：:The bacteriophage T4 e gene encodes lysozyme (e-lysozyme), which releases progeny phage after normal infection of Escherichia coli cells. A mutation in the spackle gene suppresses the defect in e-lysozyme (Emrich, 1968). The spackle gene was mapped between genes 41 and 61, but its precise location has not previously b...

journal_title：Virology

authors： Kai T,Ueno H,Otsuka Y,Morimoto W,Yonesaki T

更新日期：1999-08-01 00:00:00

abstract：:Infection of different human epithelial cell lines with human respiratory syncytial virus (HRSV) revealed significant differences in the electrophoretic mobility of the viral attachment glycoprotein (G). Cell-type specific differences in G protein glycosylation were observed with certain lectins and sugar-specific rea...

journal_title：Virology

authors： García-Beato R,Martínez I,Francí C,Real FX,García-Barreno B,Melero JA

更新日期：1996-07-15 00:00:00

abstract：:We previously demonstrated that cellular mRNAs are degraded in CD4 positive lymphocytes infected by the human immunodeficiency virus, HIV-1, but not in cells infected by the simian lentivirus, SIV. To begin to define the molecular mechanisms underlying this RNA degradation, we have established an in vitro RNA degradat...

journal_title：Virology

authors： Agy MB,Sherbert CH,Katze MG

更新日期：1996-03-01 00:00:00

abstract：:Coinfection of tobacco plants with potato virus X (PVX) and any of several members of the potyvirus group causes a synergistic disease characterized by a dramatic increase in symptom severity correlated with a 3- to 10-fold increase in the accumulation of PVX in the first systemically infected leaves. We have recently...

journal_title：Virology

authors： Shi XM,Miller H,Verchot J,Carrington JC,Vance VB

更新日期：1997-04-28 00:00:00

abstract：:The sequences of 189 residues from the 3' terminus of three RNAs of one strain and two RNAs of another strain of tomato aspermy virus have been determined; there was almost complete sequence homology between the RNAs. A base-paired transfer RNA-like structure is proposed for tomato aspermy virus RNAs which is similar ...

journal_title：Virology

authors： Wilson PA,Symons RH

更新日期：1981-07-15 00:00:00

abstract：:Bovine cell lines individually expressing two related herpesviral proteins, pseudorabies virus glycoprotein 50 and herpes simplex virus type 1 glycoprotein D, were examined for their susceptibility/resistance to infection with several alphaherpesviruses. Cell lines expressing gp50 or gD-1 resisted plaque formation by ...

journal_title：Virology

authors： Chase CC,Lohff C,Letchworth GJ 3rd

更新日期：1993-05-01 00:00:00

abstract：:Aside from a common gene organization shared with other picornaviruses, hepatitis A virus (HAV) is characterized by its slow-growth phenotype, the inability to shut off host macromolecular synthesis, and, in general, lack of cytopathic (cp) effects in permissive cell cultures. Nevertheless, several cp HAV strains have...

journal_title：Virology

authors： Gosert R,Egger D,Bienz K

更新日期：2000-01-05 00:00:00

abstract：:Enterovirus A71 (EV-A71) is a neurotropic enterovirus that uses heparan sulfate as an attachment receptor. The molecular determinants of EV-A71-heparan sulfate interaction are unknown. With In silico heparin docking and mutagenesis of all possible lysine residues in VP1, we identified that K162, K242 and K244 are resp...

journal_title：Virology

authors： Tan CW,Sam IC,Lee VS,Wong HV,Chan YF

更新日期：2017-01-15 00:00:00

abstract：:We used a mouse nasal model of herpes simplex virus 2 (HSV-2) infection to examine the biological properties of HSV-2 wild-type (wt), TK-negative, and replication-defective strains in vivo. Nasal septa tissue is the major site of wt viral replication post intranasal (i.n.) inoculation. The HSV-2 strain 186 syn(+)-1 wt...

journal_title：Virology

authors： Jones CA,Taylor TJ,Knipe DM

更新日期：2000-12-05 00:00:00

abstract：:HeLa cell extracts containing wild-type copies of the minute virus of mice NS-1 polypeptide produced from a recombinant vaccinia virus vector could support the excision and replication of viral 5' telomeres from cloned concatemer junction fragments. Resolution did not occur if wild-type NS-1 was omitted from the extra...

journal_title：Virology

authors： Cotmore SF,Nuesch JP,Tattersall P

更新日期：1992-09-01 00:00:00

abstract：:Hematopoietic tissues obtained from avian leukosis virus (ALV)-infected Hyline SC chickens were analyzed for the presence of integrated viral DNA sequences. Cells were prepared from bone marrow, bursa, spleen, thymus, and peripheral blood. Following the removal of erythrocytes, cellular DNAs from each of these tissues...

journal_title：Virology

authors： Baba TW,Humphries EH

更新日期：1986-12-01 00:00:00

abstract：:Entry determinants in the XPR1 receptor for the xenotropic/polytropic mouse leukemia viruses (XP-MLVs) lie in its third and fourth putative extracellular loops (ECLs). The critical ECL3 receptor determinant overlies a splice donor and is evolutionarily conserved in vertebrate XPR1 genes; 2 of the 3 rare replacement mu...

journal_title：Virology

authors： Lu X,Martin C,Bouchard C,Kozak CA

更新日期：2014-11-01 00:00:00

abstract：:Simian-human immunodeficiency virus encoding both reverse transcriptase (RT) and envelope genes of HIV-1 (RT Env SHIV) is important for evaluating biomedical prevention modalities for HIV/AIDS. We describe virological characterization of a clade B RT Env SHIV following infection of macaques via multiple routes. In viv...

journal_title：Virology

authors： Pal R,Galmin L,Pereira LE,Li B,Zhang J,Li D,Francis J,McNicholl JM,Weiss DE,Smith JM

更新日期：2012-10-10 00:00:00

abstract：:RNase protection analysis of mRNAs for Epstein-Barr virus (EBV) genes activated early in the induction of the productive cycle in Akata cells has confirmed that BZLF1 and BRLF1 behave as immediate early genes. These are likely to be the only virus immediate early genes since analysis of three other transcripts previou...

journal_title：Virology

authors： Packham G,Brimmell M,Cook D,Sinclair AJ,Farrell PJ

更新日期：1993-02-01 00:00:00

abstract：:Viral metagenomics coupled to high-throughput sequencing has provided a powerful tool for large-scale detection of known and unknown viruses associated to distinct hosts and environments. Using this approach, known and novel viruses have been characterized from sylvatic and commercial avian hosts, increasing our under...

journal_title：Virology

authors： Cibulski S,Weber MN,de Sales Lima FE,Lima DA,Fernandes Dos Santos H,Teixeira TF,Varela APM,Tochetto C,Mayer FQ,Roehe PM

更新日期：2020-09-01 00:00:00

abstract：:Why some viruses establish chronic infections while others do not is poorly understood. One possibility is that the host's immune response is impaired during chronic infections and is unable to clear the virus from the host. In this report, we use a recently proposed framework to estimate the per capita killing effica...

journal_title：Virology

authors： Ganusov VV,Lukacher AE,Byers AM

更新日期：2010-09-15 00:00:00

abstract：:We have previously demonstrated that the bZIP transcription factor CREB-2, also called ATF-4, trans-activates, in association with the viral protein Tax, the human T-cell leukemia virus type I (HTLV-I) promoter. In this study, we have examined whether CREB-2 acetylation affects transcriptional activation mediated by T...

journal_title：Virology

authors： Gachon F,Devaux C,Mesnard JM

更新日期：2002-08-01 00:00:00