当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Rhesus angiotensin converting enzyme 2 supports entry of severe acute respiratory syndrome coronavirus in Chinese macaques.

Rhesus angiotensin converting enzyme 2 supports entry of severe acute respiratory syndrome coronavirus in Chinese macaques.

Abstract：

:Angiotensin converting enzyme 2 (ACE2) is the receptor that severe acute respiratory syndrome coronavirus (SARS-CoV) utilizes for target cell entry and, therefore, plays an important role in SARS pathogenesis. Since Chinese rhesus (rh) macaques do not usually develop SARS after SARS-CoV infection, it has been suggested that rh-ACE2 probably does not support viral entry efficiently. To determine the role of rh-ACE2 in early lung pathogenesis in vivo, we studied eleven Chinese rhesus monkeys experimentally infected with a pathogenic SARS-CoV(PUMC01) strain. Rh-ACE2 genes were amplified from all animals by reverse transcription polymerase chain reaction, and their function was studied in vitro using a pseudovirus entry assay. Many natural non-synonymous (NS) changes were found in rh-ACE2 genes. Compared to human (hu) ACE2, thirty-eight consensus NS changes were found in rh-ACE2. Since these changes do not interact with the receptor binding domain of SARS-CoV, rh-ACE2 in general is as effective as human homolog in supporting viral entry. Rh-ACE2, however, is more polymorphic than hu-ACE2. Additional sporadic NS substitutions in clone Rh11-7 reduced the level of rh-ACE2 protein expression and did not support viral entry effectively. Further mutagenesis analysis showed that a natural mutation Y217N dramatically alters ACE2 expression and entry efficiency. Moreover, introduction of the Y217N mutation into hu-ACE2 caused the down-regulation of expression and reduced viral entry efficiency. These results indicate that the Y217N mutation plays a role in modulating SARS-CoV infection. Our results provide insights for understanding the role of rh-ACE2 in SARS lung pathogenesis in a non-human primate model.

journal_name

Virology

journal_title

Virology

authors

Chen Y,Liu L,Wei Q,Zhu H,Jiang H,Tu X,Qin C,Chen Z

doi

10.1016/j.virol.2008.08.016

subject

Has Abstract

pub_date

2008-11-10 00:00:00

pages

89-97

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(08)00523-0

journal_volume

381

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • RNA helicase activity of the plant virus movement proteins encoded by the first gene of the triple gene block.

    abstract：:Cell-to-cell and long-distance transport of some plant viruses requires coordinated action of three movement proteins encoded by triple gene block (TGB). The largest of TGB proteins, TGBp1, is a member of the superfamily I of DNA/RNA helicases and possesses a set of conserved helicase sequence motifs necessary for vir...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.1328

    authors： Kalinina NO,Rakitina DV,Solovyev AG,Schiemann J,Morozov SY

    更新日期：2002-05-10 00:00:00

  • Monoclonal antibodies recognizing normal and retrovirus-transformed chicken hematopoietic cells.

    abstract：:The avian hematopoietic system has long been an invaluable model to study the mechanisms of cell growth and differentiation. We have developed six MAbs against either chicken embryonic hematopoietic precursor cells or retrovirus-transformed cells. MAbs Mo1, Mo2, and Mo3 recognized transformation-associated markers exp...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90581-9

    authors： Liu JL,Klein PA,Moscovici MG,Moscovici C

    更新日期：1992-08-01 00:00:00

  • Role of a heterologous retroviral transport element in the development of genetic complementation assay for mouse mammary tumor virus (MMTV) replication.

    abstract：:The mouse mammary tumor virus (MMTV) is a type B retrovirus that is unique from other retroviruses in having multiple "tissue specific" and "hormone inducible" promoters. This unique feature has lead to the increasing interest in studying the biology of MMTV replication with the ultimate goal of developing MMTV based ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.12.027

    authors： Rizvi TA,Ali J,Phillip PS,Ghazawi A,Jayanth P,Mustafa F

    更新日期：2009-03-15 00:00:00

  • The ecology of primate retroviruses - an assessment of 12 years of retroviral studies in the Taï national park area, Côte d׳Ivoire.

    abstract：:The existence and genetic make-up of most primate retroviruses was revealed by studies of bushmeat and fecal samples from unhabituated primate communities. For these, detailed data on intra- and within-species contact rates are generally missing, which makes identification of factors influencing transmission a challen...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.05.012

    authors： Gogarten JF,Akoua-Koffi C,Calvignac-Spencer S,Leendertz SA,Weiss S,Couacy-Hymann E,Koné I,Peeters M,Wittig RM,Boesch C,Hahn BH,Leendertz FH

    更新日期：2014-07-01 00:00:00

  • In vitro strain adaptation of CWD prions by serial protein misfolding cyclic amplification.

    abstract：:We used serial protein misfolding cyclic amplification (sPMCA) to amplify the D10 strain of CWD prions in a linear relationship over two logs of D10 dilutions. The resultant PMCA-amplified D10 induced terminal TSE disease in CWD-susceptible Tg(cerPrP)1536 mice with a survival time approximately 80 days shorter than th...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.09.023

    authors： Meyerett C,Michel B,Pulford B,Spraker TR,Nichols TA,Johnson T,Kurt T,Hoover EA,Telling GC,Zabel MD

    更新日期：2008-12-20 00:00:00

  • Association of viral load with serum biomakers among COVID-19 cases.

    abstract：:Since SARS-CoV-2 spreads rapidly around the world, data have been needed on the natural fluctuation of viral load and clinical indicators associated with it. We measured and compared viral loads of SARS-CoV-2 from pharyngeal swab, IgM anti-SARS-CoV-2, CRP and SAA from serum of 114 COVID-19 patients on admission. Posit...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2020.04.011

    authors： Shi F,Wu T,Zhu X,Ge Y,Zeng X,Chi Y,Du X,Zhu L,Zhu F,Zhu B,Cui L,Wu B

    更新日期：2020-07-01 00:00:00

  • Characterization of the Rep78/adeno-associated virus complex.

    abstract：:Adeno-associated virus (AAV) replication proteins Rep78 and Rep68 play major roles in the life cycle of AAV. We have recently provided in vivo evidence for the existence of a covalent association between Rep78 and virion single-stranded (ss) AAV DNA (K. M. R. Prasad and J.P. Trempe(1995) Virology 214, 360-370). In thi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.8431

    authors： Prasad KM,Zhou C,Trempe JP

    更新日期：1997-03-03 00:00:00

  • Homologous and heterologous complementation of HBV and WHV capsid and polymerase functions in RNA encapsidation.

    abstract：:Successful encapsidation of hepadnaviral pregenomic RNA requires the orchestrated interaction of the viral capsid and polymerase proteins with each other and with the RNA packaging substrate. The early steps of this process involve binding of the polymerase to the encapsidation signal, epsilon, and are already underst...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0260

    authors： Ziermann R,Ganem D

    更新日期：1996-05-15 00:00:00

  • The porcine lymphotropic herpesvirus 1 encodes functional regulators of gene expression.

    abstract：:The porcine lymphotropic herpesviruses (PLHV) are discussed as possible risk factors in xenotransplantation because of the high prevalence of PLHV-1, PLHV-2 and PLHV-3 in pig populations world-wide and the fact that PLHV-1 has been found to be associated with porcine post-transplant lymphoproliferative disease. To pro...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.08.008

    authors： Lindner I,Ehlers B,Noack S,Dural G,Yasmum N,Bauer C,Goltz M

    更新日期：2007-01-20 00:00:00

  • Characterization of antigenic determinants in the core antigen of the hepatitis C virus.

    abstract：:Antibodies to the hepatitis C virus (HCV) core protein are present in the majority of patients with chronic HCV infection. To characterize the corresponding determinants, synthetic peptides and various deletion clones of the core gene expressed in Escherichia coli were used to test human anti-core positive sera or rab...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1666

    authors： Goeser T,Müller HM,Ye J,Pfaff E,Theilmann L

    更新日期：1994-12-01 00:00:00

  • Effect of exogenous interferon and an interferon inducer on western equine encephalitis virus disease in a hamster model.

    abstract：:Mice are used as models for western equine encephalitis virus (WEEV) infection, but high mortality is generally only seen with intracranial or intranasal challenge, while peripheral inoculation results in approximately 50% mortality and is not dose-dependent. Hamsters were therefore studied as a model for WEEV infecti...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.10.031

    authors： Julander JG,Siddharthan V,Blatt LM,Schafer K,Sidwell RW,Morrey JD

    更新日期：2007-04-10 00:00:00

  • Vaccinia virions deficient in transcription enzymes lack a nucleocapsid.

    abstract：:The poxvirus virion contains an inner tubular nucleocapsid structure. The nucleocapsid is apparently labile to conventional electron microscopy fixation procedures and has therefore been largely ignored for decades. Advancements in electron microscopy sample preparation, notably high pressure freezing, better preserve...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.08.019

    authors： McFadden BD,Moussatche N,Kelley K,Kang BH,Condit RC

    更新日期：2012-12-05 00:00:00

  • A human T-cell line resistant to cytopathic effects of the human immunodeficiency virus (HIV).

    abstract：:Infection of human helper T lymphocytes with the human immunodeficiency virus (HIV) results in a rapid induction of cytopathic effects and cell lysis. We isolated a variant of the human T-lymphoblastoid cell line, CEM, that is fully susceptible to HIV infection but resistant to virally induced cytopathic effects. Expo...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90434-x

    authors： Casareale D,Stevenson M,Sakai K,Volsky DJ

    更新日期：1987-01-01 00:00:00

  • Specific functions of the Rep and Rep׳ proteins of porcine circovirus during copy-release and rolling-circle DNA replication.

    abstract：:The roles of two porcine circovirus replication initiator proteins, Rep and Rep׳, in generating copy-release and rolling-circle DNA replication intermediates were determined. Rep uses the supercoiled closed-circular genome (ccc) to initiate leading-strand synthesis (identical to copy-release replication) and generates...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.01.004

    authors： Cheung AK

    更新日期：2015-07-01 00:00:00

  • The flavivirus 3'-noncoding region: extensive size heterogeneity independent of evolutionary relationships among strains of tick-borne encephalitis virus.

    abstract：:The sequences of the 3'-noncoding regions (NCR) of 12 strains of tick-borne encephalitis (TBE) virus were analyzed and found to vary in length from 350 to approximately 750 nucleotides. The size heterogeneity is restricted to a variable region following the stop codon, whereas the most 3'-terminal 350 nucleotides form...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1557

    authors： Wallner G,Mandl CW,Kunz C,Heinz FX

    更新日期：1995-10-20 00:00:00

  • Inducible system in human hepatoma cell lines for hepatitis C virus production.

    abstract：:We cloned the complete complementary DNA of an isolate of the hepatitis C virus, HCV-S1, into a tetracycline-inducible expression vector and stably transfected it into two human hepatoma cell lines, Huh7 and HepG2. Twenty-six Huh7 and two HepG2-positive clones were obtained after preliminary screening. Two Huh7 (SH-7 ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2002.1687

    authors： Lim SP,Soo HM,Tan YH,Brenner S,Horstmann H,MacKenzie JM,Ng ML,Lim SG,Hong Wj

    更新日期：2002-11-10 00:00:00

  • Cross-inhibition to heterologous foot-and-mouth disease virus infection induced by RNA interference targeting the conserved regions of viral genome.

    abstract：:RNA interference (RNAi) is the process by which double-stranded RNA (dsRNA) directs sequence-specific degradation of messenger RNA in animal and plant cells. In mammalian cells, RNAi can be triggered by 21-23 nucleotide duplexes of small interfering RNA (siRNA). Strategies to inhibit RNA virus multiplication based on ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.01.051

    authors： Liu M,Chen W,Ni Z,Yan W,Fei L,Jiao Y,Zhang J,Du Q,Wei X,Chen J,Liu Y,Zheng Z

    更新日期：2005-05-25 00:00:00

  • The induction of apoptosis by SV40 T antigen correlates with c-jun overexpression.

    abstract：:Simian virus (SV40) T antigen shares many characteristics with adenovirus E1A which is known to induce apoptosis. To verify the potential of SV40 T antigen-mediated apoptosis, we stably expressed T antigen in immortalized human epithelial cells (Z172 and HaCaT). We found that SV40 T antigen could directly cause apopto...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9109

    authors： Chen SL,Tsao YP,Chen YL,Huang SJ,Chang JL,Wu SF

    更新日期：1998-05-10 00:00:00

  • Substrate specificity of the NS3 serine proteinase of hepatitis C virus as determined by mutagenesis at the NS3/NS4A junction.

    abstract：:Hepatitis C virus (HCV) encodes a polyprotein that is processed to produce the structural and nonstructural proteins of the virus. Nonstructural protein 3 (NS3) is a serine proteinase that cleaves the polyprotein to release the NS4A, NS4B, NS5A, and NS5B proteins. To characterize the substrate specificity of NS3, we s...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1520

    authors： Leinbach SS,Bhat RA,Xia SM,Hum WT,Stauffer B,Davis AR,Hung PP,Mizutani S

    更新日期：1994-10-01 00:00:00

  • Host-range determinants on the PB2 protein of influenza A viruses control the interaction between the viral polymerase and nucleoprotein in human cells.

    abstract：:The transcription/replication activity of ribonucleoproteins derived from influenza A primary isolates of human (A/Paris/908/97) or avian origin (A/Mallard/Marquenterre/MZ237/83, A/Hong Kong/156/97) was compared upon reconstitution in mammalian or avian cells, using viral-like reporter RNAs synthesized under the contr...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.12.027

    authors： Labadie K,Dos Santos Afonso E,Rameix-Welti MA,van der Werf S,Naffakh N

    更新日期：2007-06-05 00:00:00

  • Evaluation of the zoonotic potential of multiple subgroups of clade 2.3.4.4 influenza A (H5N8) virus.

    abstract：:Clade 2.3.4.4 H5N8 highly pathogenic avian influenza viruses (HPAIVs) have spread worldwide. Phylogenetic analysis identified two genetic groups of the H5N8 HPAIVs in South Korea; group A evolved further into four subgroups. Here, we examined the zoonotic potential, both in vivo and in vitro, of genetically distinct s...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2017.12.037

    authors： Lee YN,Lee EK,Song BM,Heo GB,Woo SH,Cheon SH,Lee YJ

    更新日期：2018-03-01 00:00:00

  • Molecular cloning of a feline leukemia provirus integrated adjacent to the c-myc gene in a feline T-cell leukemia cell line and the unique structure of its long terminal repeat.

    abstract：:This paper reports the molecular cloning of a rearranged c-myc region from the FT-1 cell line, which was derived from a spontaneous feline T-cell leukemia carrying the feline leukemia virus (FeLV). An abnormal c-myc EcoRI fragment of about 18 kilobases, detected by Southern blotting, was molecularly cloned from the DN...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90172-4

    authors： Miura T,Shibuya M,Tsujimoto H,Fukasawa M,Hayami M

    更新日期：1989-04-01 00:00:00

  • A critical Sp1 element in the rhesus rhadinovirus (RRV) Rta promoter confers high-level activity that correlates with cellular permissivity for viral replication.

    abstract：:KSHV establishes characteristic latent infections in vitro, while RRV, a related macaque rhadinovirus, establishes characteristic permissive infections with virus replication. We identified cells that are not permissive for RRV replication and recapitulate the latent KSHV infection and reactivation processes. The RRV ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2013.10.013

    authors： DeMaster LK,Rose TM

    更新日期：2014-01-05 00:00:00

  • Cellular Hsp27 interacts with classical swine fever virus NS5A protein and negatively regulates viral replication by the NF-κB signaling pathway.

    abstract：:Classical swine fever virus (CSFV) nonstructural protein NS5A is a multifunctional protein functioning in regulation of viral genome replication, protein translation and assembly by interaction with viral or host proteins. Here, heat shock protein 27 (Hsp27) has been identified as a novel binding partner of NS5A by us...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2018.02.020

    authors： Ling S,Luo M,Jiang S,Liu J,Ding C,Zhang Q,Guo H,Gong W,Tu C,Sun J

    更新日期：2018-05-01 00:00:00

  • Antigenic, sequence, and crystal variation in influenza B neuraminidase.

    abstract：:The neuraminidase (NA) genes of influenza B viruses B/Maryland/59, B/Hong Kong/8/73, B/Singapore/222/79, B/Oregon/5/80, B/USSR/100/83, B/Victoria/3/85, B/Leningrad/179/86, B/Memphis/6/86, and B/Memphis/3/89 have been sequenced. The deduced amino acid sequences show high variability in the stalk domain of the NA, but a...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90523-t

    authors： Air GM,Laver WG,Luo M,Stray SJ,Legrone G,Webster RG

    更新日期：1990-08-01 00:00:00

  • Feline leukemia virus variants in experimentally induced thymic lymphosarcomas.

    abstract：:This study was initiated to evaluate the in vivo infectivity and pathogenicity of a group of recombinant feline leukemia viruses (rFeLVs) previously generated by in vitro forced recombination between a FeLV subgroup A virus (FeLV-A) and an endogenous FeLV (enFeLV) envelope (env) element (Sheets et al., 1992, Virology ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.0069

    authors： Pandey R,Bechtel MK,Su Y,Ghosh AK,Hayes KA,Mathes LE,Roy-Burman P

    更新日期：1995-12-20 00:00:00

  • Are the acid-labile interferon alpha and interferon omega-1 identical?

    abstract：:The interferon (IFN) activity found in human leukocyte IFN alpha preparations, autoimmune and AIDS sera, and others was reported to have distinct antigenic and deviating biological properties. This led to its vague designation as acid-labile and thermolabile IFN alpha. However, using specific monoclonal antibodies, th...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90517-f

    authors： Kontsek P,Borecký L,Novák M

    更新日期：1991-03-01 00:00:00

  • Nucleotide sequence of the leader and nucleocapsid protein gene of mumps virus and epitope mapping with the in vitro expressed nucleocapsid protein.

    abstract：:The nucleotide sequence of the leader and the gene encoding the nucleocapsid protein (NP) of mumps virus Miyahara strain have been determined. The leader sequence is 55 nucleotides in length and the NP gene is 1845 nucleotides in length, exclusive of poly(A). The NP gene codes for a protein of 549 amino acids, with a ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90466-5

    authors： Tanabayashi K,Takeuchi K,Hishiyama M,Yamada A,Tsurudome M,Ito Y,Sugiura A

    更新日期：1990-07-01 00:00:00

  • Differential accumulation of genetic and phenotypic changes in Venezuelan equine encephalitis virus and Japanese encephalitis virus following passage in vitro and in vivo.

    abstract：:The requirement to replicate in both vertebrate and invertebrate hosts is thought to limit the introduction of genetic changes into the genome of arboviruses. Serial passage under laboratory conditions will overcome this limitation allowing for genetic changes to be introduced and affecting the virulence of the virus ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.03.030

    authors： McCurdy K,Joyce J,Hamilton S,Nevins C,Sosna W,Puricelli K,Rayner JO

    更新日期：2011-06-20 00:00:00

  • Vaccinia virus gene D12L encodes the small subunit of the viral mRNA capping enzyme.

    abstract：:Vaccinia virus gene D12L, which lies between nucleotides 14,350 and 13,487 in the HindIII D fragment, is transcribed at early times in infection and is capable of encoding a protein 287 amino acids in length with a predicted molecular mass of 33,331. A polyclonal antiserum was raised in rabbits to a fusion protein con...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90194-3

    authors： Niles EG,Lee-Chen GJ,Shuman S,Moss B,Broyles SS

    更新日期：1989-10-01 00:00:00