Abstract:
:The hepatitis B virus core open reading frame with and without the precore domain was expressed in insect cells using a baculovirus expression system. Precore antigen was not properly processed in insect cells and was present in highly insoluble cytoplasmic aggregates. Core antigen without the precore domain formed core particles with a diameter of 28 nm that were secreted into the medium. Both core and precore antigens were phosphorylated in insect cells. The immune response in mice to both antigens yielded antibodies with a high degree of preferential reactivity for the homologous immunizing polypeptide. A kinase activity that phosphorylated core antigen was associated with highly purified core particles. The kinase activity resembled that previously demonstrated for core particles purified from the cytoplasm of infected hepatocytes and detergent-treated Dane particles. Partial resistance of the phosphate-label to phosphatase treatment suggested that some of the phosphorylated sites are in the interior of the particle. The presence of kinase activity in recombinant core particles demonstrated that this activity is not derived from another hepatitis B virus-encoded polypeptide, and the lack of a kinase consensus sequence in the core open reading frame suggests that the kinase is of cellular origin.
journal_name
Virologyjournal_title
Virologyauthors
Lanford RE,Notvall Ldoi
10.1016/0042-6822(90)90247-osubject
Has Abstractpub_date
1990-05-01 00:00:00pages
222-33issue
1eissn
0042-6822issn
1096-0341journal_volume
176pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::It has recently been shown that RNA from the bottom component of cowpea mosaic virus is capable of self-replication in cowpea mesophyll protoplasts, providing a tool for the investigation of the contribution of each RNA strand toward viral multiplication. Evidence is presented here, using this system, to show that the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90239-1
更新日期:1980-10-15 00:00:00
abstract::The expression of cytokines may influence the development of lymphoma in retrovirally infected animals in at least two ways: (1) cytokines in the tumor environment may stimulate the proliferation of tumor cells and/or (2) cytokines in the tumor environment may diminish the cell-mediated antitumor immune response. To e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9846
更新日期:1999-09-01 00:00:00
abstract::Two noncomplementing vaccinia virus temperature-sensitive mutants, Cts8 and Cts26, were mapped to the A3L gene, which encodes the major virion structural protein, 4b. The two ts mutants display normal patterns of gene expression, DNA replication, telomere resolution, and protein processing during infection. Morphogene...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.08.038
更新日期:2004-12-05 00:00:00
abstract::Human papillomaviruses (HPVs) are epitheliotropic viruses, and their life cycle is intimately linked to the stratification and differentiation state of the host epithelial tissues. Defining a role for the E5 gene product in the differentiation-dependent viral life cycle has been difficult due to the lack of a suitable...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9262
更新日期:1998-09-01 00:00:00
abstract::HIV infects macrophages and microglia in the central nervous system (CNS). Mechanisms that enhance HIV macrophage/microglial tropism are not well understood. Here, we identify an HIV Env variant in the V4 region of gp120, Asp 386 (D386), that eliminates an N-linked glycosylation site at position 386, enhances viral re...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.05.029
更新日期:2007-10-10 00:00:00
abstract::To determine if West Nile virus (WNV) infection of insect cells induces a protective RNAi response, Drosophila melanogaster S2 and Aedes albopictus C6/36 cells were infected with WNV, and the production of WNV-homologous small RNAs was assayed as an indicator of RNAi induction. A distinct population of approximately 2...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.04.021
更新日期:2008-07-20 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) establishes lifelong latent infection in sensory neurons of the peripheral nervous system. During HSV latency, the latency-associated transcripts (LATs) are the only viral transcripts abundantly expressed. The most abundant form of LATs is a 2-kb stable intron spliced from a primary...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.03.043
更新日期:2004-07-01 00:00:00
abstract::Recent reports indicated that vaccinia virus late mRNAs contain a unique 5' poly(A) leader sequence and that the in vitro translation of these mRNAs may be relatively cap-independent. These observations led us to examine the possibility that the mechanism of inhibition of host protein synthesis by vaccinia virus, like...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90556-5
更新日期:1992-06-01 00:00:00
abstract::The neuraminidase (NA) genes of influenza B viruses B/Maryland/59, B/Hong Kong/8/73, B/Singapore/222/79, B/Oregon/5/80, B/USSR/100/83, B/Victoria/3/85, B/Leningrad/179/86, B/Memphis/6/86, and B/Memphis/3/89 have been sequenced. The deduced amino acid sequences show high variability in the stalk domain of the NA, but a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90523-t
更新日期:1990-08-01 00:00:00
abstract::Feline calicivirus depends on host-cell proteins for its replication. We previously showed that knockdown of nucleolin (NCL), a phosphoprotein involved in ribosome biogenesis, resulted in the reduction of FCV protein synthesis and virus yield. Here, we found that NCL may not be involved in FCV binding and entry into c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.12.001
更新日期:2016-02-01 00:00:00
abstract::The nucleic acid of carnation cryptic virus has been identified as double-stranded RNA on the basis of (i) susceptibility to RNase in 0.1 x SSC (SSC = 0.15 M NaCl-0.015 M sodium citrate buffer, pH 7.0) and resistance to RNase in 2 x SSC and to DNase, (ii) reaction with an antiserum containing antibodies to dsRNA, and ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90124-0
更新日期:1981-12-01 00:00:00
abstract::A dodecapeptide containing the sequence of the C terminus of the nonstructural polyprotein of Sindbis virus has been synthesized and used to immunize rabbits. The antisera obtained precipitated polypeptides from cells infected with the HR strains of Sindbis or with temperature-sensitive mutants ts11 or ts18. Four diff...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90254-5
更新日期:1985-03-01 00:00:00
abstract::We have proposed that chronic infection of keratinocytes by HPV modifies the expression of potentially important cytokines by interfering with the NF-kappaB signal pathway. We evaluated the constitutive and IL-1beta-induced expression of GM-CSF and TNF-alpha and the expression/activity of NF-kappaB in HPV+ and HPV- ce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1468
更新日期:2002-07-05 00:00:00
abstract::The measles virus (MV) phosphoprotein (P) gene encodes three proteins, P, C, and V. The V protein is synthesized by pseudo-templated transcription, also designated as RNA editing: during P gene transcription one G residue is inserted at a defined position in about 50% of the mRNAs. To study the importance of sequence ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8339
更新日期:1997-01-20 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus origin-dependent DNA replication requires the core replication proteins plus K-Rta and K-bZIP. To determine which K-bZIP protein domains contribute to oriLyt-dependent DNA replication and facilitate suppression of K-Rta-mediated transcriptional activation, we generated a series ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.08.019
更新日期:2007-12-20 00:00:00
abstract::The budded form of Autographa californica nuclear polyhedrosis virus functionally enters cells by adsorptive endocytosis. During the period of virus uptake into Spodoptera frugiperda IPLB Sf21 cells (1 to 4 hr postinfection), filamentous F-actin cables, visualized by fluorescence microscopy, were formed within the cyt...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1585
更新日期:1993-11-01 00:00:00
abstract::Infection with bovine papillomavirus type 1 (BPV1) or BPV2 induces fibropapillomas in cows and skin sarcoids in horses. Prophylactic vaccination targeting BPV1 and BPV2 may reduce the incidence of these economically important diseases. The L1 major capsid proteins of BPV1 and BPV2 were expressed in Sf-9 insect cells a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.036
更新日期:2009-10-10 00:00:00
abstract::NS35, a basic protein encoded by gene 8 of SA11 rotavirus, possesses RNA-binding activity and is essential for genome replication. To identify conserved regions in the NS35 gene and its protein product, we determined the nucleotide sequences of the NS35 gene for the mammalian and avian rotaviruses Wa, DS1, SA11 (Patto...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1059
更新日期:1993-02-01 00:00:00
abstract::Langat (LGT) flavivirus, derived from infectious full-length cDNA clone 636, was investigated for its apoptotic activities in mouse neuroblastoma (Neuro-2a) and simian kidney (Vero and LLC-MK(2)) cells. The hallmark of apoptosis, cleavage of cellular DNA, was observed 48 h after infection of Vero, LLC-MK(2), and Neuro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0980
更新日期:2001-08-01 00:00:00
abstract::Analysis of expression of the Friend murine leukemia virus (F-MuLV) and of the spleen focus forming virus (SFFV) has been undertaken in highly malignant interferon (IFN)-sensitive (745) and IFN-resistant (3Cl-8) Friend leukemia cells (FLC), serially passaged intraperitoneally in DBA/2 mice. In vivo passaged 745 cells,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90304-1
更新日期:1986-04-30 00:00:00
abstract::Strong evidence indicates that virions of mammalian reoviruses undergo proteolytic processing by acid-dependent cellular proteinases as an essential step in productive infection. Proteolytic processing takes the form of a series of cleavages of outer-capsid proteins final sigma3 and mu1/mu1C. Previous studies showed a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9434
更新日期:1998-11-25 00:00:00
abstract::The protein p10 that forms the connector of phage phi 29, has been produced in Escherichia coli harboring a plasmid that carried the gene coding for this protein. The connector protein is assembled in a 13.4-S oligomer that has an apparent molecular weight of 460,000, suggesting that it is a dodecamer. The purified ol...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90251-x
更新日期:1985-03-01 00:00:00
abstract::Antibodies to the hepatitis C virus (HCV) core protein are present in the majority of patients with chronic HCV infection. To characterize the corresponding determinants, synthetic peptides and various deletion clones of the core gene expressed in Escherichia coli were used to test human anti-core positive sera or rab...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1666
更新日期:1994-12-01 00:00:00
abstract::Differing and sometimes conflicting data have been reported regarding several aspects of vaccinia virus (VV) entry. To address this, we used a beta-galactosidase reporter virus to monitor virus entry into multiple cell types under varying conditions. Entry into HeLa, B78H1 and L cells was strongly inhibited by heparin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.12.029
更新日期:2009-03-15 00:00:00
abstract::Commelina yellow mottle virus (CoYMV) is the type member of the badnaviruses, a genus of plant pararetroviruses. The N-terminus of the polyprotein encoded by ORF III has limited similarity to known cell-to-cell movement proteins. To test the hypothesis that the N-terminus is required for viral movement, the phenotypes...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8569
更新日期:1997-06-09 00:00:00
abstract::The polycation DEAE-dextran increased the binding of VSV to BHK cells about fourfold over a wide range of VSV concentrations. The same proportion of bound virions was internalized by the cell in the presence or absence of DEAE-dextran. Viral primary RNA production was increased to the equivalent of a 4- to 4.5-fold in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90429-x
更新日期:1984-02-01 00:00:00
abstract::Virulence markers to distinguish high from low virulence bovine viral diarrhea virus genotype 2 isolates have not been previously reported. The objective of this study was to identify virulence markers by evaluating the primary and secondary structures of the 5'-untranslated region of low and high virulence bovine vir...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9350
更新日期:1998-10-10 00:00:00
abstract::A minor core protein, VP4, of bluetongue virus serotype 10 (BTV-10) has been synthesized in insect cells infected with a genetically manipulated recombinant baculovirus. When insect cells were coinfected by this recombinant virus and a recombinant baculovirus expressing the two major core proteins (VP3 and VP7) of the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90600-t
更新日期:1992-08-01 00:00:00
abstract::Alphaviruses are mosquito-borne viruses that cause serious human and animal diseases. Previous studies demonstrated that a determinant within the nsP1/nsP2 cleavage domain of the virulent Sindbis AR86 virus played a key role in regulating adult mouse virulence without adversely affecting viral replication. Additional ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.12.031
更新日期:2010-03-30 00:00:00
abstract::A new strain of wound tumor virus (WTV) has been isolated from a periwinkle plant (Catharanthus roseus) that was among several used as bait plants in a blueberry field. The 12 segments of double-stranded RNA of the viral genome were isolated directly from infected tissue and found to have mobilities through agarose ge...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90568-v
更新日期:1991-12-01 00:00:00