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Effects of mutations in the C-terminal region of NIa protease on cis-cleavage between NIa and NIb.

Abstract：

:Mutational analyses were carried out to investigate whether the nuclear inclusion protein a (NIa) C-terminal amino acids of turnip mosaic potyvirus play any roles in the cis-cleavage between NIa and NIb. The processing rate of the NIa-NIb junction sequence was decreased significantly by either V240D or Q243A mutation while little affected by F226D, V228E, K230E, I232D, or L235D mutation. The mutation of W212S, G213S, or I217D abolishing the cleavage at the NIb-CP or 6K1-cylindrical inclusion protein junction sequence decreased the processing rate to half the level of that of the wild type. Deletion of the C-terminal one (K230), two (S229 and K230), three (S229 to L231), or six amino acids (S229 to D234) as well as the insertion of five glycines between S229 and K230 or between S220 and Q221 did not affect significantly the cleavage while the deletion of 20 amino acids (Q218 to S237) decreased the processing rate to 73% of that of the wild type. These results rule out the possibility that the C-terminal region plays a role as a spacer in right placement of the NIa-NIb junction sequence and demonstrate that the C-terminal 20 amino acids from Q218 to S237 are not crucial for the cis-cleavage of the NIa-NIb junction sequence.

journal_name

Virology

journal_title

Virology

authors

Kim DH,Han JS,Lew J,Kim SS,Kang BH,Hwang DC,Jang DS,Kim W,Song BD,Choi KY

doi

10.1006/viro.1997.8938

subject

Has Abstract

pub_date

1998-02-01 00:00:00

pages

94-100

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(97)98938-8

journal_volume

241

pub_type

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