Transforming potential of deletion mutants of the SV40 T antigen coding gene in Syrian hamster cells.

Abstract:

:Thymidine kinase-deficient syrian hamster cells were cotransfected with recombinant plasmids containing the thymidine kinase (TK) gene of Herpes Simplex Virus Type 1, and either intact or partially deleted SV40 T antigen-coding genes. The transformants were selected by their ability to grow in gHAT medium. After selection and cloning, the TK-positive transformants that also expressed T antigen were tested for the extent of their transformation with respect to a number of characteristics, which included saturation density, ability to grow in soft agar, resistance to butyrate and to dibutyryl-cAMP, and plating efficiency. The combined results of these various tests indicate that cells containing partially deleted SV40 T antigen-coding genes are less transformed than cells containing an intact SV40 T antigen-coding gene. However, the amounts of T antigen are lower in cells transformed by deletion mutants than in cells transformed by wild-type T antigen-coding gene. Our data indicate that both the quantity and the quality of T antigen may be important in determining the degree of transformation in Syrian hamster cells.

journal_name

Virology

journal_title

Virology

authors

Hirschhorn RR,Mercer WE,Liu HT,Baserga R

doi

10.1016/0042-6822(84)90287-3

subject

Has Abstract

pub_date

1984-04-15 00:00:00

pages

220-9

issue

1

eissn

0042-6822

issn

1096-0341

journal_volume

134

pub_type

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