当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Cucumber mosaic virus-induced RNA replicase: solubilization and partial purification of the particulate enzyme.

Cucumber mosaic virus-induced RNA replicase: solubilization and partial purification of the particulate enzyme.

Abstract：

:Cucumber seedlings infected with cucumber mosaic virus (CMV) contain a virus-induced RNA-dependent RNA polymerase (RNA replicase) in both soluble and particulate fractions of plant extracts; no such activity is found in uninfected plants. The extensive purification and characterization of the soluble RNA replicase has been described (R. Kumarasamy and R. H. Symons, 1979, Virology 96, 622-632) while the characterization of the particulate enzyme is reported here. The particulate RNA replicase was solubilized-by incubation in 150 mM MgS04 at 37 degrees for 5 min; both magnesium and sulfate ions were required for maximum release. Only 5-7% of the protein of the particulate fraction was released under these conditions either in the presence or absence of a variety of salts. The solubilized enzyme was purified about 60-fold by a three-step procedure to a specific activity similar to that of the 10,000-fold purified soluble enzyme. CMV RNA and poly(C)-copying activities copurified through the three steps. Purification was followed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate of the in vitro 3H-labeled proteins. The most prominent virus-induced components were two proteins of Mr 100,000 and 110,000 which correspond in size to two virus-induced proteins in the extensively purified soluble RNA replicase. The Mr 110,000 protein comigrated with the full length in vitro translation product of CMV RNA 2 while the Mr 100,000 was detectably larger than the full length in vitro translation product of CMV RNA 1.

journal_name

Virology

journal_title

Virology

authors

Gill DS,Kumarasamy R,Symons RH

doi

10.1016/0042-6822(81)90131-8

subject

Has Abstract

pub_date

1981-08-01 00:00:00

pages

1-8

issue

1

eissn

0042-6822

issn

1096-0341

pii

0042-6822(81)90131-8

journal_volume

113

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Studies on the attenuation phenotype of polio vaccines: poliovirus RNA polymerase derived from Sabin type 1 sequence is temperature sensitive in the uridylylation of VPg.

    abstract：:Determinants of temperature sensitivity and/or attenuation in Sabin type 1 poliovirus reside in the 5' NTR and coding sequences of the capsid proteins and viral RNA polymerase, 3D(pol). Previous studies have implicated at least two mutations in 3D(pol) of Sabin 1 vaccine strain [PV1(S)], including a Y73H change, as co...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0354

    authors： Paul AV,Mugavero J,Yin J,Hobson S,Schultz S,van Boom JH,Wimmer E

    更新日期：2000-06-20 00:00:00

  • Tetherin restricts HSV-2 release and is counteracted by multiple viral glycoproteins.

    abstract：:Tetherin has been defined as a restriction factor of HIV-1 and several other enveloped viruses. However, the significance of tetherin in viral infection remains to be further addressed. Here, we investigated whether tetherin plays a role in HSV-2 infection. Our study revealed that overexpression of tetherin restricted...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.11.005

    authors： Liu Y,Luo S,He S,Zhang M,Wang P,Li C,Huang W,Hu B,Griffin GE,Shattock RJ,Hu Q

    更新日期：2015-01-15 00:00:00

  • Functional dissection of the ie2 gene product of the baculovirus Autographa californica nuclear polyhedrosis virus.

    abstract：:The PstI-N genomic fragment of Autographa californica nuclear polyhedrosis virus (AcNPV) encodes an immediate-early protein, IE2, that functions as a promiscuous transactivator of other early viral promoters and heterologous promoters both in vivo and in vitro. IE2 contains several sequence motifs that are common to t...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1332

    authors： Yoo S,Guarino LA

    更新日期：1994-07-01 00:00:00

  • An EBNA-negative, EBV-genome-positive human lymphoblast cell line in which superinfecting EBV DNA is not maintained.

    abstract：:A human B-lymphoid cell line, designated TG8, which does not express detectable levels of the EBV (Epstein-Barr virus)-associated nuclear antigen (EBNA), yet carries an average of one to two plasmid copies of the P3HR-1 EBV genome has been identified. TG8 can be superinfected by B95-8 EBV, resulting in up to 60-70% of...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(84)90014-x

    authors： Reisman D,Sugden B

    更新日期：1984-08-01 00:00:00

  • The eIF4AIII RNA helicase is a critical determinant of human cytomegalovirus replication.

    abstract：:Human cytomegalovirus (HCMV) was recently shown to encode a large number of spliced mRNAs. While the nuclear export of unspliced viral transcripts has been extensively studied, the role of host mRNA export factors in HCMV mRNA trafficking remains poorly defined. We found that the eIF4AIII RNA helicase, a component of ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.12.009

    authors： Ziehr B,Lenarcic E,Cecil C,Moorman NJ

    更新日期：2016-02-01 00:00:00

  • A nucleotide sequence rearrangement distinguishes two isolates of satellite tobacco ringspot virus RNA.

    abstract：:Several strains of tobacco ringspot virus (TobRV) support the replication and encapsidation of satellite tobacco ringspot virus RNA (STobRV RNA). We have compared the nucleotide sequences of four STobRV RNAs, each initially associated with a different isolate of TobRV. A STobRV RNA from a geranium isolate of TobRV and...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90049-3

    authors： Buzayan JM,McNinch JS,Schneider IR,Bruening G

    更新日期：1987-09-01 00:00:00

  • Interaction of human immunodeficiency virus type 1 Vpr with the HHR23A DNA repair protein does not correlate with multiple biological functions of Vpr.

    abstract：:The virion-associated Vpr protein of human immunodeficiency virus type 1 (HIV-1) alters cell cycle progression from the G2 phase, influences the virus in vivo mutation rate, and participates in the nuclear translocation of viral DNA. While many Vpr-interacting proteins have been identified, the functional relevance of...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0791

    authors： Mansky LM,Preveral S,Le Rouzic E,Bernard LC,Selig L,Depienne C,Benarous R,Benichou S

    更新日期：2001-03-30 00:00:00

  • HIV-1 Vpu's lipid raft association is dispensable for counteraction of the particle release restriction imposed by CD317/Tetherin.

    abstract：:HIV-1 Vpu antagonizes the block to particle release mediated by CD317 (BST-2/HM1.24/Tetherin) via incompletely understood mechanisms. Vpu and CD317 partially reside in cholesterol-rich lipid rafts where HIV-1 budding preferentially occurs. Here we find that lipid raft association of ectopically expressed or endogenous...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.12.008

    authors： Fritz JV,Tibroni N,Keppler OT,Fackler OT

    更新日期：2012-03-01 00:00:00

  • Scavenger receptor-mediated Ad5 entry and acLDL accumulation in monocytes/macrophages synergistically trigger innate responses against viral infection.

    abstract：:Adenovirus serotype 5 (Ad5) is a common cause of respiratory tract infection, and populations worldwide have high prevalence of anti-Ad5 antibodies, implying extensively prior infection. Ad5 infection potently activates the host innate defense and inflammation, but the molecular mechanisms are not completely clarified...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2018.04.005

    authors： Li P,Feng F,Pan E,Fan X,Yang Q,Guan M,Chen L,Sun C

    更新日期：2018-06-01 00:00:00

  • Preclinical evaluation of cellular immune responses elicited by a polyvalent DNA prime/protein boost HIV-1 vaccine.

    abstract：:While DNA vaccines have been shown to prime cellular immune responses, levels are often low in nonhuman primates or humans. Hence, efforts have been directed toward boosting responses by combining DNA with different vaccination modalities. To this end, a polyvalent DNA prime/protein boost vaccine, consisting of codon ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.10.038

    authors： Cristillo AD,Wang S,Caskey MS,Unangst T,Hocker L,He L,Hudacik L,Whitney S,Keen T,Chou TH,Shen S,Joshi S,Kalyanaraman VS,Nair B,Markham P,Lu S,Pal R

    更新日期：2006-03-01 00:00:00

  • HPV-18 immortalization of human keratinocytes.

    abstract：:The oncogenic potential of human papillomavirus type 18 which is found in a significant number of cervical and penile cancer biopsies was tested in primary human keratinocytes derived from neonatal foreskin. Viral DNA and a gene for resistance to neomycin were introduced into these cells by calcium phosphate transfect...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90247-x

    authors： Kaur P,McDougall JK

    更新日期：1989-11-01 00:00:00

  • E1B and E4 oncoproteins of adenovirus antagonize the effect of apoptosis inducing factor.

    abstract：:Adenovirus inundates the productively infected cell with linear, double-stranded DNA and an abundance of single-stranded DNA. The cellular response to this stimulus is antagonized by the adenoviral E1B and E4 early genes. A mutant group C adenovirus that fails to express the E1B-55K and E4orf3 genes is unable to suppr...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.03.010

    authors： Turner RL,Wilkinson JC,Ornelles DA

    更新日期：2014-05-01 00:00:00

  • Structure of the antigen from Fiji disease virus particles eliciting antibodies specific to double-stranded polyribonucleotides.

    abstract：:Antisera with Fiji disease virus (FDV) protein-specific and double-stranded (ds) RNA-specific antibodies were prepared by injecting rabbits with highly purified preparations of FDV cores. Electron microscopic examination of negatively stained core preparations disclosed the presence of icosahedral particles, about 54 ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(79)90495-1

    authors： van der Lubbe JL,Hatta T,Francki RI

    更新日期：1979-06-01 00:00:00

  • Binding of inferred germline precursors of broadly neutralizing HIV-1 antibodies to native-like envelope trimers.

    abstract：:HIV-1 envelope glycoproteins (Env) and Env-based immunogens usually do not interact efficiently with the inferred germline precursors of known broadly neutralizing antibodies (bNAbs). This deficiency may be one reason why Env and Env-based immunogens are not efficient at inducing bNAbs. We evaluated the binding of 15 ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.08.002

    authors： Sliepen K,Medina-Ramírez M,Yasmeen A,Moore JP,Klasse PJ,Sanders RW

    更新日期：2015-12-01 00:00:00

  • Vaccination with live attenuated simian immunodeficiency virus for 21 days protects against superinfection.

    abstract：:The identification of mechanisms that prevent infection with human immunodeficiency virus (HIV) or simian immunodeficiency virus (SIV) would facilitate the development of an effective AIDS vaccine. In time-course experiments, protection against detectable superinfection with homologous wild-type SIV was achieved withi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.09.026

    authors： Stebbings R,Berry N,Stott J,Hull R,Walker B,Lines J,Elsley W,Brown S,Wade-Evans A,Davis G,Cowie J,Sethi M,Almond N

    更新日期：2004-12-05 00:00:00

  • Prediction of functional class of novel viral proteins by a statistical learning method irrespective of sequence similarity.

    abstract：:The function of a substantial percentage of the putative protein-coding open reading frames (ORFs) in viral genomes is unknown. As their sequence is not similar to that of proteins of known function, the function of these ORFs cannot be assigned on the basis of sequence similarity. Methods complement or in combination...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.10.020

    authors： Han LY,Cai CZ,Ji ZL,Chen YZ

    更新日期：2005-01-05 00:00:00

  • The two nonstructural proteins from wheat dwarf virus involved in viral gene expression and replication are retinoblastoma-binding proteins.

    abstract：:Tumor-inducing viruses like simian virus 40 or the human adenovirus produce oncoproteins which interfere with the cellular retinoblastoma (Rb) tumor-suppressor protein to create an appropriate molecular environment in the nucleus for viral transcription and replication. Such a strategy has been considered to be restri...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0256

    authors： Collin S,Fernández-Lobato M,Gooding PS,Mullineaux PM,Fenoll C

    更新日期：1996-05-01 00:00:00

  • Scrambling of the amino acids within the transmembrane domain of Vpu results in a simian-human immunodeficiency virus (SHIVTM) that is less pathogenic for pig-tailed macaques.

    abstract：:Previous studies have shown that the transmembrane (TM) domain of the subtype B Vpu enhances virion release from cells and some studies have shown that this domain may form an oligomeric structure with properties of an ion channel. To date, no studies have been performed to assess the role of this domain in virus path...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.04.038

    authors： Hout DR,Gomez ML,Pacyniak E,Gomez LM,Inbody SH,Mulcahy ER,Culley N,Pinson DM,Powers MF,Wong SW,Stephens EB

    更新日期：2005-08-15 00:00:00

  • Red clover necrotic mosaic virus replication proteins accumulate at the endoplasmic reticulum.

    abstract：:Red clover necrotic mosaic virus (RCNMV) encodes N-terminally overlapping proteins of 27 and 88 kDa (p27 and p88) known to be required for replication. Green fluorescent protein (GFP) fusions were used to visualize the location of p27 and p88 within Nicotiana benthamiana cells. GFP:p27 fusions localized to the endopla...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2003.12.006

    authors： Turner KA,Sit TL,Callaway AS,Allen NS,Lommel SA

    更新日期：2004-03-15 00:00:00

  • The spike but not the hemagglutinin/esterase protein of bovine coronavirus is necessary and sufficient for viral infection.

    abstract：:The spike (S) and hemagglutinin/esterase (HE) of bovine coronavirus (BCV) are the two envelope proteins that recognize the same receptor-determinant of 9-O-acetylneuraminic acid on host cells. However, the precise and relative roles of the two proteins in BCV infectivity remain elusive. To unequivocally determine thei...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.1307

    authors： Popova R,Zhang X

    更新日期：2002-03-01 00:00:00

  • Protein S and Gas6 induce efferocytosis of HIV-1-infected cells.

    abstract：:Efferocytosis, the phagocytic clearance of apoptotic cells, can provide host protection against certain types of viruses by mediating phagocytic clearance of infected cells undergoing apoptosis. It is known that HIV-1 induces apoptosis and HIV-1-infected cells are efferocytosed by macrophages, although its molecular m...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2017.12.025

    authors： Chua BA,Ngo JA,Situ K,Ramirez CM,Nakano H,Morizono K

    更新日期：2018-02-01 00:00:00

  • In hepatocytes infected with duck hepatitis B virus, the template for viral RNA synthesis is amplified by an intracellular pathway.

    abstract：:During the productive phase of chronic hepadnaviral infections, virion DNA synthesis occurs in the cytoplasm of the infected hepatocyte, but viral RNA is synthesized in the nucleus, apparently from a covalently closed, circular (CCC) viral DNA. J. Tuttleman, C. Pourcel, and J. Summers (1986a, Cell 47, 451-460) have sh...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90206-7

    authors： Wu TT,Coates L,Aldrich CE,Summers J,Mason WS

    更新日期：1990-03-01 00:00:00

  • Single-cell RNA sequencing reveals cell type-specific HPV expression in hyperplastic skin lesions.

    abstract：:Human Papillomavirus infection is highly prevalent worldwide. While most types of HPV cause benign warts, some high-risk types are known to cause cervical cancer, as well as cancer of the oral cavity and head and neck. Persistent cutaneous HPV infection can be particularly problematic in patients with chronic immunosu...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.08.007

    authors： Devitt K,Hanson SJ,Tuong ZK,McMeniman E,Soyer HP,Frazer IH,Lukowski SW

    更新日期：2019-11-01 00:00:00

  • The OC43 human coronavirus envelope protein is critical for infectious virus production and propagation in neuronal cells and is a determinant of neurovirulence and CNS pathology.

    abstract：:The OC43 strain of human coronavirus (HCoV-OC43) is an ubiquitous respiratory tract pathogen possessing neurotropic capacities. Coronavirus structural envelope (E) protein possesses specific motifs involved in protein-protein interaction or in homo-oligomeric ion channel formation, which are known to play various role...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2017.12.023

    authors： Stodola JK,Dubois G,Le Coupanec A,Desforges M,Talbot PJ

    更新日期：2018-02-01 00:00:00

  • Neuronal IFN signaling is dispensable for the establishment of HSV-1 latency.

    abstract：:IFN responses control acute HSV infection, but their role in regulating HSV latency is poorly understood. To address this we used mice lacking IFN signaling specifically in neural tissues. These mice supported a higher acute viral load in nervous tissue and delayed establishment of latency. While latent HSV-1 genome c...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2016.06.016

    authors： Rosato PC,Katzenell S,Pesola JM,North B,Coen DM,Leib DA

    更新日期：2016-10-01 00:00:00

  • Analysis of mutations in the envelope gene of Moloney murine leukemia virus: separation of infectivity from superinfection resistance.

    abstract：:Six deletion mutations and an insertion were generated in the env gene of cloned copies of Moloney murine leukemia virus DNA. All seven mutants were replication-defective as tested by transformation of NIH/3T3 cells. The mutant DNAs were introduced into NIH/3T3 cells to generate stable producer lines; all released vir...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90983-i

    authors： Granowitz C,Colicelli J,Goff SP

    更新日期：1991-08-01 00:00:00

  • Identification of major antigenic domains of duck hepatitis B virus pre-S protein by peptide scanning.

    abstract：:Neutralization epitopes of duck hepatitis B virus (DHBV) have been previously mapped within the N-terminal portion of the pre-S protein using monoclonal antibodies. However, the immune response of ducks to this region is not well characterized at the amino acid level. To this end, we have immunized adult Pekin ducks w...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1164

    authors： Chassot S,Lambert V,Kay A,Godinot C,Trepo C,Cova L

    更新日期：1994-04-01 00:00:00

  • Adenovirus infection inactivates the translational inhibitors 4E-BP1 and 4E-BP2.

    abstract：:Infection with many viruses results in the selective shutoff of host protein synthesis. A common target for virus interference with host protein synthesis is the cap-binding protein complex, eIF4F. The large subunit of the complex, eIF4G, is cleaved upon picornavirus (except cardiovirus) infection. Infection with aden...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8757

    authors： Gingras AC,Sonenberg N

    更新日期：1997-10-13 00:00:00

  • Herpes simplex virus 1 infection is required to produce ICP27 immunoreactive triplet forms when ribosomal aminoacyl-tRNA translocation is blocked by cycloheximide.

    abstract：:Infected cell protein (ICP) 27 is an essential herpes simplex virus type 1 (HSV-1) phosphoprotein required for optimal viral DNA and early or late gene synthesis. Three slow-migrating immunoreactive species were detected using multiple anti-ICP27 antibodies following HSV-1 infection of HEp-2 and Vero cells in the pres...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.04.011

    authors： Sanfilippo CM,Lombardozzi RC,Chirimuuta FN,Blaho JA

    更新日期：2004-07-01 00:00:00

  • Regulation of a geminivirus coat protein promoter by AL2 protein (TrAP): evidence for activation and derepression mechanisms.

    abstract：:Tomato golden mosaic virus (TGMV) is a bipartite member of the subgroup III Geminiviridae. Like all geminiviruses, TGMV replicates in the nucleus of susceptible cells by rolling circle replication (RCR). Double-stranded replicative form DNA generated during RCR serves as template for the transcription of viral genes b...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8549

    authors： Sunter G,Bisaro DM

    更新日期：1997-06-09 00:00:00