Abstract:
:Feline coronavirus infection can progress to a fatal infectious peritonitis, which is a widespread feline disease without an effective vaccine. Generating feline cells with reduced ability to respond to interferon (IFN) is an essential step facilitating isolation of new candidate vaccine strains. Here, we describe the use of Crispr/Cas technology to disrupt type I IFN signaling in two feline cell lines, AK-D and Fcwf-4 CU, and evaluate the replication kinetics of a serotype I feline infectious peritonitis virus (FIPV) within these cells. We report that polyclonal cell populations and a clonal isolate, termed Fcwf-4 IRN, exhibited significantly diminished IFN-responsiveness and allowed FIPV replication kinetics comparable to parental cells. Furthermore, we demonstrate that replication of FIPV is enhanced by ectopic expression of a host serine protease, TMPRSS2, in these cells. We discuss the potential of these cells for isolating new clinical strains and for propagating candidate vaccine strains of FIPV.
journal_name
Virologyjournal_title
Virologyauthors
Mettelman RC,O'Brien A,Whittaker GR,Baker SCdoi
10.1016/j.virol.2019.08.030subject
Has Abstractpub_date
2019-11-01 00:00:00pages
226-236eissn
0042-6822issn
1096-0341pii
S0042-6822(19)30255-7journal_volume
537pub_type
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