Abstract:
:The Rta (R transactivator) protein plays an essential role in the Epstein-Barr viral (EBV) lytic cascade. Rta activates viral gene expression by several mechanisms including direct and indirect binding to target viral promoters, synergy with EBV ZEBRA protein, and stimulation of cellular signaling pathways. We previously found that Rta proteins with C-terminal truncations of 30 aa were markedly enhanced in their capacity to bind DNA (Chen, L.W., Chang, P.J., Delecluse, H.J., and Miller, G., (2005). Marked variation in response of consensus binding elements for the Rta protein of Epstein-Barr virus. J. Virol. 79(15), 9635-9650.). Here we show that two phenylalanines (F600 and F605) in the C-terminus of Rta play a crucial role in mediating this DNA binding inhibitory function. Amino acids 555 to 605 of Rta constitute a functional DNA binding inhibitory sequence (DBIS) that markedly decreased DNA binding when transferred to a minimal DNA binding domain of Rta (aa 1-350). Alanine substitution mutants, F600A/F605A, abolished activity of the DBIS. F600 and F605 are located in the transcriptional activation domain of Rta. Alanine substitutions, F600A/F605A, decreased transcriptional activation by Rta protein, whereas aromatic substitutions, such as F600Y/F605Y or F600W/F605W, partially restored transcriptional activation. Full-length Rta protein with F600A/F605A mutations were enhanced in DNA binding compared to wild-type, whereas Rta proteins with F600Y/F605Y or F600W/F605W substitutions were, like wild-type Rta, relatively poor DNA binders. GAL4 (1-147)/Rta (416-605) fusion proteins with F600A/F605A mutations were diminished in transcriptional activation, relative to GAL4/Rta chimeras without such mutations. The results suggest that, in the context of a larger DBIS, F600 and F605 play a role in the reciprocal regulation of DNA binding and transcriptional activation by Rta. Regulation of DNA binding by Rta is likely to be important in controlling its different modes of action.
journal_name
Virologyjournal_title
Virologyauthors
Chen LW,Raghavan V,Chang PJ,Shedd D,Heston L,Delecluse HJ,Miller Gdoi
10.1016/j.virol.2009.01.022subject
Has Abstractpub_date
2009-04-10 00:00:00pages
448-61issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(09)00047-6journal_volume
386pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The Sindbis virus mutant SB-RL, in contrast to its parent, Sindbis strain AR339 (SB), is attenuated in neonatal mice, has an increased rate of penetration in tissue culture cells, and is more sensitive to neutralization by E2-specific monoclonal antibodies (MCAbs) R6 and R13. These phenotypic differences are controlle...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90184-s
更新日期:1990-03-01 00:00:00
abstract::Encephalitis is a rare, but serious complication from vaccination against smallpox using replication competent strains of vaccinia virus. In this report we describe mutants of vaccinia virus, containing N-terminal deletions of the vaccinia virus interferon resistance gene, E3L, that are attenuated for neuropathogenesi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.01.006
更新日期:2005-03-15 00:00:00
abstract::Vpr is one of the auxiliary gene products encoded by HIV-1 genome. Vpr is a 14-kDa protein and exhibits several interesting characteristics including incorporation into virus particles, oligomerization, localization in the nucleus, and positive regulation of virus replication in primary cells. In an effort to define t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.0079
更新日期:1995-12-20 00:00:00
abstract::The sequence of gene VI from figwort mosaic virus (FMV) clone x4 was determined and compared with that previously published for FMV clone DxS. Both clones originated from the same virus isolation, but the virus used to clone DxS was propagated extensively in a host of a different family prior to cloning whereas that u...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90624-k
更新日期:1991-06-01 00:00:00
abstract::Recent findings suggested that the SUMO-interacting motifs (SIMs) present in the human TRIM5α (TRIM5α(hu)) protein play an important role in the ability of TRIM5α(hu) to restrict N-MLV. Here we explored the role of SIMs in the ability of rhesus TRIM5α (TRIM5α(rh)) to restrict HIV-1, and found that TRIM5α(rh) SIM mutan...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.09.042
更新日期:2013-01-20 00:00:00
abstract::Female BALB/c mice were infected with coxsackievirus B3 in the diestrus, proestrus, estrus, or metestrus phases of the ovarian cycle. Cycle stage was determined by vaginal smear. All mice were killed 7 days after infection. Females infected in the diestrus and especially the proestrus phases developed myocarditis. CD4...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.06.051
更新日期:2004-12-05 00:00:00
abstract::Proviral sequences encoding defective HIV-1 regulatory genes have been detected previously in infected individuals; however, the role of these defective genomes in pathogenesis is unclear. The hypothesis that such replication-defective genomes might induce downregulation of the cellular receptor for HIV-1 (CD4) was te...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1683
更新日期:1994-12-01 00:00:00
abstract::Foot-and-mouth disease virus (FMDV) shows a remarkable antigenic variability. Like other RNA viruses, this virus has a high rate of mutation. It has been proposed that selection exerted by the host's antibodies could play a major role in the rapid evolution of FMDV. The present work reports the selection of FMDV antib...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90629-6
更新日期:1989-08-01 00:00:00
abstract::The heptauridine tract at each gene end and intergenic region (IGR) at the gene junctions of vesicular stomatitis virus (VSV) have effects on synthesis of the downstream mRNA, independent of their respective roles in termination of the upstream mRNA. To investigate the role of the U tract and the IGR in downstream gen...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.08.001
更新日期:2008-10-25 00:00:00
abstract::The infectivity in vivo, replication competence in vitro, and expression of viral genes of several molecularly cloned duck hepatitis B virus (DHBV) genomes were investigated. In addition, replication competence, core protein expression, and secretion of viral proteins were investigated for a grey heron hepatitis B vir...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90782-7
更新日期:1991-11-01 00:00:00
abstract::An Autographa californica nuclear polyhedrosis virus gene encoding an occlusion-derived virus (ODV) envelope protein of 56 kDa was identified and sequenced. Transcription initiates from a conserved baculovirus late motif (ATAAG) with transcripts detected from 16 through 72 hr p.i. The protein is detected in infected c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0097
更新日期:1996-03-01 00:00:00
abstract::The role that interferon-gamma (IFNgamma) plays during herpetic stromal keratitis (HSK) has not been definitively determined. In primary HSK most reports suggest that IFNgamma may help control viral replication and contribute to corneal pathology. However, its role in recurrent HSK has not been directly addressed. The...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.07.018
更新日期:2008-10-10 00:00:00
abstract::Matrix protein M1 purified from influenza A and B viruses has been analyzed for its ability to specifically interact with cellular proteins by immune coprecipitation and by an in vitro binding assay on nitrocellulose on PVDF membranes. When M1 was mixed with lysates of uninfected cells there was selective binding of h...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8700
更新日期:1997-09-01 00:00:00
abstract::Insertion of a functional splicing cassette into a construct containing the HIV-1 poly(A) site followed by the adenovirus L3 poly(A) site results in both specific stimulation of 3' end processing at the HIV-1 site and an increase in the steady-state levels of RNA processed at both sites. To further evaluate this influ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0556
更新日期:1996-10-15 00:00:00
abstract::Binding of a specific peptide(s) from a viral protein to major histocompatibility complex (MHC) class I molecules is a critical step in the activation of CD8(+) cytotoxic T lymphocytes (CTLs). Once activated, CTLs can cause lethal disease in an infected host, for example, by killing virus-containing ependymal and vent...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9593
更新日期:1999-04-10 00:00:00
abstract::Influenza A virus, a respiratory pathogen manipulates various host cellular processes to establish a successful infection in a host. We had reported earlier the interaction of influenza A virus nucleoprotein with host glycolytic enzymes; alpha enolase and pyruvate kinase in A549 cells. Matrix protein (M1), another mul...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.07.019
更新日期:2020-10-01 00:00:00
abstract::Understanding the mechanism of HIV-1 latency is crucial to the viral reservoir eradication. Human cellular miRNAs can modulate HIV-1 expression by targeting of viral RNAs or host gene transcripts. To identify miRNAs modulating HIV-1 latency, we determined the miRNA expression profiles of HIV-1 latently infected and pr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.09.016
更新日期:2015-12-01 00:00:00
abstract::A crucial step in the arenavirus life cycle is the proteolytic processing of the viral envelope glycoprotein precursor (GPC) by the cellular proprotein convertase (PC) subtilisin kexin isozyme-1 (SKI-1)/site-1 protease (S1P). Here we conducted a systematic and quantitative analysis of SKI-1/S1P processing of peptides ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.04.021
更新日期:2011-08-15 00:00:00
abstract::Foot-and-mouth disease virus (FMDV) VP1 G-H loop contains the major antigenic site. By replacing the sequence upstream of the RGD motif with a FLAG epitope, a marker virus for pathogenesis studies was generated. In cell culture, the recombinant virus containing FLAG (A24-FLAG) exhibited similar plaque phenotypes and g...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.11.001
更新日期:2013-02-05 00:00:00
abstract::The sequence of the 1491 nucleotides found at the 3' end of the genome of the highly aphid-transmissible (HAT) isolate of tobacco etch virus (TEV) has been determined. The nucleotide sequence of the capsid protein gene has been identified and compared with the corresponding region of the not-aphid-transmissible (NAT) ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90133-3
更新日期:1985-12-01 00:00:00
abstract::In this study we show that macrophages (Mphi) are latently infected with murine cytomegalovirus (MCMV). After clearance of acute MCMV infection, the predominant form of chronic infection in Balb mice is latency rather than persistence. Peritoneal exudate cells (PECs) from latently infected Balb mice (3-9 months postin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8303
更新日期:1997-01-06 00:00:00
abstract::Although defensins are known to inhibit the replication of human influenza A virus (IAV) in vitro, their in vivo expression during IAV infection is not known. Here we investigated mRNA and protein expression of several beta-defensins in the airways of IAV infected mice. Expression of murine beta-defensin (MBD)-3 and -...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.07.024
更新日期:2008-10-10 00:00:00
abstract::Infectious bronchitis virus (IBV) produces six subgenomic (sg) mRNAs, each containing a 64 nucleotide (nt) leader sequence, derived from the 5' end of the genome by a discontinuous process. Several putative functional domains such as a papain-like proteinase (PL(pro)), main protease (M(pro)), RNA-dependent RNA polymer...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.03.032
更新日期:2004-06-20 00:00:00
abstract::Cytoplasmic tails of envelope (Env) glycoproteins of many retroviruses inhibit their membrane fusion activity. The cytoplasmic 16-amino acid peptide of ecotropic murine leukemia virus (E-MLV) Env protein, called the R-peptide, also inhibits the membrane fusion activity of the Env protein. However, the molecular mechan...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.04.005
更新日期:2019-06-01 00:00:00
abstract::Proviruses were cloned directly from a cat that developed neurological disorders approximately 28 months after inoculation with a molecularly cloned, minimally pathogenic subgroup A feline leukemia virus (FeLV-A). In addition to FeLV-A proviruses that were nearly identical to the inoculated virus, we detected a subgro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1597
更新日期:1994-11-01 00:00:00
abstract::Woodchuck hepatitis core antigen (WHcAg) particles purified from the liver of chronically infected animals were used for monoclonal antibody production. Most of the putative clones demonstrated anti-WHc specificity. However, the supernatants from several putative clones bound X antigen sequences from woodchuck hepatit...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90491-9
更新日期:1990-07-01 00:00:00
abstract::Attempts to define the genetic determinants required for efficient growth of human immunodeficiency virus type 1 (HIV-1) in monocyte-macrophages were made by constructing chimeras between two infectious clones of HIV-1 (HXB2 and LW/C), which despite only minor differences in their DNA sequence have striking difference...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80082-4
更新日期:1995-01-10 00:00:00
abstract::Intact clones containing partial repeats of the genomic components of African cassava mosaic (ACMV DNAs A and B) are infectious when mechanically coinoculated onto Nicotiana benthamiana. Monomeric genomic components may be generated either by homologous recombination or, when two copies of the origin of replication (o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80093-x
更新日期:1995-01-10 00:00:00
abstract::The interferon sensitivity of the expression of an influenza-virus hemagglutinin (HA) gene cloned into the thymidine kinase (TK) gene of vaccinia virus was studied in chick embryo fibroblasts (CEF) and Madin-Darby bovine kidney (MDBK) cells. In CEF, the expression of the HA gene is inhibited by pretreatment of cells w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90740-g
更新日期:1992-05-01 00:00:00
abstract::Endogenous retroviruses (ERVs) arise from the infection and integration of past retroviruses into animal hosts. We performed large-scale genomic mining of 101 avian genomes for discovery of ERVs having none-avian origin and investigated the cross-species transmission events. Phylogenetic analysis of the reverse transc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.02.005
更新日期:2019-04-01 00:00:00