Abstract:
:We have validated a flexible, high-throughput and relatively inexpensive RT-QPCR array platform for absolute quantification of Epstein-Barr virus transcripts in different latent and lytic infection states. Several novel observations are reported. First, during infection of normal B cells, Wp-initiated latent gene transcripts remain far more abundant following activation of the Cp promoter than was hitherto suspected. Second, EBNA1 transcript levels are remarkably low in all forms of latency, typically ranging from 1 to 10 transcripts per cell. EBNA3A, -3B and -3C transcripts are likewise very low in Latency III, typically at levels similar to or less than EBNA1 transcripts. Thirdly, a subset of lytic gene transcripts is detectable in Burkitt lymphoma lines at low levels, including: BILF1, which has oncogenic properties, and the poorly characterized LF1, LF2 and LF3 genes. Analysis of seven African BL biopsies confirmed this transcription profile but additionally revealed significant expression of LMP2 transcripts.
journal_name
Virologyjournal_title
Virologyauthors
Tierney RJ,Shannon-Lowe CD,Fitzsimmons L,Bell AI,Rowe Mdoi
10.1016/j.virol.2014.10.030subject
Has Abstractpub_date
2015-01-01 00:00:00pages
117-30eissn
0042-6822issn
1096-0341pii
S0042-6822(14)00487-5journal_volume
474pub_type
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