Abstract:
:In vitro translation of pH 8-washed particles of tobacco mosaic virus (TMV; vulgare, common, or U1 strain) in a cell-free system derived from Escherichia coli (MRE 600) results in a broad size range of TMV RNA-encoded products, including significant amounts of the distinctive 126-kDa polypeptide which is encoded by the first, 5'-proximal open reading frame in TMV RNA and is conventionally expressed by 80 S eukaryotic ribosomes. In an identical prokaryotic translation system, unencapsidated TMV RNA encodes a series of polypeptides of 50 kDa or less. The predominant product of 16-17 kDa frequently appears to comigrate with TMV coat protein. The significance of the former result for mRNA-ribosome interactions and for possible virus-uncoating/early gene expression events within the chloroplasts of infected cells is discussed.
journal_name
Virologyjournal_title
Virologyauthors
Wilson TMdoi
10.1016/0042-6822(86)90394-6subject
Has Abstractpub_date
1986-07-15 00:00:00pages
277-9issue
1eissn
0042-6822issn
1096-0341pii
0042-6822(86)90394-6journal_volume
152pub_type
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