Nematode transmission of tobacco rattle virus serves as a bottleneck to clear the virus population from defective interfering RNAs.

Abstract:

:DI7 is a defective interfering RNA derived from RNA 2 of tobacco rattle tobravirus (TRV) isolate PpK20. Tobacco was transformed with DI7 cDNA fused to the CaMV 35S promoter. Upon infection of the transgenic plants with TRV isolate PpK20 or the serologically unrelated isolate PaY4, the transgenic DI7 RNA started to accumulate at high levels and strongly interfered with accumulation of wild-type (wt) RNA 2. When DI7 transgenic plants infected with isolate PpK20 were used as source plants in nematode-transmission experiments, the vector Paratrichodorus pachydermus efficiently transmitted virus to healthy bait plants. However, the nematodes transmitted only the wt virus present in the transgenic source plants, whereas virus particles containing the abundant, accumulated DI7 RNA were excluded from transmission. Evidence is presented that wt RNA 2 and DI7 RNA are encapsidated in cis by their encoded CPs, which are known to be functional and nonfunctional in transmission, respectively. This mechanism would result in defective interfering RNAs, which rapidly arise after mechanical transmission of the virus in the laboratory, being eliminated from tobraviruses under natural field conditions. Also this mechanism which acts with nematode transmitted virus isolates contrasts with that of vector-transmission of defective potyviruses and luteoviruses by wt helper viruses.

journal_name

Virology

journal_title

Virology

authors

Visser PB,Brown DJ,Brederode FT,Bol JF

doi

10.1006/viro.1999.9901

subject

Has Abstract

pub_date

1999-10-10 00:00:00

pages

155-65

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(99)99901-4

journal_volume

263

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • A variant of CRPV DNA preferentially maintained as a plasmid in NIH 3T3 cells and characterization of its transcripts in nude mouse tumors.

    abstract::Rabbit and human papillomaviruses are strictly epitheliotropic and their DNA replicates extrachromosomally in benign lesions (warts) of their natural host. Their tissue and host specificity is thought to be genetically controlled and may account for the inefficiency in transforming heterologous cells. In our hands, CR...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(87)90149-8

    authors: Nasseri M,Wettstein FO

    更新日期:1987-12-01 00:00:00

  • ZikaBase: An integrated ZIKV- Human Interactome Map database.

    abstract::Re-emergence of ZIKV has caused infections in more than 1.5 million people. The molecular mechanism and pathogenesis of ZIKV is not well explored due to unavailability of adequate model and lack of publically accessible resources to provide information of ZIKV-Human protein interactome map till today. This study made ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2017.11.007

    authors: Gurumayum S,Brahma R,Naorem LD,Muthaiyan M,Gopal J,Venkatesan A

    更新日期:2018-01-15 00:00:00

  • Differential subcellular localization of hepatitis C virus core gene products.

    abstract::The expression of the core gene of two different hepatitis C virus (HCV) isolates was analyzed. In the presence of its downstream E1 envelope protein sequence, two major core protein products with molecular masses of 21 kDa (P21) and 19 kDa (P19) and a minor protein product with molecular mass of 16 kDa (P16) were det...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1995.0018

    authors: Lo SY,Masiarz F,Hwang SB,Lai MM,Ou JH

    更新日期:1995-11-10 00:00:00

  • Epigenetics of human papillomaviruses.

    abstract::Human papilllomaviruses (HPVs) are common human pathogens that infect cutaneous or mucosal epithelia in which they cause warts, self-contained benign lesions that commonly regress. The HPV life cycle is intricately tied to the differentiation of the host epithelium it infects. Mucosotropic HPVs are the most common sex...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2013.07.016

    authors: Johannsen E,Lambert PF

    更新日期:2013-10-01 00:00:00

  • Structure of phage phi 29 connector protein assembled in vivo.

    abstract::The protein p10 that forms the connector of phage phi 29, has been produced in Escherichia coli harboring a plasmid that carried the gene coding for this protein. The connector protein is assembled in a 13.4-S oligomer that has an apparent molecular weight of 460,000, suggesting that it is a dodecamer. The purified ol...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(85)90251-x

    authors: Carrascosa JL,Carazo JM,Ibañez C,Santisteban A

    更新日期:1985-03-01 00:00:00

  • Comparison of viral replication and IFN response in alpaca and bovine cells following bovine viral diarrhea virus infection.

    abstract::Alpacas develop diminished disease following bovine viral diarrhea virus (BVDV) infection compared to cattle. We hypothesized that alpaca and bovine cells have differential permissiveness and responses to BVDV infection. To characterize alpaca testicular (AT) and bovine turbinate (BT) cells BVDV infection permissivene...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2011.02.006

    authors: Samson HC,Topliff CL,Donis RO,Kelling CL

    更新日期:2011-04-25 00:00:00

  • HIV-1 subtype C in vitro growth and coreceptor utilization.

    abstract::Human immunodeficiency virus type 1 subtype C (HIV-1C) accounts for about 50% of all HIV infections in the pandemic and is the predominant subtype in the heavily burdened region of southern Africa. HIV-1C possesses unique genetic and phenotypic features that might be associated with biological differences compared to ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2005.11.047

    authors: Ndung'u T,Sepako E,McLane MF,Chand F,Bedi K,Gaseitsiwe S,Doualla-Bell F,Peter T,Thior I,Moyo SM,Gilbert PB,Novitsky VA,Essex M

    更新日期:2006-04-10 00:00:00

  • Differential evolution of cell-associated virus in blood and genital tract of HIV-infected females undergoing HAART.

    abstract::PBMC and vaginal cell (VC) viruses were studied from 5 HIV-infected females for the presence of drug-resistance and non-drug resistance associated mutations. A 1318-bp fragment of polymerase gene was amplified from PBMC and VC proviral DNA. Four of the 5 PBMC viruses exhibited drug resistance-associated mutations in r...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2005.01.030

    authors: Tirado G,Jove G,Reyes E,Sepulveda G,Yamamura Y,Singh DP,Kumar A

    更新日期:2005-04-10 00:00:00

  • A reassortant between influenza A viruses (H7N2) synthesizing an enzymatically inactive neuraminidase at 40 degrees which is not incorporated into infectious particles.

    abstract::Cells infected with a reassortant (113/Ho, H7N2) between A/fowl plague/Rostock/34 (FPV, H7N1) and A/Hong Kong/1/68 (H3N2) carrying RNA segments 1 and 6 of the Hong Kong virus and the residual genes of FPV, synthesized at 40 degrees a neuraminidase (NA) which is enzymatically not active and which is not incorporated in...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(86)90266-7

    authors: Breuning A,Scholtissek C

    更新日期:1986-04-15 00:00:00

  • Transforming genes of avian (v-fps) and mammalian (v-fes) retroviruses correspond to a common cellular locus.

    abstract::The Gardner (GA) and Snyder-Theilen (ST) isolates of feline sarcoma virus (FeSV) represent genetic recombinants between feline leukemia virus (FeLV) and transformation-specific sequences (v-fes gene) of cat cellular origin. A related transforming gene (v-fps), common to the Fujinami, PRC II, and UR 1 strains of avian ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(83)90219-2

    authors: Groffen J,Heisterkamp N,Shibuya M,Hanafusa H,Stephenson JR

    更新日期:1983-03-01 00:00:00

  • Development of an in vitro mRNA degradation assay utilizing extracts from HIV-1- and SIV-infected cells.

    abstract::We previously demonstrated that cellular mRNAs are degraded in CD4 positive lymphocytes infected by the human immunodeficiency virus, HIV-1, but not in cells infected by the simian lentivirus, SIV. To begin to define the molecular mechanisms underlying this RNA degradation, we have established an in vitro RNA degradat...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1996.0103

    authors: Agy MB,Sherbert CH,Katze MG

    更新日期:1996-03-01 00:00:00

  • In vitro dissection of the membrane and RNP binding activities of influenza virus M1 protein.

    abstract::Spontaneous proteolysis of influenza virus M1 protein during crystallisation has defined an N-terminal domain of amino acids 1--164. Full-length M1, the N-terminal domain, and the C-terminal part of M1 (residues 165--252) were produced in Escherichia coli. In vitro tests showed that only full-length M1 and its N-termi...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.2000.0804

    authors: Baudin F,Petit I,Weissenhorn W,Ruigrok RW

    更新日期:2001-03-01 00:00:00

  • Structure of the antigen from Fiji disease virus particles eliciting antibodies specific to double-stranded polyribonucleotides.

    abstract::Antisera with Fiji disease virus (FDV) protein-specific and double-stranded (ds) RNA-specific antibodies were prepared by injecting rabbits with highly purified preparations of FDV cores. Electron microscopic examination of negatively stained core preparations disclosed the presence of icosahedral particles, about 54 ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(79)90495-1

    authors: van der Lubbe JL,Hatta T,Francki RI

    更新日期:1979-06-01 00:00:00

  • Autographa californica multiple nucleopolyhedrovirus core gene ac92 (p33) is required for efficient budded virus production.

    abstract::Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac92 is a core gene encoding a protein associated with occlusion derived virus (ODV), binds human P53 and also has flavin adenine dinucleotide linked sulfhydryl oxidase activity but its role in the virus life cycle is not known. To determine ac92 function a...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2010.09.023

    authors: Nie Y,Fang M,Theilmann DA

    更新日期:2011-01-05 00:00:00

  • Restriction of a Sindbis virus mutant in BHK cells and relief of the restriction by the addition of adenosine.

    abstract::SV(PZF) is a mutant of Sindbis virus (SV) which we selected on the basis of its ability to replicate in mosquito cells treated with pyrazofurin (PZF), a drug which inhibits pyrimidine nucleotide biosynthesis (Lin et al., 2000, Virology 272, 61-71). Three mutations, A6627U, A7543U, and C7593A, were identified in the ns...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.2001.1230

    authors: Lin YH,Simmonds HA,Stollar V

    更新日期:2002-01-05 00:00:00

  • Antigenic mapping of the surface proteins of rhesus rotavirus.

    abstract::Monoclonal antibodies have been produced and used to map the functional topography of the surface proteins of rhesus rotavirus (RRV) that mediate viral neutralization. Ten monoclonal antibodies directed to VP7 were studied in neutralization assays and competitive binding studies. A large neutralization domain with sev...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(86)90205-9

    authors: Shaw RD,Vo PT,Offit PA,Coulson BS,Greenberg HB

    更新日期:1986-12-01 00:00:00

  • Nucleotide sequences within the U5 region of the viral RNA genome are the major determinants for an human immunodeficiency virus type 1 to maintain a primer binding site complementary to tRNA(His).

    abstract::The initiation of reverse transcription of the human immunodeficiency virus type 1 (HIV-1) genome requires cellular tRNA(Lys,3) as a primer and occurs at a site in the viral RNA genome, designated as the primer binding site (PBS), which is complementary to the 3'-terminal 18 nucleotides of tRNA(Lys,3). We previously d...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1996.0658

    authors: Zhang Z,Kang SM,LeBlanc A,Hajduk SL,Morrow CD

    更新日期:1996-12-15 00:00:00

  • Molecular and serological characterization of SARS-CoV-2 infection among COVID-19 patients.

    abstract:BACKGROUND:SARS-CoV-2 is a novel coronavirus and the cause of COVID-19. More than 80% of COVID-19 patients exhibit mild or moderate symptoms. In this study, we investigated the dynamics of viral load and antibodies against SARS-CoV-2 in a longitudinal cohort of COVID-19 patients with severe and mild/moderate diseases. ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2020.09.008

    authors: Li L,Liang Y,Hu F,Yan H,Li Y,Xie Z,Huang L,Zhao J,Wan Z,Wang H,Shui J,Cai W,Tang S

    更新日期:2020-12-01 00:00:00

  • Quasispecies, error catastrophe, and the antiviral activity of ribavirin.

    abstract::Ribavirin is the first synthetic, broad-spectrum antiviral nucleoside. Despite its more than 30 year history, the mechanism of action of this compound remains unclear and somewhat controversial. Recent data suggest the possibility that the activity of ribavirin against RNA viruses is a reflection of incorporation of r...

    journal_title:Virology

    pub_type: 杂志文章,评审

    doi:10.1006/viro.2002.1487

    authors: Graci JD,Cameron CE

    更新日期:2002-07-05 00:00:00

  • Transcriptional analysis of porcine circovirus type 2.

    abstract::Porcine circovirus type 2 (PCV2) is the causative agent of an emerging swine disease, postweaning multisystemic wasting syndrome. In this work, the RNAs of PCV2 synthesized during productive infection in porcine kidney cells were characterized. A total of nine RNAs were detected. They include the viral capsid protein ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.2002.1733

    authors: Cheung AK

    更新日期:2003-01-05 00:00:00

  • Primary human cervical carcinoma cells require human papillomavirus E6 and E7 expression for ongoing proliferation.

    abstract::Repression of human papillomavirus (HPV) E6 and E7 oncogenes in established cervical carcinoma cell lines causes senescence due to reactivation of cellular tumor suppressor pathways. Here, we determined whether ongoing expression of HPV16 or HPV18 oncogenes is required for the proliferation of primary human cervical c...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2011.10.012

    authors: Magaldi TG,Almstead LL,Bellone S,Prevatt EG,Santin AD,DiMaio D

    更新日期:2012-01-05 00:00:00

  • Efficient inoculation with CaMV 35 S promoter-driven DNA clones of the tobravirus PEBV.

    abstract::Clones have been constructed containing full-length cDNA copies of PEBV RNA1 and RNA2, flanked by the CaMV 35 S RNA promoter and the nopaline synthase terminator. The clones are infectious when inoculated onto Nicotiana benthamiana plants. Both the viral RNAs and the virus particles were identified in infected plants....

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(92)90488-b

    authors: MacFarlane SA,Gilmer D,Davies JW

    更新日期:1992-04-01 00:00:00

  • The v-Rel oncoprotein complexes with new Rel- and RelA-related proteins in transformed cells.

    abstract::The v-Rel oncoprotein of the Rev-T retrovirus interacts with a number of cellular proteins in transformed chicken spleen cells including p40/I kappa B alpha, p68c-Rel, hsc70, and the p124 and p115 precursors for the p50 and p52 subunits of NF kappa B. Here we report that v-Rel associates with at least three other cell...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1995.1095

    authors: Xu X,Gélinas C

    更新日期:1995-03-10 00:00:00

  • Identification, expression, and processing of an 87-kDa polypeptide encoded by ORF 1a of the coronavirus infectious bronchitis virus.

    abstract::Nucleotide sequence analysis has shown previously that the genomic-length mRNA (mRNA1) of the coronavirus infectious bronchitis virus (IBV) contains two large open reading frames (ORFs), 1a and 1b, with the potential to encode polyproteins of approximately 441 and 300 kDa, respectively. We have characterized the speci...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1995.1128

    authors: Liu DX,Tibbles KW,Cavanagh D,Brown TD,Brierley I

    更新日期:1995-04-01 00:00:00

  • Deletion and duplication mutations in the C-terminal nonconserved region of Sindbis virus nsP3: effects on phosphorylation and on virus replication in vertebrate and invertebrate cells.

    abstract::Little is known concerning the function of the C-terminal nonconserved region of Sindbis virus nsP3. In this report, we created a number of in-frame deletions and duplications (from 12 to 159 residues) in this region and examined their effects on Sindbis virus replication. Sindbis RNA transcripts containing these muta...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1994.1338

    authors: Lastarza MW,Grakoui A,Rice CM

    更新日期:1994-07-01 00:00:00

  • Single-cell RNA sequencing reveals cell type-specific HPV expression in hyperplastic skin lesions.

    abstract::Human Papillomavirus infection is highly prevalent worldwide. While most types of HPV cause benign warts, some high-risk types are known to cause cervical cancer, as well as cancer of the oral cavity and head and neck. Persistent cutaneous HPV infection can be particularly problematic in patients with chronic immunosu...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2019.08.007

    authors: Devitt K,Hanson SJ,Tuong ZK,McMeniman E,Soyer HP,Frazer IH,Lukowski SW

    更新日期:2019-11-01 00:00:00

  • Altered DNA binding and replication activities of JC virus T-antigen mutants.

    abstract::Ten mutations were introduced into the JC virus (JCV) T antigen within a region corresponding to the SV40 T-antigen DNA binding domain (SV40 amino acids 131 to 220); nine of these increased homology between the two proteins in sequences critical for SV40 T antigen DNA binding. All mutant JCV T antigens bound to JCV an...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(91)90136-y

    authors: Tavis JE,Frisque RJ

    更新日期:1991-07-01 00:00:00

  • Transcriptive complex of Newcastle disease virus. II. Structural and functional assembly associated with the cytoskeletal framework.

    abstract::The transcriptive complex, the nucleocapsid with the viral RNA-synthesizing activity, of Newcastle disease virus (NDV) contains three protein components, the major structural subunit (NP) and two associated proteins (P and L) involved in the RNA synthesis. We studied the pathway of these proteins from synthesis to ass...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(85)90132-1

    authors: Hamaguchi M,Nishikawa K,Toyoda T,Yoshida T,Hanaichi T,Nagai Y

    更新日期:1985-12-01 00:00:00

  • Analyses of mutations selected by passaging a chimeric flavivirus identify mutations that alter infectivity and reveal an interaction between the structural proteins and the nonstructural glycoprotein NS1.

    abstract::We previously described a single-cycle dengue vaccine (RepliVAX D2) engineered from a capsid (C) gene-deleted West Nile virus (WNV) expressing dengue virus serotype 2 (DENV2) prM/E genes in place of the corresponding WNV genes. That work demonstrated that adaptation of RepliVAX D2 to grow in WNV C-expressing cells res...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2011.09.007

    authors: Winkelmann ER,Widman DG,Suzuki R,Mason PW

    更新日期:2011-12-20 00:00:00

  • Cryo-EM structures of two bovine adenovirus type 3 intermediates.

    abstract::Adenoviruses (Ads) infect hosts from all vertebrate species and have been investigated as vaccine vectors. We report here near-atomic structures of two bovine Ad type 3 (BAd3) intermediates obtained by cryo-electron microscopy. A comparison between the two intermediate structures reveals that the differences are local...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2013.12.012

    authors: Cheng L,Huang X,Li X,Xiong W,Sun W,Yang C,Zhang K,Wang Y,Liu H,Huang X,Ji G,Sun F,Zheng C,Zhu P

    更新日期:2014-02-01 00:00:00