当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Sequence conservation and divergence of hepatitis delta virus RNA.

Sequence conservation and divergence of hepatitis delta virus RNA.

Abstract：

:The complete RNA sequence of the hepatitis delta virus (HDV) obtained from the Nauru Island in the Pacific was determined by cDNA cloning and amplification by polymerase chain reaction (PCR). The sequence showed 14-17% divergence from the two known HDV RNA sequences. There are three highly conserved domains: the region around the autocatalytic cleavage site of the genomic RNA (nucleotides 659 to 772), the region around the autocatalytic cleavage site of the antigenomic-sense RNA (nucleotides 847 to 966), and the region around the middle one-third domain of the open reading frame (ORF) encoding the hepatitis delta antigen on the antigenomic RNA (nucleotides 1267 to 1347). The two autocatalytic activities are required for the cleavage and ligation of HDV RNA during RNA replication. The third conserved domain codes for the RNA-binding domain of HDAg, which specifically interacts with HDV RNA. Three nucleotide changes within the genomic catalytic sequence are present but did not alter the catalytic cleavage activity of the HDV RNA. Microheterogeneity of the RNA sequences was also detected. One of these occurred within the coding region of the delta antigen, creating an amber termination codon in some of the RNA species. Thus, this HDV strain contains two different RNA species, one of which encodes a delta antigen of 214 amino acids and the other 195 amino acids. These two protein species were detected by immunoblotting of the patient's plasma. In contrast to other HDV strains, only three ORFs capable of encoding more than 100 amino acids each are present in this HDV RNA. We recommend that oligonucleotides complementary to the highly conserved sequences should be used as primers for PCR in clinical detection assays of hepatitis delta virus infection.

journal_name

Virology

journal_title

Virology

authors

Chao YC,Chang MF,Gust I,Lai MM

doi

10.1016/0042-6822(90)90335-o

subject

Has Abstract

pub_date

1990-10-01 00:00:00

pages

384-92

issue

2

eissn

0042-6822

issn

1096-0341

journal_volume

178

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Cowpea mosaic virus-based chimaeras. Effects of inserted peptides on the phenotype, host range, and transmissibility of the modified viruses.

    abstract：:Expression of foreign peptides on the surface of cowpea mosaic virus particles leads to the creation of chimaeras with a variety of phenotypes and yields. Two factors were shown to be particularly significant in determining the properties of a given chimaera: the length of the inserted sequence and its isoelectric poi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00140-5

    authors： Porta C,Spall VE,Findlay KC,Gergerich RC,Farrance CE,Lomonossoff GP

    更新日期：2003-05-25 00:00:00

  • Select resistance-associated mutations in blood are associated with lower CSF viral loads and better neuropsychological performance.

    abstract：BACKGROUND:When antiretroviral therapy does not fully suppress HIV replication, suboptimal levels of antiretrovirals can select for antiretroviral resistant variants of HIV. These variants may exhibit reduced replication capacity and result in lower viral loads in blood. Our study evaluated whether antiretroviral resis...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.08.007

    authors： Hightower GK,Letendre SL,Cherner M,Gibson SA,Ellis RJ,Wolfson TJ,Gamst AC,Ignacio CC,Heaton RK,Grant I,Richman DD,Smith DM,HNRC Group.

    更新日期：2009-11-25 00:00:00

  • Epigenetics of human papillomaviruses.

    abstract：:Human papilllomaviruses (HPVs) are common human pathogens that infect cutaneous or mucosal epithelia in which they cause warts, self-contained benign lesions that commonly regress. The HPV life cycle is intricately tied to the differentiation of the host epithelium it infects. Mucosotropic HPVs are the most common sex...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2013.07.016

    authors： Johannsen E,Lambert PF

    更新日期：2013-10-01 00:00:00

  • Tyrosine sulfation of varicella-zoster virus envelope glycoprotein gpl.

    abstract：:Sulfation is a common post-translational modification of secreted and membrane proteins, with the sulfate attached to tyrosine residues or to glycan side-chains. I have shown that varicella-zoster virus (VZV) envelope glycoproteins gpI, gpII, and gpIII can be labeled with [35S]sulfate. The predominant VZV glycoprotein...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1576

    authors： Edson CM

    更新日期：1993-11-01 00:00:00

  • The measles virus phosphoprotein interacts with the linker domain of STAT1.

    abstract：:The measles virus (MV) phosphoprotein (P) and V proteins block the interferon (IFN) response by impeding phosphorylation of the signal transducer and activator of transcription 1 (STAT1) by the Janus kinase 1 (JAK1). We characterized how STAT1 mutants interact with P and JAK1 phosphorylation. Certain mutants of the li...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2013.06.019

    authors： Devaux P,Priniski L,Cattaneo R

    更新日期：2013-09-01 00:00:00

  • The X protein of hepatitis B virus has a ribo/deoxy ATPase activity.

    abstract：:The X protein (pX) of hepatitis B virus (HBV) is a general transcription regulator and directly associated with the transcription machinery. pX cannot bind DNA directly but interacts with cellular factors that bind the regulatory elements. There is an accumulation of evidence concerning different activities exerted by...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1356

    authors： de-Medina T,Haviv I,Noiman S,Shaul Y

    更新日期：1994-07-01 00:00:00

  • Restriction of Moloney murine leukemia virus replication in Moloney murine sarcoma virus-infected cells.

    abstract：:MuSV349 is a TB-cell line which produces infectious MuSV with little or no MuLV detectable by the XC assay. The apparent restriction of MuLV replication in MuSV349 cells was investigated. A replication-competent helper virus, with protein composition nearly identical to that of Mo-MuLV was isolated from the viruses pr...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(84)90043-6

    authors： Yuen PH,Soong MM,Kissil MS,Wong PK

    更新日期：1984-01-30 00:00:00

  • Analysis of complex mutations induced in cells by herpes simplex virus type-1.

    abstract：:The shuttle vector plasmid pZ189 was used as a target for mutagenesis in COS-1 cells. Complex mutations were analyzed in 5 plasmids that were recovered from noninfected cells and 15 plasmids that were recovered from cells that were infected with herpes simplex virus type-1 (HSV-1). Complex mutations in noninfected cel...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90895-i

    authors： Hwang CB,Shillitoe EJ

    更新日期：1991-04-01 00:00:00

  • Identification and genomic mapping of the ORF3 and VPg proteins in feline calicivirus virions.

    abstract：:Two minor proteins with molecular masses of 8.5 and 15.5 kDa were identified in feline calicivirus (FCV) virions. Direct sequence analysis showed that the N-terminal sequence of the 8.5-kDa protein was identical to that of the predicted protein encoded by open reading frame 3 (ORF3) of the FCV genome. The N-terminal s...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0579

    authors： Sosnovtsev SV,Green KY

    更新日期：2000-11-10 00:00:00

  • TCR triggering transcriptionally downregulates CCR5 expression on rhesus macaque CD4(+) T-cells with no measurable effect on susceptibility to SIV infection.

    abstract：:Studies using transformed human cell lines suggest that most SIV strains use CCR5 as co-receptor. Our analysis of primary rhesus macaque CD4(+) T-cell clones revealed marked differences in susceptibility to SIV(mac)239 infection. We investigated whether different levels of CCR5 expression account for clonal difference...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.10.005

    authors： Minang JT,Trivett MT,Barsov EV,Del Prete GQ,Trubey CM,Thomas JA,Gorelick RJ,Piatak M Jr,Ott DE,Ohlen C

    更新日期：2011-01-05 00:00:00

  • Deletion and duplication mutations in the C-terminal nonconserved region of Sindbis virus nsP3: effects on phosphorylation and on virus replication in vertebrate and invertebrate cells.

    abstract：:Little is known concerning the function of the C-terminal nonconserved region of Sindbis virus nsP3. In this report, we created a number of in-frame deletions and duplications (from 12 to 159 residues) in this region and examined their effects on Sindbis virus replication. Sindbis RNA transcripts containing these muta...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1338

    authors： Lastarza MW,Grakoui A,Rice CM

    更新日期：1994-07-01 00:00:00

  • Role of matrix protein in the type D retrovirus replication cycle: importance of the arginine residue at position 55.

    abstract：:We previously reported that a mutant of Mason-Pfizer monkey virus (M-PMV), which has an amino acid substitution in the matrix (MA) protein at position 55, MA-R55W, showed altered viral morphogenesis, reduced glycoprotein incorporation, and loss of infectivity. In this report, we show that two additional amino acid sub...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.0179

    authors： Yasuda J,Hunter E

    更新日期：2000-03-15 00:00:00

  • Baculovirus virions displaying Plasmodium berghei circumsporozoite protein protect mice against malaria sporozoite infection.

    abstract：:The display of foreign proteins on the surface of baculovirus virions has provided a tool for the analysis of protein-protein interactions and for cell-specific targeting in gene transfer applications. To evaluate the baculovirus display system as a vaccine vehicle, we have generated a recombinant baculovirus (AcNPV-C...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2003.08.003

    authors： Yoshida S,Kondoh D,Arai E,Matsuoka H,Seki C,Tanaka T,Okada M,Ishii A

    更新日期：2003-11-10 00:00:00

  • Potential of ancestral sylvatic dengue-2 viruses to re-emerge.

    abstract：:Dengue viruses (DENV) are the most important arboviral pathogens in tropical and subtropical regions throughout the world. DENV transmission includes both a sylvatic, enzootic cycle between nonhuman primates and arboreal mosquitoes of the genus Aedes, and an urban, endemic/epidemic cycle between Aedes aegypti, a mosqu...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.08.049

    authors： Vasilakis N,Shell EJ,Fokam EB,Mason PW,Hanley KA,Estes DM,Weaver SC

    更新日期：2007-02-20 00:00:00

  • Differential growth of human enteric adenovirus 41 (TAK) in continuous cell lines.

    abstract：:Differing reports exist about the replication of human enteric adenoviruses (EnAds) in various cell lines. There was a suggestion that EnAds are defective, do not grow on primary human diploid cells, and behave like Ad host-range mutants, i.e., they require early gene products from other Ad types for efficient growth....

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90072-y

    authors： Pieniazek D,Pieniazek NJ,Macejak D,Coward J,Rayfield M,Luftig RB

    更新日期：1990-01-01 00:00:00

  • A simple technique for the rescue of early region I mutations into infectious human adenovirus type 5.

    abstract：:Early region 1 (E1) of the human adenoviruses has many intriguing properties which have prompted numerous mutational studies to help delineate and characterize the domains responsible for these functions. In mutational analyses being done currently, the E1 region is usually cloned into a bacterial plasmid where it is ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(88)90302-9

    authors： McGrory WJ,Bautista DS,Graham FL

    更新日期：1988-04-01 00:00:00

  • Protection from SARS coronavirus conferred by live measles vaccine expressing the spike glycoprotein.

    abstract：:The recent identification of a novel human coronavirus responsible of a SARS-like illness in the Middle-East a decade after the SARS pandemic, demonstrates that reemergence of a SARS-like coronavirus from an animal reservoir remains a credible threat. Because SARS is contracted by aerosolized contamination of the resp...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.01.002

    authors： Escriou N,Callendret B,Lorin V,Combredet C,Marianneau P,Février M,Tangy F

    更新日期：2014-03-01 00:00:00

  • A new class of defective hepatitis B virus genomes with an internal poly(dA) sequence.

    abstract：:Sequence heterogeneity of hepatitis B virus (HBV) is increasingly recognized to play a role in virus-host interaction. We have used a recently established method for HBV full-length genome amplification to search for novel types of HBV variants and to investigate further the sequence heterogeneity of HBV genome popula...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8898

    authors： Sommer G,Günther S,Sterneck M,Otto S,Will H

    更新日期：1997-12-22 00:00:00

  • Characterization of the contribution of spliced RNAs of hepatitis B virus to DNA synthesis in transfected cultures of Huh7 and HepG2 cells.

    abstract：:Hepatitis B virus synthesizes multiple spliced RNAs that can be reverse transcribed into viral DNA. We thoroughly characterized the contribution of spliced RNAs to DNA synthesis in transfected cultures of Huh7 and HepG2 cells. We found that up to 50% of DNA within intracellular capsids is derived from five spliced RNA...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.06.021

    authors： Abraham TM,Lewellyn EB,Haines KM,Loeb DD

    更新日期：2008-09-15 00:00:00

  • In vitro strain adaptation of CWD prions by serial protein misfolding cyclic amplification.

    abstract：:We used serial protein misfolding cyclic amplification (sPMCA) to amplify the D10 strain of CWD prions in a linear relationship over two logs of D10 dilutions. The resultant PMCA-amplified D10 induced terminal TSE disease in CWD-susceptible Tg(cerPrP)1536 mice with a survival time approximately 80 days shorter than th...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.09.023

    authors： Meyerett C,Michel B,Pulford B,Spraker TR,Nichols TA,Johnson T,Kurt T,Hoover EA,Telling GC,Zabel MD

    更新日期：2008-12-20 00:00:00

  • Relationship of bovine herpesvirus 1 immediate-early, early, and late gene expression to host cellular gene transcription.

    abstract：:Bovine herpesvirus 1 (BHV-1) gene expression was examined by RNA blot hybridization using clones representing immediate-early, early, and late genes. An immediate-early protein gene probe hybridized with two transcripts, 3.4 and 5.8 kb, expressed by infected cells in the presence of cycloheximide (CH). During infectio...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90744-a

    authors： Seal BS,Whetstone CA,Zamb TJ,Bello LJ,Lawrence WC

    更新日期：1992-05-01 00:00:00

  • Multiple sclerosis retrovirus particles and recombinant envelope trigger an abnormal immune response in vitro, by inducing polyclonal Vbeta16 T-lymphocyte activation.

    abstract：:A retroviral element (MSRV) defining a family of genetically inherited endogenous retroviruses (HERV-W) has recently been characterized in cell cultures from patients with multiple sclerosis (MS). To address the possible relationship with MS, direct detection of circulating virion RNA was proposed but revealed technic...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.1045

    authors： Perron H,Jouvin-Marche E,Michel M,Ounanian-Paraz A,Camelo S,Dumon A,Jolivet-Reynaud C,Marcel F,Souillet Y,Borel E,Gebuhrer L,Santoro L,Marcel S,Seigneurin JM,Marche PN,Lafon M

    更新日期：2001-09-01 00:00:00

  • Partial nucleotide sequence of St. Louis encephalitis virus RNA: structural proteins, NS1, ns2a, and ns2b.

    abstract：:cDNA clones of the St. Louis encephalitis (SLE) virus genome have been obtained and the nucleotide sequence of 4.7 kb corresponding to the 5' terminal half of the genome determined. The genome contains a 5' noncoding region of 98 nucleotides followed by a single continuous open reading frame that encodes three structu...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90409-0

    authors： Trent DW,Kinney RM,Johnson BJ,Vorndam AV,Grant JA,Deubel V,Rice CM,Hahn C

    更新日期：1987-02-01 00:00:00

  • Production and characterization of monoclonal antibodies to porcine group C rotaviruses cross-reactive with group A rotaviruses.

    abstract：:Five monoclonal antibodies (MAbs) to porcine group (gp) C rotaviruses (Cowden and Ah strains) reactive with both gp A and C rotaviruses in cell culture immunofluorescence (CCIF) tests were produced and characterized. These MAbs reacted with three strains of gp A and two strains of gp C rotaviruses in a CCIF test and w...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90189-v

    authors： Tsunemitsu H,Ojeh CK,Jiang B,Simkins RA,Weilnau PA,Saif LJ

    更新日期：1992-11-01 00:00:00

  • Amphibian (Xenopus laevis) tadpoles and adult frogs mount distinct interferon responses to the Frog Virus 3 ranavirus.

    abstract：:Infections of amphibians by Frog Virus 3 (FV3) and other ranavirus genus members are significantly contributing to the amphibian declines, yet much remains unknown regarding amphibian antiviral immunity. Notably, amphibians represent an important step in the evolution of antiviral interferon (IFN) cytokines as they ar...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2017.01.001

    authors： Wendel ES,Yaparla A,Koubourli DV,Grayfer L

    更新日期：2017-03-01 00:00:00

  • Human parainfluenza virus type 2 V protein inhibits interferon production and signaling and is required for replication in non-human primates.

    abstract：:In wild-type human parainfluenza virus type 2 (WT HPIV2), one gene (the P/V gene) encodes both the polymerase-associated phosphoprotein (P) and the accessory V protein. We generated a HPIV2 virus (rHPIV2-V(ko)) in which the P/V gene encodes only the P protein to examine the role of V in replication in vivo and as a po...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.11.018

    authors： Schaap-Nutt A,D'Angelo C,Scull MA,Amaro-Carambot E,Nishio M,Pickles RJ,Collins PL,Murphy BR,Schmidt AC

    更新日期：2010-02-20 00:00:00

  • The in vitro translation of Pea enation mosaic virus RNA.

    abstract：:Pea enation mosaic virus (PEMV) RNA was isolated from virions and then translated in rabbit reticulocyte lysates and in wheat germ extracts. RNA 1 (apparent molecular weight, Mr 1.77 x 106) was shown to code for two major translation products, vp2 (Mr 88,000) and vp4 (Mr 36,000) plus a high-molecular-weight minor prod...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(84)90369-6

    authors： Gabriel CJ,de Zoeten GA

    更新日期：1984-12-01 00:00:00

  • Characterization of a novel alphaherpesvirus associated with fatal infections of domestic rabbits.

    abstract：:A virus was found to be associated with a severe disease affecting rabbits on a farm near Anchorage, Alaska. Extracts from the skin of infected rabbits produced syncytia and cell lysis in cultured rabbit skin, rabbit kidney, and Vero cells. Examination of the infectious agent by electron microscopy revealed an icosahe...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.05.003

    authors： Jin L,Löhr CV,Vanarsdall AL,Baker RJ,Moerdyk-Schauwecker M,Levine C,Gerlach RF,Cohen SA,Alvarado DE,Rohrmann GF

    更新日期：2008-08-15 00:00:00

  • Influenza C and D viral load in cattle correlates with bovine respiratory disease (BRD): Emerging role of orthomyxoviruses in the pathogenesis of BRD.

    abstract：:Bovine respiratory disease (BRD) is the costliest disease affecting the cattle industry globally. Orthomyxoviruses, influenza C virus (ICV) and influenza D virus (IDV) have recently been implicated to play a role in BRD. However, there are contradicting reports about the association of IDV and ICV to BRD. Using the la...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2020.08.014

    authors： Nissly RH,Zaman N,Ibrahim PAS,McDaniel K,Lim L,Kiser JN,Bird I,Chothe SK,Bhushan GL,Vandegrift K,Neibergs HL,Kuchipudi SV

    更新日期：2020-12-01 00:00:00

  • Nucleotide and deduced amino acid sequence of the influenza neuraminidase genes of two equine serotypes.

    abstract：:Equine influenza is caused by two serotypes of type A influenza virus, EIV-A1 and EIV-A2. The complete nucleotide sequence of the neuraminidase (NA) genes of both the A1 (N7 subtype) and A2 (N8 subtype) serotype has been determined following cloning of full-length viral NA cDNAs into pBR322. Analysis of the deduced am...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90207-2

    authors： Dale B,Brown R,Miller J,White RT,Air GM,Cordell B

    更新日期：1986-12-01 00:00:00