Abstract:
:Among 167 rotavirus specimens collected from young children in a suburban area of Bissau, Guinea-Bissau, from 1996 to 1998, most identifiable strains belonged to the uncommon P[6], G2 type and approximately 50% remained incompletely typed. In the present study, 76 such strains were further characterized. Due to interprimer interaction during the standard multiplex PCR approach, modifications of this procedure were implemented. The modified analyses revealed a high frequency of G2, G8, and G9 genotypes, often combined with P[4] and/or P[6]. The Guinean G8 and G9 strains were 97 and 98%, respectively, identical to other African G8 and G9 strains. Multiple G and/or P types were identified at a high frequency (59%), including two previously undescribed mixed infections, P[4]P[6], G2G8 and P[4]P[6], G2G9. These mixed infections most likely represent naturally occurring reassortance of rotavirus strains. Detection of such strains among the previously incompletely typed strains indicates a potential underestimation of mixed infections, if only a standard multiplex PCR procedure is followed. Furthermore cross-priming of the G3 primer with the G8 primer binding site and silent mutations at the P[4] and P[6] primer binding sites were detected. These findings highlight the need for regular evaluation of the multiplex primer PCR method and typing primers. The high frequency of uncommon as well as reassortant rotavirus strains in countries where rotavirus is an important cause of child mortality underscores the need for extensive strain surveillance as a basis to develop appropriate rotavirus vaccine candidates.
journal_name
Virologyjournal_title
Virologyauthors
Fischer TK,Page NA,Griffin DD,Eugen-Olsen J,Pedersen AG,Valentiner-Branth P,Mølbak K,Sommerfelt H,Nielsen NMdoi
10.1016/s0042-6822(03)00153-3subject
Has Abstractpub_date
2003-06-20 00:00:00pages
125-33issue
1eissn
0042-6822issn
1096-0341pii
S0042682203001533journal_volume
311pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::A full-length cDNA clone of the U1 (common) strain of tobacco mosaic virus (TMV) was constructed, and highly infectious transcripts were produced in vitro using bacteriophage T7 RNA polymerase. Frameshift mutations designed to cause premature termination of translation were introduced into either the 30-kDa movement p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90475-q
更新日期:1991-03-01 00:00:00
abstract::Visna is a slow virus infection of sheep in which the characteristic pathological change is demyelination in foci of inflammation. The latter is thought to be the result of an immunopathological process directed against cellular and antigenic targets that have been difficult to define because of restricted viral gene ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90264-8
更新日期:1985-03-01 00:00:00
abstract::Rift Valley fever virus-induced protein synthesis was examined by polyacrylamide gel electrophoresis and fluorography. Five virus-induced polypeptides were detected, the nucleocapsid protein N, the nucleus-associated nonstructural protein NS1, the glycoproteins G1 and G2, and a protein of molecular weight 80K. The N, ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90277-0
更新日期:1984-04-15 00:00:00
abstract::The single-stranded DNA-binding protein LEF-3 of Autographa californica multinucleocapsid nucleopolyhedrovirus consists of 385 amino acid residues, forms oligomers, and promotes Mg2+-independent unwinding of DNA duplexes and annealing of complementary DNA strands. Partial proteolysis revealed that the DNA-binding doma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.027
更新日期:2006-03-15 00:00:00
abstract::Vitis vinifera cultivars Zinfandel-1A and Mission were found to harbor different grapevine yellow speckle viroid-1 (GYSVd-1) variants and characterized to define the relationship to yellow speckle (YS) and vein-banding (VB) diseases. Products from the left terminal (T1), pathogenic (P), and a portion of the central (C...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9292
更新日期:1998-09-01 00:00:00
abstract::Functions of the six Epstein-Barr virus latent infection nuclear proteins (EBNA-1, -2, -3A, -3B, -3C, or -LP) in maintaining latent infection or cell growth transformation are only partially understood. Using antibodies specific for each EBNA in immunofluorescence microscopy, EBNA-2, -3A, and -3C localized to subnucle...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90027-o
更新日期:1990-06-01 00:00:00
abstract::The retroviral integrase (IN) is a virus-encoded enzyme that is essential for insertion of viral DNA into the host chromosome. In order to map and define the properties of a minimal functional domain for this unique viral enzyme, a series of N- and C-terminal deletions of both Rous sarcoma virus (RSV) and human immuno...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80060-3
更新日期:1995-01-10 00:00:00
abstract::To elucidate the mechanism of formation of cowpea mosaic virus (CPMV) particles, RNA-2-encoded precursor proteins were expressed in Spodoptera frugiperda cells. Processing of the 105K and 95K polyproteins in trans to give the mature Large (L) and Small (S) coat proteins required both the 32K proteinase cofactor and th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.08.023
更新日期:2009-10-25 00:00:00
abstract::The binding of influenza virus to erythrocytes and host cells is mediated by the interaction of the viral hemagglutinin (H) with cell surface receptors containing sialic acid (SA). The specificity of this interaction for 19 human and animal influenza isolates was examined using human erythrocytes enzymatically modifie...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90150-2
更新日期:1983-06-01 00:00:00
abstract::The filoviruses Ebolavirus (EBOV) and Marburgvirus (MARV) cause severe hemorrhagic fever in humans and are potential agents of biological warfare. The envelope glycoprotein (GP) of filoviruses mediates viral entry into cells and is an attractive target for therapeutic intervention and vaccine design. Here, we asked if...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.04.038
更新日期:2006-09-01 00:00:00
abstract::To investigate the role of alfalfa mosaic virus coat protein (CP) in genome activation, asymmetric plus-strand RNA accumulation, and cell-to-cell spread of the virus, mutations were made in the CP gene and putative CP binding sites in the 3'-untranslated region (UTR) of RNA 3. Mutants that produced no CP-related pepti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1411
更新日期:1994-08-01 00:00:00
abstract::Mouse hepatitis virus strain A59 (MHV-A59) encodes within the 22-kb gene 1 a large polyprotein containing three proteinase domains with proven or predicted cysteine catalytic residues. E64d, a specific, irreversible inhibitor of cysteine (thiol) proteinases, inhibits the processing of the gene 1 polyprotein. Specifica...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1123
更新日期:1995-04-01 00:00:00
abstract::Although it is known that Ad5-specific neutralizing antibodies (NAbs) against three major viral capsid components (hexon, penton and fiber) are generated, differences in the frequency and nature of these pre-existing NAbs remain unclear. The results emphasized the contribution of anti-fiber antibodies to Ad5 neutraliz...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.12.014
更新日期:2013-03-15 00:00:00
abstract::The 50-70 S RNA complex of a clone of Kirsten murine leukemia virus (Ki-MLV), propagated in NIH 3T3 cells, as well as that of a Moloney (Mo)-MLV, propagated in a mouse thymus-bone marrow cell line, contained an approximately 30 S RNA of unknown biological activity in addition to 38 S genomic RNA. The approximately 30 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(77)90225-2
更新日期:1977-11-01 00:00:00
abstract::The cellular protein Cyclophilin A (Cyp A) is packaged into human immunodeficiency virus type 1 (HIV-1) particles through a specific interaction with the capsid domain of the Gag polyprotein. Inhibition of Cyp A incorporation by mutagenesis or cyclosporin treatment severely affects infectivity of all HIV-1 M subtypes ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1347
更新日期:2002-03-15 00:00:00
abstract::We have cloned an infectious genome of the Junonia coenia densonucleosis virus (JcDNV) into the bacterial plasmid pBR322. The viral genome could be rescued from the recombinant plasmid pBRJ by transfection of pBRJ DNA to sensitive Spodoptera littoralis larvae. pBRJ DNA produced a typical viral infection and a comparab...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90308-e
更新日期:1990-11-01 00:00:00
abstract::The human apolipoprotein B mRNA editing enzyme catalytic peptide-like 3 (APOBEC3; A3) family of proteins (A3A-H) are known to restrict various retroviruses and retroelements, but the full complement of rhesus macaque A3 proteins remains unclear. We report the isolation and characterization of the hA3A homologue from r...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.07.017
更新日期:2011-10-10 00:00:00
abstract::Dengue virus (DENV) is an arbovirus that belongs to the Flaviviridae family. Studies reveal that peptides secreted by amphibians have many functions, such as antiviral and antimicrobial activities. As there is no antiviral drug effective against the DENV, the antiviral activity of a synthetic peptide called HS-1, deri...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.11.009
更新日期:2018-01-15 00:00:00
abstract::The sequence of gene VI from figwort mosaic virus (FMV) clone x4 was determined and compared with that previously published for FMV clone DxS. Both clones originated from the same virus isolation, but the virus used to clone DxS was propagated extensively in a host of a different family prior to cloning whereas that u...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90624-k
更新日期:1991-06-01 00:00:00
abstract::Computational analysis of feline immunodeficiency virus (FIV) RNA sequences indicated that common FIV strains contain a rev response element (RRE) defined by a long unbranched hairpin with 6 stem-loop sub-domains, termed stem-loop A (SLA). To examine the role of the RNA secondary structure of the RRE, mutational analy...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.04.009
更新日期:2010-09-01 00:00:00
abstract::A novel virus isolated from the feather follicles of cockatoos diagnosed as having psittacine beak and feather disease was characterized by electron microscopy, nucleic acid content, and polypeptide composition. Purified virions displayed an icosahedral symmetry, were nonenveloped, and had a mean diameter of 14 to 16 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90513-8
更新日期:1989-07-01 00:00:00
abstract::Porcine epidemic diarrhea virus (PEDV) is a causative agent of porcine epidemic diarrhea (PED). PED, characterized by acute diarrhea, vomiting, dehydration, has caused serious economic losses in pig industry worldwide. Here, we demonstrate that activation of a disintergrin and metalloprotease 17 (ADAM17) induced the d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.02.001
更新日期:2018-04-01 00:00:00
abstract::The bipartite genomic RNAs of red clover necrotic mosaic virus (RCNMV) lack a 5' cap and a 3' poly(A) tail. RNA1 encodes viral replication proteins, and RNA2 encodes a movement protein (MP). These proteins are translated in a cap-independent manner. We previously identified two cis-acting RNA elements that cooperative...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.06.015
更新日期:2017-09-01 00:00:00
abstract::Previous in vitro work demonstrated the incorporation of the major inducible 70k heat shock protein (i.e., 72k HSP) into the biologically active light nucleocapsid (L-NC) variant of canine distemper virus (CDV). Here, in vitro induction of the cellular stress response, characterized by elevated cytoplasmic and intranu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1072
更新日期:1993-02-01 00:00:00
abstract::In order to investigate the genome organization of the porcine epidemic diarrhea virus (PEDV) further, cDNA clones covering the region between the nucleocapsid and the spike (S) protein genes were independently constructed and sequenced for the two virulent isolates Br1/87 and CV777. Of the three major ORFs identified...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1058
更新日期:1994-02-01 00:00:00
abstract::The HIV-1 Nef protein causes a decrease in major histocompatibility complex (MHC) class I and CD4 molecule expression on the cell surface. To determine if Nef can affect components of the innate immune response, we assessed the ability of Nef to alter the cell surface expression of human CD1d. In cells co-expressing C...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.04.020
更新日期:2005-07-05 00:00:00
abstract::The promotion of membrane fusion by the paramyxovirus hemagglutinin-neuraminidase (HN) and fusion (F) proteins requires that they be derived from homologous viruses, suggesting the possibility that the promotion of fusion requires a virus-specific communication between the two glycoprotein spikes. We have evaluated th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1278
更新日期:1995-06-01 00:00:00
abstract::Although herpesviruses have a wide host range and their genomes vary substantially in size, the nucleocapsid appears to be a conservative element of viral design. The capsid shell is icosahedrally symmetric (T = 16), and 125 nm in diameter and 15nm thick in the case of herpes simplex virus 1 (HSV-1). Channel catfish v...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0016
更新日期:1996-01-15 00:00:00
abstract::The protein content of vaccinia virus mature virions, purified by rate zonal and isopycnic centrifugations and solubilized by SDS or a solution of urea and thiourea, was determined by the accurate mass and time tag technology which uses both tandem mass spectrometry and Fourier transform-ion cyclotron resonance mass s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.08.025
更新日期:2007-02-05 00:00:00
abstract::Cucumber mosaic virus (CMV), generated from biologically active cDNA clones of Fny-CMV RNA 1 plus 2 and Sny-CMV RNA 3, derived from the Fny- and Sny-strains of CMV, was able to infect tobacco but not squash plants systemically. In squash, viral RNA, movement protein, and coat protein all accumulated in the inoculated ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8468
更新日期:1997-04-14 00:00:00