Abstract:
:Infection of mice with coxsackievirus B4 results within days in a severe acute necrotizing pancreatitis, which resolves completely within weeks. Gelatinase B or matrix metalloproteinase 9 (MMP-9) has previously been shown to be involved in several models of pancreatitis, but its role in virus-induced pancreatitis has never been investigated. We here report that MMP-9 levels are markedly increased in the pancreas of mice that developed acute pancreatitis following infection with coxsackievirus B4. Moreover, using in situ zymography, we demonstrated that MMP-9 is active in vivo. Double immunohistochemical analysis revealed that macrophages and neutrophils were the cellular source of MMP-9. Extensive tissue rearrangements involving collagen turnover were observed, and these were associated with extensive pathology and resolution of the disease. In summary, this report demonstrates that acute coxsackievirus B4-induced pancreatitis involves the action of MMP-9, which is mainly originating from macrophages and neutrophils.
journal_name
Virologyjournal_title
Virologyauthors
De Palma AM,Verbeken E,Van Aelst I,Van den Steen PE,Opdenakker G,Neyts Jdoi
10.1016/j.virol.2008.08.046subject
Has Abstractpub_date
2008-12-05 00:00:00pages
20-7issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(08)00566-7journal_volume
382pub_type
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