The p.Ser267Phe variant of sodium taurocholate cotransporting polypeptide (NTCP) supports HBV infection with a low efficiency.

abstract：:A combination of linker scanning mutagenesis and deletional analyses has been used to determine the role of individual DNA-protein binding sites on expression from the hepatitis B virus (HBV) enhancer I-X promoter (map position (mp) 1042-1354, HBV adw2). Linker scanning mutation of the EF-C site caused a 67.5% drop in...

journal_title：Virology

authors： Gustin K,Shapiro M,Lee W,Burk RD

更新日期：1993-04-01 00:00:00

abstract：:Posttranslational modification of viral proteins by cellular enzymes is a feature of many virus replication strategies. Here, we report that during infection the multifunctional human influenza A virus NS1 protein is phosphorylated at threonine-215. Substitution of alanine for threonine at this position reduced early ...

journal_title：Virology

authors： Hale BG,Knebel A,Botting CH,Galloway CS,Precious BL,Jackson D,Elliott RM,Randall RE

更新日期：2009-01-05 00:00:00

abstract：:Classical swine fever virus (CSFV) is a positive-sense RNA virus with a high degree of genetic variability among isolates. High diversity is also found in virulence, with strains covering the complete spectrum from avirulent to highly virulent. The underlying genetic determinants are far from being understood. Since R...

journal_title：Virology

authors： Töpfer A,Höper D,Blome S,Beer M,Beerenwinkel N,Ruggli N,Leifer I

更新日期：2013-03-30 00:00:00

abstract：:The genome of the single-stranded DNA phage f1 contains an intergenic region (IG), 508-nucleotides long, that does not code for any known protein. By use of a system of chimeric plasmids haboring different f1 fragments, we had previously shown that this region contains, in addition to the f1 'functional origin' of DNA...

journal_title：Virology

authors： Dotto GP,Zinder ND

更新日期：1983-10-15 00:00:00

abstract：:Although rodents are important reservoirs for RNA viruses, to date only one species of rodent coronavirus (CoV) has been identified. Herein, we describe a new CoV, denoted Lucheng Rn rat coronavirus (LRNV), and novel variants of two Betacoronavirus species termed Longquan Aa mouse coronavirus (LAMV) and Longquan Rl ra...

journal_title：Virology

authors： Wang W,Lin XD,Guo WP,Zhou RH,Wang MR,Wang CQ,Ge S,Mei SH,Li MH,Shi M,Holmes EC,Zhang YZ

更新日期：2015-01-01 00:00:00

abstract：:RNA interference (RNAi) is the process by which double-stranded RNA (dsRNA) directs sequence-specific degradation of messenger RNA in animal and plant cells. In mammalian cells, RNAi can be triggered by 21-23 nucleotide duplexes of small interfering RNA (siRNA). Strategies to inhibit RNA virus multiplication based on ...

journal_title：Virology

authors： Liu M,Chen W,Ni Z,Yan W,Fei L,Jiao Y,Zhang J,Du Q,Wei X,Chen J,Liu Y,Zheng Z

更新日期：2005-05-25 00:00:00

abstract：:Many antiviral drugs must be metabolized to their active form by cellular enzymes. Their antiviral activity may therefore be limited by an inefficient metabolism, leading to low intracellular concentration of the active form or to the accumulation of toxic intermediate metabolites. Gene transfer might be used to overc...

journal_title：Virology

authors： Guettari N,Loubière L,Brisson E,Klatzmann D

更新日期：1997-09-01 00:00:00

abstract：:We have determined the nucleotide sequence of the RNA2 segment of the Nudaurelia capensis omega virus genome. It was found to consist of 2448 nucleotides and contained one long open reading frame (ORF) encoding the 644 residue capsid protein. The deduced amino acid sequence of this protein reveals a positively charged...

journal_title：Virology

authors： Agrawal DK,Johnson JE

更新日期：1992-10-01 00:00:00

abstract：:Although lentiviruses like HIV-1 are able to infect non-dividing cells, particular resting cells such as non-stimulated primary peripheral blood mononuclear cells (PBMC) are resistant to infection. In contrast to other lentiviruses, SIVsmmPBj can replicate in non-stimulated PBMC. Moreover, SIVsmmPBj-derived, but not H...

journal_title：Virology

authors： Wolfrum N,Mühlebach MD,Schüle S,Kaiser JK,Kloke BP,Cichutek K,Schweizer M

更新日期：2007-08-01 00:00:00

abstract：:Tobacco mosaic virus (TMV) RNA was encapsulated in large unilamellar vesicles (LUV) of phosphatidylserine and was introduced into protoplasts from Vinca rosea suspension cultures. Infection could be effected by brief incubation of protoplasts with the LUV containing TMV-RNA in the presence of either polyethylene glyco...

journal_title：Virology

authors： Fukunaga Y,Nagata T,Takebe I

更新日期：1981-09-01 00:00:00

abstract：:In previous studies, a mutant (cp-RSV) of the RSV A2 strain derived from 52 serial cold passages in bovine embryonic tissue culture was highly attenuated in seropositive adults and children but caused upper respiratory tract disease in seronegative infants. We investigated the genetic basis for this attenuation phenot...

journal_title：Virology

authors： Connors M,Crowe JE Jr,Firestone CY,Murphy BR,Collins PL

更新日期：1995-04-20 00:00:00

abstract：:p53 is implicated in several cellular pathways such as induction of cell-cycle arrest, differentiation, senescence, and apoptosis. p53 is activated by a broad range of stress signals, including viral infections. While some viruses activate p53, others induce its inactivation, and occasionally p53 is differentially mod...

journal_title：Virology

authors： Trujillo-Uscanga A,Gutiérrez-Escolano AL

更新日期：2020-11-01 00:00:00

abstract：:To begin to map functional domains of the Sendai P-L RNA polymerase complex we wanted to characterize the P binding site on the Sendai L protein. Analysis of in vitro and in vivo P-L polymerase complex formation with carboxyl-truncations of the L protein showed that the N-terminal half of the protein was required. Sit...

journal_title：Virology

authors： Chandrika R,Horikami SM,Smallwood S,Moyer SA

更新日期：1995-11-10 00:00:00

abstract：:Activation of the minute virus of mice (MVM) P4 promoter is a key step in the life cycle of the virus and is completely dependent on host transcription factors. Since transcription-factor composition varies widely in different cell types, there is the possibility that only some cell types in the host organism have the...

journal_title：Virology

authors： Davis C,Segev-Amzaleg N,Rotem I,Mincberg M,Amir N,Sivan S,Gitelman I,Tal J

更新日期：2003-02-15 00:00:00

abstract：:We have constructed a recombinant baculovirus expressing high levels of the herpes simplex virus type 1 (HSV-1) glycoprotein E (gE) in Sf9 cells. The expressed gE migrated on gels as a double band with apparent molecular weights of 68 and 70 kDa. The recombinant gE was glycosylated based on its susceptibility to tunic...

journal_title：Virology

authors： Ghiasi H,Kaiwar R,Nesburn AB,Slanina S,Wechsler SL

更新日期：1992-06-01 00:00:00

abstract：:This report shows that (S)CARNA 5, the satellite of cucumber mosaic virus (CMV) strain S, directs the synthesis of two small proteins in a wheat germ cell-free translation system. The two proteins are produced by the translation of both (S)CARNA 5 purified from CMV virions and the (+) strand purified from double-stran...

journal_title：Virology

authors： Avila-Rincon MJ,Collmer CW,Kaper JM

更新日期：1986-07-30 00:00:00

abstract：:The function of the 16-kDa protein encoded by tobacco rattle virus (TRV) RNA-1 was investigated by a mutational analysis of the 16-kDa protein gene. Transcripts of TRV RNA-1 produced from a full-length cDNA clone of TRV RNA-1 (SYM strain) remained infectious when the 16-kDa protein gene was disrupted by premature term...

journal_title：Virology

authors： Guilford PJ,Ziegler-Graff V,Baulcombe DC

更新日期：1991-06-01 00:00:00

abstract：:The cowpea strain of Southern bean mosaic virus (SBMV-C), a T = 3 icosahedral RNA virus, was dissociated to yield a ribonucleoprotein complex (RNPC) composed of the viral RNA and coat protein subunits. To determine if the coat protein subunits were bound to a specific site on the viral RNA, the RNPC was treated with r...

journal_title：Virology

authors： Hacker DL

更新日期：1995-03-10 00:00:00

abstract：:The measles virus RNA-dependent RNA polymerase consists of two virus-encoded subunits, the phosphoprotein (P) and the large (L) protein. The P mRNA also codes for a C protein in the +1 reading frame relative to P. The activities of the measles P and C proteins from the vaccine strain, EdB, a wild-type CM strain, and a...

journal_title：Virology

authors： Reutter GL,Cortese-Grogan C,Wilson J,Moyer SA

更新日期：2001-06-20 00:00:00

abstract：:The glycoprotein E (gE) of varicella zoster virus (VZV), encoded by ORF68, is the most abundant viral glycoprotein. In the current experiments, we demonstrated that ORF68 deletion was incompatible with recovery of infectious virus from VZV cosmids. Replacing ORF68 at a nonnative AvrII site in the genome restored infec...

journal_title：Virology

authors： Mo C,Lee J,Sommer M,Grose C,Arvin AM

更新日期：2002-12-20 00:00:00

abstract：:The molecular analysis of three ovine pestivirus strains revealed the existence of two distinct groups of sheep-derived pestiviruses, namely "true" border disease virus strains (BDV) and bovine viral diarrhea virus (BVDV)-like strains. As an extension of these studies RT-PCR and nucleotide sequencing of the autoprotea...

journal_title：Virology

authors： Becher P,König M,Paton DJ,Thiel HJ

更新日期：1995-05-10 00:00:00

abstract：:Mice inoculated with replication-competent stocks of the murine acquired immunodeficiency syndrome (MAIDS) virus are severely immunocompromised and proned to the development of T- and B-cell lymphomas. We have studied the development of T-cell lymphomas in C57BL/6 and RF/J mice inoculated with helper-free stocks of th...

journal_title：Virology

authors： Simard C,Huang M,Jolicoeur P

更新日期：1995-01-10 00:00:00

abstract：:Old world hantaviruses, causing hemorrhagic fever with renal syndrome (HFRS), still present a public health problem in Asia and Eastern Europe. The majority of cases has been recorded in China. The aim of our study was to generate human recombinant neutralizing antibodies to a hantavirus by phage display technology. T...

journal_title：Virology

authors： Koch J,Liang M,Queitsch I,Kraus AA,Bautz EK

更新日期：2003-03-30 00:00:00

abstract：:Condylomata acuminata is caused by human papillomavirus (HPV). PCR with consensus primers will typically detect HPV in >96% of condylomata. Metagenomic sequencing has found that some "HPV-negative" condylomata do indeed contain HPV. We wished to perform a renewed evaluation of the "HPV-negative" condylomata using deep...

journal_title：Virology

authors： Arroyo Mühr LS,Bzhalava D,Lagheden C,Eklund C,Johansson H,Forslund O,Dillner J,Hultin E

更新日期：2015-11-01 00:00:00

abstract：:A cDNA was constructed to encode an internally-truncated version of negative-sense genomic RNA (vRNA) of respiratory syncytial virus (RSV) containing (in 3' to 5' order) the 3' vRNA leader region, the complement of the open reading frame for bacterial chloramphenicol acetyl transferase (CAT) under the control of RSV g...

journal_title：Virology

authors： Collins PL,Mink MA,Hill MG 3rd,Camargo E,Grosfeld H,Stec DS

更新日期：1993-07-01 00:00:00

abstract：:Particles purified from the liver of hepatitis B virus-infected patients were previously shown by us to incorporate 32P-deoxynucleotides into viral DNA and DNA-RNA hybrid molecules when incubated in a DNA polymerase reaction mixture. In this investigation, similar particles from duck and ground squirrel livers infecte...

journal_title：Virology

authors： Miller RH,Marion PL,Robinson WS

更新日期：1984-11-01 00:00:00

abstract：:The DNA of a rat cytomegalovirus (RCMV) isolate from England is cleaved by HindIII into 25 fragments, 20 of which have been cloned into recombinant plasmids. Twenty-two of thirty KpnI fragments were similarly cloned which, together with the HindIII clones, resulted in cloning of 93% of the viral genome. Terminal fragm...

journal_title：Virology

authors： Burns WH,Barbour GM,Sandford GR

更新日期：1988-09-01 00:00:00

abstract：:In this article we report the initial biochemical, genetic, and electron microscopic analysis of a previously uncharacterized, 8.9-kDa, predicted thiol-redox protein. The name A2.5L was assigned to the corresponding vaccinia virus gene, which is conserved in all sequenced poxviruses. Multiple alignment analysis and se...

journal_title：Virology

authors： Senkevich TG,White CL,Weisberg A,Granek JA,Wolffe EJ,Koonin EV,Moss B

更新日期：2002-09-01 00:00:00

abstract：:Viruses exploit phosphorylation of both viral and host proteins to support viral replication. In this study, we demonstrate that porcine reproductive and respiratory syndrome virus replicase nsp2, and two nsp2-related -2/-1 frameshifting products, nsp2TF and nsp2N, are hyper-phosphorylated. By mapping phosphorylation ...

journal_title：Virology

authors： Shang P,Yuan F,Misra S,Li Y,Fang Y

更新日期：2020-04-01 00:00:00

abstract：:The longest open reading frame of turnip yellow mosaic virus genomic RNA (ORF-206) encodes a 206-kDa nonstructural protein. The most prominent in vitro translation products of ORF-206 are the full-length p206 and a shorter N-coterminal 150-kDa protein. We have confirmed these assignments by immunoprecipitation of in v...

journal_title：Virology

authors： Bransom KL,Weiland JJ,Dreher TW

更新日期：1991-09-01 00:00:00