Abstract:
:Although lentiviruses like HIV-1 are able to infect non-dividing cells, particular resting cells such as non-stimulated primary peripheral blood mononuclear cells (PBMC) are resistant to infection. In contrast to other lentiviruses, SIVsmmPBj can replicate in non-stimulated PBMC. Moreover, SIVsmmPBj-derived, but not HIV-1-derived, replication-incompetent vectors enable gene transfer into G(0)-arrested human cell lines and primary human monocytes. Here, we demonstrate that transduction of G(0)-arrested cell lines by SIVsmmPBj-derived vectors is independent of the viral accessory proteins Vif, Vpx, Vpr, or Nef. In contrast, for the transduction of primary human monocytes, the Vpx protein proved to be essential. However, trans-complementation of HIV-1 vectors with SIVsmmPBj Vpx did not provide the property of gene transfer into monocytes. Taken together, these data indicate that Vpx is essential for the infection of primary monocytes by SIVsmmPBj. Additionally, further genome functions besides the accessory proteins are required for the particular capacity of SIVsmmPBj in transduction or infection events.
journal_name
Virologyjournal_title
Virologyauthors
Wolfrum N,Mühlebach MD,Schüle S,Kaiser JK,Kloke BP,Cichutek K,Schweizer Mdoi
10.1016/j.virol.2007.03.008subject
Has Abstractpub_date
2007-08-01 00:00:00pages
330-41issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(07)00152-3journal_volume
364pub_type
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