Construction and analysis of infectious transcripts synthesized from full-length cDNA clones of both genomic RNAs of pea early browning virus.

abstract：:Pre-exposure prophylaxis (PrEP) strategies utilizing anti-retroviral drugs show considerable promise for HIV prevention. However there is insufficient pharmacokinetic (PK) data on drug concentrations required for protection at the relevant mucosal tissues where the infection is initiated. Here we evaluated the utility...

journal_title：Virology

authors： Veselinovic M,Yang KH,LeCureux J,Sykes C,Remling-Mulder L,Kashuba ADM,Akkina R

更新日期：2014-09-01 00:00:00

abstract：:Syncytia formation has been considered important for cell-to-cell spread and pathogenesis of many viruses. As a syncytium forms, individual nuclei often congregate together, allowing close contact of nuclear membranes and possibly fusion to occur. However, there is currently no reported evidence of nuclear membrane fu...

journal_title：Virology

authors： Wang W,Yang L,Huang X,Fu W,Pan D,Cai L,Ye J,Liu J,Xia N,Cheng T,Zhu H

更新日期：2017-12-01 00:00:00

abstract：:Infection of microglial cells by the human immunodeficiency virus (HIV) is supposed to play an important role in the pathogenesis of AIDS-related central nervous system (CNS) complications. So far, however, experimental data about interactions between HIV and ramified microglia from the adult CNS were only occasionall...

journal_title：Virology

authors： Hein A,Martin JP,Koehren F,Bingen A,Dörries R

更新日期：2000-03-15 00:00:00

abstract：:Two sets of primers derived from the 5'-terminal region and the NS5 region of the hepatitis G virus (HGV) genome were used to amplify PCR fragments from serum specimens obtained from different parts of the world. All PCR fragments from the 5'-terminal region (5'-PCR, n = 56) and from the NS5 region (NS5-PCR, n = 85) w...

journal_title：Virology

authors： Cong M,Fried MW,Lambert S,Lopareva EN,Zhan M,Pujol FH,Thyagarajan SP,Byun KS,Fields HA,Khudyakov YE

更新日期：1999-03-15 00:00:00

abstract：:HIV CRF07 B'/C is a strain circulating mainly in northwest region of China. The gp41 region of CRF07 is derived from a clade C virus. In order to compare the difference of CRF07 gp41 with that of typical clade B virus, we solved the crystal structure of the core region of CRF07 gp41. Compared with clade B gp41, CRF07 ...

journal_title：Virology

authors： Du J,Xue H,Ma J,Liu F,Zhou J,Shao Y,Qiao W,Liu X

更新日期：2013-11-01 00:00:00

abstract：:We measured the effects of non-nucleoside reverse transcriptase (RT) inhibitor-resistant mutations K101E+G190S, on replication fitness and EFV-resistance of HIV(NL4-3). K101E+G190S reduced fitness in the absence of EFV and increased EFV resistance, compared to either single mutant. Unexpectedly, K101E+G190S also repli...

journal_title：Virology

authors： Wang J,Liang H,Bacheler L,Wu H,Deriziotis K,Demeter LM,Dykes C

更新日期：2010-07-05 00:00:00

abstract：:The Tat regulatory protein encoded by human immunodeficiency virus type 1 (HIV-1) induces high levels of transcription from the viral long terminal repeat (LTR) promoter element after interacting with a promoter proximal RNA target sequence. In the wild-type HIV-1 LTR, this activation is facilitated by the synergistic...

journal_title：Virology

authors： Madore SJ,Cullen BR

更新日期：1995-02-01 00:00:00

abstract：:The display of foreign proteins on the surface of baculovirus virions has provided a tool for the analysis of protein-protein interactions and for cell-specific targeting in gene transfer applications. To evaluate the baculovirus display system as a vaccine vehicle, we have generated a recombinant baculovirus (AcNPV-C...

journal_title：Virology

authors： Yoshida S,Kondoh D,Arai E,Matsuoka H,Seki C,Tanaka T,Okada M,Ishii A

更新日期：2003-11-10 00:00:00

abstract：:The complete RNA sequence of the hepatitis delta virus (HDV) obtained from the Nauru Island in the Pacific was determined by cDNA cloning and amplification by polymerase chain reaction (PCR). The sequence showed 14-17% divergence from the two known HDV RNA sequences. There are three highly conserved domains: the regio...

journal_title：Virology

authors： Chao YC,Chang MF,Gust I,Lai MM

更新日期：1990-10-01 00:00:00

abstract：:The extraordinarily high level of genetic variation of HIV-1 env genes poses a challenge to obtain antibodies that cross-react with multiple subtype Env glycoproteins. To determine if cross-reactive monoclonal antibodies (mAbs) to highly conserved epitopes in HIV-1 envelope glycoproteins can be induced, we immunized m...

journal_title：Virology

authors： Gao F,Scearce RM,Alam SM,Hora B,Xia S,Hohm JE,Parks RJ,Ogburn DF,Tomaras GD,Park E,Lomas WE,Maino VC,Fiscus SA,Cohen MS,Moody MA,Hahn BH,Korber BT,Liao HX,Haynes BF

更新日期：2009-11-10 00:00:00

abstract：:The equine herpesvirus 1 (EHV-1) immediate-early (IE) phosphoprotein is essential for the activation of transcription from viral early and late promoters and regulates transcription from its own promoter. The IE protein of 1487 amino acids contains a serine-rich tract (SRT) between residues 181 and 220. Deletion of th...

journal_title：Virology

authors： Kim SK,Buczynski KA,Caughman GB,O'Callaghan DJ

更新日期：2001-01-05 00:00:00

abstract：:Kaposi's sarcoma (KS)-associated herpesvirus (KSHV) vFLIP, a latent gene of KSHV, was first identified as a FLICE-inhibitory protein (FLIP) protecting cells from apoptosis. The vFLIP protein has been shown to activate the NF-κB signaling involved in spindle morphology formation both in HUVECs infected with KSHV and Ka...

journal_title：Virology

authors： Yang Z,Honda T,Ueda K

更新日期：2018-09-01 00:00:00

abstract：:Hypervariable region 1 (HVR1) proteins of hepatitis C virus (HCV) have been reported to react broadly with sera of patients with HCV infection. However, the variability of the broad reactivity of individual HVR1 proteins has not been elucidated. We assessed the reactivity of 25 different HVR1 proteins (genotype 1b) wi...

journal_title：Virology

authors： Watanabe K,Yoshioka K,Ito H,Ishigami M,Takagi K,Utsunomiya S,Kobayashi M,Kishimoto H,Yano M,Kakumu S

更新日期：1999-11-10 00:00:00

abstract：:Immunization with heat-inactivated herpes simplex virus type 1 (HSV-1) 2-5 days before ocular infection reduced the frequency of establishment of latent HSV-1 infection in the trigeminal ganglion (TG); this induction of resistance coincided with reduced expression of IFN-gamma mRNA in the TG. Immunization with unrelat...

journal_title：Virology

authors： Halford WP,Veress LA,Gebhardt BM,Carr DJ

更新日期：1997-09-29 00:00:00

abstract：:Previous studies with monoclonal antibodies of the antigenic structure of bovine leukemia virus (BLV) envelope glycoprotein (gp51) have identified three epitopes (F, G, H) directly involved in the infectivity of BLV, F, G, and H lost their reactivity with the respective monoclonal antibodies after treatment with a red...

journal_title：Virology

authors： Portetelle D,Couez D,Bruck C,Kettmann R,Mammerickx M,Van der Maaten M,Brasseur R,Burny A

更新日期：1989-03-01 00:00:00

abstract：:The amino acid sequence of the coat protein of wild-type cowpea chlorotic mottle virus has been nearly completely determined by direct methods. The sites of amino acid replacements were identified in the coat proteins of an oxidation-sensitive mutant, a temperature-sensitive mutant, and a salt-stable mutant. The repla...

journal_title：Virology

authors： Rees MW,Short MN

更新日期：1982-06-01 00:00:00

abstract：:It is well established that the human subgroup C adenovirus type 5 (Ad5) E1B 55 kDa protein can regulate the activity and concentration of the cellular tumor suppressor, p53. However, the contribution(s) of these functions of the E1B protein to viral reproduction remains unclear. To investigate this issue, we examined...

journal_title：Virology

authors： Cardoso FM,Kato SE,Huang W,Flint SJ,Gonzalez RA

更新日期：2008-09-01 00:00:00

abstract：:In a previous paper (G. Winkler, V. B. Randolph, G. R. Cleaves, T. E. Ryan, and V. Stollar, 1988, Virology 162, 187-196) we showed that the newly synthesized dengue-2 virus nonstructural protein, NS1, exists briefly as a monomer and then undergoes dimerization. We demonstrate here that the dimerization of NS1 is assoc...

journal_title：Virology

authors： Winkler G,Maxwell SE,Ruemmler C,Stollar V

更新日期：1989-07-01 00:00:00

abstract：:DNA immunization in conjunction with antiretroviral therapy was evaluated in SIV-infected rhesus macaques treated with [R]-9-[2-phosphonylmethoxypropyl]adenine (PMPA). Macaques were immunized monthly with DNA vaccines expressing either SIV gag/tat or SIV gag/tat and 19 CD8+ T cell epitopes during 7 months of therapy. ...

journal_title：Virology

authors： Fuller DH,Rajakumar PA,Wu MS,McMahon CW,Shipley T,Fuller JT,Bazmi A,Trichel AM,Allen TM,Mothe B,Haynes JR,Watkins DI,Murphey-Corb M

更新日期：2006-04-25 00:00:00

abstract：:Nucleotide sequence analysis has shown previously that the genomic-length mRNA (mRNA1) of the coronavirus infectious bronchitis virus (IBV) contains two large open reading frames (ORFs), 1a and 1b, with the potential to encode polyproteins of approximately 441 and 300 kDa, respectively. We have characterized the speci...

journal_title：Virology

authors： Liu DX,Tibbles KW,Cavanagh D,Brown TD,Brierley I

更新日期：1995-04-01 00:00:00

abstract：:Polyomaviral vectors are generated by transfecting 293T cells with three sets of DNAs: DNA for the expression of simian virus 40 (SV40) T antigen; DNA for the expression of SV40 capsid proteins, and vector DNA harboring a reporter gene expression cassette carrying a SV40 origin. The vector DNA harbors a minimal sequen...

journal_title：Virology

authors： Nakanishi A,Chapellier B,Maekawa N,Hiramoto M,Kuge T,Takahashi RU,Handa H,Imai T

更新日期：2008-09-15 00:00:00

abstract：:The 121,752-bp genome sequence of bacteriophage T5 was determined; the linear, double-stranded DNA is nicked in one of the strands and has large direct terminal repeats of 10,139 bp (8.3%) at both ends. The genome structure is consistently arranged according to its lytic life cycle. Of the 168 potential open reading f...

journal_title：Virology

authors： Wang J,Jiang Y,Vincent M,Sun Y,Yu H,Wang J,Bao Q,Kong H,Hu S

更新日期：2005-02-05 00:00:00

abstract：:Recombinant baculoviruses containing the coding sequences of the viral structural proteins, i.e., the capsid (C) protein, the precursor to premembrane (preM) protein, and the envelope (E) protein, as well as a nonstructural protein, NS1, of Japanese encephalitis virus (JEV) were constructed. Infection of Spodoptera fr...

journal_title：Virology

authors： Matsuura Y,Miyamoto M,Sato T,Morita C,Yasui K

更新日期：1989-12-01 00:00:00

abstract：:The cellular protein Cyclophilin A (Cyp A) is packaged into human immunodeficiency virus type 1 (HIV-1) particles through a specific interaction with the capsid domain of the Gag polyprotein. Inhibition of Cyp A incorporation by mutagenesis or cyclosporin treatment severely affects infectivity of all HIV-1 M subtypes ...

journal_title：Virology

authors： Wiegers K,Kräusslich HG

更新日期：2002-03-15 00:00:00

abstract：:Long-term non-progressive HIV infection, characterized by low but detectable viral load and stable CD4 counts in the absence of antiviral therapy, is observed in about 5% of HIV-infected patients. Here we identified four therapy naïve individuals who are strongly seropositive for HIV-1 but who lack evidence of detecta...

journal_title：Virology

authors： Kloosterboer N,Groeneveld PH,Jansen CA,van der Vorst TJ,Koning F,Winkel CN,Duits AJ,Miedema F,van Baarle D,van Rij RP,Brinkman K,Schuitemaker H

更新日期：2005-08-15 00:00:00

abstract：:We show that the product of the polarity suppression (psu) gene from bacteriophage P4 associates with P4 capsids. This association can occur when Psu is (i) provided in vivo from the P4 genome or from a plasmid or (ii) provided in vitro by mixing viable phage particles with Psu protein. Psu is unable to associate with...

journal_title：Virology

authors： Isaksen ML,Rishovd ST,Calendar R,Lindqvist BH

更新日期：1992-06-01 00:00:00

abstract：:The complete genomic RNAs of two Korean sacbrood virus (SBV) strains, which infect the honey bee, Apis mellifera, were sequenced. The two sequences (AmSBV-Kor19, AmSBV-Kor21) were distinguished by the presence or absence of a PstI restriction site. These strains comprised of 8784 bp and 8835 bp; contained a single lar...

journal_title：Virology

authors： Choe SE,Nguyen LT,Noh JH,Kweon CH,Reddy KE,Koh HB,Chang KY,Kang SW

更新日期：2012-10-10 00:00:00

abstract：:Thymidine kinase-deficient syrian hamster cells were cotransfected with recombinant plasmids containing the thymidine kinase (TK) gene of Herpes Simplex Virus Type 1, and either intact or partially deleted SV40 T antigen-coding genes. The transformants were selected by their ability to grow in gHAT medium. After selec...

journal_title：Virology

authors： Hirschhorn RR,Mercer WE,Liu HT,Baserga R

更新日期：1984-04-15 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) exploits multiple host factors during its replication. The REV/RRE-dependent nuclear export of unspliced/partially spliced viral transcripts needs the assistance of host proteins. Recent studies have shown that MOV10 overexpression inhibited HIV-1 replication at various step...

journal_title：Virology

authors： Huang F,Zhang J,Zhang Y,Geng G,Liang J,Li Y,Chen J,Liu C,Zhang H

更新日期：2015-12-01 00:00:00

abstract：:While HPV 16 variant lineages have been well characterized, the knowledge about HPV 18 variants is limited. In this study, HPV 18 nucleotide variations in the E2 hinge region were characterized by sequence analysis in 47 control and 51 tumor specimens. Fifty of these specimens were randomly selected for sequencing of ...

journal_title：Virology

authors： Arias-Pulido H,Peyton CL,Torrez-Martínez N,Anderson DN,Wheeler CM

更新日期：2005-07-20 00:00:00