Abstract:
:The molecular mechanism(s) by which human immunodeficiency virus (HIV-1) injures a T-cell line was studied. A pathological role for viral env proteins, which are inserted into the plasma membrane, has been previously demonstrated for HIV as well as other retroviruses which are cytopathic. We therefore initiated studies examining whether perturbations of the cell membrane or membrane-associated biochemical events may be occurring in cells acutely infected with HIV and whether such perturbations, if present, may be responsible for cytopathology. A human T-cell line (ERIC), which is sensitive to the cytopathic effects of HIVs, was infected with HTLV-IIIB and its membrane permeability to cations and its lipid metabolism were studied coincident with the peak expression of viral p24 and with the first sign of cytopathology (slowing of cell division) 72 to 96 hr after infection. It was found that the rate of influx of Ca2+ into the cell increased over that of uninfected cells and that phospholipid synthesis, primarily phosphatidylcholine, became depressed. Diacylglycerol, which serves both as an intermediate for synthesis of phospholipids and as a second-messenger for lymphocyte activation, was also greatly reduced. However, triglyceride synthesis was enhanced, indicating that not all lipid metabolic pathways were being shut down. This decreased membrane-synthetic ability and reduced second-messenger for cell division are likely to be important causes of HIV-1 cytopathology in ERIC cells. This hypothesis was supported by our finding that HIV cytopathology of ERIC cells could be partially prevented by treatment with compounds (diacylglyceride or PMA and transiently by oleic acid) which either replenish diacylglycerol in the infected cell and/or activate protein kinase C or phosphocholine cytidyltransferase, the latter being the rate-limiting step in synthesis of the major structural phospholipid in most cells.
journal_name
Virologyjournal_title
Virologyauthors
Lynn WS,Tweedale A,Cloyd MWdoi
10.1016/0042-6822(88)90232-2subject
Has Abstractpub_date
1988-03-01 00:00:00pages
43-51issue
1eissn
0042-6822issn
1096-0341journal_volume
163pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The RNA dependent RNA polymerase of Sendai virus consists of a complex of the large (L) and phosphoprotein (P) subunits where L is thought to be responsible for all the catalytic activities necessary for viral RNA synthesis. We previously showed that the L protein forms an oligomer [Smallwood, S., Cevik, B., Moyer, S....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.01.032
更新日期:2007-06-20 00:00:00
abstract::The shuttle vector plasmid pZ189 was used as a target for mutagenesis in COS-1 cells. Complex mutations were analyzed in 5 plasmids that were recovered from noninfected cells and 15 plasmids that were recovered from cells that were infected with herpes simplex virus type-1 (HSV-1). Complex mutations in noninfected cel...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90895-i
更新日期:1991-04-01 00:00:00
abstract::Two novel halophilic archaebacterial bacteriophages, HF1 and HF2, were isolated from an Australian solar saltern. They were morphologically identical with icosahedral-shaped heads (diameter 58 nm) and contractile tails (length 94 nm). Other similarities included sensitivity to reduced ionic conditions, similar protein...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1643
更新日期:1993-12-01 00:00:00
abstract::Gene transfer into human hematopoietic stem cells using Ad5 is inefficient due to lack of the primary receptor CAR and the secondary receptors alphavbeta3 integrin and alphavbeta5 integrin, and due to the high seroprevalence of Ad5 antibodies in most adults, resulting in diminished gene transduction. In the present st...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.07.018
更新日期:2004-10-25 00:00:00
abstract::The nucleotide sequence was determined of a 1.6-kb restriction fragment containing the polyhedrin gene of the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata (OpMNPV). In addition, both the 5' and 3' termini of the polyhedrin mRNA were located in the flanking sequences using S1 mapping procedures. 5' Le...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90031-0
更新日期:1986-09-01 00:00:00
abstract::During capsid assembly of bacteriophage P2 and its satellite phage P4, gpN undergoes proteolytic cleavage with the removal of the first 31 amino acids. The truncated protein gpN* is unable to support formation of viable phages in complementation tests. A c-myc antigenic epitope (EQKLISEEDL) exchanged for eleven amino ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0566
更新日期:1996-10-15 00:00:00
abstract::Viral metagenomics coupled to high-throughput sequencing has provided a powerful tool for large-scale detection of known and unknown viruses associated to distinct hosts and environments. Using this approach, known and novel viruses have been characterized from sylvatic and commercial avian hosts, increasing our under...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.05.013
更新日期:2020-09-01 00:00:00
abstract::The 5' leader region of the HIV-1 RNA contains the major 5' splice site (ss) that is used in the production of all spliced viral RNAs. This splice-donor (SD) region can fold a stem-loop structure. We demonstrate that whereas stabilization of this SD hairpin reduces splicing efficiency, destabilization increases splici...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.09.018
更新日期:2014-11-01 00:00:00
abstract::Human papillomavirus type 16 (HPV-16) E2 protein negatively regulates transcription of the E6 and E7 genes. This study was done to test the hypothesis that methylation of the HPV 16 long control region (LCR) is overrepresented among cervical cancer (CaCx) cases compared to cytologically normal controls harboring intac...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.06.018
更新日期:2006-10-25 00:00:00
abstract::Genetic variation in dengue 2 isolates from various geographic areas was examined by oligonucleotide fingerprinting of the 40 S genome RNA. Oligonucleotide maps of geographically isolated and epidemiologically unrelated viruses were very distinct. Direct comparison of the oligonucleotide map of the dengue 2 prototype ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90255-6
更新日期:1983-07-30 00:00:00
abstract::Previously a new small subclass of SV40 large T antigen with a high-binding affinity to living target cells was characterized (J. Lange-Mutschler and R. Henning, 1983, Virology 127, 333-344.) In the present study the external binding process, particularly the tight linkage of T antigen to lipid of the target cells, wa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90176-4
更新日期:1984-07-30 00:00:00
abstract::Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90199-2
更新日期:1989-10-01 00:00:00
abstract::Intact clones containing partial repeats of the genomic components of African cassava mosaic (ACMV DNAs A and B) are infectious when mechanically coinoculated onto Nicotiana benthamiana. Monomeric genomic components may be generated either by homologous recombination or, when two copies of the origin of replication (o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80093-x
更新日期:1995-01-10 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) transmembrane glycoprotein gp41 is targeted by broadly-reactive neutralizing antibodies 2F5 and 4E10, making it an attractive target for vaccine development. To better assess immunogenic properties of gp41, we generated five soluble glutathione S-transferase fusion proteins ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.11.009
更新日期:2008-03-15 00:00:00
abstract::The Human Papillomavirus type-16 (HPV-16) E6 and E7 oncogenes are selectively retained and expressed in cervical carcinomas, and expression of E6 and E7 is sufficient to immortalize human cervical epithelial cells. Expression of the epidermal growth factor receptor (EGFR) is often increased in cervical dysplasia and c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.09.014
更新日期:2011-12-05 00:00:00
abstract::Cell lines persistently infected with foot-and-mouth disease virus (FMDV) have been established by growth of BHK-21 (c-13) or IBRS-2 (c-26) that survived standard cytolytic infections with FMDV. They maintain cytoplasmic FMDV RNA sequences, as shown by dot blot hybridization tests, using cloned FMDV cDNA as probes. Ce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90198-9
更新日期:1985-08-01 00:00:00
abstract::In this article we report the initial biochemical, genetic, and electron microscopic analysis of a previously uncharacterized, 8.9-kDa, predicted thiol-redox protein. The name A2.5L was assigned to the corresponding vaccinia virus gene, which is conserved in all sequenced poxviruses. Multiple alignment analysis and se...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1608
更新日期:2002-09-01 00:00:00
abstract::In this paper we present a technique developed to physically locate the HSV-1 gene(s) which restore neurovirulence to a non-neurovirulent HSV intertypic recombinant described in the preceding report. In brief, tissue culture cells are co-transfected with unit length RE6 DNA and restriction endonuclease fragmented HSV-...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90544-5
更新日期:1983-11-01 00:00:00
abstract::Hantaviruses are enveloped RNA viruses that belong to the family Bunyaviridae. They are the causative agents of hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Hantaviruses show a worldwide distribution with specific rodent species as natural hosts. It is known that rodents can tr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.09.006
更新日期:2004-01-05 00:00:00
abstract::Transgenic pigs are currently the most favored potential source of organs for xenotransplantation. Like all mammalian species they all harbor endogenous retroviruses in their genome. These porcine endogenous retroviruses (PERVs) are produced from several primary cells and cell lines and are able to infect human cells....
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0149
更新日期:2000-03-01 00:00:00
abstract::Cucumber seedlings infected with cucumber mosaic virus (CMV) contain a virus-induced RNA-dependent RNA polymerase (RNA replicase) in both soluble and particulate fractions of plant extracts; no such activity is found in uninfected plants. The extensive purification and characterization of the soluble RNA replicase has...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90131-8
更新日期:1981-08-01 00:00:00
abstract::The early steps of Rous sarcoma virus-Bryan strain (BS-RSV) infection of genetically resistant and susceptible chick embryo cells were investigated. The methods used were the uptake of virus by cells grown in monolayer, and the ability of infected cells to form foci of transformed cells after plating on resistant and ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(67)90046-3
更新日期:1967-08-01 00:00:00
abstract::The Epstein-Barr virus (EBV) predominantly establishes a latent infection in B lymphocytes, and occasionally switches from the latent state to the lytic cycle. In this report, we identified and examined the role of a lytic gene, BRRF2. We first prepared an antibody against BRRF2 and identified the gene product as a vi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.05.010
更新日期:2015-10-01 00:00:00
abstract::RNase protection analysis of mRNAs for Epstein-Barr virus (EBV) genes activated early in the induction of the productive cycle in Akata cells has confirmed that BZLF1 and BRLF1 behave as immediate early genes. These are likely to be the only virus immediate early genes since analysis of three other transcripts previou...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1070
更新日期:1993-02-01 00:00:00
abstract::Primate lentiviruses are composed of several distinct lineages, including human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus SIVagm. HIV-1 and HIV-2 have significant differences in the mechanisms of viral RNA encapsidation. Therefore, the RNA packaging mechanisms of SIVagm cannot be ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.01.030
更新日期:2007-06-20 00:00:00
abstract::A major 135-kDa DNA binding protein (mDBP) encoded by the BALF2 open reading frame of Epstein-Barr Virus (EBV) is known to be an essential protein for the induction of the lytic cycle. The present investigation was carried out to know whether this protein forms a complex in vivo with other viral DNA binding proteins (...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8891
更新日期:1997-12-22 00:00:00
abstract::We have examined the host range of AIDS-associated retroviruses (ARV) that are known to infect human T cells of the helper subset. We have observed that the virus cannot infect fibroblast and epithelial cell lines of many different animal species. It is infectious and replicates efficiently in peripheral mononuclear c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90146-1
更新日期:1985-12-01 00:00:00
abstract::We describe studies of woodchuck hepatitis virus nucleic acids in liver and other tissues of chronically infected woodchucks, using Southern and Northern blot hybridization techniques. Single-stranded and covalently closed circular replicative DNA molecules were distinguished from partly double-stranded virus genomes....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90035-4
更新日期:1989-03-01 00:00:00
abstract::It is well established that +ssRNA viruses manipulate cellular lipid homoeostasis and distribution to facilitate efficient replication. Here, we show that the cellular lipid ceramide is redistributed to the West Nile virus strain Kunjin virus (WNVKUN) replication complex (RC) but not to the dengue virus serotype 2 str...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.06.015
更新日期:2015-10-01 00:00:00
abstract::Loading of most endogenous peptides on major histocompatibility complex class I molecules is conditional on their transport into the endoplasmic reticulum (ER) by the peptide transporter TAP. We describe an HSV-2/1 cross-reactive cytotoxic T-cell (CTL) epitope that is processed in a TAP1-independent manner in vivo fol...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0829
更新日期:2001-03-30 00:00:00