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Genotype-specific synthesis and secretion of spliced hepatitis B virus genomes in hepatoma cells.

Abstract：

:Hepatitis B virus-infected patients frequently have viral particles with DNA derived from differently spliced RNA. Which factors influence the synthesis of these splice variants is unclear. We analysed the type of splice variants produced from different genotypes and determined whether they are secreted as efficiently as wild-type virus. We demonstrate production of a single splice variant from genotypes D, C, and E as dominant species in two hepatoma cell lines. The type of minor splice variants synthesised varied between genotypes but was identical in both hepatoma cell lines. A novel splice variant with a deletion in the core gene was identified for genotype D. Viral DNA from intracellular compared with extracellular viral particles was spliced approximately five times more often than wild-type-sized genomes. A variable amount of the major splice variant was also identified in sera from patients infected with genotypes A, D, and C. These data indicate genotype A-, C-, D-, and E- as well as hepatoma cell line-independent synthesis of a dominant single splice variant and argue for a biological function of the corresponding splice sites. This study clearly demonstrates the intracellular accumulation of viral particles containing spliced genomes and offers a tool for the investigation of underlying mechanisms.

journal_name

Virology

journal_title

Virology

authors

Sommer G,van Bömmel F,Will H

doi

10.1006/viro.2000.0331

subject

Has Abstract

pub_date

2000-06-05 00:00:00

pages

371-81

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(00)90331-3

journal_volume

271

pub_type

杂志文章

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