Abstract:
:Two ribozyme genes were designed to cut within the VP4 and VP3 sequences of foot and mouth disease virus (FMDV) Asia1 serotype genome. The two genes were synthesized and cloned into pBluescript under the control of the T3 promoter. The ribozyme designed to cut the VP4 gene contained two catalytic sequences targeted to two GUC triplets that are 16 bases apart. The second ribozyme, intended to cut VP3, contained one catalytic sequence. Ribozymes obtained from run-off transcription from both plasmids were able to cleave viral RNA derived from runoff transcripts of plasmids carrying the proper FMDV cDNA inserts. The significance of these findings is discussed.
journal_name
Virus Genesjournal_title
Virus genesauthors
Stram Y,Molad Tdoi
10.1007/BF01702658subject
Has Abstractpub_date
1995-01-01 00:00:00pages
155-9issue
2eissn
0920-8569issn
1572-994Xjournal_volume
9pub_type
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