Abstract:
:Brazil is one of the largest silk producers in the world. The domesticated silkworm (Bombyx mori) was formally introduced into the country in the twentieth century, and the state of Paraná is the main national producer. During larval stages, B. mori can be afflicted by many different infectious diseases, which lead to substantial losses in silk production. In this work, we describe the structure and complete genome sequence of the first non-Asian isolate of Bombyx mori nucleopolyhedrovirus (BmNPV), the most important silkworm pathogen. The BmNPV-Brazilian isolate is a nucleopolyhedrovirus with singly enveloped nucleocapsids within polyhedral occlusion bodies. Its genome has 126,861 bp with a G + C content of 40.4 %. Phylogenetic analysis clustered the virus with the Japanese strain (BmNPV-T3). As expected, we have detected intra-population variability in the virus sample. Variation along homologous regions (HRs) and bro genes was observed; there were seven HRs, deletion of bro-e, and division of bro-a into two ORFs. The study of baculoviruses allows for a better understanding of virus evolution providing insight for biological control of insect pests or protection against the pernicious disease caused by these viruses.
journal_name
Virus Genesjournal_title
Virus genesauthors
Ardisson-Araújo DM,Melo FL,de Souza Andrade M,Brancalhão RM,Báo SN,Ribeiro BMdoi
10.1007/s11262-014-1112-6subject
Has Abstractpub_date
2014-12-01 00:00:00pages
477-84issue
3eissn
0920-8569issn
1572-994Xjournal_volume
49pub_type
杂志文章相关文献
VIRUS GENES文献大全abstract::Bats are carriers of potentially zoonotic viruses, therefore it is crucial to identify viruses currently found in bats to better understand how they are maintained in bat populations and evaluate risks for transmission to other species. Adenoviruses have been previously detected in bats throughout the world, but sampl...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-019-01719-2
更新日期:2020-02-01 00:00:00
abstract::The broad distribution and prevalence of H3 subtype influenza viruses in avian and mammalian hosts constitutes a global threat to both human and veterinary health. In this present study, six H3N8 influenza viruses isolated from domestic ducks during 2004-2005 in northern China were genetically and phylogenetically cha...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-008-0300-7
更新日期:2009-02-01 00:00:00
abstract::White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. The viral early genes play a key role in DNA replication and virus proliferation. In this study, a WSSV gene (wsv477) encoding 208 amino acid peptides was characterized as an early gene. The temporal analysis showe...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-006-0053-0
更新日期:2007-04-01 00:00:00
abstract::The genetic diversity of classical swine fever virus (CSFV) was studied by RT-PCR amplification and sequencing of a 409 bp fragment of the NS5B polymerase region. A total of 106 viruses isolated from 20 countries over a period of 52 years (1945-1997) were included in the phylogenetic study. The results showed that the...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008132613228
更新日期:1999-01-01 00:00:00
abstract::The 5(-terminal leader sequence of the equine arteritis virus (EAV) genome contains an open reading frame (ORF) with an AUG codon in a suboptimal context for initiation of protein synthesis. To investigate the significance of this intraleader ORF (ILO), an expression plasmid was generated carrying a DNA copy of the su...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-005-0030-z
更新日期:2006-08-01 00:00:00
abstract::Chinese equine infectious anemia virus (EIAV) attenuated vaccine is the first lentiviral vaccine with a successful application. In order to understand the correlation of viral genomic mutations with viral attenuation and with induced immunoprotective properties, we analyzed the proviral genome sequences of the EIAV-at...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-010-0491-6
更新日期:2010-08-01 00:00:00
abstract::In this study, a novel duplex nanoparticle-assisted polymerase chain reaction (nanoPCR) assay was developed to detect porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV). Two pairs of primers were designed based on the conserved region within the N gene of PEDV and TGEV. In a ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-016-1405-z
更新日期:2017-02-01 00:00:00
abstract::Astroviruses (AstV) are associated with enteric and systemic disease in mammals and birds. Astroviruses have received increased attention recently as they have been found to be associated with sporadic neurologic disease in mammals including humans. In pigs, porcine astrovirus (PoAstV) can be widely detected and has b...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-019-01692-w
更新日期:2019-10-01 00:00:00
abstract::A goose parvovirus (GPV) Y strain was isolated from Muscovy ducks in Anhui Province of China. By polymerase chain reaction method, its complete genomic sequence was found to be 5,106 bp in length, consisting of 444-bp inverted terminal repeat, 1,844-bp non-structural protein and 2,199-bp capsid protein (VP) regions. T...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-1001-4
更新日期:2014-02-01 00:00:00
abstract::Canine distemper (CD) is a highly contagious, often fatal, multisystemic, and incurable disease in dogs and other carnivores, which is caused by canine distemper virus (CDV). Although vaccines have been used as the principal means of controlling the disease, CD has been reported in vaccinated animals. The hemoagglutin...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-014-1062-z
更新日期:2014-08-01 00:00:00
abstract::The main function of baculoviral chitinase protein (V-CHIA) is to promote the final liquefaction of infected host larvae, facilitating the dispersion of occlusion bodies (OBs) in the environment. In this study, a v-chiA from Epinotia aporema Granulovirus (EpapGV) was identified and characterized. The 1,713 base pairs ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-1019-7
更新日期:2014-04-01 00:00:00
abstract::A novel fluorescent probe-based real-time reverse transcription recombinase polymerase amplification (real-time RT-RPA) assay was developed for rapid detection of highly pathogenic type 2 porcine reproductive and respiratory syndrome virus (HP-PRRSV). The sensitivity analysis showed that the detection limit of RPA was...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-016-1378-y
更新日期:2016-12-01 00:00:00
abstract::Two ribozyme genes were designed to cut within the VP4 and VP3 sequences of foot and mouth disease virus (FMDV) Asia1 serotype genome. The two genes were synthesized and cloned into pBluescript under the control of the T3 promoter. The ribozyme designed to cut the VP4 gene contained two catalytic sequences targeted to...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF01702658
更新日期:1995-01-01 00:00:00
abstract::Complementary DNAs (cDNAs) to the RNA genome of a necrotic strain of potato virus Y in Japan (Hokkaido Univ. isolate of PVY-T:PVY-TH) were synthesized and cloned into a plasmid pBR322. About 4.3 kbp of the cDNA sequence containing the 3'-poly(A) tract of PVY-TH was inserted into a recombinant plasmid pBRYT88. The coat...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00570028
更新日期:1990-12-01 00:00:00
abstract::The nucleotide sequence of two cloned restriction fragments encompassing the granulin genes from the granuloviruses of the potato tuber moth, Phthorimaea operculella, PhopGV, and the Egyptian cotton leaf worm, Spodoptera littoralis, SpliGV, have been determined. Although both viruses are able to infect the same Ph. op...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008179228236
更新日期:2000-10-01 00:00:00
abstract::N20-RNA 3L, a large form of RNA 3 associated with Alfalfa mosaic virus (AMV) strain N20 comprises 2281 nt and has approximately 97% overall sequence similarity to the longest previously described RNA 3 of AMV strain YSMV (YSMV-RNA 3; 2188 nt). Compared with YSMV-RNA 3, N20-RNA 3L contains an additional 97 nt in the 5'...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-004-7433-0
更新日期:2004-12-01 00:00:00
abstract::We report here the complete genome sequence of a bipartite virus, herein denoted WLPRV/human/BRA/TO-34/201, from a sample collected in 2015 from a two-year-old child in Brazil presenting acute gastroenteritis. The virus has 98-99% identity (segments 2 and 1, respectively) with the Wuhan large pig roundworm virus (uncl...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-018-1557-0
更新日期:2018-06-01 00:00:00
abstract::The Chinese equine infectious anemia virus (EIAV) virulent strain EIAV(LN40) is derived from a naturally occurring virus by continuously passing in horses for 16 generations. Its genome sequence is 23% different from that of the American strains or the Japanese strains, and the variation of envelope gp90 surface unit ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-010-0563-7
更新日期:2011-04-01 00:00:00
abstract::Emerging pseudorabies virus (PRV) variant has led to frequent outbreaks of PRV infection among Bartha-K61-vaccinated swine population in Chinese swine farms and caused high mortality in pigs of all age since late 2011. Here, we generated a gE/gI-deleted PRV (rPRVXJ-delgI/gE-EGFP) based on PRV variant strain (PRV-XJ) t...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-017-1454-y
更新日期:2017-08-01 00:00:00
abstract::Sacbrood virus (SBV) represents a serious threat to the health of managed honeybees. We determined four complete SBV genomic sequences (AmSBV-Kor1, AmSBV-Kor2, AcSBV-Kor3, and AcSBV-Kor4) isolated from Apis mellifera and Apis cerana in various regions of South Korea. A phylogenetic tree was constructed from the comple...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-015-1268-8
更新日期:2016-04-01 00:00:00
abstract::India is endemic for foot-and-mouth disease (FMD) and in recent years a unique group within serotype A, carrying a codon deletion at an antigenically critical site in capsid protein VP3 has emerged (VP3(59)-deletion group). This tempted us to analyze the noncoding region, which is an under represented area, though cri...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0366-x
更新日期:2009-08-01 00:00:00
abstract::A bovine viral diarrhea disease virus (BVDV) GS-4 was isolated in Western China form dairy cattle with respiratory disease. Genomic comparison analysis with the 5' half genome sequence encompassing the coding region of N(pro), capsid, and envelope glycoproteins showed that the GS-4 should be classified into BVDV-1b1, ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-0917-z
更新日期:2013-08-01 00:00:00
abstract::DNA viruses as their host cells require a DNA-dependent DNA polymerase (Pol) to faithfully replicate their genomic information. Large eukaryotic DNA viruses as well as bacterial viruses encode a specific Pol equipped with a proofreading 3'-5'-exonuclease, and other replication proteins. All known viral Pol belong to f...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1007997609122
更新日期:1998-01-01 00:00:00
abstract::The highly pathogenic Bombyx mori nucleopolyhedrovirus (BmNPV) has caused severe damages to sericulture in many countries, and the relationship between the pathogenicity of various BmNPV strains and their geographical evolution has been the topic of our interest. In this study, we isolated a new BmNPV strain from Thai...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-012-0721-1
更新日期:2012-06-01 00:00:00
abstract::Bovine viral diarrhea virus (BVDV) belongs to the Pestivirus genus of the Flaviviridae family and has worldwide distribution, being one of the main causes of economic losses in cattle raising. The genome of pestiviruses is a single strand of positive-sense RNA with a length of 12.3 kb, which encodes one open reading f...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-020-01756-2
更新日期:2020-08-01 00:00:00
abstract::Orf virus (ORFV), a typical member of the Parapoxvirus genus within the family Poxviridae, which is the causative agent of Orf, a common epitheliotropic viral disease of sheep, goats, wild ruminants, and humans. In the present study, we sequenced the complete genomic sequences of two ORFV strains (ORFV-SY17, isolated ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-019-01666-y
更新日期:2019-08-01 00:00:00
abstract::In 2009, a bovine parainfluenza virus (BPIV3), named as NM09, was isolated using MDBK cell culture from the nasal swabs of normal cattle in China. The NM09 isolate was characterized by RT-PCR and nucleotide sequence analysis. Its complete genome was 15,456 nucleotides in length. Similar to other sequenced PIV strains,...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-012-0810-1
更新日期:2012-12-01 00:00:00
abstract::Mokola virus, one of the six genotypes within the Lyssavirus genus of the Rhabdoviridae family, is believed to be exclusive to the African continent, where infections in various mammal species have been reported. After an isolation of Mokola virus at Umhlanga on the east coast of South Africa in 1970, the virus was no...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008120511752
更新日期:2000-01-01 00:00:00
abstract::Cytotoxic T cells that recognize dengue fever viral (DFV) peptides were reported. To predict the cleavage pattern of DFV proteins by cytoplasmic proteasomes into nonapeptides with motifs fitting known HLA class I molecules, the computer program "Findpatterns" was used. In this study the combined amino acid motifs for ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF01701808
更新日期:1995-01-01 00:00:00
abstract::The spontaneous host-range mutants 812F1 and K1/420 are derived from polyvalent phage 812 that is almost identical to phage K, belonging to family Myoviridae and genus Kayvirus. Phage K1/420 is used for the phage therapy of staphylococcal infections. Endolysin of these mutants designated LysF1, consisting of an N-term...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-017-1507-2
更新日期:2018-02-01 00:00:00