Abstract:
:A novel fluorescent probe-based real-time reverse transcription recombinase polymerase amplification (real-time RT-RPA) assay was developed for rapid detection of highly pathogenic type 2 porcine reproductive and respiratory syndrome virus (HP-PRRSV). The sensitivity analysis showed that the detection limit of RPA was 70 copies of HP-PRRSV RNA/reaction. The real-time RT-RPA highly specific amplified HP-PRRSV with no cross-reaction with classic PRRSV, classic swine fever virus, pseudorabies virus, and foot-and-mouth disease virus. Assessment with 125 clinical samples showed that the developed real-time RT-RPA assay was well correlated with real-time RT-qPCR assays for detection of HP-PRRSV. These results suggest that the developed real-time RT-RPA assay is suitable for rapid detection of HP-PRRSV.
journal_name
Virus Genesjournal_title
Virus genesauthors
Yang Y,Qin X,Sun Y,Chen T,Zhang Zdoi
10.1007/s11262-016-1378-ysubject
Has Abstractpub_date
2016-12-01 00:00:00pages
883-886issue
6eissn
0920-8569issn
1572-994Xpii
10.1007/s11262-016-1378-yjournal_volume
52pub_type
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