Rapid detection of highly pathogenic porcine reproductive and respiratory syndrome virus by a fluorescent probe-based isothermal recombinase polymerase amplification assay.

Abstract:

:A novel fluorescent probe-based real-time reverse transcription recombinase polymerase amplification (real-time RT-RPA) assay was developed for rapid detection of highly pathogenic type 2 porcine reproductive and respiratory syndrome virus (HP-PRRSV). The sensitivity analysis showed that the detection limit of RPA was 70 copies of HP-PRRSV RNA/reaction. The real-time RT-RPA highly specific amplified HP-PRRSV with no cross-reaction with classic PRRSV, classic swine fever virus, pseudorabies virus, and foot-and-mouth disease virus. Assessment with 125 clinical samples showed that the developed real-time RT-RPA assay was well correlated with real-time RT-qPCR assays for detection of HP-PRRSV. These results suggest that the developed real-time RT-RPA assay is suitable for rapid detection of HP-PRRSV.

journal_name

Virus Genes

journal_title

Virus genes

authors

Yang Y,Qin X,Sun Y,Chen T,Zhang Z

doi

10.1007/s11262-016-1378-y

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

883-886

issue

6

eissn

0920-8569

issn

1572-994X

pii

10.1007/s11262-016-1378-y

journal_volume

52

pub_type

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