Abstract:
:In this study, a novel duplex nanoparticle-assisted polymerase chain reaction (nanoPCR) assay was developed to detect porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV). Two pairs of primers were designed based on the conserved region within the N gene of PEDV and TGEV. In a screening of 114 clinical samples from four provinces in China for PEDV and TGEV, 48.2 and 3.5 % of the samples, respectively, tested positive. Under optimized conditions, the duplex nanoPCR assay had a detection limit of 7.6 × 101 and 8.5 × 101 copies μL-1 for PEDV and TGEV, respectively. The sensitivity of the duplex nanoPCR assay was ten times higher than that of a conventional PCR assay. Moreover, no fragments were amplified when the duplex nanoPCR assay was used to test samples containing other porcine viruses. Our results indicate that the duplex nanoPCR assay described here is useful for the rapid detection of PEDV and TGEV and can be applied in clinical diagnosis.
journal_name
Virus Genesjournal_title
Virus genesauthors
Zhu Y,Liang L,Luo Y,Wang G,Wang C,Cui Y,Ai X,Cui Sdoi
10.1007/s11262-016-1405-zsubject
Has Abstractpub_date
2017-02-01 00:00:00pages
71-76issue
1eissn
0920-8569issn
1572-994Xpii
10.1007/s11262-016-1405-zjournal_volume
53pub_type
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