Abstract:
:Boid inclusion body disease (BIBD) is a viral disease of boids caused by reptarenavirus. In this study, tissue from naturally infected boid snakes were homogenized and propagated in African Monkey kidney (Vero) and rat embryonic fibroblast (REF) cells. Virus replication was determined by the presence of cytopathic effect, while viral morphology was observed using transmission electron microscopy. Viral RNA was amplified using RT-PCR with primers specific for the L-segment of reptarenavirus; similarly, quantification of viral replication was done using qPCR at 24-144 h postinfection. Viral cytopathology was characterized by cell rounding and detachment in both Vero and REF cells. The viral morphology showed round-to-pleomorphic particles ranging from 105 to 150 nm which had sand-like granules. Sanger sequencing identified four closely associated reptarenavirus species from 15 (37.5 %) of the total samples tested, and these were named as follows: reptarenavirus UPM-MY 01, 02, 03, and 04. These isolates were phylogenetically closely related to the University Helsinki virus (UHV), Boa Arenavirus NL (ROUTV; BAV), and unidentified reptarenavirus L20 (URAV-L20). Comparison of deduced amino acid sequences further confirmed identities to L-protein of UHV, L-polymerase of BAV and RNA-dependent RNA polymerase of URAV-L20. Viral replication in Vero cells increased steadily from 24 to 72 h and peaked at 144 h. This is the first study in South East Asia to isolate and characterize reptarenavirus in boid snakes with BIBD.
journal_name
Virus Genesjournal_title
Virus genesauthors
Abba Y,Hassim H,Hamzah H,Ibrahim OE,Ilyasu Y,Bande F,Mohd Lila MA,Noordin MMdoi
10.1007/s11262-016-1345-7subject
Has Abstractpub_date
2016-10-01 00:00:00pages
640-50issue
5eissn
0920-8569issn
1572-994Xpii
10.1007/s11262-016-1345-7journal_volume
52pub_type
杂志文章相关文献
VIRUS GENES文献大全abstract::Varicella-zoster virus (VZV) DNA was detectable by in-situ hybridization in blood mononuclear cells (MNCs) of patients with varicella or zoster for 2-56 days after the onset of a rash. VZV DNA was present in many MNCs from one acute varicella patient 2 days after the onset of the rash and was rarely found in MNCs duri...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00684037
更新日期:1989-08-01 00:00:00
abstract::Viroids, the smallest and simplest agents of infectious disease, cause a number of economically important diseases of crop plants. Present evidence indicates that most of these diseases originated recently (in the 20th century) by chance transfer of viroids from endemically infected wild plants or by use of viroid-inf...
journal_title:Virus genes
pub_type: 杂志文章,评审
doi:10.1007/BF01728653
更新日期:1995-01-01 00:00:00
abstract::Human T-cell leukemia virus type 1 (HTLV-1) is a causative agent of adult T-cell leukemia. HTLV-1 Tax1 transforming protein interacts with several PDZ domain-containing proteins, and the interaction is associated with the transforming activities of Tax1 as well as persistent HTLV-1 infection. In this study, we show th...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-008-0259-4
更新日期:2008-10-01 00:00:00
abstract::The intraspecies variability of capsid proteins of five viruses of the genus Comovirus was established. Inclusion of both capsid proteins to the sequence analysis reduces some uncertainties about species/strains demarcation criteria in the Comovirus genus. New approach is proposed for discrimination of Turnip ringspot...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0443-1
更新日期:2010-04-01 00:00:00
abstract::The nucleic acid and deduced amino acid sequence of the fibre gene of the HNF-61 strain of porcine adenovirus serotype 5 (PAdV-5) was determined and compared to that of the HNF-70 strain of the same serotype (Nagy et al., J Gen Virol 82, 525-529, 2001) and also to adenovirus fibre genes from the genera Mastadenovirus ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1014580802250
更新日期:2002-03-01 00:00:00
abstract::Recent studies indicate that alphaherpesviruses express latency associated transcripts (LATs) from the antisense strand of immediate early (IE) genes of the viral genome. It has been discussed that LATs containing extended open reading frames (ORFs) might be translated into protein products. We found that a salient fe...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF01703434
更新日期:1994-09-01 00:00:00
abstract::In 2009, a bovine parainfluenza virus (BPIV3), named as NM09, was isolated using MDBK cell culture from the nasal swabs of normal cattle in China. The NM09 isolate was characterized by RT-PCR and nucleotide sequence analysis. Its complete genome was 15,456 nucleotides in length. Similar to other sequenced PIV strains,...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-012-0810-1
更新日期:2012-12-01 00:00:00
abstract::A 13 amino acid residue insertion was found in the N-terminal region of the coat protein of several Maize dwarf mosaic virus isolates (MDMV). These insertions seem to be the result of a direct duplication event, but differ in some positions. In order to evaluate the influence of the insertion on the RNA secondary stru...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0425-3
更新日期:2010-02-01 00:00:00
abstract::A low-cost rodent model of HIV infection and which presents high application value is an effective tool to investigate HIV infection and pathogenesis. However, development of such a small animal model has been hampered by the unsuitability of rodent cells for HIV-1 replication given that the retrovirus HIV-1 has high ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-016-1423-x
更新日期:2017-04-01 00:00:00
abstract::The 5(-terminal leader sequence of the equine arteritis virus (EAV) genome contains an open reading frame (ORF) with an AUG codon in a suboptimal context for initiation of protein synthesis. To investigate the significance of this intraleader ORF (ILO), an expression plasmid was generated carrying a DNA copy of the su...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-005-0030-z
更新日期:2006-08-01 00:00:00
abstract::Two restriction fragments of bovine papillomavirus type 5 (BPV5), of a genomic size of 1.6 and 1.2 kb were subcloned and sequenced. One of them seemed to correspond to the 3' end of the E1 open reading frame (ORF) and the other to the region of the E7, E8 and 5' end of the E1 ORF. Alignments of these fragments with ot...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1007960324424
更新日期:1997-01-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome (PRRS) is considered to be one of the most important infectious diseases affecting livestock. This study used gene sequence analysis of ORF5 and Nsp2 to determine the molecular epidemiology of PRRSV in different parts of the Guangxi province of China. These genes were sele...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-012-0824-8
更新日期:2013-02-01 00:00:00
abstract::Foot-and-mouth disease (FMD) is the most contagious and devastating disease of livestock. Our previous studies demonstrated that TK(-)/gG(-)/P1(+), a recombinant expressing the FMDV capsid precursor protein (P1) based on attenuated pseudorabies virus (PRV) TK(-)/gG(-), could be used as a recombinant vaccine to protect...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-008-0204-6
更新日期:2008-04-01 00:00:00
abstract::The highly pathogenic Bombyx mori nucleopolyhedrovirus (BmNPV) has caused severe damages to sericulture in many countries, and the relationship between the pathogenicity of various BmNPV strains and their geographical evolution has been the topic of our interest. In this study, we isolated a new BmNPV strain from Thai...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-012-0721-1
更新日期:2012-06-01 00:00:00
abstract::Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) ORF39 (designated as the bjdp gene) is the only J domain gene recorded in baculovirus genomes to date. Computer-assisted analysis revealed that its deduced amino acid sequences possessed a coiled-coil region and a RNA recognition motif (RRM). In vivo the bd...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1020980109450
更新日期:2002-12-01 00:00:00
abstract::African monkeys are resistant to HIV-1 infection due to intrinsic restriction mechanisms found in their cells. However, although they can be infected by monkey-adapted modified HIV-1 particles that are designed to overcome known restriction factors, virus numbers drop to undetectable levels in immunocompetent animals....
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-020-01731-x
更新日期:2020-04-01 00:00:00
abstract::Merkel cell carcinoma (MCC) is a highly aggressive neuroendocrine skin cancer associated with the Merkel cell polyomavirus (MCPyV). The MCPyV genome, which is clonally integrated in the majority of MCCs, encodes the regulatory small T (sT) antigen. Previously, reports have established MCPyV sT antigen as a potent onco...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-016-1304-3
更新日期:2016-06-01 00:00:00
abstract::A BK virus isolate cloned from the urine of an immunosuppressed patient was identified as the WW strain. Fine mapping using four base-pair restriction enzymes identified a number of small deletions and insertions relative to prototype BK. The finding of a transcriptional control region with a structure similar to that...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00315259
更新日期:1989-03-01 00:00:00
abstract::Tumor-selectively replicating (oncolytic) viruses are promising tools for therapy of solid cancers and have been initially developed to achieve potent tumor lysis with acceptable side effects on healthy tissue. However, in recent years, oncolytic viruses have been recognized as therapeutic vehicles exhibiting multipro...
journal_title:Virus genes
pub_type: 杂志文章,评审
doi:10.1007/s11262-017-1488-1
更新日期:2017-10-01 00:00:00
abstract::Sacbrood virus (SBV) represents a serious threat to the health of managed honeybees. We determined four complete SBV genomic sequences (AmSBV-Kor1, AmSBV-Kor2, AcSBV-Kor3, and AcSBV-Kor4) isolated from Apis mellifera and Apis cerana in various regions of South Korea. A phylogenetic tree was constructed from the comple...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-015-1268-8
更新日期:2016-04-01 00:00:00
abstract::Influenza A viruses infect a range of host species, including various mammals and more than 100 species of birds. For avian influenza viruses (AIV), prevalence varies between different groups of birds, with waterfowl showing the highest prevalence. We have sequenced the complete genome of A/Thick-billed Murre/Newfound...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-010-0504-5
更新日期:2010-10-01 00:00:00
abstract::The Kansas/15626/84 (Ka) and Shipping Fever (SF) strains of bovine parainfluenza virus type 3 (BPIV3) replicate less efficiently than human PIV3 (HPIV3) in the upper and lower respiratory tract of rhesus monkeys, and BPIV3 Ka is also highly attenuated in humans and is in clinical trials as a candidate vaccine against ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008130917204
更新日期:2000-01-01 00:00:00
abstract::Twenty-four avian influenza viruses were collected from poultry farms in three different governorates in Egypt during the years 2006-2009 and genetically characterized. All the isolates were confirmed to be type A and subtype H5 influenza virus by chromatographic strip test and hemagglutination inhibition assay. The s...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-011-0633-5
更新日期:2011-10-01 00:00:00
abstract::Bacteriophage T7 RNA polymerase and a derivative containing a nuclear localization signal were transiently expressed in CV-1 cells and were shown to localize to the cytoplasm and nucleus, respectively. A vector was constructed containing T7 promoter and transcription terminator sequences flanking a picornaviral 5' unt...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF01702562
更新日期:1992-08-01 00:00:00
abstract::The antigenic variation in Foot and Mouth Disease Virus (FMDV) is very high. The effective strategy to control the Foot and Mouth Disease (FMD) in India which is a habitat of four serotypes O, A, C and Asia 1, is by regular vaccination, using the vaccine strain most suitable for the local situation. India is an endemi...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1007984621839
更新日期:1997-01-01 00:00:00
abstract::Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) odv-e25 is 684 bp long, potentially encoding 227 amino acids with a predicted molecular weight of 24.9 kDa. Homology analysis indicated that SpltMNPV ODV-E25 has 35-65% amino acid identity with that of other known baculoviruses. RT-PCR results revealed that...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-005-5840-5
更新日期:2006-02-01 00:00:00
abstract::A novel fluorescent probe-based real-time reverse transcription recombinase polymerase amplification (real-time RT-RPA) assay was developed for rapid detection of highly pathogenic type 2 porcine reproductive and respiratory syndrome virus (HP-PRRSV). The sensitivity analysis showed that the detection limit of RPA was...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-016-1378-y
更新日期:2016-12-01 00:00:00
abstract::Infection of infants in their first year of life, children and elderly people with the respiratory syncytial virus (RSV) endangers the life of the patient. An attempt to develop a formalin-inactivated RSV (FI-RSV) vaccine during the 1960s resulted in an aggravated infection in immunized children, leading to hospitaliz...
journal_title:Virus genes
pub_type: 杂志文章,评审
doi:10.1007/s11262-006-0064-x
更新日期:2006-10-01 00:00:00
abstract::Hepatitis E virus (HEV) has emerged as an important cause of epidemic and sporadic acute viral hepatitis worldwide, which is a major public health challenge. A better understanding of the interaction between the virus and the host cell would be very helpful for its therapy. Swine HEV (SHEV) open reading frame 3 (ORF3)...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-0912-4
更新日期:2013-08-01 00:00:00
abstract::Porcine circovirus 2 (PCV2) can cause porcine circovirus diseases and porcine circovirus-associated diseases (PCVD/PCVAD), which are widely presented in swine-producing countries. Since the discovery of this virus, considerable efforts have been devoted to understanding this pathogen and its interactions with its host...
journal_title:Virus genes
pub_type: 杂志文章,评审
doi:10.1007/s11262-016-1326-x
更新日期:2016-08-01 00:00:00