Abstract：
:Porcine reproductive and respiratory syndrome (PRRS) is considered to be one of the most important infectious diseases affecting livestock. This study used gene sequence analysis of ORF5 and Nsp2 to determine the molecular epidemiology of PRRSV in different parts of the Guangxi province of China. These genes were selected due to their extensive variation within the genome. Out of 189 samples from animals suspected to have PRRS, 145 were PRRSV RNA positive. ORF5 and Nsp2 gene sequence analysis of 31 of these samples showed that all of the Guangxi isolates were of type 2. A phylogenetic tree analysis based on ORF5 showed that the Guangxi isolates were divided into two groups. Most of these were closely related to highly pathogenic strains, showing a 30 amino acid deletion at positions 481 and 533-561 of Nsp2, but an additional unique isolate (GXNN06) possessed a further four amino acid deletion at positions 485-488 of Nsp2.
journal_name
Virus Genesjournal_title
Virus genesauthors
Zhang HY,Liang JJ,Meng XM,Li H,Yang J,Su LJ,Zhang HP,Xie LJ,He XX,Li YS,Yin S,Li XQ,Li XN,Luo TRdoi
10.1007/s11262-012-0824-8subject
Has Abstractpub_date
2013-02-01 00:00:00pages
71-80issue
1eissn
0920-8569issn
1572-994Xjournal_volume
46pub_type
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