Identification and characterization of odv-e25 of Spodoptera litura multicapsid nucleopolyhedrovirus.

Abstract:

:Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) odv-e25 is 684 bp long, potentially encoding 227 amino acids with a predicted molecular weight of 24.9 kDa. Homology analysis indicated that SpltMNPV ODV-E25 has 35-65% amino acid identity with that of other known baculoviruses. RT-PCR results revealed that the odv-e25 is transcribed actively at the late stage of infection and the mRNA start site was mapped within a consensus baculovirus late promoter sequence (TTAAG). Western blot analysis of odv-e25 expression with an antiserum made against 6 x His tagged ODV-E25 expressed in Escherichia coli indicated that it was present as a doublet of approximately 27 kDa from 24 h through 96 h in SpltMNPV-infected Spli-221 cells. Similar results were seen on Western blots of Spodoptera exigua (Se)MNPV-infected Se301 cells. Immunofluorescence analysis showed that ODV-E25 was predominantly present in the cytoplasm of SpltMNPV-infected cells and localized to the envelopes of occlusion-derived virus.

journal_name

Virus Genes

journal_title

Virus genes

authors

Li Z,Pan L,Yu H,Li S,Zhang G,Pang Y

doi

10.1007/s11262-005-5840-5

subject

Has Abstract

pub_date

2006-02-01 00:00:00

pages

13-9

issue

1

eissn

0920-8569

issn

1572-994X

journal_volume

32

pub_type

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