Abstract:
:Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) ORF39 (designated as the bjdp gene) is the only J domain gene recorded in baculovirus genomes to date. Computer-assisted analysis revealed that its deduced amino acid sequences possessed a coiled-coil region and a RNA recognition motif (RRM). In vivo the bdjp gene transcription was detected from 24 h post infection (p.i.) and abundant from 72 to 96 h p.i., while in vitro it was first detected 6 h p.i. and was abundant from 12 to 24 h p.i., respectively. Western blot analysis of BJDP expression with an antiserum made against 6xHis tagged BJDP expressed in Escherichia coli showed that it was present from 24 through 96 h p.i. in vitro. Western blot analysis indicated that BJDP was found to be associated with budded and occlusion-derived virions (ODV). Furthermore, BJDP was shown to be present in both envelope and nucleocapsid fractions of ODV. These results suggested that BJDP might be a structural protein.
journal_name
Virus Genesjournal_title
Virus genesauthors
Wang L,Yu J,Yin C,Li Z,Hu X,Pang Ydoi
10.1023/a:1020980109450subject
Has Abstractpub_date
2002-12-01 00:00:00pages
291-7issue
3eissn
0920-8569issn
1572-994Xpii
5100592journal_volume
25pub_type
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