Abstract:
:Genetic diversity of eight Grapevine leafroll-associated virus 1 (GLRaV-1) isolates recovered from grapevines in three distinct locations in the Czech Republic and Slovakia was characterised by restriction fragment length polymorphism (RFLP) analysis and by sequencing of cloned 540 bp fragment of the heat shock protein 70 (HSP70) gene. Comparison and phylogenetic analysis of obtained and previously available sequence data revealed the existence of two groups of GLRaV-l isolates, tentatively named A and E (genetic divergence between A and E group reached 13.9%). An RT-PCR detection method followed by simple restriction analysis was developed, showing the potential to differentiate GLRaV-1 isolates of these groups. Interestingly, a mixed infection of two GLRaV-1 groups in the same plant was frequently detected together with a high intra-group variability in some isolates.
journal_name
Virus Genesjournal_title
Virus genesauthors
Komínek P,Glasa M,Bryxiová Mdoi
10.1007/s11262-005-3236-1subject
Has Abstractpub_date
2005-12-01 00:00:00pages
247-55issue
3eissn
0920-8569issn
1572-994Xjournal_volume
31pub_type
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