Abstract:
:Pseudorabies virus (PRV) belongs to the Alphaherpesvirinae subfamily of Herpesviridae. PRV-induced pseudorabies is a highly contagious disease that has caused huge economic losses to the global swine industry. The PRV gE/gI gene deletion vaccine strain (Fa ΔgE/gI strain) constructed from the PRV Fa wild-type strain was shown to have a protective effect against infection. However, the interaction between PRV gE/gI genes and host miRNA needs further exploration, and little is known about the regulatory mechanisms of non-coding RNAs during PRV infection. miRNAs play a key regulatory role in viral infection and immune responses, so we analyzed the differential expression of miRNAs induced by the PRV Fa ΔgE/gI strain and Fa wild-type strain in the PK15 cell line. High-throughput sequencing reads were aligned to known Sus scrofa pre-miRNAs in the miRBase database. Target genes of differentially expressed miRNAs were predicted using the miRGen 3.0 database, then filtered miRNA target genes were subjected to Gene Ontology (GO) analysis and Search Tool for the Retrieval of Interacting Genes/ Proteins (STRING) analysis. Stem-loop quantitative real-time PCR was performed to confirm the accuracy of high-throughput sequencing data. In total, 387, 472, and 490 annotated and novel mature miRNAs were identified from PRV Fa ΔgE/gI strain-infected, Fa wild-type strain-infected, and non-infected PK-15 cells, respectively. Five PRV-encoded miRNAs were also identified. GO analysis showed that target genes of differentially expressed miRNAs in PRV Fa ΔgE/gI strain-infected and Fa wild-type strain-infected PK-15 cells were mainly involved in biological regulation and metabolic processes. STRING analysis showed that immune-related target genes of differentially expressed miRNAs in the Toll-like receptor signaling pathway, B cell receptor signaling pathway, T cell receptor signaling pathway, nuclear factor-κB signaling pathway, and transforming growth factor-β signaling pathway were interrelated. This is the first report of the small RNA transcriptome in PRV mutant wild-type strain-infected and Fa ΔgE/gI strain-infected porcine cell lines. Our findings will contribute to the prevention and treatment of PRV mutant strains.
journal_name
Virus Genesjournal_title
Virus genesauthors
Liu X,Zhou Y,Luo Y,Chen Ydoi
10.1007/s11262-020-01760-6subject
Has Abstractpub_date
2020-08-01 00:00:00pages
461-471issue
4eissn
0920-8569issn
1572-994Xpii
10.1007/s11262-020-01760-6journal_volume
56pub_type
杂志文章相关文献
VIRUS GENES文献大全abstract::The reasons for adefovir dipivoxil (ADV) treatment failures appear diverse. Few studies have reported full-length hepatitis B virus (HBV) genome in patients with ADV treatment failures. The patients were from a phase III clinical trial that investigated the antiviral response to ADV in China. Seven patients had increa...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0429-z
更新日期:2010-04-01 00:00:00
abstract::Amino acid and phylogenetic analysis showed that PB1-F2 of H7N7 and H3N8 equine influenza virus (EIV) evolved into two and nine clades, respectively. The PB1-F2 gene of H7N7 EIV strains isolated after 1973 was identified as being replaced by that of H3N8 EIV circulating simultaneously. All H7N7 EIV strains before 1973...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-0935-x
更新日期:2013-10-01 00:00:00
abstract::Proteins harbor domains or short linear motifs, which facilitate their functions and interactions. Finding functional motifs in protein sequences could predict the putative cellular roles or characteristics of hypothetical proteins. In this study, we present Shetti-Motif, which is an interactive tool to (i) map UniPro...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-016-1416-9
更新日期:2017-04-01 00:00:00
abstract::Yellow oat-grass plants (Trisetum flavescens L.) with mild mosaic and pronounced dwarfing symptoms were observed at different locations in the Czech Republic. Electron microscope observations of symptomatic plants revealed the presence of filamentous particles and inclusion bodies characteristic of the family Potyviri...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0373-y
更新日期:2009-08-01 00:00:00
abstract::The Jumonji C-domain containing protein 6 (JMJD6) has had a convoluted history, and recent reports indicating a multifactorial role in foot-and-mouth disease virus (FMDV) infection have further complicated the functionality of this protein. It was first identified as the phosphatidylserine receptor on the cell surface...
journal_title:Virus genes
pub_type: 杂志文章,评审
doi:10.1007/s11262-017-1449-8
更新日期:2017-06-01 00:00:00
abstract::In this study, the relative synonymous codon usage (RSCU) values, effective number of codon (ENC) values, nucleotide contents, and dinucleotide were used to investigate codon usage pattern of each protein-coding gene and genome among 31 Newcastle disease virus (NDV) isolates. The result shows that the overall extent o...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-011-0574-z
更新日期:2011-04-01 00:00:00
abstract::The complete genome sequence of the KGH strain of the first human rabies virus, which was isolated from a skin biopsy of a patient with rabies, whose symptoms developed due to bites from a raccoon dog in 2001. The size of the KGH strain genome was determined to be 11,928 nucleotides (nt) with a leader sequence of 58 n...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-012-0850-6
更新日期:2013-04-01 00:00:00
abstract::Bornaviruses (family Bornaviridae) are non-segmented negative-strand RNA viruses. Avian bornaviruses (ABVs), which are causative agents of proventricular dilatation disease, are a genetically diverse group with at least 15 genotypes, including parrot bornaviruses (PaBVs) and aquatic bird bornavirus 1(ABBV-1). Borna di...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-015-1240-7
更新日期:2015-10-01 00:00:00
abstract::The red imported fire ant (Solenopsis invicta) escaped its natural enemies when it was introduced into North America in the 1930s from South America. US efforts have focused on discovery of natural enemies, like viruses, to provide sustainable control of the ant. Nine new virus genomes were sequenced from the invasive...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-019-01652-4
更新日期:2019-06-01 00:00:00
abstract::A filamentous virus was isolated in Angelica sinensis (Angelica sinensis (Oliv.) Diels) which shows mosaic symptoms on leaves in Minxian, Gansu province, China. According to morphology and molecular biology properties, this virus, which has a flexuous rod-shaped particle about 750 nm in length and 12 nm in width, was ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0361-2
更新日期:2009-08-01 00:00:00
abstract::After 2 minor winter seasons, influenza B viruses were predominantly isolated in the Province of Quebec, Canada, during the 2000-2001 season representing 74% of laboratory-confirmed influenza viruses. We performed an antigenic study of the hemagglutinin (HA) protein and a molecular characterization of the HA1 region, ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1025768308631
更新日期:2003-10-01 00:00:00
abstract::A bovine viral diarrhea disease virus (BVDV) GS-4 was isolated in Western China form dairy cattle with respiratory disease. Genomic comparison analysis with the 5' half genome sequence encompassing the coding region of N(pro), capsid, and envelope glycoproteins showed that the GS-4 should be classified into BVDV-1b1, ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-0917-z
更新日期:2013-08-01 00:00:00
abstract::A low-cost rodent model of HIV infection and which presents high application value is an effective tool to investigate HIV infection and pathogenesis. However, development of such a small animal model has been hampered by the unsuitability of rodent cells for HIV-1 replication given that the retrovirus HIV-1 has high ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-016-1423-x
更新日期:2017-04-01 00:00:00
abstract::The complete nucleotide sequence of genome segment 4 from the human group C rotavirus (Bristol strain) was determined. Comparison of the nucleotide sequences of the genome termini with the consensus 5' and 3' terminal non-coding sequences of the human group C rotavirus genome revealed characteristic 5' and 3' sequence...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00568909
更新日期:1996-01-01 00:00:00
abstract::The genomic structure of two strains of orf virus (OV), a field isolate (MRI-Scab) which has never been passaged in cell culture, and a multiple-passage cell culture-adapted strain (Orf-11) were compared. The Orf-11 genome is approximately 8.0 kb longer than that of the MRI-Scab due to a duplication of the right-hand ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008117127729
更新日期:2001-03-01 00:00:00
abstract::The Tahyna virus (TAHV) is an important human pathogen in the Bunyaviridae family. To date, only the S and M segments of this virus have been sequenced, but the sequence of the L segment hasn't been established yet. In this study, we sequenced 963 nucleotides of the L segment of TAHV, comprising pre-motif A and motif ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-008-0212-6
更新日期:2008-06-01 00:00:00
abstract::Newcastle disease viruses (NDV) isolated from pigeons (pigeon paramyxovirus type 1; PPMV-1) are mostly of mesogenic pathotype and characterized by a polybasic amino acid sequence motif at the fusion protein (F) cleavage site. This feature also applies to isolate R75/98 from Germany. Its genome consists of 15,192 nucle...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-014-1111-7
更新日期:2014-12-01 00:00:00
abstract::In this study, we report the discovery and molecular characterization of a novel mycovirus, Nigrospora oryzae fusarivirus 1 (NoFV1) isolated from the rice-infecting fungus Nigrospora oryzae. Excluding a poly (A) tail, the genome of the virus is 7004 nucleotide (nt) long containing three putative nonoverlapping open re...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-016-1371-5
更新日期:2016-12-01 00:00:00
abstract::The highly pathogenic Bombyx mori nucleopolyhedrovirus (BmNPV) has caused severe damages to sericulture in many countries, and the relationship between the pathogenicity of various BmNPV strains and their geographical evolution has been the topic of our interest. In this study, we isolated a new BmNPV strain from Thai...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-012-0721-1
更新日期:2012-06-01 00:00:00
abstract::Merkel cell carcinoma (MCC) is a highly aggressive neuroendocrine skin cancer associated with the Merkel cell polyomavirus (MCPyV). The MCPyV genome, which is clonally integrated in the majority of MCCs, encodes the regulatory small T (sT) antigen. Previously, reports have established MCPyV sT antigen as a potent onco...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-016-1304-3
更新日期:2016-06-01 00:00:00
abstract::The immunity of a combined DNA vaccine of HSV-2 glycoproteins B2 (gB2) and D2 (gD2) genes in comparison to individual vaccines was studied with regard to protecting against the HSV infection. Two recombinant DNA vaccines of the pHS2-gB2 or pHS2-gD2 were constructed and formulated. The neutralizing antibody titers appe...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1020113902834
更新日期:2002-10-01 00:00:00
abstract::Rice tungro spherical virus (RTSV) was shown to have three coat protein (CP) species by high resolution NaDodSO4-PAGE and Western blot analyses. The sequence of a coat protein-expressing cDNA clone that was identified and selected from a RTSV cDNA library showed that the insert was composed of 2823 bp with only one la...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF01702392
更新日期:1993-06-01 00:00:00
abstract::Several important biological processes are performed by distinct functional domains found on replication-associated protein (Rep) encoded by AC1 of geminiviruses. Two truncated forms of replicase (tAC1) gene, capable of expressing only the N-terminal 669 bp (5'AC1) and C-terminal 783 bp (3'AC1) nucleotides cloned unde...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-011-0569-9
更新日期:2011-04-01 00:00:00
abstract::This study focuses on gene expression of porcine circovirus (PCV) in order to identify viral genes and their corresponding mRNA transcripts. By northern blot analysis, the existence of three mRNAs could be demonstrated. Two mRNAs are encoded by the viral (-)-strand and one is encoded by the viral (+)-strand. The (+)-s...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008022521329
更新日期:1998-01-01 00:00:00
abstract::Twenty-four avian influenza viruses were collected from poultry farms in three different governorates in Egypt during the years 2006-2009 and genetically characterized. All the isolates were confirmed to be type A and subtype H5 influenza virus by chromatographic strip test and hemagglutination inhibition assay. The s...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-011-0633-5
更新日期:2011-10-01 00:00:00
abstract::Adeno-associated virus (AAV) is a human parvovirus that in laboratory and animal models has the ability to suppress the oncogenic phenotype of a variety of viruses and cellular derived oncogenes. The inhibitory effects of AAV have been mapped to its rap gene (Rep78 protein). Furthermore, seroepidemiologic data indicat...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00370000
更新日期:1996-01-01 00:00:00
abstract::A lamb strain of rotavirus has recently been licensed for use in China as a live vaccine to prevent rotavirus diarrhea in children. As rotavirus NSP4, especially the cytotoxic domain alone is considered to be associated with diarrhea, we sequenced gene segment 10, which encodes NSP4, of lamb rotavirus. Comparative ana...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1023491514820
更新日期:2003-01-01 00:00:00
abstract::Staphylococcus aureus is a well-known pathogen that causes several serious diseases in humans and animals. As part of the efforts to control this pathogen, a newly isolated bacteriophage, SA12, which specifically targets S. aureus, was characterized, and its genome was completely sequenced. Host range and bacteriophag...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-0938-7
更新日期:2013-10-01 00:00:00
abstract::To determine the prevalence of TT virus (TTV) and GB virus C/hepatitis G virus (GBV-C) infections among patients with liver disease and the general population in Shanghai, China, we studied 90 patients with liver diseases (acute hepatitis, 28; chronic hepatitis, 27; liver cirrhosis, 20; hepatocellular carcinoma, 15) a...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008188623062
更新日期:1999-01-01 00:00:00
abstract:UNLABELLED:This study determined the complete nucleotide sequence of Beet cryptic virus 1 (BCV1). As expected by analogy to previously sequenced alphacryptoviruses, dsRNA1 (2008 bp) encodes a 72.5-kDa protein containing sequence motifs characteristic for RNA-dependent RNA polymerases (RdRp). In addition to the full-len...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0432-4
更新日期:2010-04-01 00:00:00