Abstract：
:The reasons for adefovir dipivoxil (ADV) treatment failures appear diverse. Few studies have reported full-length hepatitis B virus (HBV) genome in patients with ADV treatment failures. The patients were from a phase III clinical trial that investigated the antiviral response to ADV in China. Seven patients had increase in HBV-DNA (>1 log(10) copies/ml above on-treatment nadir) at week 52. The serum HBV-DNA levels were above 10(4)copies/ml at week 92 in four of them. Sixteen full-length HBV genomes from the four patients at four time points were sequenced using cloning sequencing method. The frequency of substitutions at week 52 was higher than at weeks 28(16 wt) and 92(80). HBV-DNA reduction was correlated negatively with the frequency of substitutions at the three time points. No published ADV-resistant mutations were detected. The mutations, including substitutions in immunogenic epitopes and conserved sites of the polymerase gene, were frequent during ADV treatment. Amino acid deletions in X gene and basal core promoter/pre-core mutations appeared before or during ADV treatment. The substitutions in immunogenic epitopes (mainly of the surface gene) and conserved sites of the polymerase gene other than ADV-resistant mutations may have influenced antiviral efficacy in the study. More potent antiviral drugs may be important to rescue individual patients and for public health safety. It is needed to study how these substitutions influence HBV replication, disease progression, and antiviral treatment efficacy.
journal_name
Virus Genesjournal_title
Virus genesauthors
Li Y,Zhu M,Guo Y,Chen W,Li Gdoi
10.1007/s11262-009-0429-zsubject
Has Abstractpub_date
2010-04-01 00:00:00pages
155-62issue
2eissn
0920-8569issn
1572-994Xjournal_volume
40pub_type
杂志文章相关文献
VIRUS GENES文献大全abstract：:The Kansas/15626/84 (Ka) and Shipping Fever (SF) strains of bovine parainfluenza virus type 3 (BPIV3) replicate less efficiently than human PIV3 (HPIV3) in the upper and lower respiratory tract of rhesus monkeys, and BPIV3 Ka is also highly attenuated in humans and is in clinical trials as a candidate vaccine against ...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1023/a:1008130917204
更新日期：2000-01-01 00:00:00
abstract：:We have extended our studies using the mouse lymphoid cell system to determine if polyomavirus (Py) cell type specificity is a consequence of the simple presence of either the A and B enhancers, or if specificity results from specific combinations or arrangements of the individual enhancer elements. Mouse fibroblasts ...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/BF00125683
更新日期：1987-11-01 00:00:00
abstract：:The spontaneous host-range mutants 812F1 and K1/420 are derived from polyvalent phage 812 that is almost identical to phage K, belonging to family Myoviridae and genus Kayvirus. Phage K1/420 is used for the phage therapy of staphylococcal infections. Endolysin of these mutants designated LysF1, consisting of an N-term...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/s11262-017-1507-2
更新日期：2018-02-01 00:00:00
abstract：:Tobacco bushy top disease is caused by tobacco bushy top virus (TBTV, a member of the genus Umbravirus) which is dependent on tobacco vein-distorting virus (TVDV) to act as a helper virus encapsidating TBTV and enabling its transmission by aphids. Isometric virions from diseased tobacco plants were purified and diseas...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/s11262-010-0524-1
更新日期：2010-12-01 00:00:00
abstract：:The VP2 gene DNA (1.4 kb in approximate) of a very virulent infectious bursal disease virus (vvIBDV) Chinju strain detected in Chinju, Korea was cloned into the bacmid, a baculovirus shuttle vector, through transposition of the gene from initially cloned pFastBacHTa plasmid, a baculovirus expression vector, and was su...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1023/a:1025128619976
更新日期：2003-08-01 00:00:00
abstract：:Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) ORF39 (designated as the bjdp gene) is the only J domain gene recorded in baculovirus genomes to date. Computer-assisted analysis revealed that its deduced amino acid sequences possessed a coiled-coil region and a RNA recognition motif (RRM). In vivo the bd...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1023/a:1020980109450
更新日期：2002-12-01 00:00:00
abstract：:This study focuses on gene expression of porcine circovirus (PCV) in order to identify viral genes and their corresponding mRNA transcripts. By northern blot analysis, the existence of three mRNAs could be demonstrated. Two mRNAs are encoded by the viral (-)-strand and one is encoded by the viral (+)-strand. The (+)-s...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1023/a:1008022521329
更新日期：1998-01-01 00:00:00
abstract：:In this study, we report the discovery and molecular characterization of a novel mycovirus, Nigrospora oryzae fusarivirus 1 (NoFV1) isolated from the rice-infecting fungus Nigrospora oryzae. Excluding a poly (A) tail, the genome of the virus is 7004 nucleotide (nt) long containing three putative nonoverlapping open re...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/s11262-016-1371-5
更新日期：2016-12-01 00:00:00
abstract：:The infection of humans with Boma disease virus (BDV) is still a matter of debate. In a recent publication, we described a BDV (RW98) isolated from the blood of a psychiatric patient. The RNA of this virus differed more than 5% from that of the widely used strain He/80, which was supposed to represent our laboratory v...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1023/a:1022321920287
更新日期：2003-01-01 00:00:00
abstract：:The Tahyna virus (TAHV) is an important human pathogen in the Bunyaviridae family. To date, only the S and M segments of this virus have been sequenced, but the sequence of the L segment hasn't been established yet. In this study, we sequenced 963 nucleotides of the L segment of TAHV, comprising pre-motif A and motif ...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/s11262-008-0212-6
更新日期：2008-06-01 00:00:00
abstract：:Yellow oat-grass plants (Trisetum flavescens L.) with mild mosaic and pronounced dwarfing symptoms were observed at different locations in the Czech Republic. Electron microscope observations of symptomatic plants revealed the presence of filamentous particles and inclusion bodies characteristic of the family Potyviri...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/s11262-009-0373-y
更新日期：2009-08-01 00:00:00
abstract：:Porcine pegiviruses (PPgV) have been first discovered in serum samples from domestic pigs in Germany in 2016 and then in the USA in 2018. To date, there is no documentation with respect to the presence of PPgVs in domestic pigs in China. Herein, we attempted to determine the presence and prevalence of PPgV in China an...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/s11262-018-1624-6
更新日期：2019-04-01 00:00:00
abstract：:Two ribozyme genes were designed to cut within the VP4 and VP3 sequences of foot and mouth disease virus (FMDV) Asia1 serotype genome. The two genes were synthesized and cloned into pBluescript under the control of the T3 promoter. The ribozyme designed to cut the VP4 gene contained two catalytic sequences targeted to...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/BF01702658
更新日期：1995-01-01 00:00:00
abstract：:Emerging pseudorabies virus (PRV) variant has led to frequent outbreaks of PRV infection among Bartha-K61-vaccinated swine population in Chinese swine farms and caused high mortality in pigs of all age since late 2011. Here, we generated a gE/gI-deleted PRV (rPRVXJ-delgI/gE-EGFP) based on PRV variant strain (PRV-XJ) t...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/s11262-017-1454-y
更新日期：2017-08-01 00:00:00
abstract：:The genomic diversity of Argentine Tospoviruses from different geographical areas, and from several distinct crops was analysed here. For each isolate, RT-PCR, cloning and sequencing of a 450 nt fragment of the N gene were performed. Comparisons of RNA and predicted amino acid sequence identity and similarity were mad...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/BF00366986
更新日期：1996-01-01 00:00:00
abstract：:Bacteriophage T7 RNA polymerase and a derivative containing a nuclear localization signal were transiently expressed in CV-1 cells and were shown to localize to the cytoplasm and nucleus, respectively. A vector was constructed containing T7 promoter and transcription terminator sequences flanking a picornaviral 5' unt...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/BF01702562
更新日期：1992-08-01 00:00:00
abstract：:The complete nucleotide sequences of neuraminidase (NA) of two swine influenza viruses (H1N1) are presented. A/Sw/Quebec/5393/91 (SwQc91) virus, associated with the chronic respiratory disease and A/Sw/ Quebec/192/81 (SwQc81) virus, associated with the acute respiratory disease, were used. The deduced amino acid seque...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1023/a:1007944228967
更新日期：1997-01-01 00:00:00
abstract：:Proteins harbor domains or short linear motifs, which facilitate their functions and interactions. Finding functional motifs in protein sequences could predict the putative cellular roles or characteristics of hypothetical proteins. In this study, we present Shetti-Motif, which is an interactive tool to (i) map UniPro...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/s11262-016-1416-9
更新日期：2017-04-01 00:00:00
abstract：:Although it is known that Cambodia is one of the high endemic area of hepatitis B virus (HBV) infection, molecular characterization of HBV circulating in this country has not been reported. In this study, pre-S gene of HBV from 12 Cambodian patients was sequenced. Phylogenetic analysis based on the pre-S gene sequence...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/s11262-008-0205-5
更新日期：2008-04-01 00:00:00
abstract：:Gallid herpesvirus-1 (GaHV-1), commonly named infectious laryngotracheitis (ILT) virus, causes the respiratory disease in chickens known as ILT. The molecular determinants associated with differences in pathogenicity of GaHV-1 strains are not completely understood, and a comparison of genomic sequences of isolates tha...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/s11262-011-0696-3
更新日期：2012-04-01 00:00:00
abstract：:A bovine viral diarrhea disease virus (BVDV) GS-4 was isolated in Western China form dairy cattle with respiratory disease. Genomic comparison analysis with the 5' half genome sequence encompassing the coding region of N(pro), capsid, and envelope glycoproteins showed that the GS-4 should be classified into BVDV-1b1, ...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/s11262-013-0917-z
更新日期：2013-08-01 00:00:00
abstract：:This study brings the analysis of amino acid sequences of hemagglutinin (HA) from the influenza virus A that can infect a wide variety of birds and mammals. 191 sequences belonging to all known 15 HA subtypes were compared. The emphasis was given on functional sites (receptor-binding cavity with its right and left edg...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1023/a:1014038120979
更新日期：2002-01-01 00:00:00
abstract：:The major immunogenic protein VP2 from a pathogenic field isolate (variant A virus) of infectious bursal disease virus (IBDV) was cloned and sequenced to examine antigenic variations. The VP2 open reading frame consists of 1509 nucleotides and codes for a 503 amino acid protein. Overall, the VP2 amino acid sequence of...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/BF01702563
更新日期：1992-08-01 00:00:00
abstract：:The genome of equine arteritis virus (EAV) produces a 3' coterminal-nested set of six subgenomic (sg) viral RNAs during virus replication cycle, and each set possesses a common leader sequence of 206 nucleotides (nt) in length derived from the 5' end of the viral genome. Given the presence of the leader region within ...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/s11262-004-4589-6
更新日期：2005-01-01 00:00:00
abstract：:The California 99 (Cal99) variant of infectious bronchitis virus (IBV) was first recovered in 1999 from vaccinated broiler chicken flocks in Central California. The S1 hypervariable region of Cal99 genome was most closely related to Arkansas (Ark) serotype viruses. In this study, the complete genome of Cal99 was seque...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/s11262-006-0014-7
更新日期：2007-06-01 00:00:00
abstract：:Currently, virus-based vectors, namely derivatives of the adenovirus, are frequently used in a wide variety of ex vivo or local gene therapeutic applications. However, the efficacy of virus-based vectors in systemic applications is presently still extremely limited. Complex interactions of the various vector types wit...
journal_title：Virus genes
pub_type： 杂志文章,评审
doi：10.1007/s11262-017-1498-z
更新日期：2017-10-01 00:00:00
abstract：:The antigenic variation in Foot and Mouth Disease Virus (FMDV) is very high. The effective strategy to control the Foot and Mouth Disease (FMD) in India which is a habitat of four serotypes O, A, C and Asia 1, is by regular vaccination, using the vaccine strain most suitable for the local situation. India is an endemi...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1023/a:1007984621839
更新日期：1997-01-01 00:00:00
abstract：:The Jumonji C-domain containing protein 6 (JMJD6) has had a convoluted history, and recent reports indicating a multifactorial role in foot-and-mouth disease virus (FMDV) infection have further complicated the functionality of this protein. It was first identified as the phosphatidylserine receptor on the cell surface...
journal_title：Virus genes
pub_type： 杂志文章,评审
doi：10.1007/s11262-017-1449-8
更新日期：2017-06-01 00:00:00
abstract：:DNA viruses as their host cells require a DNA-dependent DNA polymerase (Pol) to faithfully replicate their genomic information. Large eukaryotic DNA viruses as well as bacterial viruses encode a specific Pol equipped with a proofreading 3'-5'-exonuclease, and other replication proteins. All known viral Pol belong to f...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1023/a:1007997609122
更新日期：1998-01-01 00:00:00
abstract：:Serum samples collected from 88 Peruvians with unexplained fever were analyzed for viral sequences using metagenomics. Nucleic acids of anelloviruses, pegivirus A (GBV-C), HIV, Dengue virus, and Oropouche virus were detected. We also characterized from two sera the RNA genomes of new species of partitivirus and dicist...
journal_title：Virus genes
pub_type： 杂志文章
doi：10.1007/s11262-017-1514-3
更新日期：2018-02-01 00:00:00
