Binding of cellular proteins to the leader RNA of equine arteritis virus.

Abstract:

:The genome of equine arteritis virus (EAV) produces a 3' coterminal-nested set of six subgenomic (sg) viral RNAs during virus replication cycle, and each set possesses a common leader sequence of 206 nucleotides (nt) in length derived from the 5' end of the viral genome. Given the presence of the leader region within both genomic and sg mRNAs, it is likely to contain cis-acting signals that may interact with cellular or viral proteins for RNA synthesis. Gel mobility shift assays indicated that proteins in Vero cell cytoplasmic extracts formed complexes with the positive (+) and negative (-) strands of the EAV leader RNA. Several cell proteins with molecular masses ranging from 74 to 31 kDa and 58 to 32 kDa were detected in UV-induced cross-linking assays with the EAV leader RNA (+) and (-) strands, respectively. In both cases, intense bands were observed at the 58-52 kDa molecular weight markers. Results from competition gel mobility shift assays using overlapping cold RNA probes spanning the leader RNA (+) strand indicated that nt 140-206 are not necessary for binding to cell proteins.

journal_name

Virus Genes

journal_title

Virus genes

authors

Archambault D,St-Louis MC,Martin S

doi

10.1007/s11262-004-4589-6

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

121-5

issue

1

eissn

0920-8569

issn

1572-994X

journal_volume

30

pub_type

杂志文章
  • Genetic characterization of bovine coronavirus in Vietnam.

    abstract::A maximum clade credibility tree constructed using the full-length spike (S) and hemagglutinin-esterase genes revealed that Vietnamese Bovine coronavirus (BCoV) strains belong to a single cluster (C1); therefore, they might share a common origin with Cuban and Chinese BCoV strains. The omega values of cluster 1 (C1) a...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-019-01647-1

    authors: Shin J,Tark D,Le VP,Choe S,Cha RM,Park GN,Cho IS,Nga BTT,Lan NT,An DJ

    更新日期:2019-06-01 00:00:00

  • Role of SH3b binding domain in a natural deletion mutant of Kayvirus endolysin LysF1 with a broad range of lytic activity.

    abstract::The spontaneous host-range mutants 812F1 and K1/420 are derived from polyvalent phage 812 that is almost identical to phage K, belonging to family Myoviridae and genus Kayvirus. Phage K1/420 is used for the phage therapy of staphylococcal infections. Endolysin of these mutants designated LysF1, consisting of an N-term...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-017-1507-2

    authors: Benešík M,Nováček J,Janda L,Dopitová R,Pernisová M,Melková K,Tišáková L,Doškař J,Žídek L,Hejátko J,Pantůček R

    更新日期:2018-02-01 00:00:00

  • First complete genome sequences of genogroup V, genotype 3 porcine sapoviruses: common 5'-terminal genomic feature of sapoviruses.

    abstract::Sapoviruses (SaVs) are enteric viruses and have been detected in various mammals. They are divided into multiple genogroups and genotypes based on the entire major capsid protein (VP1) encoding region sequences. In this study, we determined the first complete genome sequences of two genogroup V, genotype 3 (GV.3) SaV ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-017-1481-8

    authors: Oka T,Doan YH,Shimoike T,Haga K,Takizawa T

    更新日期:2017-12-01 00:00:00

  • The characteristics and genome analysis of vB_ApiP_XC38, a novel phage infecting Acinetobacter pittii.

    abstract::Acinetobacter pittii is an important pathogen causing nosocomial infection worldwide. In this study, a multidrug-resistant A. pittii ABC38 was used as host bacterium to isolate the lytic phage vB_ApiP_XC38. The biological characteristics of vB_ApiP_XC38 were studied and the genome was sequenced and analyzed. vB_ApiP_X...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-020-01766-0

    authors: Cheng M,Luo M,Xi H,Zhao Y,Le S,Chen LK,Tan D,Guan Y,Wang T,Han W,Wu N,Zhu T,Gu J

    更新日期:2020-08-01 00:00:00

  • The 2A2 protein of Duck hepatitis A virus type 1 induces apoptosis in primary cell culture.

    abstract::Duck hepatitis A virus type 1, (DHAV-1) 2A2pro, is one of the most highly conserved viral proteins within the DHAV serotypes. However, its effect on host cells is unclear. We predicted that DHAV-1 2A2pro was a GTPase-like protein based on the results of multiple sequence alignment and homologous modeling analysis. Upo...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-016-1364-4

    authors: Cao J,Ou X,Zhu D,Ma G,Cheng A,Wang M,Chen S,Jia R,Liu M,Sun K,Yang Q,Wu Y,Chen X

    更新日期:2016-12-01 00:00:00

  • Variability of E2 protein-coding sequences of bovine viral diarrhea virus in Polish cattle.

    abstract::Bovine viral diarrhea virus (BVDV) belongs to the Pestivirus genus of the Flaviviridae family and has worldwide distribution, being one of the main causes of economic losses in cattle raising. The genome of pestiviruses is a single strand of positive-sense RNA with a length of 12.3 kb, which encodes one open reading f...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-020-01756-2

    authors: Mirosław P,Polak MP

    更新日期:2020-08-01 00:00:00

  • Searching for a new putative cryptic virus in Pinus sylvestris L.

    abstract::Double-stranded RNAs (dsRNAs) were detected in different pine populations in Germany and Hungary. Two dsRNA species of 1.5 and 1.58 kbp, respectively, persisted in the same trees for at least 2 years and their presence was not associated with any symptoms. The dsRNAs were found to sediment in the VLP (virus-like parti...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-005-6874-4

    authors: Veliceasa D,Enünlü N,Kós PB,Köster S,Beuther E,Morgun B,Deshmukh SD,Lukács N

    更新日期:2006-04-01 00:00:00

  • Short communication: isolation and phylogenetic analysis of an avian-origin H3N2 canine influenza virus in dog shelter, China.

    abstract::A H3N2 canine influenza virus, A/canine/Guangdong/3/2011 (H3N2), was isolated from roaming dogs in rural China. Sequence and phylogenetic analysis of eight gene segments revealed that the A/canine/Guangdong/3/2011 (H3N2) was most similar to a recent H3N2 canine influenza virus isolated in cats from South Korea, which ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-013-0882-6

    authors: Su S,Yuan Z,Chen J,Xie J,Li H,Huang Z,Zhang M,Du G,Chen Z,Tu L,Zou Y,Miao J,Wang H,Jia K,Li S

    更新日期:2013-06-01 00:00:00

  • Human immunodeficiency virus type-1 Tat protein induces secretory leukocyte protease inhibitor expression in African green monkey but not human cells.

    abstract::African monkeys are resistant to HIV-1 infection due to intrinsic restriction mechanisms found in their cells. However, although they can be infected by monkey-adapted modified HIV-1 particles that are designed to overcome known restriction factors, virus numbers drop to undetectable levels in immunocompetent animals....

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-020-01731-x

    authors: Özdemir S,Şengez B,Arslanoğlu A

    更新日期:2020-04-01 00:00:00

  • Characterization of a bovine viral diarrhea virus originated from cattle in Gansu Province, China.

    abstract::A bovine viral diarrhea disease virus (BVDV) GS-4 was isolated in Western China form dairy cattle with respiratory disease. Genomic comparison analysis with the 5' half genome sequence encompassing the coding region of N(pro), capsid, and envelope glycoproteins showed that the GS-4 should be classified into BVDV-1b1, ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-013-0917-z

    authors: Gao S,Shao J,Du J,Lin T,Cong G,Zhao F,Chang H,Yin H

    更新日期:2013-08-01 00:00:00

  • A live gI/gE-deleted pseudorabies virus (PRV) protects weaned piglets against lethal variant PRV challenge.

    abstract::Emerging pseudorabies virus (PRV) variant has led to frequent outbreaks of PRV infection among Bartha-K61-vaccinated swine population in Chinese swine farms and caused high mortality in pigs of all age since late 2011. Here, we generated a gE/gI-deleted PRV (rPRVXJ-delgI/gE-EGFP) based on PRV variant strain (PRV-XJ) t...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-017-1454-y

    authors: Yin Y,Xu Z,Liu X,Li P,Yang F,Zhao J,Fan Y,Sun X,Zhu L

    更新日期:2017-08-01 00:00:00

  • A new potyvirus first isolated and identified from Angelica sinensis.

    abstract::A filamentous virus was isolated in Angelica sinensis (Angelica sinensis (Oliv.) Diels) which shows mosaic symptoms on leaves in Minxian, Gansu province, China. According to morphology and molecular biology properties, this virus, which has a flexuous rod-shaped particle about 750 nm in length and 12 nm in width, was ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-009-0361-2

    authors: Zhang Y,Wang R,Wang J,Chang J,Zhang X,Chen T,An L,Xu S

    更新日期:2009-08-01 00:00:00

  • Effects of mutations within the SV40 large T antigen ATPase/p53 binding domain on viral replication and transformation.

    abstract::The simian virus 40 (SV40) large T antigen is a 708 amino-acid protein possessing multiple biochemical activities that play distinct roles in productive infection or virus-induced cell transformation. The carboxy-terminal portion of T antigen includes a domain that carries the nucleotide binding and ATPase activities ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1007941622680

    authors: Peden KW,Srinivasan A,Vartikar JV,Pipas JM

    更新日期:1998-01-01 00:00:00

  • Arenavirus phylogeny: a new insight.

    abstract::Arenaviridae is a worldwide distributed family, of enveloped, single stranded, RNA viruses. The arenaviruses were divided in two major groups (Old World and New World), based on serological properties and genetic data, as well as the geographic distribution. In this study the phylogenetic relationship among the member...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1007993525052

    authors: Albariño CG,Posik DM,Ghiringhelli PD,Lozano ME,Romanowski V

    更新日期:1998-01-01 00:00:00

  • Genetic analysis of the PB1-F2 gene of equine influenza virus.

    abstract::Amino acid and phylogenetic analysis showed that PB1-F2 of H7N7 and H3N8 equine influenza virus (EIV) evolved into two and nine clades, respectively. The PB1-F2 gene of H7N7 EIV strains isolated after 1973 was identified as being replaced by that of H3N8 EIV circulating simultaneously. All H7N7 EIV strains before 1973...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-013-0935-x

    authors: Lu G,Guo W,Qi T,Ma J,Zhao S,Tian Z,Pan J,Zhu C,Wang X,Xiang W

    更新日期:2013-10-01 00:00:00

  • Establishment of mouse leukemia cell lines expressing human CD4/CCR5 using lentiviral vectors.

    abstract::A low-cost rodent model of HIV infection and which presents high application value is an effective tool to investigate HIV infection and pathogenesis. However, development of such a small animal model has been hampered by the unsuitability of rodent cells for HIV-1 replication given that the retrovirus HIV-1 has high ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-016-1423-x

    authors: Li YJ,ZhuGe FY,Zeng CC,He JY,Tan N,Liang J

    更新日期:2017-04-01 00:00:00

  • A common structural core in the internal ribosome entry sites of picornavirus, hepatitis C virus, and pestivirus.

    abstract::Cap-independent translations of viral RNAs of enteroviruses and rhinoviruses, cardioviruses and aphthoviruses, hepatitis A and C viruses (HAV and HCV), and pestivirus are initiated by the direct binding of 40S ribosomal subunits to a cis-acting genetic element termed the internal ribosome entry site (IRES) or ribosome...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/BF00572952

    authors: Le SY,Siddiqui A,Maizel JV Jr

    更新日期:1996-01-01 00:00:00

  • Antigenic variation in Foot and Mouth Disease Virus type Asia 1 isolates circulated during 1993-95 in India.

    abstract::The antigenic variation in Foot and Mouth Disease Virus (FMDV) is very high. The effective strategy to control the Foot and Mouth Disease (FMD) in India which is a habitat of four serotypes O, A, C and Asia 1, is by regular vaccination, using the vaccine strain most suitable for the local situation. India is an endemi...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1007984621839

    authors: Tulasiram P,Tyagi M,Srinivas K,Prabhudas K,Natarajan C,Suryanarayana V

    更新日期:1997-01-01 00:00:00

  • Molecular analysis of the complete genomic sequences of four isolates of Gooseberry vein banding associated virus.

    abstract::The presence of Gooseberry vein banding associated virus (GVBaV), a badnavirus in the family Caulimoviridae, is strongly correlated with gooseberry vein banding disease in Ribes spp. In this study, full-length genomic sequences of four GVBaV isolates from different hosts and geographic regions were determined to be 76...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-011-0614-8

    authors: Xu D,Mock R,Kinard G,Li R

    更新日期:2011-08-01 00:00:00

  • Expression of the X protein of hepatitis B virus in insect cells using recombinant baculoviruses.

    abstract::The baculovirus system was used to express the X protein of human hepatitis B virus (HBV). The X open reading frames (X ORFs) from cloned viral DNA of the HBV subtypes ayw and adr were introduced into the genome of Autographa californica nuclear polyhedrosis virus (AcNPV). The HBV-DNA of subtype adr derived from a hep...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/BF00571930

    authors: Klein R,Schröder CH,Zentgraf H

    更新日期:1991-04-01 00:00:00

  • Prevalence of TT virus and GBV-C infections among patients with liver diseases and the general population in Shanghai, China.

    abstract::To determine the prevalence of TT virus (TTV) and GB virus C/hepatitis G virus (GBV-C) infections among patients with liver disease and the general population in Shanghai, China, we studied 90 patients with liver diseases (acute hepatitis, 28; chronic hepatitis, 27; liver cirrhosis, 20; hepatocellular carcinoma, 15) a...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1008188623062

    authors: Ding X,Mizokami M,Kang LY,Cao K,Orito E,Tanaka Y,Ueda R,Sasaki M

    更新日期:1999-01-01 00:00:00

  • Genetic characterization of a rare H12N3 avian influenza virus isolated from a green-winged teal in Japan.

    abstract::This study reports on the genetic characterization of an avian influenza virus, subtype H12N3, isolated from an Eurasian green-winged teal (Anas crecca) in Japan in 2009. The entire genome sequence of the isolate was analyzed, and phylogenetic analyses were conducted to characterize the evolutionary history of the iso...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-014-1162-9

    authors: Bui VN,Ogawa H,Hussein IT,Hill NJ,Trinh DQ,AboElkhair M,Sultan S,Ma E,Saito K,Watanabe Y,Runstadler JA,Imai K

    更新日期:2015-04-01 00:00:00

  • Genomic characterization of a slow/low maedi visna virus.

    abstract::The complete genomic sequence of a sheep lentivirus isolate that presents a slow/low phenotype in vitro has been determined. The virus, designated P1OLV, was isolated from lung cells of a naturally infected sheep in Portugal. Three overlapping DNA fragments amplified by PCR, and encompassing the entire viral genome we...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/B:VIRU.0000036380.01957.37

    authors: Barros SC,Ramos F,Duarte M,Fagulha T,Cruz B,Fevereiro M

    更新日期:2004-10-01 00:00:00

  • Evolutionary analysis of influenza C virus M genes.

    abstract::The previous study of the 25 hemagglutinin-esterase (HE) glycoprotein genes of influenza C viruses identified four discrete lineages represented by C/Yamagata/26/81, C/Aichi/1/81, C/Aomori/74 and C/Mississippi/80, respectively. Here we compared the M gene sequence among the 24 viruses isolated between 1964 and 1991. A...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1007915215958

    authors: Tada Y,Hongo S,Muraki Y,Sugawara K,Kitame F,Nakamura K

    更新日期:1997-01-01 00:00:00

  • Phylogenetic analysis of hemagglutinin and neuraminidase genes of H9N2 viruses isolated from migratory ducks.

    abstract::Genetic analysis indicated that the pandemic influenza strains derived from wild aquatic birds harbor viruses of 15 hemagglutinin (HA) and 9 neuraminidase (NA) antigenic subtypes. Surveillance studies have shown that H9N2 subtype viruses are worldwide in domestic poultry and could infect mammalian species, including h...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1026304117797

    authors: Liu JH,Okazaki K,Shi WM,Kida H

    更新日期:2003-12-01 00:00:00

  • A human vaccine strain of lamb rotavirus (Chinese) NSP4 gene: complete nucleotide sequence and phylogenetic analyses.

    abstract::A lamb strain of rotavirus has recently been licensed for use in China as a live vaccine to prevent rotavirus diarrhea in children. As rotavirus NSP4, especially the cytotoxic domain alone is considered to be associated with diarrhea, we sequenced gene segment 10, which encodes NSP4, of lamb rotavirus. Comparative ana...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1023491514820

    authors: Mohan KV,Kulkarni S,Glass RI,Zhisheng B,Atreya CD

    更新日期:2003-01-01 00:00:00

  • Sequence characteristics of natural populations of tomato spotted wilt tospovirus infecting flue-cured tobacco in Georgia.

    abstract::Using primers from conserved regions, the nucleocapsid (Nc) gene sequences of naturally occurring tomato spotted wilt tospovirus (TSWV) isolates from flue-cured tobacco (Nicotiana tabacum) grown in several Georgia counties were amplified by immunocapture-reverse transcription-polymerase chain reaction. The resulting a...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1008072825152

    authors: Pappu H,Pappu S,Jain R,Bertrand P,Culbreath A,McPherson R,Csinos A

    更新日期:1998-01-01 00:00:00

  • Vaccinia virus recombinants expressing rabiesvirus glycoprotein protect against rabies.

    abstract::Six recombinants of New York Board of Health (NYBH) vaccinia virus containing cDNA for Challenge Virus Standard (CVS) rabiesvirus glycoprotein (G) were produced by directing gene insertion into the vaccinia thymidine kinase (TK) locus. To regulate expression of G the promoter P7.5 (functions at early and late times po...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/BF00125682

    authors: Esposito J,Brechling K,Baer G,Moss B

    更新日期:1987-11-01 00:00:00

  • Molecular characterization of the env gene from Brazilian field isolates of Bovine leukemia virus.

    abstract::Molecular characterization of Bovine leukemia virus (BLV) isolates from Brazil using the env gene sequences revealed a high conservation of this gene. In most cases the substitutions corresponded to silent transitions. In addition, cystein residues, potential glycosylation sites, neutralization domains and other criti...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-006-0011-x

    authors: Camargos MF,Pereda A,Stancek D,Rocha MA,dos Reis JK,Greiser-Wilke I,Leite RC

    更新日期:2007-06-01 00:00:00

  • Complete genome sequence of a novel Plum pox virus strain W isolate determined by 454 pyrosequencing.

    abstract::The near-complete (99.7 %) genome sequence of a novel Russian Plum pox virus (PPV) isolate Pk, belonging to the strain Winona (W), has been determined by 454 pyrosequencing with the exception of the thirty-one 5'-terminal nucleotides. This region was amplified using 5'RACE kit and sequenced by the Sanger method. Genom...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-013-0946-7

    authors: Sheveleva A,Kudryavtseva A,Speranskaya A,Belenikin M,Melnikova N,Chirkov S

    更新日期:2013-10-01 00:00:00