Abstract:
:Using primers from conserved regions, the nucleocapsid (Nc) gene sequences of naturally occurring tomato spotted wilt tospovirus (TSWV) isolates from flue-cured tobacco (Nicotiana tabacum) grown in several Georgia counties were amplified by immunocapture-reverse transcription-polymerase chain reaction. The resulting amplicons were cloned and sequenced. Sequence analyses showed a high degree of sequence conservation among the Nc genes of the tobacco isolates, and those reported from other parts of the world. However, distinct differences that were unique to these tobacco isolates as well as the previously studied peanut, tomato and pepper isolates from Georgia were present. The Georgia isolates formed a distinct cluster that was clearly resolved from the rest of the TSWV isolates based on sequence phylograms.
journal_name
Virus Genesjournal_title
Virus genesauthors
Pappu H,Pappu S,Jain R,Bertrand P,Culbreath A,McPherson R,Csinos Adoi
10.1023/a:1008072825152subject
Has Abstractpub_date
1998-01-01 00:00:00pages
169-77issue
2eissn
0920-8569issn
1572-994Xjournal_volume
17pub_type
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