Sequence characteristics of natural populations of tomato spotted wilt tospovirus infecting flue-cured tobacco in Georgia.

Abstract:

:Using primers from conserved regions, the nucleocapsid (Nc) gene sequences of naturally occurring tomato spotted wilt tospovirus (TSWV) isolates from flue-cured tobacco (Nicotiana tabacum) grown in several Georgia counties were amplified by immunocapture-reverse transcription-polymerase chain reaction. The resulting amplicons were cloned and sequenced. Sequence analyses showed a high degree of sequence conservation among the Nc genes of the tobacco isolates, and those reported from other parts of the world. However, distinct differences that were unique to these tobacco isolates as well as the previously studied peanut, tomato and pepper isolates from Georgia were present. The Georgia isolates formed a distinct cluster that was clearly resolved from the rest of the TSWV isolates based on sequence phylograms.

journal_name

Virus Genes

journal_title

Virus genes

authors

Pappu H,Pappu S,Jain R,Bertrand P,Culbreath A,McPherson R,Csinos A

doi

10.1023/a:1008072825152

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

169-77

issue

2

eissn

0920-8569

issn

1572-994X

journal_volume

17

pub_type

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