Abstract:
:Japanese encephalitis (JE), which is a mosquito-borne arboviral infection, is the leading cause of viral encephalitis in Asian countries. The causative agent of JE is Japanese encephalitis virus (JEV), in which the predominant genotype has changed from genotype III (G III) to genotype I (G I). However, a method for the rapid differentiation between JEV G I and G III remains unavailable. This study aimed to establish a rapid JEV genotyping method using reverse transcription loop-mediated isothermal amplification (RT-LAMP). An Spe I site, which was located in the target sequence (C gene) of JEV G III strains but not in JEV G I strains, was selected as the RT-LAMP target. After testing 64 specimens, results showed that RT-LAMP can detect and differentiate JEV G I and G III specifically. Thus, a novel RT-LAMP system for the rapid detection and differentiation of JEV G I and G III was developed successfully.
journal_name
Virus Genesjournal_title
Virus genesauthors
Zhang L,Cao S,Wu R,Zhu S,Liu H,Yuan L,Shi S,Zhang D,Huang X,Wen X,Wen Y,Yan Q,Huang Y,Ma Xdoi
10.1007/s11262-014-1158-5subject
Has Abstractpub_date
2015-04-01 00:00:00pages
231-7issue
2eissn
0920-8569issn
1572-994Xjournal_volume
50pub_type
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