Detection and differentiation of Japanese encephalitis virus genotype I and genotype III by reverse transcription loop-mediated isothermal amplification combined with restriction fragment length polymorphism.

Abstract:

:Japanese encephalitis (JE), which is a mosquito-borne arboviral infection, is the leading cause of viral encephalitis in Asian countries. The causative agent of JE is Japanese encephalitis virus (JEV), in which the predominant genotype has changed from genotype III (G III) to genotype I (G I). However, a method for the rapid differentiation between JEV G I and G III remains unavailable. This study aimed to establish a rapid JEV genotyping method using reverse transcription loop-mediated isothermal amplification (RT-LAMP). An Spe I site, which was located in the target sequence (C gene) of JEV G III strains but not in JEV G I strains, was selected as the RT-LAMP target. After testing 64 specimens, results showed that RT-LAMP can detect and differentiate JEV G I and G III specifically. Thus, a novel RT-LAMP system for the rapid detection and differentiation of JEV G I and G III was developed successfully.

journal_name

Virus Genes

journal_title

Virus genes

authors

Zhang L,Cao S,Wu R,Zhu S,Liu H,Yuan L,Shi S,Zhang D,Huang X,Wen X,Wen Y,Yan Q,Huang Y,Ma X

doi

10.1007/s11262-014-1158-5

subject

Has Abstract

pub_date

2015-04-01 00:00:00

pages

231-7

issue

2

eissn

0920-8569

issn

1572-994X

journal_volume

50

pub_type

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