Detection and complete genome characteristics of Posavirus 1 from pigs in China.

Abstract:

:Porcine stool-associated RNA virus 1 (Posavirus 1) is a novel member of picornaviruses and first identified from fecal samples of 30-day-old pigs with diarrhea in USA in 2011. To evaluate the existence of Posavirus 1 in swine herds, 118 clinical samples from diarrheal pigs and 31 fecal swabs from healthy pigs were collected and detected by reverse transcription-polymerase chain reaction (RT-PCR) using Posavirus 1-specific primers. Only five fecal samples from diarrheal pigs on two swine farms were positive for Posavirus 1. The complete genome sequences [excluding poly (A) tail] of two representative isolates SDQD-25 and HBTS-11 are determined and consist of 9840 and 9819 nucleotides in length, and encode one putative polyprotein of 3070 and 2952 amino acids, respectively. They share 90.3% homology with each other and 81.3-95.4% homologies with American Posavirus 1 isolates or strains at the nucleotide sequence level. The phylogenetic analysis based on the entire genomes of reference picornavirus strains or isolates showed SDQD-25, HBTS-11 cluster together with American Posavirus 1 isolates or strains, yet are clearly distant from the other picornaviruses. The complete genome sequences of Chinese Posavirus 1 isolates will enrich the information of Posavirus 1 sequence database and further expedite posavirus research on the genetic diversity, epidemiology, and evolution in China.

journal_name

Virus Genes

journal_title

Virus genes

authors

Chen J,Lu M,Ma T,Cao L,Zhu X,Zhang X,Shi D,Shi H,Liu J,Feng L

doi

10.1007/s11262-017-1512-5

subject

Has Abstract

pub_date

2018-02-01 00:00:00

pages

145-148

issue

1

eissn

0920-8569

issn

1572-994X

pii

10.1007/s11262-017-1512-5

journal_volume

54

pub_type

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