A synthetic peptide derived from domain III envelope glycoprotein of Dengue virus induces neutralizing antibody.

Abstract:

:Dengue virus (DENV) is an arthropod-borne human pathogen that represents a severe public health threat in both endemic and non-endemic regions. So far, there is no licensed vaccine or specific drugs available for dengue fever. A fifteen-amino-acid-long peptide that includes the NGR motif was chemically synthesized and conjugated with keyhole limpet hemocyanin. A standard immunization protocol was followed for the production of polyclonal antibodies by immunizing rabbits against the synthetic peptide. The immune response elicited high-titer polyclonal antibodies with the reactivity of the anti-peptide antibody against both synthetic peptide and four serotypes of DENV confirmed by DOT-ELISA. Neutralizing activity of anti-peptide antibody was found to be cross-reactive and effective resulting in 60% reduction of infectivity at 1:200 dilution in all four serotypes of DENV. Our findings have the potential to further improve our understanding of virus-host interactions and provide new insights into neutralizing antibodies and could also be used as a drug target.

journal_name

Virus Genes

journal_title

Virus genes

authors

Mary JA,Jittmittraphap A,Chattanadee S,Leaungwutiwong P,Shenbagarathai R

doi

10.1007/s11262-017-1508-1

subject

Has Abstract

pub_date

2018-02-01 00:00:00

pages

25-32

issue

1

eissn

0920-8569

issn

1572-994X

pii

10.1007/s11262-017-1508-1

journal_volume

54

pub_type

杂志文章
  • Cloning of E6 and E7 genes of human papilloma virus type 18 and transformation potential of E7 gene and its mutants.

    abstract::E6 and E7 genes of human papilloma virus type 18 have been subcloned from plasmid pC7, carrying an insert of DNA from squamous cell carcinoma of cervix. Both genes in comparison to prototype variant contain one mutation that changes asparagine to leucine. In the case of E6 gene this mutation is mapped in codon 129, in...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1008020719309

    authors: Laassri M,Gul'ko L,Vinokurova S,Kisseljova N,Veiko V,Kisseljov F

    更新日期:1999-01-01 00:00:00

  • Cloning and characterization of a totivirus double-stranded RNA from the plant pathogenic fungus, Helicobasidium mompa Tanaka.

    abstract::Virus-like particles (VLPs, named HmTV1-17), about 40 nm in diameter were found in the violet root rot fungus Helicobasidium mompa Tanaka strain No. 17, which had been isolated from an apple tree. Purified preparations of HmTV1-17 contained two species of double-stranded RNA (dsRNA), designated 17L and 17S. cDNAs were...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1024453111809

    authors: Nomura K,Osaki H,Iwanami T,Matsumoto N,Ohtsu Y

    更新日期:2003-05-01 00:00:00

  • Identification of specific amino acid residues of adenovirus 12 E1A involved in transformation and p300 binding.

    abstract::The early region 1A (E1A) gene of highly oncogenic adenovirus type 12 (Ad12) was analyzed for transforming activity and protein binding using specific mutations. The Ad12 E1A proteins were found to bind p300 protein mainly within the CR1 region, although mutations that affect both p300 binding and transformation were ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1007967009156

    authors: Sawada Y,Ishino M,Miura K,Ohtsuka E,Fujinaga K

    更新日期:1997-01-01 00:00:00

  • Genomic analysis of a recombinant NADC30-like porcine reproductive and respiratory syndrome virus in china.

    abstract::Recently, NADC30-like porcine reproductive and respiratory syndrome viruses (PRRSVs), which are genetically similar to the NADC30 strain isolated in the United States of America in 2008, have become prevalent in China. Here, a novel variant PRRSV strain named HNhx was successfully isolated on porcine alveolar macropha...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-017-1516-1

    authors: Wang LJ,Wan B,Guo Z,Qiao S,Li R,Xie S,Chen XX,Zhang G

    更新日期:2018-02-01 00:00:00

  • Genetic analysis of the PB1-F2 gene of equine influenza virus.

    abstract::Amino acid and phylogenetic analysis showed that PB1-F2 of H7N7 and H3N8 equine influenza virus (EIV) evolved into two and nine clades, respectively. The PB1-F2 gene of H7N7 EIV strains isolated after 1973 was identified as being replaced by that of H3N8 EIV circulating simultaneously. All H7N7 EIV strains before 1973...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-013-0935-x

    authors: Lu G,Guo W,Qi T,Ma J,Zhao S,Tian Z,Pan J,Zhu C,Wang X,Xiang W

    更新日期:2013-10-01 00:00:00

  • Phylogenetic analysis of hop and grapevine isolates of hop stunt viroid supports a grapevine origin for hop stunt disease.

    abstract::We have examined sequence variability among nine isolates of hop stunt viroid (HSVd) collected from hop gardens in Tohoku district in Japan, the only area in the world where hop stunt disease is endemic. Six different consensus and one-consensus sequences as well as 12 sequence variants were detected in the nine HSVd-...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1008182302704

    authors: Sano T,Mimura R,Ohshima K

    更新日期:2001-01-01 00:00:00

  • Sequence analysis of the variable VP2 gene of infectious bursal disease viruses passaged in Vero cells.

    abstract::Korean field infectious bursal disease viruses (IBDVs) were isolated from IBDV suspected commercial chickens. A previous study revealed that these IBDV field isolates were virulent or very virulent IBDVs. The isolates were passaged three times in the chorioallantoic membrane of specific-pathogen-free embryonated chick...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/b:viru.0000025776.52661.b0

    authors: Kwon HM,Kim SJ

    更新日期:2004-04-01 00:00:00

  • Satsuma dwarf and related viruses belong to a new lineage of plant picorna-like viruses.

    abstract::Satsuma dwarf virus (SDV) and two closely related viruses, Citrus mosaic (CiMV), and Naval orange infectious mottling (NIMV), seriously affect citrus varieties grown in Japan and East Asia. All three viruses have icosahedral particles built of two proteins encapsidating two single-stranded genomic RNAs. The natural mo...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1011131328951

    authors: Karasev AV,Han SS,Iwanami T

    更新日期:2001-01-01 00:00:00

  • Complete nucleotide sequence and genome organization of a Chinese isolate of Tobacco vein distorting virus.

    abstract::Tobacco bushy top disease is caused by tobacco bushy top virus (TBTV, a member of the genus Umbravirus) which is dependent on tobacco vein-distorting virus (TVDV) to act as a helper virus encapsidating TBTV and enabling its transmission by aphids. Isometric virions from diseased tobacco plants were purified and diseas...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-010-0524-1

    authors: Mo XH,Chen ZB,Chen JP

    更新日期:2010-12-01 00:00:00

  • Loss of linkage disequilibrium and accelerated protein divergence in duplicated cytomegalovirus chemokine genes.

    abstract::Human CMV (hCMV) encodes several captured chemokine ligand and chemokine receptor genes that may play a role in immune evasion. The adjacent viral alpha-chemokine genes UL146 and UL147 appear to have duplicated subsequent to a recent gene capture event. Sequence data from multiple hCMV isolates suggest accelerated pro...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-005-2201-3

    authors: Arav-Boger R,Zong JC,Foster CB

    更新日期:2005-08-01 00:00:00

  • Transcription analysis of porcine circovirus (PCV).

    abstract::This study focuses on gene expression of porcine circovirus (PCV) in order to identify viral genes and their corresponding mRNA transcripts. By northern blot analysis, the existence of three mRNAs could be demonstrated. Two mRNAs are encoded by the viral (-)-strand and one is encoded by the viral (+)-strand. The (+)-s...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1008022521329

    authors: Mankertz J,Buhk HJ,Blaess G,Mankertz A

    更新日期:1998-01-01 00:00:00

  • Identification and characterization of odv-e25 of Spodoptera litura multicapsid nucleopolyhedrovirus.

    abstract::Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) odv-e25 is 684 bp long, potentially encoding 227 amino acids with a predicted molecular weight of 24.9 kDa. Homology analysis indicated that SpltMNPV ODV-E25 has 35-65% amino acid identity with that of other known baculoviruses. RT-PCR results revealed that...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-005-5840-5

    authors: Li Z,Pan L,Yu H,Li S,Zhang G,Pang Y

    更新日期:2006-02-01 00:00:00

  • Characterization of white spot syndrome virus VP52B and its interaction with VP26.

    abstract::White spot syndrome virus (WSSV) is one of the major pathogens of cultured shrimp. Identification of envelope protein interactions has become a central issue for the understanding of WSSV assembly. In this paper, WSSV envelope protein VP52B was fused with GST-tag and expressed in Escherichia coli BL-21(DE3). Immunogol...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-014-1126-0

    authors: Lin F,Jie Z,Hou L,Li F,Yang F

    更新日期:2015-02-01 00:00:00

  • Characterization of an early gene (wsv477) from shrimp white spot syndrome virus (WSSV).

    abstract::White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. The viral early genes play a key role in DNA replication and virus proliferation. In this study, a WSSV gene (wsv477) encoding 208 amino acid peptides was characterized as an early gene. The temporal analysis showe...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-006-0053-0

    authors: Han F,Xu J,Zhang X

    更新日期:2007-04-01 00:00:00

  • The 2A2 protein of Duck hepatitis A virus type 1 induces apoptosis in primary cell culture.

    abstract::Duck hepatitis A virus type 1, (DHAV-1) 2A2pro, is one of the most highly conserved viral proteins within the DHAV serotypes. However, its effect on host cells is unclear. We predicted that DHAV-1 2A2pro was a GTPase-like protein based on the results of multiple sequence alignment and homologous modeling analysis. Upo...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-016-1364-4

    authors: Cao J,Ou X,Zhu D,Ma G,Cheng A,Wang M,Chen S,Jia R,Liu M,Sun K,Yang Q,Wu Y,Chen X

    更新日期:2016-12-01 00:00:00

  • Evolution of surface and nonstructural-1 genes of influenza B viruses isolated in the Province of Québec, Canada, during the 1998-2001 period.

    abstract::After 2 minor winter seasons, influenza B viruses were predominantly isolated in the Province of Quebec, Canada, during the 2000-2001 season representing 74% of laboratory-confirmed influenza viruses. We performed an antigenic study of the hemagglutinin (HA) protein and a molecular characterization of the HA1 region, ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1025768308631

    authors: Abed Y,Coulthart MB,Li Y,Boivin G

    更新日期:2003-10-01 00:00:00

  • A new potyvirus first isolated and identified from Angelica sinensis.

    abstract::A filamentous virus was isolated in Angelica sinensis (Angelica sinensis (Oliv.) Diels) which shows mosaic symptoms on leaves in Minxian, Gansu province, China. According to morphology and molecular biology properties, this virus, which has a flexuous rod-shaped particle about 750 nm in length and 12 nm in width, was ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-009-0361-2

    authors: Zhang Y,Wang R,Wang J,Chang J,Zhang X,Chen T,An L,Xu S

    更新日期:2009-08-01 00:00:00

  • Oncolytic viruses: adenoviruses.

    abstract::Tumor-selectively replicating (oncolytic) viruses are promising tools for therapy of solid cancers and have been initially developed to achieve potent tumor lysis with acceptable side effects on healthy tissue. However, in recent years, oncolytic viruses have been recognized as therapeutic vehicles exhibiting multipro...

    journal_title:Virus genes

    pub_type: 杂志文章,评审

    doi:10.1007/s11262-017-1488-1

    authors: Niemann J,Kühnel F

    更新日期:2017-10-01 00:00:00

  • Genomic comparison of an avirulent strain of Orf virus with that of a virulent wild type isolate reveals that the Orf virus G2L gene is non-essential for replication.

    abstract::The genomic structure of two strains of orf virus (OV), a field isolate (MRI-Scab) which has never been passaged in cell culture, and a multiple-passage cell culture-adapted strain (Orf-11) were compared. The Orf-11 genome is approximately 8.0 kb longer than that of the MRI-Scab due to a duplication of the right-hand ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1008117127729

    authors: McInnes CJ,Wood AR,Nettleton PE,Gilray JA

    更新日期:2001-03-01 00:00:00

  • Comparison of hepatitis B X gene mutation between patients with hepatocellular carcinoma and patients with chronic hepatitis B.

    abstract::Hepatitis B virus (HBV), a major causative agent of hepatocellular carcinoma (HCC), encodes an oncogenic X protein (HBx) that transcriptionally activates multiple viral and cellular promoters. The present study aimed to identify the specific gene mutation related to the clinical outcome of HCC. Seventy-two HBV-infecte...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-010-0557-5

    authors: Fan W,Shi B,Wei H,Du G,Song S

    更新日期:2011-04-01 00:00:00

  • Analysis of codon usage in Newcastle disease virus.

    abstract::In this study, the relative synonymous codon usage (RSCU) values, effective number of codon (ENC) values, nucleotide contents, and dinucleotide were used to investigate codon usage pattern of each protein-coding gene and genome among 31 Newcastle disease virus (NDV) isolates. The result shows that the overall extent o...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-011-0574-z

    authors: Wang M,Liu YS,Zhou JH,Chen HT,Ma LN,Ding YZ,Liu WQ,Gu YX,Zhang J

    更新日期:2011-04-01 00:00:00

  • Nucleotide sequence of the genome segment encoding nonstructural protein NS1 of bluetongue virus serotype 20 from Australia.

    abstract::The nucleotide sequence of the genome segment (S6) encoding the nonstructural protein NS1 of an Australian isolate of bluetongue virus serotype 20 (BTV 20) has been determined from a series of overlapping cDNA clones synthesized using two terminal 15-mer oligonucleotides as primers. The gene consists of 1769 nucleotid...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/BF01703087

    authors: Cowley JA

    更新日期:1992-11-01 00:00:00

  • Nuclear localization signals of varicella zoster virus ORF4.

    abstract::The varicella zoster virus (VZV) ORF4 protein, one of immediate-early genes protein, is associated with the tegument in purified virions. ORF4 protein functions at both transcriptional and post-transcriptional levels, present during different phase of whole VZV life cycle. ORF4 protein acts as a nucleocytoplasm shuttl...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-013-1006-z

    authors: Huang Y,Zhang J,Halawa MA,Yao S

    更新日期:2014-04-01 00:00:00

  • The PDZ domain binding motif (PBM) of human T-cell leukemia virus type 1 Tax can be substituted by heterologous PBMs from viral oncoproteins during T-cell transformation.

    abstract::Several tumor viruses, such as human T-cell leukemia virus (HTLV), human papilloma virus (HPV), human adenovirus, have high-oncogenic and low-oncogenic subtypes, and such subtype-specific oncogenesis is associated with the PDZ-domain binding motif (PBM) in their transforming proteins. HTLV-1, the causative agent of ad...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-009-0447-x

    authors: Aoyagi T,Takahashi M,Higuchi M,Oie M,Tanaka Y,Kiyono T,Aoyagi Y,Fujii M

    更新日期:2010-04-01 00:00:00

  • Trends of subtype variation of human immunodeficiency virus type 1 in Zhejiang Province, China.

    abstract::The epidemic characteristics of human immunodeficiency virus type 1 (HIV-1) in Zhejiang Province have not been systematically identified. This study presented a dynamic analysis of HIV-1 subtype variation in Zhejiang from 2004 to 2008, based on the surveillance of molecular epidemiology or drug resistance. CRF01_AE wa...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-014-1136-y

    authors: Yao Y,Xin R,Yang J,Xu Y,Zhang J,Xia Y,Pan X,Guo Z,Song Y

    更新日期:2015-02-01 00:00:00

  • The genome sequence of an H11N2 avian influenza virus from a Thick-billed Murre (Uria lomvia) shows marine-specific and regional patterns of relationships to other viruses.

    abstract::Influenza A viruses infect a range of host species, including various mammals and more than 100 species of birds. For avian influenza viruses (AIV), prevalence varies between different groups of birds, with waterfowl showing the highest prevalence. We have sequenced the complete genome of A/Thick-billed Murre/Newfound...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-010-0504-5

    authors: Granter A,Wille M,Whitney H,Robertson GJ,Ojkic D,Lang AS

    更新日期:2010-10-01 00:00:00

  • Detection, differentiation and phylogenetic analysis of cucumber mosaic virus isolates from cucurbits in the northwest region of Iran.

    abstract::One hundred and twenty three cucurbit samples with one or more symptoms of leaf mosaic, leaf distortion, fruit mosaic, stunting, mottling and yellowing were collected from several locations in the northwest region of Iran. Screening by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with a cucum...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-005-6912-2

    authors: Bashir NS,Kalhor MR,Zarghani SN

    更新日期:2006-06-01 00:00:00

  • The evolution of small DNA viruses of eukaryotes: past and present considerations.

    abstract::Historically, viral evolution has often been considered from the perspective of the ability of the virus to maintain viral pathogenic fitness by causing disease. A predator-prey model has been successfully applied to explain genetically variable quasi-species of viruses, such as influenza virus and human immunodeficie...

    journal_title:Virus genes

    pub_type: 杂志文章,评审

    doi:10.1007/BF01728663

    authors: Shadan FF,Villarreal LP

    更新日期:1995-01-01 00:00:00

  • Detection by next generation sequencing of a multi-segmented viral genome from sugarcane associated with Ramu stunt disease.

    abstract::Ramu stunt disease of sugarcane was first reported in Papua New Guinea in the mid 1980s. The disease can reduce sugarcane yields significantly and causes severe stunting and mortality in highly susceptible cultivars. The causal agent of Ramu stunt has been investigated but its characterization has not been completed. ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-015-1279-5

    authors: Mollov D,Maroon-Lango C,Kuniata L

    更新日期:2016-02-01 00:00:00

  • First detection and complete genome sequence of Deformed wing virus in Chilean honeybees.

    abstract::Deformed wing virus (DWV) is one of the most common viruses affecting honey bee specimens. Although the presence of DWV has been reported in many countries, there is no data of the current situation in Chile. In this report, we detected the presence of DWV in apiaries from two different locations in central Chile. Fur...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-012-0791-0

    authors: Barriga GP,Cifuentes-Muñoz N,Rivera PA,Gutierrez M,Shmaryahu A,Valenzuela PD,Engel EA

    更新日期:2012-12-01 00:00:00