Genomic characterization of Molluscum contagiosum virus type 1: identification of the repetitive DNA sequences in the viral genome.

Abstract:

:The genomes (188 kbp) of the prototype Molluscum contagiosum virus type 1 (MCV-1) and a variant strain (MCV-1v) were characterized by construction of the physical maps of the viral DNA for the restriction enzymes BamHI, ClaI, EcoRI, and HindIII using a defined gene library harboring the DNA sequences of the MCV-1 genome and by DNA-DNA hybridizations. It was found that the genomes of both MCV strains are identical, with the exception of very few changes in the DNA fragmentation patterns of restriction endonuclease BamHI as a consequence of naturally occurring nucleotide exchanges in the genome of the variant strain. Detailed hybridization experiments revealed the existence of repetitive DNA sequences, which are located within the terminal regions of the viral genome at the map coordinates 0 to 0.027 and 0.973 to 1.

journal_name

Virus Genes

journal_title

Virus genes

authors

Bugert J,Rösen-Wolff A,Darai G

doi

10.1007/BF00125128

subject

Has Abstract

pub_date

1989-11-01 00:00:00

pages

159-73

issue

2

eissn

0920-8569

issn

1572-994X

journal_volume

3

pub_type

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